BupsA.00081.a

cysteine desulfurase IscS

CENTER ID:	BupsA.00081.a
ORGANISM:	Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE:	Melioidosis
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.00081.a.A1.GE25044	Full length( BupsA.00081.a )		1	407

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.00081.a.A1.PW25082	Full length( BupsA.00081.a )		1	407

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_126959
PATRIC ID:	fig\|320372.6.peg.2859
RefSeq:	YP_334119
UniProt:	Q3JQN4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNNDIPHLPI YMDYSATTPV DPRVVDKMVP YLREQFGNPA SRSHAYGWDA ERAVEEAREQ VAALVNADPR EIIWTSGATE SDNLAIKGAA HFYQGKGKHI VTVKTEHKAV LDTCRELERE GFEVTYLDVK DDGLIDLDVF KAALRPDTIL VSVMHVNNEI GVIQDIATIG EICREKGIIF HVDAAQATGK VEIDLAKLKV DLMSFSAHKT YGPKGIGALY VRRKPRVRIE AQMHGGGHER GMRSGTLPTH QIVGMGEAFR IAREEMATEN ERIRMLRDKL LRGLSEIDET YVNGDLEHRI PHNLNISFNF VEGESLIMAI KDVAVSSGSA CTSASLEPSY VLRALGRNDE LAHSSIRFTV GRFTTEQEVD YVIDLLKSKI AKLRDLSPLW EMHQEGIDLS TIEWAAH

NT Sequence

ATGAACAACG ATATCCCCCA CCTGCCCATC TACATGGACT ACAGCGCGAC GACCCCCGTC GATCCGCGCG TCGTCGACAA GATGGTGCCG TATCTGCGCG AGCAGTTCGG CAACCCGGCG TCGCGCAGCC ACGCATACGG CTGGGACGCG GAGCGCGCGG TCGAAGAGGC GCGCGAGCAG GTGGCCGCCC TCGTGAACGC CGATCCGCGC GAGATCATCT GGACCTCCGG CGCGACCGAG TCCGACAACC TCGCGATCAA GGGCGCCGCG CACTTCTATC AGGGCAAGGG CAAGCACATC GTCACCGTGA AGACCGAGCA CAAGGCGGTG CTCGACACCT GCCGCGAGCT CGAGCGCGAA GGCTTCGAGG TGACCTATCT CGACGTGAAG GACGACGGGC TCATCGATCT CGACGTGTTC AAGGCCGCGC TGCGCCCGGA CACGATCCTC GTCTCGGTGA TGCATGTGAA CAACGAGATC GGCGTGATCC AGGACATCGC GACGATCGGC GAGATCTGCC GCGAGAAGGG CATCATCTTC CACGTCGACG CCGCGCAGGC GACGGGCAAG GTCGAAATCG ACCTCGCGAA GCTGAAGGTC GACCTGATGT CGTTCTCCGC GCACAAGACC TACGGCCCGA AGGGCATCGG CGCGTTGTAT GTGCGCCGCA AGCCGCGCGT GCGCATCGAG GCGCAGATGC ACGGCGGCGG CCACGAGCGC GGCATGCGCT CGGGCACGTT GCCGACGCAC CAGATCGTCG GCATGGGCGA GGCGTTTCGC ATCGCGCGCG AAGAGATGGC GACCGAGAAC GAGCGCATCC GGATGCTGCG CGACAAGCTG CTGCGCGGCC TGTCGGAAAT CGACGAAACC TACGTGAACG GCGATCTCGA GCACCGGATT CCGCACAACC TGAACATCAG CTTCAATTTT GTCGAAGGCG AATCGCTGAT CATGGCGATC AAGGACGTCG CGGTGTCGTC GGGCTCCGCG TGCACGTCGG CGTCGCTCGA GCCGTCCTAC GTGCTGCGCG CGCTCGGCCG CAACGACGAG CTCGCGCACA GCTCGATCCG CTTCACGGTC GGCCGCTTCA CGACGGAGCA GGAAGTCGAC TACGTGATCG ACCTGCTGAA GAGCAAGATC GCGAAGCTGC GCGACCTGTC GCCGCTTTGG GAGATGCATC AGGAAGGCAT CGATCTGTCG ACGATCGAAT GGGCGGCGCA C

Details for BupsA.00081.a.A1.GE25044

HARVESTED ON:	2/12/2008
SEQUENCED ON:	7/29/2008
EXPECTED MW:	47kDa
OBSERVED MW:	47kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	99

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMNNDIPHLP IYMDYSATTP VDPRVVDKMV PYLREQFGNP ASRSHAYGWD AERAVEEARE QVAALVNADP REIIWTSGAT ESDNLAIKGA AHFYQGKGKH IVTVKTEHKA VLDTCRELER EGFEVTYLDV KDDGLIDLDV FKAALRPDTI LVSVMHVNNE IGVIQDIATI GEICREKGII FHVDAAQATG KVEIDLAKLK VDLMSFSAHK TYGPKGIGAL YVRRKPRVRI EAQMHGGGHE RGMRSGTLPT HQIVGMGEAF RIAREEMATE NERIRMLRDK LLRGLSEIDE TYVNGDLEHR IPHNLNISFN FVEGESLIMA IKDVAVSSGS ACTSASLEPS YVLRALGRND ELAHSSIRFT VGRFTTGQEV DYVIDLLKSK IAKLRDLSPL WEMHQEGIDL STIEWA

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaaca acgatatccc ccacctgccc atctacatgg actacagcgc gacgaccccc gtcgatccgc gcgtcgtcga caagatggtg ccgtatctgc gcgagcagtt cggcaacccg gcgtcgcgca gccacgcata cggctgggac gcggagcgcg cggtcgaaga ggcgcgcgag caggtggccg ccctcgtgaa cgccgatccg cgcgagatca tctggacctc cggcgcgacc gagtccgaca acctcgcgat caagggcgcc gcgcacttct atcagggcaa gggcaagcac atcgtcaccg tgaagaccga gcacaaggcg gtgctcgaca cctgccgcga gctcgagcgc gaaggcttcg aggtgaccta tctcgacgtg aaggacgacg ggctcatcga tctcgacgtg ttcaaggccg cgctgcgccc ggacacgatc ctcgtctcgg tgatgcatgt gaacaacgag atcggcgtga tccaggacat cgcgacgatc ggcgagatct gccgcgagaa gggcatcatc ttccacgtcg acgccgcgca ggcgacgggc aaggtcgaaa tcgacctcgc gaagctgaag gtcgacctga tgtcgttctc tgcgcacaag acctacggcc cgaagggcat cggcgcgttg tatgtgcgcc gcaagccgcg cgtgcgcatc gaggcgcaga tgcacggcgg cggccacgag cgcggcatgc gctcgggcac gttgccgacg caccagatcg tcggcatggg cgaggcgttt cgcatcgcgc gcgaagagat ggcgaccgag aacgagcgca tccggatgct gcgcgacaag ctgctgcgcg gcctgtcgga aatcgacgaa acctacgtga acggcgatct cgagcaccgg attccgcaca acctgaacat cagcttcaat tttgtcgaag gcgaatcgct gatcatggcg atcaaggacg tcgcggtgtc gtcgggctcc gcgtgcacgt cggcgtcgct cgagccgtcc tacgtgctgc gcgcgctcgg ccgcaacgac gagctcgcgc acagctcgat ccgcttcacg gtcggccgct tcacgacggg gcaggaagtc gactacgtga tcgacctgct gaagagcaag atcgcgaagc tgcgcgacct gtcgccgctt tgggagatgc atcaggaagg catcgatctg tcgacgatcg aatgggcggc g

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaacaacgat atcccccacc tgcccatcta catggactac agcgcgacga cccccgtcga tccgcgcgtc gtcgacaaga tggtgccgta tctgcgcgag cagttcggca acccggcgtc gcgcagccac gcatacggct gggacgcgga gcgcgcggtc gaagaggcgc gcgagcaggt ggccgccctc gtgaacgccg atccgcgcga gatcatctgg acctccggcg cgaccgagtc cgacaacctc gcgatcaagg gcgccgcgca cttctatcag ggcaagggca agcacatcgt caccgtgaag accgagcaca aggcggtgct cgacacctgc cgcgagctcg agcgcgaagg cttcgaggtg acctatctcg acgtgaagga cgacgggctc atcgatctcg acgtgttcaa ggccgcgctg cgcccggaca cgatcctcgt ctcggtgatg catgtgaaca acgagatcgg cgtgatccag gacatcgcga cgatcggcga gatctgccgc gagaagggca tcatcttcca cgtcgacgcc gcgcaggcga cgggcaaggt cgaaatcgac ctcgcgaagc tgaaggtcga cctgatgtcg ttctccgcgc acaagaccta cggcccgaag ggcatcggcg cgttgtatgt gcgccgcaag ccgcgcgtgc gcatcgaggc gcagatgcac ggcggcggcc acgagcgcgg catgcgctcg ggcacgttgc cgacgcacca gatcgtcggc atgggcgagg cgtttcgcat cgcgcgcgaa gagatggcga ccgagaacga gcgcatccgg atgctgcgcg acaagctgct gcgcggcctg tcggaaatcg acgaaaccta cgtgaacggc gatctcgagc accggattcc gcacaacctg aacatcagct tcaattttgt cgaaggcgaa tcgctgatca tggcgatcaa ggacgtcgcg gtgtcgtcgg gctccgcgtg cacgtcggcg tcgctcgagc cgtcctacgt gctgcgcgcg ctcggccgca acgacgagct cgcgcacagc tcgatccgct tcacggtcgg ccgcttcacg acggagcagg aagtcgacta cgtgatcgac ctgctgaaga gcaagatcgc gaagctgcgc gacctgtcgc cgctttggga gatgcatcag gaaggcatcg atctgtcgac gatcgaatgg gcggcgcact gaaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa

Details for BupsA.00081.a.A1.PW25082

PURIFICATION DATe:	4/7/2008
CONCENTRATION:	20mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	2
VIAL VOLUME:	data unavailable
PERCENT IDENTITY:	99
PERCENT COVERAGE:	99

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAACAACGAT ATCCCCCACC TGCCCATCTA CATGGACTAC AGCGCGACGA CCCCCGTCGA TCCGCGCGTC GTCGACAAGA TGGTGCCGTA TCTGCGCGAG CAGTTCGGCA ACCCGGCGTC GCGCAGCCAC GCATACGGCT GGGACGCGGA GCGCGCGGTC GAAGAGGCGC GCGAGCAGGT GGCCGCCCTC GTGAACGCCG ATCCGCGCGA GATCATCTGG ACCTCCGGCG CGACCGAGTC CGACAACCTC GCGATCAAGG GCGCCGCGCA CTTCTATCAG GGCAAGGGCA AGCACATCGT CACCGTGAAG ACCGAGCACA AGGCGGTGCT CGACACCTGC CGCGAGCTCG AGCGCGAAGG CTTCGAGGTG ACCTATCTCG ACGTGAAGGA CGACGGGCTC ATCGATCTCG ACGTGTTCAA GGCCGCGCTG CGCCCGGACA CGATCCTCGT CTCGGTGATG CATGTGAACA ACGAGATCGG CGTGATCCAG GACATCGCGA CGATCGGCGA GATCTGCCGC GAGAAGGGCA TCATCTTCCA CGTCGACGCC GCGCAGGCGA CGGGCAAGGT CGAAATCGAC CTCGCGAAGC TGAAGGTCGA CCTGATGTCG TTCTCCGCGC ACAAGACCTA CGGCCCGAAG GGCATCGGCG CGTTGTATGT GCGCCGCAAG CCGCGCGTGC GCATCGAGGC GCAGATGCAC GGCGGCGGCC ACGAGCGCGG CATGCGCTCG GGCACGTTGC CGACGCACCA GATCGTCGGC ATGGGCGAGG CGTTTCGCAT CGCGCGCGAA GAGATGGCGA CCGAGAACGA GCGCATCCGG ATGCTGCGCG ACAAGCTGCT GCGCGGCCTG TCGGAAATCG ACGAAACCTA CGTGAACGGC GATCTCGAGC ACCGGATTCC GCACAACCTG AACATCAGCT TCAATTTTGT CGAAGGCGAA TCGCTGATCA TGGCGATCAA GGACGTCGCG GTGTCGTCGG GCTCCGCGTG CACGTCGGCG TCGCTCGAGC CGTCCTACGT GCTGCGCGCG CTCGGCCGCA ACGACGAGCT CGCGCACAGC TCGATCCGCT TCACGGTCGG CCGCTTCACG ACGGAGCAGG AAGTCGACTA CGTGATCGAC CTGCTGAAGA GCAAGATCGC GAAGCTGCGC GACCTGTCGC CGCTTTGGGA GATGCATCAG GAAGGCATCG ATCTGTCGAC GATCGAATGG GCGGCGCACT GAAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA