BupsA.00088.a

ornithine carbamoyltransferase

CENTER ID: BupsA.00088.a
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: diffraction
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.00088.a.A1.GE25051 Full length( BupsA.00088.a ) 1 336
BupsA.00088.a.B1.GE25147 Full length( BupsA.00088.a ) 1 336
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.00088.a.B1.PS00081 Full length( BupsA.00088.a ) 1 336
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_127715
PATRIC ID: fig|320372.6.peg.2210
RefSeq: YP_333522
UniProt: Q3JSD1
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSFNLHNRNL LSLIHHNARE LRYLLDLARD LKRAKYSGTE QPRLLRKNIA LIFEKTSTRT RCAFEVAAYD QGANVTYIDP ASSQIGHKES MRDTARVLGR MYDAIEYRGF SQEIVEELAH HAGVPVFNGL TDEYHPTQML ADVMTMREHS DKPLHDIRYA YLGDARNNMG NSLLLIGAKL GMDVRIGAPK SLWPAADFIA QCEAFAAESG ARLTLTEDPY EAVKGVDFIH TDVWVSMGEP VEAWDERINA LLPYQVNRKL IESTGNPRTR FMHCLPSFHN CETKVGKQIA ERYPHLKDGI EVTDEVFESP RCIAFEQAEN RMHTIKAVLV STLGGI
NT Sequence
ATGAGCTTCA ACCTGCACAA CCGCAACCTG CTGAGCCTGA TCCACCACAA CGCGCGCGAG CTGCGCTATC TGCTCGACCT CGCGCGGGAT CTCAAGCGCG CGAAGTACAG CGGCACCGAG CAGCCGCGCC TGCTGCGCAA GAACATCGCG CTCATCTTCG AGAAGACCTC GACCCGCACG CGCTGCGCGT TCGAAGTCGC CGCATACGAT CAGGGCGCGA ACGTCACGTA CATCGATCCG GCTTCGTCGC AGATCGGCCA CAAGGAAAGC ATGAGGGACA CGGCGCGCGT GCTCGGGCGG ATGTACGACG CGATCGAGTA CCGTGGCTTC AGCCAGGAGA TCGTCGAGGA GCTCGCGCAC CACGCGGGCG TGCCCGTCTT CAACGGGCTC ACCGACGAAT ACCATCCGAC GCAGATGCTC GCCGACGTGA TGACGATGCG CGAGCACAGC GACAAGCCGC TGCACGACAT CCGCTACGCC TATCTCGGCG ACGCGCGCAA CAACATGGGC AATTCGCTGC TCCTGATCGG CGCGAAGCTC GGGATGGACG TGCGGATCGG CGCGCCGAAG TCGCTCTGGC CCGCGGCCGA CTTCATCGCG CAGTGCGAGG CGTTCGCGGC CGAGAGCGGC GCGCGGCTCA CGCTGACCGA GGACCCGTAC GAAGCGGTCA AGGGCGTCGA CTTCATCCAC ACCGACGTAT GGGTGTCGAT GGGCGAGCCC GTCGAGGCCT GGGACGAGCG GATCAACGCG CTCCTGCCGT ATCAGGTGAA CCGCAAGCTG ATCGAATCGA CGGGCAATCC GCGCACGCGC TTCATGCACT GCCTGCCGTC GTTCCACAAC TGCGAGACGA AGGTCGGCAA GCAGATCGCC GAGCGCTATC CGCACCTGAA GGACGGTATC GAAGTCACCG ACGAAGTGTT CGAATCGCCG CGCTGCATCG CGTTCGAGCA GGCGGAAAAC CGCATGCACA CGATCAAGGC GGTCCTCGTG TCGACGCTCG GCGGCATC
Details for BupsA.00088.a.A1.GE25051
HARVESTED ON: 2/12/2008
SEQUENCED ON: 8/15/2008
EXPECTED MW: 40kDa
OBSERVED MW: 41kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSFNLHNRN LLSLIHHNAR ELRYLLDLAR DLKRAKYSGT EQPRLLRKNI ALIFEKTSTR TRCAFEVAAY DQGANVTYID PASSQIGHKE SMRDTARVLG RMYDAIEYRG FSQEIVEELA HHAGVPVFNG LTDEYHPTQM LADVMTMREH SDKPLHDIRY AYLGDARNNM GNSLLLIGAK LGMDVRIGAP KSLWPAADFI AQCEAFAAES GARLTLTEDP YEAVKGVDFI HTGVWVSMGE PVEAWDERIN ALLPYQVNRK LIESTGNPRT RFMHCLPSFH NCETKVGKQI AERYPHLKDG IEVTDEVFES PRCIAFEQAE NRMYTIKAVL VSTLGGI
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgagct tcaacctgca caaccgcaac ctgctgagcc tgatccacca caacgcgcgc gagctgcgct atctgctcga cctcgcgcgg gatctcaagc gcgcgaagta cagcggcacc gagcagccgc gcctgctgcg caagaacatc gcgctcatct tcgagaagac ctcgacccgc acgcgctgcg cgttcgaagt cgccgcatac gatcagggcg cgaacgtcac gtacatcgat ccggcttcgt cgcagatcgg ccacaaggaa agcatgaggg acacggcgcg cgtgctcggg cggatgtacg acgcgatcga gtaccgtggc ttcagccagg agatcgtcga ggagctcgcg caccacgcgg gcgtgcccgt cttcaacggg ctcaccgacg aataccatcc gacgcagatg ctcgccgacg tgatgacgat gcgcgagcac agcgacaagc cgctgcacga catccgctac gcctatctcg gcgacgcgcg caacaacatg ggcaattcgc tgctcctgat cggcgcgaag ctcgggatgg acgtgcggat cggcgcgccg aagtcgctct ggcccgcggc cgacttcatc gcgcagtgcg aggcgttcgc ggccgagagc ggcgcgcggc tcacgctgac cgaggacccg tacgaagcgg tcaagggcgt cgacttcatc cacaccggcg tatgggtgtc gatgggcgag cccgtcgagg cctgggacga gcggatcaac gcgctcctgc cgtatcaggt gaaccgcaag ctgatcgaat cgacgggcaa tccgcgcacg cgcttcatgc actgcctgcc gtcgttccac aactgcgaga cgaaggtcgg caagcagatc gccgagcgct atccgcacct gaaggacggt atcgaagtca ccgacgaagt gttcgaatcg ccgcgctgca tcgcgttcga gcaggcggaa aaccgcatgt acacgatcaa ggcggtcctc gtgtcgacgc tcggcggcat ctaataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSFNLHNRN LLSLIHHNAR ELRYLLDLAR DLKRAKYSGT EQPRLLRKNI ALIFEKTSTR TRCAFEVAAY DQGANVTYID PASSQIGHKE SMRDTARVLG RMYDAIEYRG FSQEIVEELA HHAGVPVFNG LTDEYHPTQM LADVMTMREH SDKPLHDIRY AYLGDARNNM GNSLLLIGAK LGMDVRIGAP KSLWPAADFI AQCEAFAAES GARLTLTEDP YEAVKGVDFI HTDVWVSMGE PVEAWDERIN ALLPYQVNRK LIESTGNPRT RFMHCLPSFH NCETKVGKQI AERYPHLKDG IEVTDEVFES PRCIAFEQAE NRMHTIKAVL VSTLGGI
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcttcaac ctgcacaacc gcaacctgct gagcctgatc caccacaacg cgcgcgagct gcgctatctg ctcgacctcg cgcgggatct caagcgcgcg aagtacagcg gcaccgagca gccgcgcctg ctgcgcaaga acatcgcgct catcttcgag aagacctcga cccgcacgcg ctgcgcgttc gaagtcgccg catacgatca gggcgcgaac gtcacgtaca tcgatccggc ttcgtcgcag atcggccaca aggaaagcat gagggacacg gcgcgcgtgc tcgggcggat gtacgacgcg atcgagtacc gtggcttcag ccaggagatc gtcgaggagc tcgcgcacca cgcgggcgtg cccgtcttca acgggctcac cgacgaatac catccgacgc agatgctcgc cgacgtgatg acgatgcgcg agcacagcga caagccgctg cacgacatcc gctacgccta tctcggcgac gcgcgcaaca acatgggcaa ttcgctgctc ctgatcggcg cgaagctcgg gatggacgtg cggatcggcg cgccgaagtc gctctggccc gcggccgact tcatcgcgca gtgcgaggcg ttcgcggccg agagcggcgc gcggctcacg ctgaccgagg acccgtacga agcggtcaag ggcgtcgact tcatccacac cgacgtatgg gtgtcgatgg gcgagcccgt cgaggcctgg gacgagcgga tcaacgcgct cctgccgtat caggtgaacc gcaagctgat cgaatcgacg ggcaatccgc gcacgcgctt catgcactgc ctgccgtcgt tccacaactg cgagacgaag gtcggcaagc agatcgccga gcgctatccg cacctgaagg acggtatcga agtcaccgac gaagtgttcg aatcgccgcg ctgcatcgcg ttcgagcagg cggaaaaccg catgcacacg atcaaggcgg tcctcgtgtc gacgctcggc ggcatctgaa aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a
Details for BupsA.00088.a.B1.GE25147
HARVESTED ON: 2/26/2008
SEQUENCED ON: 3/16/2012
EXPECTED MW: 39kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMS FNLHNRNLLS LIHHNARELR YLLDLARDLK RAKYSGTEQP RLLRKNIALI FEKTSTRTRC AFEVAAYDQG ANVTYIDPAS SQIGHKESMR DTARVLGRMY DAIEYRGFSQ EIVEELAHHA GVPVFNGLTD EYHPTQMLAD VMTMREHSDK PLHDIRYAYL GDARNNMGNS LLLIGAKLGM DVRIGAPKSL WPAADFIAQC EAFAAESGAR LTLTEDPYEA VKGVDFIHTD VWVSMGEPVE AWDERINALL PYQVNRKLIE STGNPRTRFM HCLPSFHNCE TKVGKQIAER YPHLKDGIEV TDEVFESPRC IAFEQAENRM HTIKAVLVST LGGI
Validated NT Sequence
atggctcacc accaccacca ccatatgagc ttcaacctgc acaaccgcaa cctgctgagc ctgatccacc acaacgcgcg cgagctgcgc tatctgctcg acctcgcgcg ggatctcaag cgcgcgaagt acagcggcac cgagcagccg cgcctgctgc gcaagaacat cgcgctcatc ttcgagaaga cctcgacccg cacgcgctgc gcgttcgaag tcgccgcata cgatcagggc gcgaacgtca cgtacatcga tccggcttcg tcgcagatcg gccacaagga aagcatgagg gacacggcgc gcgtgctcgg gcggatgtac gacgcgatcg agtaccgtgg cttcagccag gagatcgtcg aggagctcgc gcaccacgcg ggcgtgcccg tcttcaacgg gctcaccgac gaataccatc cgacgcagat gctcgccgac gtgatgacga tgcgcgagca cagcgacaag ccgctgcacg acatccgcta cgcctatctc ggcgacgcgc gcaacaacat gggcaattcg ctgctcctga tcggcgcgaa gctcgggatg gacgtgcgga tcggcgcgcc gaagtcgctc tggcccgcgg ccgacttcat cgcgcagtgc gaggcgttcg cggccgagag cggcgcgcgg ctcacgctga ccgaggaccc gtacgaagcg gtcaagggcg tcgacttcat ccacaccgac gtatgggtgt cgatgggcga gcccgtcgag gcctgggacg agcggatcaa cgcgctcctg ccgtatcagg tgaaccgcaa gctgatcgaa tcgacgggca atccgcgcac gcgcttcatg cactgcctgc cgtcgttcca caactgcgag acgaaggtcg gcaagcagat cgccgagcgc tatccgcacc tgaaggacgg tatcgaagtc accgacgaag tgttcgaatc gccgcgctgc atcgcgttcg agcaggcgga aaaccgcatg cacacgatca aggcggtcct cgtgtcgacg ctcggcggca tctaagtgag taagatagga nccggctgct aaca
Expected Protein Sequence
MAHHHHHHMS FNLHNRNLLS LIHHNARELR YLLDLARDLK RAKYSGTEQP RLLRKNIALI FEKTSTRTRC AFEVAAYDQG ANVTYIDPAS SQIGHKESMR DTARVLGRMY DAIEYRGFSQ EIVEELAHHA GVPVFNGLTD EYHPTQMLAD VMTMREHSDK PLHDIRYAYL GDARNNMGNS LLLIGAKLGM DVRIGAPKSL WPAADFIAQC EAFAAESGAR LTLTEDPYEA VKGVDFIHTD VWVSMGEPVE AWDERINALL PYQVNRKLIE STGNPRTRFM HCLPSFHNCE TKVGKQIAER YPHLKDGIEV TDEVFESPRC IAFEQAENRM HTIKAVLVST LGGI
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagct tcaacctgca caaccgcaac ctgctgagcc tgatccacca caacgcgcgc gagctgcgct atctgctcga cctcgcgcgg gatctcaagc gcgcgaagta cagcggcacc gagcagccgc gcctgctgcg caagaacatc gcgctcatct tcgagaagac ctcgacccgc acgcgctgcg cgttcgaagt cgccgcatac gatcagggcg cgaacgtcac gtacatcgat ccggcttcgt cgcagatcgg ccacaaggaa agcatgaggg acacggcgcg cgtgctcggg cggatgtacg acgcgatcga gtaccgtggc ttcagccagg agatcgtcga ggagctcgcg caccacgcgg gcgtgcccgt cttcaacggg ctcaccgacg aataccatcc gacgcagatg ctcgccgacg tgatgacgat gcgcgagcac agcgacaagc cgctgcacga catccgctac gcctatctcg gcgacgcgcg caacaacatg ggcaattcgc tgctcctgat cggcgcgaag ctcgggatgg acgtgcggat cggcgcgccg aagtcgctct ggcccgcggc cgacttcatc gcgcagtgcg aggcgttcgc ggccgagagc ggcgcgcggc tcacgctgac cgaggacccg tacgaagcgg tcaagggcgt cgacttcatc cacaccgacg tatgggtgtc gatgggcgag cccgtcgagg cctgggacga gcggatcaac gcgctcctgc cgtatcaggt gaaccgcaag ctgatcgaat cgacgggcaa tccgcgcacg cgcttcatgc actgcctgcc gtcgttccac aactgcgaga cgaaggtcgg caagcagatc gccgagcgct atccgcacct gaaggacggt atcgaagtca ccgacgaagt gttcgaatcg ccgcgctgca tcgcgttcga gcaggcggaa aaccgcatgc acacgatcaa ggcggtcctc gtgtcgacgc tcggcggcat ctgatgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for BupsA.00088.a.B1.PS00081
PURIFICATION DATe: 9/24/2008
CONCENTRATION: 15.5mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 2
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS FNLHNRNLLS LIHHNARELR YLLDLARDLK RAKYSGTEQP RLLRKNIALI FEKTSTRTRC AFEVAAYDQG ANVTYIDPAS SQIGHKESMR DTARVLGRMY DAIEYRGFSQ EIVEELAHHA GVPVFNGLTD EYHPTQMLAD VMTMREHSDK PLHDIRYAYL GDARNNMGNS LLLIGAKLGM DVRIGAPKSL WPAADFIAQC EAFAAESGAR LTLTEDPYEA VKGVDFIHTD VWVSMGEPVE AWDERINALL PYQVNRKLIE STGNPRTRFM HCLPSFHNCE TKVGKQIAER YPHLKDGIEV TDEVFESPRC IAFEQAENRM HTIKAVLVST LGGI
Validated NT Sequence
atggctcacc accaccacca ccatatgagc ttcaacctgc acaaccgcaa cctgctgagc ctgatccacc acaacgcgcg cgagctgcgc tatctgctcg acctcgcgcg ggatctcaag cgcgcgaagt acagcggcac cgagcagccg cgcctgctgc gcaagaacat cgcgctcatc ttcgagaaga cctcgacccg cacgcgctgc gcgttcgaag tcgccgcata cgatcagggc gcgaacgtca cgtacatcga tccggcttcg tcgcagatcg gccacaagga aagcatgagg gacacggcgc gcgtgctcgg gcggatgtac gacgcgatcg agtaccgtgg cttcagccag gagatcgtcg aggagctcgc gcaccacgcg ggcgtgcccg tcttcaacgg gctcaccgac gaataccatc cgacgcagat gctcgccgac gtgatgacga tgcgcgagca cagcgacaag ccgctgcacg acatccgcta cgcctatctc ggcgacgcgc gcaacaacat gggcaattcg ctgctcctga tcggcgcgaa gctcgggatg gacgtgcgga tcggcgcgcc gaagtcgctc tggcccgcgg ccgacttcat cgcgcagtgc gaggcgttcg cggccgagag cggcgcgcgg ctcacgctga ccgaggaccc gtacgaagcg gtcaagggcg tcgacttcat ccacaccgac gtatgggtgt cgatgggcga gcccgtcgag gcctgggacg agcggatcaa cgcgctcctg ccgtatcagg tgaaccgcaa gctgatcgaa tcgacgggca atccgcgcac gcgcttcatg cactgcctgc cgtcgttcca caactgcgag acgaaggtcg gcaagcagat cgccgagcgc tatccgcacc tgaaggacgg tatcgaagtc accgacgaag tgttcgaatc gccgcgctgc atcgcgttcg agcaggcgga aaaccgcatg cacacgatca aggcggtcct cgtgtcgacg ctcggcggca tctaagtgag taagatagga nccggctgct aaca
Expressed Protein Sequence
MAHHHHHHMS FNLHNRNLLS LIHHNARELR YLLDLARDLK RAKYSGTEQP RLLRKNIALI FEKTSTRTRC AFEVAAYDQG ANVTYIDPAS SQIGHKESMR DTARVLGRMY DAIEYRGFSQ EIVEELAHHA GVPVFNGLTD EYHPTQMLAD VMTMREHSDK PLHDIRYAYL GDARNNMGNS LLLIGAKLGM DVRIGAPKSL WPAADFIAQC EAFAAESGAR LTLTEDPYEA VKGVDFIHTD VWVSMGEPVE AWDERINALL PYQVNRKLIE STGNPRTRFM HCLPSFHNCE TKVGKQIAER YPHLKDGIEV TDEVFESPRC IAFEQAENRM HTIKAVLVST LGGI
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGCT TCAACCTGCA CAACCGCAAC CTGCTGAGCC TGATCCACCA CAACGCGCGC GAGCTGCGCT ATCTGCTCGA CCTCGCGCGG GATCTCAAGC GCGCGAAGTA CAGCGGCACC GAGCAGCCGC GCCTGCTGCG CAAGAACATC GCGCTCATCT TCGAGAAGAC CTCGACCCGC ACGCGCTGCG CGTTCGAAGT CGCCGCATAC GATCAGGGCG CGAACGTCAC GTACATCGAT CCGGCTTCGT CGCAGATCGG CCACAAGGAA AGCATGAGGG ACACGGCGCG CGTGCTCGGG CGGATGTACG ACGCGATCGA GTACCGTGGC TTCAGCCAGG AGATCGTCGA GGAGCTCGCG CACCACGCGG GCGTGCCCGT CTTCAACGGG CTCACCGACG AATACCATCC GACGCAGATG CTCGCCGACG TGATGACGAT GCGCGAGCAC AGCGACAAGC CGCTGCACGA CATCCGCTAC GCCTATCTCG GCGACGCGCG CAACAACATG GGCAATTCGC TGCTCCTGAT CGGCGCGAAG CTCGGGATGG ACGTGCGGAT CGGCGCGCCG AAGTCGCTCT GGCCCGCGGC CGACTTCATC GCGCAGTGCG AGGCGTTCGC GGCCGAGAGC GGCGCGCGGC TCACGCTGAC CGAGGACCCG TACGAAGCGG TCAAGGGCGT CGACTTCATC CACACCGACG TATGGGTGTC GATGGGCGAG CCCGTCGAGG CCTGGGACGA GCGGATCAAC GCGCTCCTGC CGTATCAGGT GAACCGCAAG CTGATCGAAT CGACGGGCAA TCCGCGCACG CGCTTCATGC ACTGCCTGCC GTCGTTCCAC AACTGCGAGA CGAAGGTCGG CAAGCAGATC GCCGAGCGCT ATCCGCACCT GAAGGACGGT ATCGAAGTCA CCGACGAAGT GTTCGAATCG CCGCGCTGCA TCGCGTTCGA GCAGGCGGAA AACCGCATGC ACACGATCAA GGCGGTCCTC GTGTCGACGC TCGGCGGCAT CTGATGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT