SSGCID: Target Page

BupsA.00089.a

Putrescine-binding periplasmic protein

CENTER ID:	BupsA.00089.a
ORGANISM:	Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE:	Melioidosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.00089.a.A2.GE30884	Mature protein( BupsA.00089.a )		23	364

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.00089.a.A2.PW30772	Mature protein( BupsA.00089.a )		23	364

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|320372.6.peg.2414
RefSeq:	YP_333705.1
UniProt:	Q3JRU8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKAKVAGRLT ALALCAGATV AAAKDTQLNV YNWSDYIAKD TIPNFEKQTG VKVRYDNYDS DDTLQAKLLT GNSGYDIVVP TSNYAGKQIQ AGIFTPLDKS KLPNLKYLDA QLMALVAGAD PGNKYVVPWA YGTTGLGYNV DKAQKVLGKV PLDNWDILFK PENLSKLKTC GVSVLDAPDQ MFAATLHYIG KDPMSTNPAD YQAAMQVLKK IRPYITQFNS SGYINDMVGG DICFAFGWSG DVVIAKHRAL EAKKPYKLEY YVPKGGAPVW FDVMAIPKDA KNKDAALQWI NYIEDPKVHA SITNAVYYPS ANAQARKYVR PDVANDPAVY PPPDVVKTLF LLKPLPPEIQ RLQTRLWTEL KSGR

NT Sequence

ATGAAGGCAA AGGTGGCGGG CCGGTTGACG GCACTTGCGC TCTGCGCGGG CGCGACGGTG GCGGCGGCGA AGGATACGCA GCTCAACGTC TATAACTGGT CGGACTACAT TGCGAAGGAC ACGATCCCGA ACTTCGAGAA GCAGACGGGC GTCAAGGTCC GCTACGACAA CTACGACAGC GACGACACGC TGCAGGCGAA GCTTTTGACC GGCAACTCGG GCTACGACAT CGTCGTGCCG ACGAGCAACT ACGCGGGCAA GCAGATCCAG GCCGGCATCT TCACGCCGCT CGACAAGTCG AAGCTGCCGA ACCTCAAGTA TCTCGATGCG CAACTGATGG CGCTCGTCGC GGGCGCCGAT CCGGGCAACA AGTACGTGGT GCCCTGGGCG TATGGCACGA CGGGCCTCGG CTACAACGTC GACAAGGCGC AGAAGGTGCT CGGCAAGGTG CCGCTCGACA ACTGGGACAT CCTGTTCAAG CCGGAGAACC TCTCGAAGCT GAAGACGTGC GGCGTGTCGG TGCTCGACGC GCCGGACCAG ATGTTCGCGG CGACGCTGCA CTACATCGGC AAGGATCCGA TGAGCACGAA CCCGGCCGAC TACCAGGCCG CGATGCAGGT GCTCAAGAAG ATCCGCCCGT ACATCACGCA GTTCAACTCG TCGGGCTACA TCAACGACAT GGTCGGCGGC GACATCTGCT TCGCGTTCGG CTGGTCGGGC GACGTCGTGA TCGCGAAGCA CCGCGCGCTC GAGGCGAAGA AGCCGTACAA GCTCGAGTAC TACGTGCCGA AGGGCGGCGC GCCCGTATGG TTCGACGTGA TGGCGATCCC GAAGGACGCG AAGAACAAGG ACGCCGCGCT TCAATGGATC AACTACATCG AGGATCCGAA GGTTCACGCG TCGATCACGA ACGCGGTCTA CTACCCGAGC GCGAACGCGC AAGCGCGCAA GTACGTGCGC CCGGATGTCG CGAACGACCC GGCCGTGTAC CCGCCTCCGG ACGTCGTGAA GACGCTGTTC CTGCTCAAGC CGCTGCCGCC CGAGATCCAG CGCCTGCAGA CGCGTCTTTG GACCGAGCTG AAATCGGGAC GC

Details for BupsA.00089.a.A2.GE30884

HARVESTED ON:	11/23/2010
SEQUENCED ON:	12/6/2010
EXPECTED MW:	40kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MGTLEAQTQG PGSMAKDTQL NVYNWSDYIA KDTIPNFEKQ TGVKVRYDNY DSDDTLQAKL LTGNSGYDIV VPTSNYAGKQ IQAGIFTPLD KSKLPNLKYL DAQLMALVAG ADPGNKYVVP WAYGTTGLGY NVDKAQKVLG KVPLDNWDIL FKPENLSKLK TCGVSVLDAP DQMFAATLHY IGKDPMSTNP ADYQAAMQVL KKIRPYITQF NSSGYINDMV GGDICFAFGW SGDVVIAKHR ALEAKKPYKL EYYVPKGGAP VWFDVMAIPK DAKNKDAALQ WINYIEDPKV HASITNAVYY PSANAQARKY VRPDVANDPA VYPPPDVVKT LFLLKPLPPE IQRLQTRLWT ELKSGR

Validated NT Sequence

tagcgtcccg atttcagctc ggtccaaaga cgcgtctgca ggcgctggat ctcgggcggc agcggcttga gcaggaacag cgtcttcacg acgtccggag gcgggtacac ggccgggtcg ttcgcgacat ccgggcgcac gtacttgcgc gcttgcgcgt tcgcgctcgg gtagtagacc gcgttcgtga tcgacgcgtg aaccttcgga tcctcgatgt agttgatcca ttgaagcgcg gcgtccttgt tcttcgcgtc cttcgggatc gccatcacgt cgaaccatac gggcgcgccg cccttcggca cgtagtactc gagcttgtac ggcttcttcg cctcgagcgc gcggtgcttc gcgatcacga cgtcgcccga ccagccgaac gcgaagcaga tgtcgccgcc gaccatgtcg ttgatgtagc ccgacgagtt gaactgcgtg atgtacgggc ggatcttctt gagcacctgc atcgcggcct ggtagtcggc cgggttcgtg ctcatcggat ccttgccgat gtagtgcagc gtcgccgcga acatctggtc cggcgcgtcg agcaccgaca cgccgcacgt cttcagcttc gagaggttct ccggcttgaa caggatgtcc cagttgtcga gcggcacctt gccgagcacc ttctgcgcct tgtcgacgtt gtagccgagg cccgtcgtgc catacgccca gggcaccacg tacttgttgc ccggatcggc gcccgcgacg agcgccatca gttgcgcatc gagatacttg aggttcggca gcttcgactt gtcgagcggc gtgaagatgc cggcctggat ctgcttgccc gcgtagttgc tcgtcggcac gacgatgtcg tagcccgagt tgccggtcaa aagcttcgcc tgcagcgtgt cgtcgctgtc gtagttgtcg tagcggacct tgacgcccgt ctgcttctcg aagttcggga tcgtgtcctt cgcaatgtag tccgaccagt tatagacgtt gagctgcgta tccttcgcca tcgaaccagg accctgggtc tgagcttcca gggtacccat atga

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMAKDTQLNV YNWSDYIAKD TIPNFEKQTG VKVRYDNYDS DDTLQAKLLT GNSGYDIVVP TSNYAGKQIQ AGIFTPLDKS KLPNLKYLDA QLMALVAGAD PGNKYVVPWA YGTTGLGYNV DKAQKVLGKV PLDNWDILFK PENLSKLKTC GVSVLDAPDQ MFAATLHYIG KDPMSTNPAD YQAAMQVLKK IRPYITQFNS SGYINDMVGG DICFAFGWSG DVVIAKHRAL EAKKPYKLEY YVPKGGAPVW FDVMAIPKDA KNKDAALQWI NYIEDPKVHA SITNAVYYPS ANAQARKYVR PDVANDPAVY PPPDVVKTLF LLKPLPPEIQ RLQTRLWTEL KSGR

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcgaaggat acgcagctca acgtctataa ctggtcggac tacattgcga aggacacgat cccgaacttc gagaagcaga cgggcgtcaa ggtccgctac gacaactacg acagcgacga cacgctgcag gcgaagcttt tgaccggcaa ctcgggctac gacatcgtcg tgccgacgag caactacgcg ggcaagcaga tccaggccgg catcttcacg ccgctcgaca agtcgaagct gccgaacctc aagtatctcg atgcgcaact gatggcgctc gtcgcgggcg ccgatccggg caacaagtac gtggtgccct gggcgtatgg cacgacgggc ctcggctaca acgtcgacaa ggcgcagaag gtgctcggca aggtgccgct cgacaactgg gacatcctgt tcaagccgga gaacctctcg aagctgaaga cgtgcggcgt gtcggtgctc gacgcgccgg accagatgtt cgcggcgacg ctgcactaca tcggcaagga tccgatgagc acgaacccgg ccgactacca ggccgcgatg caggtgctca agaagatccg cccgtacatc acgcagttca actcgtcggg ctacatcaac gacatggtcg gcggcgacat ctgcttcgcg ttcggctggt cgggcgacgt cgtgatcgcg aagcaccgcg cgctcgaggc gaagaagccg tacaagctcg agtactacgt gccgaagggc ggcgcgcccg tatggttcga cgtgatggcg atcccgaagg acgcgaagaa caaggacgcc gcgcttcaat ggatcaacta catcgaggat ccgaaggttc acgcgtcgat cacgaacgcg gtctactacc cgagcgcgaa cgcgcaagcg cgcaagtacg tgcgcccgga tgtcgcgaac gacccggccg tgtacccgcc tccggacgtc gtgaagacgc tgttcctgct caagccgctg ccgcccgaga tccagcgcct gcagacgcgt ctttggaccg agctgaaatc gggacgctaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa

Details for BupsA.00089.a.A2.PW30772

PURIFICATION DATe:	2/5/2011
CONCENTRATION:	29.06mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	12
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCGAAGGAT ACGCAGCTCA ACGTCTATAA CTGGTCGGAC TACATTGCGA AGGACACGAT CCCGAACTTC GAGAAGCAGA CGGGCGTCAA GGTCCGCTAC GACAACTACG ACAGCGACGA CACGCTGCAG GCGAAGCTTT TGACCGGCAA CTCGGGCTAC GACATCGTCG TGCCGACGAG CAACTACGCG GGCAAGCAGA TCCAGGCCGG CATCTTCACG CCGCTCGACA AGTCGAAGCT GCCGAACCTC AAGTATCTCG ATGCGCAACT GATGGCGCTC GTCGCGGGCG CCGATCCGGG CAACAAGTAC GTGGTGCCCT GGGCGTATGG CACGACGGGC CTCGGCTACA ACGTCGACAA GGCGCAGAAG GTGCTCGGCA AGGTGCCGCT CGACAACTGG GACATCCTGT TCAAGCCGGA GAACCTCTCG AAGCTGAAGA CGTGCGGCGT GTCGGTGCTC GACGCGCCGG ACCAGATGTT CGCGGCGACG CTGCACTACA TCGGCAAGGA TCCGATGAGC ACGAACCCGG CCGACTACCA GGCCGCGATG CAGGTGCTCA AGAAGATCCG CCCGTACATC ACGCAGTTCA ACTCGTCGGG CTACATCAAC GACATGGTCG GCGGCGACAT CTGCTTCGCG TTCGGCTGGT CGGGCGACGT CGTGATCGCG AAGCACCGCG CGCTCGAGGC GAAGAAGCCG TACAAGCTCG AGTACTACGT GCCGAAGGGC GGCGCGCCCG TATGGTTCGA CGTGATGGCG ATCCCGAAGG ACGCGAAGAA CAAGGACGCC GCGCTTCAAT GGATCAACTA CATCGAGGAT CCGAAGGTTC ACGCGTCGAT CACGAACGCG GTCTACTACC CGAGCGCGAA CGCGCAAGCG CGCAAGTACG TGCGCCCGGA TGTCGCGAAC GACCCGGCCG TGTACCCGCC TCCGGACGTC GTGAAGACGC TGTTCCTGCT CAAGCCGCTG CCGCCCGAGA TCCAGCGCCT GCAGACGCGT CTTTGGACCG AGCTGAAATC GGGACGCTAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA