BupsA.00064.a
adenylate kinase
| CENTER ID: | BupsA.00064.a |
| ORGANISM: | Burkholderia pseudomallei 1710b |
| ASSOCIATED DISEASE: | Melioidosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BupsA.00064.a.B1.GE25129 | Full length( BupsA.00064.a ) | 1 | 220 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
3GMT
DEPOSITED: 3/15/2009
DETERMINATION: XRay
CLONE: BupsA.00064.a.B1.GE25129
PROTEIN: BupsA.00064.a.B1.PB00019
Publications by SSGCID
Structural characterization of Burkholderia pseudomallei adenylate kinase (Adk): profound asymmetry in the crystal structure of the ''open'' state.
Abendroth J, Buchko GW, Myler PJ, Robinson H, Staker BL
Biochem. Biophys. Res. Commun. - 2010
volume 394, issue 4, pages 1012-7
PMID: 20331978; PMCID: PMC2856777
PDB:
3GMT
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_126762 |
| PATRIC ID: | fig|320372.6.peg.1097 |
| RefSeq: | YP_332492 |
| UniProt: | Q3JVB1 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRLILLGAPG AGKGTQANFI KEKFGIPQIS TGDMLRAAVK AGTPLGVEAK TYMDEGKLVP DSLIIGLVKE RLKEADCANG YLFDGFPRTI AQADAMKEAG VAIDYVLEID VPFSEIIERM SGRRTHPASG RTYHVKFNPP KVEGKDDVTG EPLVQRDDDK EETVKKRLDV YEAQTKPLIT YYGDWARRGA ENGLKAPAYR KISGLGAVEE IRARVFDALK
NT Sequence
ATGCGTTTGA TCCTGTTGGG CGCGCCCGGC GCGGGAAAGG GCACCCAGGC AAACTTCATC AAGGAGAAGT TCGGCATCCC GCAAATCTCG ACGGGCGACA TGCTGCGCGC CGCCGTGAAG GCCGGCACGC CGCTCGGCGT CGAGGCGAAG ACGTACATGG ACGAAGGCAA GCTCGTGCCG GATTCGCTGA TCATCGGCCT CGTCAAGGAA CGTCTGAAGG AAGCGGACTG CGCGAACGGC TATCTGTTCG ACGGCTTCCC GCGCACGATC GCGCAGGCCG ACGCGATGAA GGAAGCGGGC GTCGCGATCG ACTACGTGCT GGAGATCGAC GTGCCGTTCT CGGAAATCAT CGAACGCATG AGCGGCCGCC GCACGCATCC GGCGTCGGGC CGCACGTATC ACGTGAAGTT CAACCCGCCG AAGGTCGAGG GCAAGGACGA CGTCACAGGC GAGCCGCTGG TCCAGCGCGA CGACGACAAG GAAGAGACGG TGAAGAAACG CCTCGACGTG TACGAGGCGC AGACGAAGCC GCTCATCACG TACTACGGCG ACTGGGCGCG GCGCGGCGCG GAAAACGGCC TGAAGGCGCC CGCTTACCGG AAGATCTCCG GCCTCGGCGC CGTCGAGGAA ATCCGCGCGC GCGTGTTCGA CGCGCTCAAG
Details for BupsA.00064.a.B1.GE25129
| HARVESTED ON: | 2/26/2008 |
| SEQUENCED ON: | 1/6/2009 |
| EXPECTED MW: | 25kDa |
| OBSERVED MW: | 26kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with sequence variation |
| PERCENT IDENTITY: | 98 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMR LILLGAPGAG KGTQANFIKE KFGIPQISTG DMLRAAVKAG TPLGVEAKTY MDEGKLVPDS LIIGLVKERL KEADCANGYL FDGFPRTIAQ ADAMKEAGVA IDYVLEIDVP FSEIIERMSG RRTHPASGRT YHVKFNPPKV EGKDDVTGEP LVQRDDDKEE TVKKRLDVYE AQTKPLITYY GDWARRGAEN GLKAPAYRKI SGLGAVEEIR ARVRRAQVSE
Validated NT Sequence
atggctcacc accaccacca ccatatgcgt ttgatcctgt tgggcgcgcc cggcgcggga aagggcaccc aggcaaactt catcaaggag aagttcggca tcccgcaaat ctcgacgggc gacatgctgc gcgccgccgt gaaggccggc acgccgctcg gcgtcgaggc gaagacgtac atggacgaag gcaagctcgt gccggattcg ctgatcatcg gcctcgtcaa ggaacgtctg aaggaagcgg actgcgcgaa cggctatctg ttcgacggct tcccgcgcac gatcgcgcag gccgacgcga tgaaggaagc gggcgtcgcg atcgactacg tgctggagat cgacgtgccg ttctcggaaa tcatcgaacg catgagcggc cgccgcacgc atccggcgtc gggccgcacg tatcacgtga agttcaaccc gccgaaggtc gagggcaagg acgacgtcac aggcgagccg ctggtccagc gcgacgacga caaggaagag acggtgaaga aacgcctcga cgtgtacgag gcgcagacga agccgctcat cacgtactac ggcgactggg cgcggcgcgg cgcggaaaac ggcctgaagg cgcccgctta ccggaagatc tccggcctcg gcgccgtcga ggaaatccgc gcgcgtgttc gacgcgctca agtaagtgag taagat
Expected Protein Sequence
MAHHHHHHMR LILLGAPGAG KGTQANFIKE KFGIPQISTG DMLRAAVKAG TPLGVEAKTY MDEGKLVPDS LIIGLVKERL KEADCANGYL FDGFPRTIAQ ADAMKEAGVA IDYVLEIDVP FSEIIERMSG RRTHPASGRT YHVKFNPPKV EGKDDVTGEP LVQRDDDKEE TVKKRLDVYE AQTKPLITYY GDWARRGAEN GLKAPAYRKI SGLGAVEEIR ARVFDALK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcgtt tgatcctgtt gggcgcgccc ggcgcgggaa agggcaccca ggcaaacttc atcaaggaga agttcggcat cccgcaaatc tcgacgggcg acatgctgcg cgccgccgtg aaggccggca cgccgctcgg cgtcgaggcg aagacgtaca tggacgaagg caagctcgtg ccggattcgc tgatcatcgg cctcgtcaag gaacgtctga aggaagcgga ctgcgcgaac ggctatctgt tcgacggctt cccgcgcacg atcgcgcagg ccgacgcgat gaaggaagcg ggcgtcgcga tcgactacgt gctggagatc gacgtgccgt tctcggaaat catcgaacgc atgagcggcc gccgcacgca tccggcgtcg ggccgcacgt atcacgtgaa gttcaacccg ccgaaggtcg agggcaagga cgacgtcaca ggcgagccgc tggtccagcg cgacgacgac aaggaagaga cggtgaagaa acgcctcgac gtgtacgagg cgcagacgaa gccgctcatc acgtactacg gcgactgggc gcggcgcggc gcggaaaacg gcctgaaggc gcccgcttac cggaagatct ccggcctcgg cgccgtcgag gaaatccgcg cgcgcgtgtt cgacgcgctc aagtgatgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t