BupsA.00075.a

delta-aminolevulinic acid dehydratase

CENTER ID: BupsA.00075.a
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: native diffraction-data
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.00075.a.A1.GE25040 Full length( BupsA.00075.a ) 1 354
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.00075.a.A1.PW25078 Full length( BupsA.00075.a ) 1 354
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_127486
PATRIC ID: fig|320372.6.peg.3892
RefSeq: YP_335110
UniProt: Q3JMU3
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSQKFTEGNC KVSSVTYPSH SAMSIHPLHR PRRMRRDDFS RRLMRENILT TNDLIYPVFV VEGTNVRQPV PSMPGVERVS IDLLMGVAEQ CVELGVPVLS LFPAIEPSLK TPDGREAANP EGLIPRAVRE LKRRFPELGV LTDVALDPYT SHGQDGVLDE AGHVLNDETL EILVEQARAQ AEAGVDIVAP SDMMDGRIGA VREMLEREGH IYTRIMAYSA KYASAFYGPF RDAVGSASNL GKGNKMTYQM DPANSDEALR EVRLDIDEGA DMVMVKPGMP YLDIVRRVKD EFRYPTYVYQ VSGEYAMLKA AAQNGWLDHD KVVLESLLAF KRAGADGILT YFALDAARLL RAQK
NT Sequence
TTGTCTCAAA AGTTTACCGA AGGGAACTGT AAGGTTTCGT CGGTTACCTA TCCCAGCCAT TCCGCCATGA GCATCCATCC CCTGCACCGC CCGCGCCGCA TGCGCCGCGA CGATTTCTCG CGACGCCTGA TGCGCGAAAA TATCCTCACC ACGAACGATC TGATCTACCC GGTGTTCGTC GTCGAAGGCA CGAACGTGCG CCAGCCGGTG CCGTCGATGC CGGGCGTCGA GCGCGTGTCG ATCGATCTGC TGATGGGTGT CGCCGAGCAA TGCGTCGAGC TCGGCGTGCC GGTCCTGTCG CTCTTTCCGG CCATCGAGCC GTCGCTGAAG ACGCCCGACG GCCGCGAAGC GGCCAATCCC GAAGGGCTGA TCCCGCGTGC GGTACGCGAG CTGAAGCGCC GCTTCCCCGA ACTCGGCGTG CTGACCGACG TCGCGCTCGA TCCGTACACG AGCCACGGCC AGGACGGCGT GCTCGACGAG GCCGGCCATG TGCTCAACGA CGAAACGCTC GAGATTCTCG TCGAGCAGGC GCGCGCGCAG GCCGAAGCGG GCGTCGACAT CGTCGCGCCG TCGGACATGA TGGACGGGCG CATCGGCGCG GTGCGCGAGA TGCTCGAGCG TGAAGGCCAC ATCTATACGC GGATCATGGC CTACTCGGCG AAGTACGCGT CAGCGTTCTA CGGCCCGTTC CGCGACGCGG TCGGCTCCGC GTCGAATCTC GGCAAGGGCA ACAAGATGAC CTACCAGATG GACCCCGCGA ACAGCGACGA GGCGCTGCGC GAAGTGCGCC TCGACATCGA CGAGGGCGCG GACATGGTCA TGGTGAAGCC CGGCATGCCG TATCTCGACA TCGTGCGCCG CGTGAAGGAC GAATTCCGCT ATCCGACCTA CGTCTATCAG GTGAGCGGCG AATACGCGAT GCTGAAGGCC GCCGCGCAGA ACGGCTGGCT CGATCACGAC AAAGTCGTGC TCGAATCGCT GCTCGCGTTC AAGCGCGCGG GCGCGGACGG CATTCTCACG TACTTCGCGC TCGACGCGGC GCGGCTGCTG CGCGCGCAGA AG
Details for BupsA.00075.a.A1.GE25040
HARVESTED ON: 2/12/2008
SEQUENCED ON: 8/6/2008
EXPECTED MW: 41kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMLSQKFTEG NCKVSSVTYP SHSAMSIHPL HRPRRMRRDD FSRRLMRENI LTTNDLIYPV FVVEGTNVRQ PVPSMPGVER VSIDLLMGVA EQCVELGVPV LSLFPAIEPS LKTPDGREAA NPEGLIPRAV RELKRRFPEL GVLTDVALDP YTSHGQDGVL DEAGHVLNDE TLEILVEQAR AQAEAGVDIV APSDMMDGRI GAVREMLERE GHIYTRIMAY SAKYASAFYG PFRDAVGSAS NLGKGNKMTY QMDPANSDEA LREVRLDIDE GADMVMVKPG MPYLDIVRRV KDEFRYPTYV YQVSGEYAML KAAAQNGWLD HDKVVLESLL AFKRAGADGI LTYFALDAAR LLR
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgttgt ctcaaaagtt taccgaaggg aactgtaagg tttcgtcggt tacctatccc agccattccg ccatgagcat ccatcccctg caccgcccgc gccgcatgcg ccgcgacgat ttctcgcgac gcctgatgcg cgaaaatatc ctcaccacga acgatctgat ctacccggtg ttcgtcgtcg aaggcacgaa cgtgcgccag ccggtgccgt cgatgccggg cgtcgagcgc gtgtcgatcg atctgctgat gggtgtcgcc gagcaatgcg tcgagctcgg cgtgccggtc ctgtcgctct ttccggccat cgagccgtcg ctgaagacgc ccgacggccg cgaagcggcc aatcccgaag ggctgatccc gcgtgcggta cgcgagctga agcgccgctt ccccgaactc ggcgtgctga ccgacgtcgc gctcgatccg tacacgagcc acggccagga cggcgtgctc gacgaggccg gccatgtgct caacgacgaa acgctcgaga ttctcgtcga gcaggcgcgc gcgcaggccg aagcgggcgt cgacatcgtc gcgccgtcgg acatgatgga cgggcgcatc ggcgcggtgc gcgagatgct cgagcgtgaa ggccacatct atacgcggat catggcctac tcggcgaagt acgcgtcagc gttctacggc ccgttccgcg acgcggtcgg ctccgcgtcg aatctcggca agggcaacaa gatgacctac cagatggacc ccgcgaacag cgacgaggcg ctgcgcgaag tgcgcctcga catcgacgag ggcgcggaca tggtcatggt gaagcccggc atgccgtatc tcgacatcgt gcgccgcgtg aaggacgaat tccgctatcc gacctacgtc tatcaggtga gcggcgaata cgcgatgctg aaggccgccg cgcagaacgg ctggctcgat cacgacaaag tcgtgctcga atcgctgctc gcgttcaagc gcgcgggcgc ggacggcatt ctcacgtact tcgcgctcga cgcggcgcgg ctgctgcgcg cg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLSQKFTEG NCKVSSVTYP SHSAMSIHPL HRPRRMRRDD FSRRLMRENI LTTNDLIYPV FVVEGTNVRQ PVPSMPGVER VSIDLLMGVA EQCVELGVPV LSLFPAIEPS LKTPDGREAA NPEGLIPRAV RELKRRFPEL GVLTDVALDP YTSHGQDGVL DEAGHVLNDE TLEILVEQAR AQAEAGVDIV APSDMMDGRI GAVREMLERE GHIYTRIMAY SAKYASAFYG PFRDAVGSAS NLGKGNKMTY QMDPANSDEA LREVRLDIDE GADMVMVKPG MPYLDIVRRV KDEFRYPTYV YQVSGEYAML KAAAQNGWLD HDKVVLESLL AFKRAGADGI LTYFALDAAR LLRAQK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttgtctcaa aagtttaccg aagggaactg taaggtttcg tcggttacct atcccagcca ttccgccatg agcatccatc ccctgcaccg cccgcgccgc atgcgccgcg acgatttctc gcgacgcctg atgcgcgaaa atatcctcac cacgaacgat ctgatctacc cggtgttcgt cgtcgaaggc acgaacgtgc gccagccggt gccgtcgatg ccgggcgtcg agcgcgtgtc gatcgatctg ctgatgggtg tcgccgagca atgcgtcgag ctcggcgtgc cggtcctgtc gctctttccg gccatcgagc cgtcgctgaa gacgcccgac ggccgcgaag cggccaatcc cgaagggctg atcccgcgtg cggtacgcga gctgaagcgc cgcttccccg aactcggcgt gctgaccgac gtcgcgctcg atccgtacac gagccacggc caggacggcg tgctcgacga ggccggccat gtgctcaacg acgaaacgct cgagattctc gtcgagcagg cgcgcgcgca ggccgaagcg ggcgtcgaca tcgtcgcgcc gtcggacatg atggacgggc gcatcggcgc ggtgcgcgag atgctcgagc gtgaaggcca catctatacg cggatcatgg cctactcggc gaagtacgcg tcagcgttct acggcccgtt ccgcgacgcg gtcggctccg cgtcgaatct cggcaagggc aacaagatga cctaccagat ggaccccgcg aacagcgacg aggcgctgcg cgaagtgcgc ctcgacatcg acgagggcgc ggacatggtc atggtgaagc ccggcatgcc gtatctcgac atcgtgcgcc gcgtgaagga cgaattccgc tatccgacct acgtctatca ggtgagcggc gaatacgcga tgctgaaggc cgccgcgcag aacggctggc tcgatcacga caaagtcgtg ctcgaatcgc tgctcgcgtt caagcgcgcg ggcgcggacg gcattctcac gtacttcgcg ctcgacgcgg cgcggctgct gcgcgcgcag aagtaaaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for BupsA.00075.a.A1.PW25078
PURIFICATION DATe: 2/27/2008
CONCENTRATION: 9.1mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 29
VIAL VOLUME: 50µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMLSQKFTEG NCKVSSVTYP SHSAMSIHPL HRPRRMRRDD FSRRLMRENI LTTNDLIYPV FVVEGTNVRQ PVPSMPGVER VSIDLLMGVA EQCVELGVPV LSLFPAIEPS LKTPDGREAA NPEGLIPRAV RELKRRFPEL GVLTDVALDP YTSHGQDGVL DEAGHVLNDE TLEILVEQAR AQAEAGVDIV APSDMMDGRI GAVREMLERE GHIYTRIMAY SAKYASAFYG PFRDAVGSAS NLGKGNKMTY QMDPANSDEA LREVRLDIDE GADMVMVKPG MPYLDIVRRV KDEFRYPTYV YQVSGEYAML KAAAQNGWLD HDKVVLESLL AFKRAGADGI LTYFALDAAR LLR
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgttgt ctcaaaagtt taccgaaggg aactgtaagg tttcgtcggt tacctatccc agccattccg ccatgagcat ccatcccctg caccgcccgc gccgcatgcg ccgcgacgat ttctcgcgac gcctgatgcg cgaaaatatc ctcaccacga acgatctgat ctacccggtg ttcgtcgtcg aaggcacgaa cgtgcgccag ccggtgccgt cgatgccggg cgtcgagcgc gtgtcgatcg atctgctgat gggtgtcgcc gagcaatgcg tcgagctcgg cgtgccggtc ctgtcgctct ttccggccat cgagccgtcg ctgaagacgc ccgacggccg cgaagcggcc aatcccgaag ggctgatccc gcgtgcggta cgcgagctga agcgccgctt ccccgaactc ggcgtgctga ccgacgtcgc gctcgatccg tacacgagcc acggccagga cggcgtgctc gacgaggccg gccatgtgct caacgacgaa acgctcgaga ttctcgtcga gcaggcgcgc gcgcaggccg aagcgggcgt cgacatcgtc gcgccgtcgg acatgatgga cgggcgcatc ggcgcggtgc gcgagatgct cgagcgtgaa ggccacatct atacgcggat catggcctac tcggcgaagt acgcgtcagc gttctacggc ccgttccgcg acgcggtcgg ctccgcgtcg aatctcggca agggcaacaa gatgacctac cagatggacc ccgcgaacag cgacgaggcg ctgcgcgaag tgcgcctcga catcgacgag ggcgcggaca tggtcatggt gaagcccggc atgccgtatc tcgacatcgt gcgccgcgtg aaggacgaat tccgctatcc gacctacgtc tatcaggtga gcggcgaata cgcgatgctg aaggccgccg cgcagaacgg ctggctcgat cacgacaaag tcgtgctcga atcgctgctc gcgttcaagc gcgcgggcgc ggacggcatt ctcacgtact tcgcgctcga cgcggcgcgg ctgctgcgcg cg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLSQKFTEG NCKVSSVTYP SHSAMSIHPL HRPRRMRRDD FSRRLMRENI LTTNDLIYPV FVVEGTNVRQ PVPSMPGVER VSIDLLMGVA EQCVELGVPV LSLFPAIEPS LKTPDGREAA NPEGLIPRAV RELKRRFPEL GVLTDVALDP YTSHGQDGVL DEAGHVLNDE TLEILVEQAR AQAEAGVDIV APSDMMDGRI GAVREMLERE GHIYTRIMAY SAKYASAFYG PFRDAVGSAS NLGKGNKMTY QMDPANSDEA LREVRLDIDE GADMVMVKPG MPYLDIVRRV KDEFRYPTYV YQVSGEYAML KAAAQNGWLD HDKVVLESLL AFKRAGADGI LTYFALDAAR LLRAQK
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTTGTCTCAA AAGTTTACCG AAGGGAACTG TAAGGTTTCG TCGGTTACCT ATCCCAGCCA TTCCGCCATG AGCATCCATC CCCTGCACCG CCCGCGCCGC ATGCGCCGCG ACGATTTCTC GCGACGCCTG ATGCGCGAAA ATATCCTCAC CACGAACGAT CTGATCTACC CGGTGTTCGT CGTCGAAGGC ACGAACGTGC GCCAGCCGGT GCCGTCGATG CCGGGCGTCG AGCGCGTGTC GATCGATCTG CTGATGGGTG TCGCCGAGCA ATGCGTCGAG CTCGGCGTGC CGGTCCTGTC GCTCTTTCCG GCCATCGAGC CGTCGCTGAA GACGCCCGAC GGCCGCGAAG CGGCCAATCC CGAAGGGCTG ATCCCGCGTG CGGTACGCGA GCTGAAGCGC CGCTTCCCCG AACTCGGCGT GCTGACCGAC GTCGCGCTCG ATCCGTACAC GAGCCACGGC CAGGACGGCG TGCTCGACGA GGCCGGCCAT GTGCTCAACG ACGAAACGCT CGAGATTCTC GTCGAGCAGG CGCGCGCGCA GGCCGAAGCG GGCGTCGACA TCGTCGCGCC GTCGGACATG ATGGACGGGC GCATCGGCGC GGTGCGCGAG ATGCTCGAGC GTGAAGGCCA CATCTATACG CGGATCATGG CCTACTCGGC GAAGTACGCG TCAGCGTTCT ACGGCCCGTT CCGCGACGCG GTCGGCTCCG CGTCGAATCT CGGCAAGGGC AACAAGATGA CCTACCAGAT GGACCCCGCG AACAGCGACG AGGCGCTGCG CGAAGTGCGC CTCGACATCG ACGAGGGCGC GGACATGGTC ATGGTGAAGC CCGGCATGCC GTATCTCGAC ATCGTGCGCC GCGTGAAGGA CGAATTCCGC TATCCGACCT ACGTCTATCA GGTGAGCGGC GAATACGCGA TGCTGAAGGC CGCCGCGCAG AACGGCTGGC TCGATCACGA CAAAGTCGTG CTCGAATCGC TGCTCGCGTT CAAGCGCGCG GGCGCGGACG GCATTCTCAC GTACTTCGCG CTCGACGCGG CGCGGCTGCT GCGCGCGCAG AAGTAAAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA