SSGCID: Target Page

BupsA.00085.a

dTDP-glucose 4,6-dehydratase

CENTER ID:	BupsA.00085.a
ORGANISM:	Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE:	Melioidosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.00085.a.A1.GE25047	Full length( BupsA.00085.a )		1	400

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|320372.6.peg.3298
RefSeq:	YP_334535
UniProt:	Q3JPG8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MGAWLRSSHA AATGRCHSSD QRHASERAGH AAHAGCLTDS QFQQRNPMIL VTGGAGFIGA NFVLDWLAQS DEAVLNVDKL TYAGNLGTLK SLQGNPKHVF ARVDICDRAA IDALLAQHKP RAIVHFAAES HVDRSIHGPA DFVQTNVVGT FTLLEAARQY WSALGTDAKA AFRFLHVSTD EVFGSLSPAD PQFSETTPYA PNSPYSATKA GSDHLVRAYH HTYGLPVLTT NCSNNYGPYQ FPEKLIPLMI ANALGGKPLP VYGDGQNVRD WLYVGDHCSA IREVLARGVP GETYNVGGWN EKKNLDVVHT LCDLLDEARP KAGGSYRDQI TYVTDRPGHD RRYAIDARKL ERELGWKPAE TFETGLAKTV RWYLDHQEWV DEVVSGDYRK WVETNYAQRA

NT Sequence

TTGGGCGCAT GGCTTCGATC GAGCCACGCG GCCGCAACCG GGCGGTGTCA TTCGAGCGAC CAACGGCATG CTTCCGAGCG TGCCGGCCAT GCTGCGCACG CCGGTTGTTT GACCGACTCC CAATTTCAGC AAAGGAATCC CATGATCTTG GTAACGGGCG GCGCCGGTTT CATCGGCGCC AACTTCGTGC TTGACTGGCT GGCTCAATCC GACGAGGCGG TGCTGAACGT CGATAAGCTG ACGTACGCGG GCAACCTCGG CACGTTGAAG TCGTTGCAGG GGAATCCGAA GCATGTATTC GCCCGCGTCG ACATCTGCGA TCGCGCCGCA ATCGACGCGC TGCTGGCGCA GCACAAGCCG CGCGCGATCG TGCACTTCGC GGCCGAGAGC CACGTCGACC GTTCGATTCA CGGTCCGGCC GATTTCGTGC AGACGAACGT CGTCGGCACC TTCACGCTGC TCGAAGCCGC GCGCCAATAC TGGAGCGCGC TCGGCACCGA CGCGAAGGCG GCGTTCCGCT TCCTGCACGT ATCGACGGAC GAAGTGTTCG GCTCGCTGTC GCCGGCCGAT CCGCAGTTCT CCGAAACCAC GCCGTACGCG CCGAACAGCC CGTACTCGGC GACGAAGGCG GGCTCCGACC ACCTCGTACG CGCGTATCAC CACACGTACG GGTTGCCGGT GCTCACGACG AACTGCTCGA ACAACTACGG CCCGTATCAG TTCCCCGAGA AGCTGATTCC GCTGATGATC GCGAACGCGC TCGGCGGCAA GCCGCTGCCC GTCTACGGCG ACGGCCAGAA CGTGCGGGAC TGGCTGTACG TCGGCGATCA CTGCAGCGCG ATTCGCGAGG TGCTCGCGCG CGGCGTGCCG GGCGAGACGT ACAACGTCGG CGGCTGGAAC GAAAAGAAGA ATCTCGATGT CGTGCACACG CTGTGCGATC TGCTCGACGA GGCCCGGCCG AAGGCGGGCG GCTCGTACCG CGATCAGATC ACGTACGTGA CCGATCGCCC CGGCCACGAT CGCCGCTATG CGATCGATGC GCGCAAGCTC GAGCGCGAGC TCGGCTGGAA GCCGGCCGAG ACGTTCGAGA CCGGACTTGC GAAGACCGTG CGCTGGTATC TCGACCATCA GGAGTGGGTC GACGAAGTGG TGTCGGGCGA TTACCGCAAG TGGGTCGAAA CGAACTACGC GCAACGCGCG

Details for BupsA.00085.a.A1.GE25047

HARVESTED ON:	2/12/2008
SEQUENCED ON:	6/16/2009
EXPECTED MW:	46kDa
OBSERVED MW:	47kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	98
PERCENT COVERAGE:	73

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMLGAWLRSS HAAATGRCHS SDQRHASERA GHAAHAGCLT DSQFQQRNPM ILVTGGAGFI GANFVLDWLA QSDEAVLNVD KLTYAGNLGT LKSLQGNPKH VFARVDICDR AAIDALLAQH KPRAIVHFAA ESHVDRSIHG PADFVQTNVV GTFTLLEAAR QYWSALGTDA KAAFRFLHVS TDEVFGSLSP ADPQFSETTP YAPNSPYSAT KAGSDHLVRA YHHTYGLPVL TTNCSNNYGP YQFPEKLXPL MIANALGGKP LPVYGXGQNV RDWLYVGXHC SAIREXXARG

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgttgg gcgcatggct tcgatcgagc cacgcggccg caaccgggcg gtgtcattcg agcgaccaac ggcatgcttc cgagcgtgcc ggccatgctg cgcacgccgg ttgtttgacc gactcccaat ttcagcaaag gaatcccatg atcttggtaa cgggcggcgc cggtttcatc ggcgccaact tcgtgcttga ctggctggct caatccgacg aggcggtgct gaacgtcgat aagctgacgt acgcgggcaa cctcggcacg ttgaagtcgt tgcaggggaa tccgaagcat gtattcgccc gcgtcgacat ctgcgatcgc gccgcaatcg acgcgctgct ggcgcagcac aagccgcgcg cgatcgtgca cttcgcggcc gagagccacg tcgaccgttc gattcacggt ccggccgatt tcgtgcagac gaacgtcgtc ggcaccttca cgctgctcga agccgcgcgc caatactgga gcgcgctcgg caccgacgcg aaggcggcgt tccgcttcct gcacgtatcg acggacgaag tgttcggctc gctgtcgccg gccgatccgc agttctccga aaccacgccg tacgcgccga acagcccgta ctcggcgacg aaggcgggct ccgaccacct cgtacgcgcg tatcaccaca cgtacgggtt gccggtgctc acgacgaact gctcgaacaa ctacggcccg tatcagttcc ccgagaagct gantccgctg atgatcgcga acgcgctcgg cggcaagccg ctgcccgtct acggcgangg ncagaacgtg cgggactggc tgtacgtcgg cnatcactgc agcgcgattc gcgaggnnnt cgcgcgcggc gtg

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttgggcgca tggcttcgat cgagccacgc ggccgcaacc gggcggtgtc attcgagcga ccaacggcat gcttccgagc gtgccggcca tgctgcgcac gccggttgtt tgaccgactc ccaatttcag caaaggaatc ccatgatctt ggtaacgggc ggcgccggtt tcatcggcgc caacttcgtg cttgactggc tggctcaatc cgacgaggcg gtgctgaacg tcgataagct gacgtacgcg ggcaacctcg gcacgttgaa gtcgttgcag gggaatccga agcatgtatt cgcccgcgtc gacatctgcg atcgcgccgc aatcgacgcg ctgctggcgc agcacaagcc gcgcgcgatc gtgcacttcg cggccgagag ccacgtcgac cgttcgattc acggtccggc cgatttcgtg cagacgaacg tcgtcggcac cttcacgctg ctcgaagccg cgcgccaata ctggagcgcg ctcggcaccg acgcgaaggc ggcgttccgc ttcctgcacg tatcgacgga cgaagtgttc ggctcgctgt cgccggccga tccgcagttc tccgaaacca cgccgtacgc gccgaacagc ccgtactcgg cgacgaaggc gggctccgac cacctcgtac gcgcgtatca ccacacgtac gggttgccgg tgctcacgac gaactgctcg aacaactacg gcccgtatca gttccccgag aagctgattc cgctgatgat cgcgaacgcg ctcggcggca agccgctgcc cgtctacggc gacggccaga acgtgcggga ctggctgtac gtcggcgatc actgcagcgc gattcgcgag gtgctcgcgc gcggcgtgcc gggcgagacg tacaacgtcg gcggctggaa cgaaaagaag aatctcgatg tcgtgcacac gctgtgcgat ctgctcgacg aggcccggcc gaaggcgggc ggctcgtacc gcgatcagat cacgtacgtg accgatcgcc ccggccacga tcgccgctat gcgatcgatg cgcgcaagct cgagcgcgag ctcggctgga agccggccga gacgttcgag accggacttg cgaagaccgt gcgctggtat ctcgaccatc aggagtgggt cgacgaagtg gtgtcgggcg attaccgcaa gtgggtcgaa acgaactacg cgcaacgcgc gtaaaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa