BupsA.00085.b
UDP-glucose 4-epimerase
| CENTER ID: | BupsA.00085.b |
| ORGANISM: | Burkholderia pseudomallei 1710b |
| ASSOCIATED DISEASE: | Melioidosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BupsA.00085.b.A1.GE25048 | Full length( BupsA.00085.b ) | 1 | 340 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BupsA.00085.b.B1.PS00019 | Full length( BupsA.00085.b ) | 1 | 340 | ||
| BupsA.00085.b.B1.PS00025 | Full length( BupsA.00085.b ) | 1 | 340 |
Structures
3ENK
DEPOSITED: 9/25/2008
DETERMINATION: XRay
CLONE: BupsA.00085.b.B1.GE25144
PROTEIN: BupsA.00085.b.B1.PS00025
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_126853 |
| PATRIC ID: | fig|320372.6.peg.3282 |
| RefSeq: | YP_334520 |
| UniProt: | Q3JPI3 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSTKGTILVT GGAGYIGSHT AVELLAHGYD VVIADNLVNS KREAIARIEK ITGKTPAFHE TDVSDERALA RIFDAHPITA AIHFAALKAV GESVAKPIEY YRNNLDSLLS LLRVMRERAV KRIVFSSSAT VYGVPERSPI DETFPLSATN PYGQTKLMAE QILRDVEAAD PSWRVATLRY FNPVGAHESG LIGEDPAGIP NNLMPYVAQV AVGKLEKLRV FGSDYPTPDG TGVRDYIHVV DLARGHIAAL DALERRDASL TVNLGTGRGY SVLEVVRAFE KASGRAVPYE LVARRPGDVA ECYANPAAAA ETIGWKAERD LERMCADHWR WQENNPRGFV
NT Sequence
ATGAGCACGA AGGGCACGAT CCTCGTCACC GGCGGCGCGG GCTATATCGG TTCGCACACC GCCGTCGAGC TGCTCGCGCA CGGCTACGAC GTCGTGATCG CCGACAACCT CGTCAACAGC AAGCGCGAGG CGATCGCGCG GATCGAGAAG ATCACCGGCA AGACGCCGGC GTTTCACGAA ACCGACGTGA GCGACGAGCG CGCGCTCGCG CGGATCTTCG ACGCGCATCC GATCACGGCC GCGATCCATT TCGCGGCGCT GAAGGCGGTC GGCGAATCGG TCGCGAAGCC CATCGAGTAC TACCGCAACA ACCTCGACAG CCTGCTGTCG CTGCTGCGCG TGATGCGCGA GCGCGCCGTG AAGCGGATCG TGTTCAGCTC GTCGGCGACC GTATACGGCG TACCCGAGCG CTCGCCGATC GACGAGACGT TCCCGCTTTC GGCGACCAAC CCGTACGGCC AGACGAAGCT GATGGCCGAG CAGATCCTGC GCGATGTCGA AGCCGCCGAT CCGTCCTGGC GCGTCGCGAC GCTGCGCTAC TTCAATCCGG TCGGCGCGCA CGAAAGCGGC CTCATCGGCG AGGACCCGGC CGGCATTCCG AACAACCTGA TGCCGTACGT CGCGCAGGTC GCGGTGGGCA AGCTCGAGAA GCTGCGCGTG TTCGGCAGCG ACTACCCGAC GCCCGACGGC ACCGGCGTGC GCGATTACAT CCACGTCGTC GATCTCGCGC GCGGCCACAT CGCGGCGCTC GACGCGCTCG AGCGCCGCGA CGCGAGCCTC ACCGTCAACC TCGGCACGGG CCGCGGCTAC AGCGTGCTCG AAGTCGTGCG CGCGTTCGAG AAGGCGTCGG GGCGCGCCGT GCCGTACGAA CTCGTCGCGC GCCGCCCGGG CGACGTCGCC GAGTGCTACG CGAACCCCGC CGCGGCGGCC GAGACGATCG GATGGAAGGC CGAGCGCGAC CTCGAGCGGA TGTGCGCGGA CCACTGGCGC TGGCAGGAGA ACAACCCGCG CGGTTTTGTA
Details for BupsA.00085.b.A1.GE25048
| HARVESTED ON: | 3/31/2008 |
| SEQUENCED ON: | 7/28/2010 |
| EXPECTED MW: | 39kDa |
| OBSERVED MW: | 39kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 69 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSTKGTILV TGGAGYIGSH TAVELLAHGY DVVIADNLVN SKREAIARIE KITGKTPAFH ETDVSDERAL ARIFDAHPIT AAIHFAALKA VGESVAKPIE YYRNNLDSLL SLLRVMRERA VKRIVFSSSA TVYGVPERSP IDETFPLSAT NPYGQTKLMA EQILRDVEAA DPSWRVATLR YFNPVGAHES GLIGEDPXGI PNNLMPYVAX VAVGKLEKLR VFGSDYPTPD
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgagca cgaagggcac gatcctcgtc accggcggcg cgggctatat cggttcgcac accgccgtcg agctgctcgc gcacggctac gacgtcgtga tcgccgacaa cctcgtcaac agcaagcgcg aggcgatcgc gcggatcgag aagatcaccg gcaagacgcc ggcgtttcac gaaaccgacg tgagcgacga gcgcgcgctc gcgcggatct tcgacgcgca tccgatcacg gccgcgatcc atttcgcggc gctgaaggcg gtcggcgaat cggtcgcgaa gcccatcgag tactaccgca acaacctcga cagcctgctg tcgctgctgc gcgtgatgcg cgagcgcgcc gtgaagcgga tcgtgttcag ctcgtcggcg accgtatacg gcgtacccga gcgctcgccg atcgacgaga cgttcccgct ttcggcgacc aacccgtacg gccagacgaa gctgatggcc gagcagatcc tgcgcgatgt cgaagccgcc gatccgtcct ggcgcgtcgc gacgctgcgc tacttcaatc cggtcggcgc gcacgaaagc ggcctcatcg gcgaggaccc ggncggcatt ccgaacaacc tgatgccgta cgtcgcgcan gtcgcggtgg gcaagctcga gaagctgcgc gtgttcggca gcgactaccc gacgcccgac ggc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSTKGTILV TGGAGYIGSH TAVELLAHGY DVVIADNLVN SKREAIARIE KITGKTPAFH ETDVSDERAL ARIFDAHPIT AAIHFAALKA VGESVAKPIE YYRNNLDSLL SLLRVMRERA VKRIVFSSSA TVYGVPERSP IDETFPLSAT NPYGQTKLMA EQILRDVEAA DPSWRVATLR YFNPVGAHES GLIGEDPAGI PNNLMPYVAQ VAVGKLEKLR VFGSDYPTPD GTGVRDYIHV VDLARGHIAA LDALERRDAS LTVNLGTGRG YSVLEVVRAF EKASGRAVPY ELVARRPGDV AECYANPAAA AETIGWKAER DLERMCADHW RWQENNPRGF V
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcacgaag ggcacgatcc tcgtcaccgg cggcgcgggc tatatcggtt cgcacaccgc cgtcgagctg ctcgcgcacg gctacgacgt cgtgatcgcc gacaacctcg tcaacagcaa gcgcgaggcg atcgcgcgga tcgagaagat caccggcaag acgccggcgt ttcacgaaac cgacgtgagc gacgagcgcg cgctcgcgcg gatcttcgac gcgcatccga tcacggccgc gatccatttc gcggcgctga aggcggtcgg cgaatcggtc gcgaagccca tcgagtacta ccgcaacaac ctcgacagcc tgctgtcgct gctgcgcgtg atgcgcgagc gcgccgtgaa gcggatcgtg ttcagctcgt cggcgaccgt atacggcgta cccgagcgct cgccgatcga cgagacgttc ccgctttcgg cgaccaaccc gtacggccag acgaagctga tggccgagca gatcctgcgc gatgtcgaag ccgccgatcc gtcctggcgc gtcgcgacgc tgcgctactt caatccggtc ggcgcgcacg aaagcggcct catcggcgag gacccggccg gcattccgaa caacctgatg ccgtacgtcg cgcaggtcgc ggtgggcaag ctcgagaagc tgcgcgtgtt cggcagcgac tacccgacgc ccgacggcac cggcgtgcgc gattacatcc acgtcgtcga tctcgcgcgc ggccacatcg cggcgctcga cgcgctcgag cgccgcgacg cgagcctcac cgtcaacctc ggcacgggcc gcggctacag cgtgctcgaa gtcgtgcgcg cgttcgagaa ggcgtcgggg cgcgccgtgc cgtacgaact cgtcgcgcgc cgcccgggcg acgtcgccga gtgctacgcg aaccccgccg cggcggccga gacgatcgga tggaaggccg agcgcgacct cgagcggatg tgcgcggacc actggcgctg gcaggagaac aacccgcgcg gttttgtata aaaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa
Details for BupsA.00085.b.B1.PS00019
| PURIFICATION DATe: | 5/29/2008 |
| CONCENTRATION: | 14.89mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 2 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS TKGTILVTGG AGYIGSHTAV ELLAHGYDVV IADNLVNSKR EAIARIEKIT GKTPAFHETD VSDERALARI FDAHPITAAI HFAALKAVGE SVAKPIEYYR NNLDSLLSLL RVMRERAVKR IVFSSSATVY GVPERSPIDE TFPLSATNPY GQTKLMAEQI LRDVEAADPS WRVATLRYFN PVGAHESGLI GEDPAGIPNN LMPYVAQVAV GKLEKLRVFG SDYPTPDGTG VRDYIHVVDL ARGHIAALDA LERRDASLTV NLGTGRGYSV LEVVRAFEKA SGRAVPYELV ARRPGDVAEC YANPAAAAET IGWKAERDLE RMCADHWRWQ ENNPRGFV
Validated NT Sequence
tttangaagg aganatacca tggctcacca ccaccaccac catatgagca cgaagggcac gatcctcgtc accggcggcg cgggctatat cggttcgcac accgccgtcg agctgctcgc gcacggctac gacgtcgtga tcgccgacaa cctcgtcaac agcaagcgcg aggcgatcgc gcggatcgag aagatcaccg gcaagacgcc ggcgtttcac gaaaccgacg tgagcgacga gcgcgcgctc gcgcggatct tcgacgcgca tccgatcacg gccgcgatcc atttcgcggc gctgaaggcg gtcggcgaat cggtcgcgaa gcccatcgag tactaccgca acaacctcga cagcctgctg tcgctgctgc gcgtgatgcg cgagcgcgcc gtgaagcgga tcgtgttcag ctcgtcggcg accgtatacg gcgtacccga gcgctcgccg atcgacgaga cgttcccgct ttcggcgacc aacccgtacg gccagacgaa gctgatggcc gagcagatcc tgcgcgatgt cgaagccgcc gatccgtcct ggcgcgtcgc gacgctgcgc tacttcaatc cggtcggcgc gcacgaaagc ggcctcatcg gcgaggaccc ggccggcatt ccgaacaacc tgatgccgta cgtcgcgcag gtcgcggtgg gcaagctcga gaagctgcgc gtgttcggca gcgactaccc gacgcccgac ggcaccggcg tgcgcgatta catccacgtc gtcgatctcg cgcgcggcca catcgcggcg ctcgacgcgc tcgagcgccg cgacgcgagc ctcaccgtca acctcggcac gggccgcggc tacagcgtgc tcgaagtcgt gcgcgcgttc gagaaggcgt cggggcgcgc cgtgccgtac gaactcgtcg cgcgccgccc gggcgacgtc gccgagtgct acgcgaaccc cgccgcggcg gccgagacga tcggatggaa ggccgagcgc gacctcgagc ggatgtgcgc ggaccactgg cgctggcagg agaacaaccc gcgcggtttt gtataagtga gt
Expressed Protein Sequence
MAHHHHHHMS TKGTILVTGG AGYIGSHTAV ELLAHGYDVV IADNLVNSKR EAIARIEKIT GKTPAFHETD VSDERALARI FDAHPITAAI HFAALKAVGE SVAKPIEYYR NNLDSLLSLL RVMRERAVKR IVFSSSATVY GVPERSPIDE TFPLSATNPY GQTKLMAEQI LRDVEAADPS WRVATLRYFN PVGAHESGLI GEDPAGIPNN LMPYVAQVAV GKLEKLRVFG SDYPTPDGTG VRDYIHVVDL ARGHIAALDA LERRDASLTV NLGTGRGYSV LEVVRAFEKA SGRAVPYELV ARRPGDVAEC YANPAAAAET IGWKAERDLE RMCADHWRWQ ENNPRGFV
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGCA CGAAGGGCAC GATCCTCGTC ACCGGCGGCG CGGGCTATAT CGGTTCGCAC ACCGCCGTCG AGCTGCTCGC GCACGGCTAC GACGTCGTGA TCGCCGACAA CCTCGTCAAC AGCAAGCGCG AGGCGATCGC GCGGATCGAG AAGATCACCG GCAAGACGCC GGCGTTTCAC GAAACCGACG TGAGCGACGA GCGCGCGCTC GCGCGGATCT TCGACGCGCA TCCGATCACG GCCGCGATCC ATTTCGCGGC GCTGAAGGCG GTCGGCGAAT CGGTCGCGAA GCCCATCGAG TACTACCGCA ACAACCTCGA CAGCCTGCTG TCGCTGCTGC GCGTGATGCG CGAGCGCGCC GTGAAGCGGA TCGTGTTCAG CTCGTCGGCG ACCGTATACG GCGTACCCGA GCGCTCGCCG ATCGACGAGA CGTTCCCGCT TTCGGCGACC AACCCGTACG GCCAGACGAA GCTGATGGCC GAGCAGATCC TGCGCGATGT CGAAGCCGCC GATCCGTCCT GGCGCGTCGC GACGCTGCGC TACTTCAATC CGGTCGGCGC GCACGAAAGC GGCCTCATCG GCGAGGACCC GGCCGGCATT CCGAACAACC TGATGCCGTA CGTCGCGCAG GTCGCGGTGG GCAAGCTCGA GAAGCTGCGC GTGTTCGGCA GCGACTACCC GACGCCCGAC GGCACCGGCG TGCGCGATTA CATCCACGTC GTCGATCTCG CGCGCGGCCA CATCGCGGCG CTCGACGCGC TCGAGCGCCG CGACGCGAGC CTCACCGTCA ACCTCGGCAC GGGCCGCGGC TACAGCGTGC TCGAAGTCGT GCGCGCGTTC GAGAAGGCGT CGGGGCGCGC CGTGCCGTAC GAACTCGTCG CGCGCCGCCC GGGCGACGTC GCCGAGTGCT ACGCGAACCC CGCCGCGGCG GCCGAGACGA TCGGATGGAA GGCCGAGCGC GACCTCGAGC GGATGTGCGC GGACCACTGG CGCTGGCAGG AGAACAACCC GCGCGGTTTT GTATAATGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T
Details for BupsA.00085.b.B1.PS00025
| PURIFICATION DATe: | 6/17/2008 |
| CONCENTRATION: | 9.42mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 11 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS TKGTILVTGG AGYIGSHTAV ELLAHGYDVV IADNLVNSKR EAIARIEKIT GKTPAFHETD VSDERALARI FDAHPITAAI HFAALKAVGE SVAKPIEYYR NNLDSLLSLL RVMRERAVKR IVFSSSATVY GVPERSPIDE TFPLSATNPY GQTKLMAEQI LRDVEAADPS WRVATLRYFN PVGAHESGLI GEDPAGIPNN LMPYVAQVAV GKLEKLRVFG SDYPTPDGTG VRDYIHVVDL ARGHIAALDA LERRDASLTV NLGTGRGYSV LEVVRAFEKA SGRAVPYELV ARRPGDVAEC YANPAAAAET IGWKAERDLE RMCADHWRWQ ENNPRGFV
Validated NT Sequence
tttangaagg aganatacca tggctcacca ccaccaccac catatgagca cgaagggcac gatcctcgtc accggcggcg cgggctatat cggttcgcac accgccgtcg agctgctcgc gcacggctac gacgtcgtga tcgccgacaa cctcgtcaac agcaagcgcg aggcgatcgc gcggatcgag aagatcaccg gcaagacgcc ggcgtttcac gaaaccgacg tgagcgacga gcgcgcgctc gcgcggatct tcgacgcgca tccgatcacg gccgcgatcc atttcgcggc gctgaaggcg gtcggcgaat cggtcgcgaa gcccatcgag tactaccgca acaacctcga cagcctgctg tcgctgctgc gcgtgatgcg cgagcgcgcc gtgaagcgga tcgtgttcag ctcgtcggcg accgtatacg gcgtacccga gcgctcgccg atcgacgaga cgttcccgct ttcggcgacc aacccgtacg gccagacgaa gctgatggcc gagcagatcc tgcgcgatgt cgaagccgcc gatccgtcct ggcgcgtcgc gacgctgcgc tacttcaatc cggtcggcgc gcacgaaagc ggcctcatcg gcgaggaccc ggccggcatt ccgaacaacc tgatgccgta cgtcgcgcag gtcgcggtgg gcaagctcga gaagctgcgc gtgttcggca gcgactaccc gacgcccgac ggcaccggcg tgcgcgatta catccacgtc gtcgatctcg cgcgcggcca catcgcggcg ctcgacgcgc tcgagcgccg cgacgcgagc ctcaccgtca acctcggcac gggccgcggc tacagcgtgc tcgaagtcgt gcgcgcgttc gagaaggcgt cggggcgcgc cgtgccgtac gaactcgtcg cgcgccgccc gggcgacgtc gccgagtgct acgcgaaccc cgccgcggcg gccgagacga tcggatggaa ggccgagcgc gacctcgagc ggatgtgcgc ggaccactgg cgctggcagg agaacaaccc gcgcggtttt gtataagtga gt
Expressed Protein Sequence
MAHHHHHHMS TKGTILVTGG AGYIGSHTAV ELLAHGYDVV IADNLVNSKR EAIARIEKIT GKTPAFHETD VSDERALARI FDAHPITAAI HFAALKAVGE SVAKPIEYYR NNLDSLLSLL RVMRERAVKR IVFSSSATVY GVPERSPIDE TFPLSATNPY GQTKLMAEQI LRDVEAADPS WRVATLRYFN PVGAHESGLI GEDPAGIPNN LMPYVAQVAV GKLEKLRVFG SDYPTPDGTG VRDYIHVVDL ARGHIAALDA LERRDASLTV NLGTGRGYSV LEVVRAFEKA SGRAVPYELV ARRPGDVAEC YANPAAAAET IGWKAERDLE RMCADHWRWQ ENNPRGFV
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGCA CGAAGGGCAC GATCCTCGTC ACCGGCGGCG CGGGCTATAT CGGTTCGCAC ACCGCCGTCG AGCTGCTCGC GCACGGCTAC GACGTCGTGA TCGCCGACAA CCTCGTCAAC AGCAAGCGCG AGGCGATCGC GCGGATCGAG AAGATCACCG GCAAGACGCC GGCGTTTCAC GAAACCGACG TGAGCGACGA GCGCGCGCTC GCGCGGATCT TCGACGCGCA TCCGATCACG GCCGCGATCC ATTTCGCGGC GCTGAAGGCG GTCGGCGAAT CGGTCGCGAA GCCCATCGAG TACTACCGCA ACAACCTCGA CAGCCTGCTG TCGCTGCTGC GCGTGATGCG CGAGCGCGCC GTGAAGCGGA TCGTGTTCAG CTCGTCGGCG ACCGTATACG GCGTACCCGA GCGCTCGCCG ATCGACGAGA CGTTCCCGCT TTCGGCGACC AACCCGTACG GCCAGACGAA GCTGATGGCC GAGCAGATCC TGCGCGATGT CGAAGCCGCC GATCCGTCCT GGCGCGTCGC GACGCTGCGC TACTTCAATC CGGTCGGCGC GCACGAAAGC GGCCTCATCG GCGAGGACCC GGCCGGCATT CCGAACAACC TGATGCCGTA CGTCGCGCAG GTCGCGGTGG GCAAGCTCGA GAAGCTGCGC GTGTTCGGCA GCGACTACCC GACGCCCGAC GGCACCGGCG TGCGCGATTA CATCCACGTC GTCGATCTCG CGCGCGGCCA CATCGCGGCG CTCGACGCGC TCGAGCGCCG CGACGCGAGC CTCACCGTCA ACCTCGGCAC GGGCCGCGGC TACAGCGTGC TCGAAGTCGT GCGCGCGTTC GAGAAGGCGT CGGGGCGCGC CGTGCCGTAC GAACTCGTCG CGCGCCGCCC GGGCGACGTC GCCGAGTGCT ACGCGAACCC CGCCGCGGCG GCCGAGACGA TCGGATGGAA GGCCGAGCGC GACCTCGAGC GGATGTGCGC GGACCACTGG CGCTGGCAGG AGAACAACCC GCGCGGTTTT GTATAATGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T