BupsA.00063.a

histidyl-tRNA synthetase

CENTER ID: BupsA.00063.a
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.00063.a.A1.GE25032 Full length( BupsA.00063.a ) 1 446
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.00063.a.A1.PW25070 Full length( BupsA.00063.a ) 1 446
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_126806
PATRIC ID: fig|320372.6.peg.2462
RefSeq: YP_333748
UniProt: Q3JRQ5
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTEQKRKLEK LTGVKGMNDI LPQDAGLWEF FEATVKSLLR AYGYQNIRTP IVEHTQLFTR GIGEVTDIVE KEMYSFVDAL NGENLTLRPE NTAAVVRAAI EHNMLYDGPK RLWYLGPMFR HERPQRGRYR QFHQVGVEAL GFAGPDADAE IIMMCQRLWD DLGLTGIKLE INSLGLAEER AAHRVELIKY LEQHVDKLDD DAQRRLYTNP LRVLDTKNPA LQEIVRNAPQ LIDFLGDVSR AHFDGLQQLL KANNLPFTIN PRLVRGLDYY NLTVFEWVTD KLGAQGTVAA GGRYDPLIEQ LGGKPTAACG WAMGVERILE LLKEEHLVPE QEGVDVYVVH QGDAAREQAF IVAERLRDTG LDVILHCSAD GAGASFKSQM KRADASGAAF AVIFGEDEVA NGTVSVKPLR GTGAEGEKNV QQSVPVESLT EFLINAMVAT AEDGDD
NT Sequence
ATGACAGAAC AAAAGCGAAA GCTCGAGAAG CTGACGGGCG TGAAGGGCAT GAACGACATC CTCCCGCAGG ATGCCGGCTT GTGGGAATTC TTCGAGGCGA CGGTGAAGTC GCTGCTGCGC GCATACGGCT ATCAGAACAT CCGCACGCCG ATCGTCGAGC ATACGCAGCT CTTCACGCGC GGCATCGGCG AGGTGACCGA CATCGTCGAA AAGGAGATGT ACAGCTTCGT CGATGCGTTG AACGGCGAGA ACCTGACGCT GCGCCCCGAG AACACCGCGG CCGTCGTGCG CGCGGCGATC GAGCACAACA TGCTGTATGA CGGCCCGAAA CGCCTGTGGT ATCTCGGGCC GATGTTCCGC CACGAGCGCC CGCAGCGCGG CCGTTATCGC CAGTTCCATC AGGTCGGCGT CGAGGCGCTC GGCTTCGCGG GCCCGGACGC GGACGCGGAA ATCATCATGA TGTGCCAGCG CCTGTGGGAC GATCTCGGCC TCACCGGCAT CAAGCTCGAG ATCAACTCGC TCGGTCTCGC CGAGGAGCGC GCCGCGCACC GCGTCGAGCT CATCAAGTAT CTCGAGCAGC ACGTCGACAA GCTCGACGAC GACGCGCAGC GCCGCCTCTA CACCAACCCG CTGCGCGTGC TCGACACGAA GAATCCGGCG CTGCAGGAGA TCGTGCGGAA CGCGCCGCAG CTGATCGATT TCCTCGGCGA CGTGTCGCGC GCGCACTTCG ACGGCTTGCA GCAGCTGCTG AAGGCGAACA ACCTGCCGTT TACGATCAAT CCGCGGCTCG TGCGCGGGCT CGACTACTAC AACCTGACCG TGTTCGAGTG GGTGACCGAC AAGCTCGGCG CGCAGGGCAC GGTCGCCGCG GGCGGCCGCT ACGATCCGCT GATCGAGCAG TTGGGCGGCA AGCCGACCGC CGCGTGCGGC TGGGCGATGG GTGTCGAGCG CATCCTCGAG CTCCTGAAGG AAGAGCACCT CGTGCCGGAG CAGGAAGGCG TCGACGTGTA CGTCGTCCAT CAGGGCGACG CGGCGCGCGA GCAGGCGTTC ATCGTCGCCG AGCGTCTGCG CGACACCGGC CTCGACGTGA TCCTGCATTG CAGCGCGGAC GGCGCGGGCG CGAGCTTCAA GTCGCAGATG AAGCGCGCGG ATGCAAGCGG CGCGGCGTTC GCGGTGATCT TCGGCGAAGA CGAGGTCGCG AACGGCACGG TGAGCGTGAA GCCGCTGCGC GGCACGGGCG CCGAAGGCGA GAAGAACGTT CAGCAGTCCG TGCCGGTCGA AAGCTTGACC GAATTTCTAA TCAATGCGAT GGTTGCAACC GCCGAAGACG GCGACGAC
Details for BupsA.00063.a.A1.GE25032
HARVESTED ON: 2/12/2008
SEQUENCED ON: 7/29/2008
EXPECTED MW: 51kDa
OBSERVED MW: 51kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTEQKRKLE KLTGVKGMND ILPQDAGLWE FFEATVKSLL RAYGYQNIRT PIVEHTQLFT RGIGEVTDIV EKEMYSFVDA LNGENLTLRP ENTAAVVRAA IEHNMLYDGP KRLWYLGPMF RHERPQRGRY RQFHQVGVEA LGFAGPDADA EIIMMCQRLW DDLGLTGIKL EINSLGLAEE RAAHRVELIK YLEQHVDKLD DDAQRRLYTN PLRVLDTKNP ALQEIVRNAP QLIDFLGDVS RAHFDGLQQL LKANNLPFTI NPRLVRGLDY YNLTVFEWVT DKLGAQGTVA AGGRYDPLIE QLGGKPTAAC GWAMGVERIL ELLKEEHLVP EQEGVDVYVV HQGDAAREQA FIVAERLRDT GLDVILHCSA DGAGASFKSQ MKRADASGAA FAVIFGEDEV ANGTVSVKPL RGTGAEGEKN VQQSVPVESL TEFLINAMVA TAEDG
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgacag aacaaaagcg aaagctcgag aagctgacgg gcgtgaaggg catgaacgac atcctcccgc aggatgccgg cttgtgggaa ttcttcgagg cgacggtgaa gtcgctgctg cgcgcatacg gctatcagaa catccgcacg ccgatcgtcg agcatacgca gctcttcacg cgcggcatcg gcgaggtgac cgacatcgtc gaaaaggaga tgtacagctt cgtcgatgcg ttgaacggcg agaacctgac gctgcgcccc gagaacaccg cggccgtcgt gcgcgcggcg atcgagcaca acatgctgta tgacggcccg aaacgcctgt ggtatctcgg gccgatgttc cgccacgagc gcccgcagcg cggccgttat cgccagttcc atcaggtcgg cgtcgaggcg ctcggcttcg cgggcccgga cgcggacgcg gaaatcatca tgatgtgcca gcgcctgtgg gacgatctcg gcctcaccgg catcaagctc gagatcaact cgctcggtct cgccgaggag cgcgccgcgc accgcgtcga gctcatcaag tatctcgagc agcacgtcga caagctcgac gacgacgcgc agcgccgcct ctacaccaac ccgctgcgcg tgctcgacac gaagaatccg gcgctgcagg agatcgtgcg gaacgcgccg cagctgatcg atttcctcgg cgacgtgtcg cgcgcgcact tcgacggctt gcagcagctg ctgaaggcga acaacctgcc gtttacgatc aatccgcggc tcgtgcgcgg gctcgactac tacaacctga ccgtgttcga gtgggtgacc gacaagctcg gcgcgcaggg cacggtcgcc gcgggcggcc gctacgatcc gctgatcgag cagttgggcg gcaagccgac cgccgcgtgc ggctgggcga tgggtgtcga gcgcatcctc gagctcctga aggaagagca cctcgtgccg gagcaggaag gcgtcgacgt gtacgtcgtc catcagggcg acgcggcgcg cgagcaggcg ttcatcgtcg ccgagcgtct gcgcgacacc ggcctcgacg tgatcctgca ttgcagcgcg gacggcgcgg gcgcgagctt caagtcgcag atgaagcgcg cggatgcaag cggcgcggcg ttcgcggtga tcttcggcga agacgaggtc gcgaacggca cggtgagcgt gaagccgctg cgcggcacgg gcgccgaagg cgagaagaac gttcagcagt ccgtgccggt cgaaagcttg accgaatttc taatcaatgc gatggttgca accgccgaag acggcg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTEQKRKLE KLTGVKGMND ILPQDAGLWE FFEATVKSLL RAYGYQNIRT PIVEHTQLFT RGIGEVTDIV EKEMYSFVDA LNGENLTLRP ENTAAVVRAA IEHNMLYDGP KRLWYLGPMF RHERPQRGRY RQFHQVGVEA LGFAGPDADA EIIMMCQRLW DDLGLTGIKL EINSLGLAEE RAAHRVELIK YLEQHVDKLD DDAQRRLYTN PLRVLDTKNP ALQEIVRNAP QLIDFLGDVS RAHFDGLQQL LKANNLPFTI NPRLVRGLDY YNLTVFEWVT DKLGAQGTVA AGGRYDPLIE QLGGKPTAAC GWAMGVERIL ELLKEEHLVP EQEGVDVYVV HQGDAAREQA FIVAERLRDT GLDVILHCSA DGAGASFKSQ MKRADASGAA FAVIFGEDEV ANGTVSVKPL RGTGAEGEKN VQQSVPVESL TEFLINAMVA TAEDGDD
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacagaacaa aagcgaaagc tcgagaagct gacgggcgtg aagggcatga acgacatcct cccgcaggat gccggcttgt gggaattctt cgaggcgacg gtgaagtcgc tgctgcgcgc atacggctat cagaacatcc gcacgccgat cgtcgagcat acgcagctct tcacgcgcgg catcggcgag gtgaccgaca tcgtcgaaaa ggagatgtac agcttcgtcg atgcgttgaa cggcgagaac ctgacgctgc gccccgagaa caccgcggcc gtcgtgcgcg cggcgatcga gcacaacatg ctgtatgacg gcccgaaacg cctgtggtat ctcgggccga tgttccgcca cgagcgcccg cagcgcggcc gttatcgcca gttccatcag gtcggcgtcg aggcgctcgg cttcgcgggc ccggacgcgg acgcggaaat catcatgatg tgccagcgcc tgtgggacga tctcggcctc accggcatca agctcgagat caactcgctc ggtctcgccg aggagcgcgc cgcgcaccgc gtcgagctca tcaagtatct cgagcagcac gtcgacaagc tcgacgacga cgcgcagcgc cgcctctaca ccaacccgct gcgcgtgctc gacacgaaga atccggcgct gcaggagatc gtgcggaacg cgccgcagct gatcgatttc ctcggcgacg tgtcgcgcgc gcacttcgac ggcttgcagc agctgctgaa ggcgaacaac ctgccgttta cgatcaatcc gcggctcgtg cgcgggctcg actactacaa cctgaccgtg ttcgagtggg tgaccgacaa gctcggcgcg cagggcacgg tcgccgcggg cggccgctac gatccgctga tcgagcagtt gggcggcaag ccgaccgccg cgtgcggctg ggcgatgggt gtcgagcgca tcctcgagct cctgaaggaa gagcacctcg tgccggagca ggaaggcgtc gacgtgtacg tcgtccatca gggcgacgcg gcgcgcgagc aggcgttcat cgtcgccgag cgtctgcgcg acaccggcct cgacgtgatc ctgcattgca gcgcggacgg cgcgggcgcg agcttcaagt cgcagatgaa gcgcgcggat gcaagcggcg cggcgttcgc ggtgatcttc ggcgaagacg aggtcgcgaa cggcacggtg agcgtgaagc cgctgcgcgg cacgggcgcc gaaggcgaga agaacgttca gcagtccgtg ccggtcgaaa gcttgaccga atttctaatc aatgcgatgg ttgcaaccgc cgaagacggc gacgactgaa aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a
Details for BupsA.00063.a.A1.PW25070
PURIFICATION DATe: 5/1/2008
CONCENTRATION: 20.4mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 14
VIAL VOLUME: 50µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTEQKRKLE KLTGVKGMND ILPQDAGLWE FFEATVKSLL RAYGYQNIRT PIVEHTQLFT RGIGEVTDIV EKEMYSFVDA LNGENLTLRP ENTAAVVRAA IEHNMLYDGP KRLWYLGPMF RHERPQRGRY RQFHQVGVEA LGFAGPDADA EIIMMCQRLW DDLGLTGIKL EINSLGLAEE RAAHRVELIK YLEQHVDKLD DDAQRRLYTN PLRVLDTKNP ALQEIVRNAP QLIDFLGDVS RAHFDGLQQL LKANNLPFTI NPRLVRGLDY YNLTVFEWVT DKLGAQGTVA AGGRYDPLIE QLGGKPTAAC GWAMGVERIL ELLKEEHLVP EQEGVDVYVV HQGDAAREQA FIVAERLRDT GLDVILHCSA DGAGASFKSQ MKRADASGAA FAVIFGEDEV ANGTVSVKPL RGTGAEGEKN VQQSVPVESL TEFLINAMVA TAEDG
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgacag aacaaaagcg aaagctcgag aagctgacgg gcgtgaaggg catgaacgac atcctcccgc aggatgccgg cttgtgggaa ttcttcgagg cgacggtgaa gtcgctgctg cgcgcatacg gctatcagaa catccgcacg ccgatcgtcg agcatacgca gctcttcacg cgcggcatcg gcgaggtgac cgacatcgtc gaaaaggaga tgtacagctt cgtcgatgcg ttgaacggcg agaacctgac gctgcgcccc gagaacaccg cggccgtcgt gcgcgcggcg atcgagcaca acatgctgta tgacggcccg aaacgcctgt ggtatctcgg gccgatgttc cgccacgagc gcccgcagcg cggccgttat cgccagttcc atcaggtcgg cgtcgaggcg ctcggcttcg cgggcccgga cgcggacgcg gaaatcatca tgatgtgcca gcgcctgtgg gacgatctcg gcctcaccgg catcaagctc gagatcaact cgctcggtct cgccgaggag cgcgccgcgc accgcgtcga gctcatcaag tatctcgagc agcacgtcga caagctcgac gacgacgcgc agcgccgcct ctacaccaac ccgctgcgcg tgctcgacac gaagaatccg gcgctgcagg agatcgtgcg gaacgcgccg cagctgatcg atttcctcgg cgacgtgtcg cgcgcgcact tcgacggctt gcagcagctg ctgaaggcga acaacctgcc gtttacgatc aatccgcggc tcgtgcgcgg gctcgactac tacaacctga ccgtgttcga gtgggtgacc gacaagctcg gcgcgcaggg cacggtcgcc gcgggcggcc gctacgatcc gctgatcgag cagttgggcg gcaagccgac cgccgcgtgc ggctgggcga tgggtgtcga gcgcatcctc gagctcctga aggaagagca cctcgtgccg gagcaggaag gcgtcgacgt gtacgtcgtc catcagggcg acgcggcgcg cgagcaggcg ttcatcgtcg ccgagcgtct gcgcgacacc ggcctcgacg tgatcctgca ttgcagcgcg gacggcgcgg gcgcgagctt caagtcgcag atgaagcgcg cggatgcaag cggcgcggcg ttcgcggtga tcttcggcga agacgaggtc gcgaacggca cggtgagcgt gaagccgctg cgcggcacgg gcgccgaagg cgagaagaac gttcagcagt ccgtgccggt cgaaagcttg accgaatttc taatcaatgc gatggttgca accgccgaag acggcg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTEQKRKLE KLTGVKGMND ILPQDAGLWE FFEATVKSLL RAYGYQNIRT PIVEHTQLFT RGIGEVTDIV EKEMYSFVDA LNGENLTLRP ENTAAVVRAA IEHNMLYDGP KRLWYLGPMF RHERPQRGRY RQFHQVGVEA LGFAGPDADA EIIMMCQRLW DDLGLTGIKL EINSLGLAEE RAAHRVELIK YLEQHVDKLD DDAQRRLYTN PLRVLDTKNP ALQEIVRNAP QLIDFLGDVS RAHFDGLQQL LKANNLPFTI NPRLVRGLDY YNLTVFEWVT DKLGAQGTVA AGGRYDPLIE QLGGKPTAAC GWAMGVERIL ELLKEEHLVP EQEGVDVYVV HQGDAAREQA FIVAERLRDT GLDVILHCSA DGAGASFKSQ MKRADASGAA FAVIFGEDEV ANGTVSVKPL RGTGAEGEKN VQQSVPVESL TEFLINAMVA TAEDGDD
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACAGAACAA AAGCGAAAGC TCGAGAAGCT GACGGGCGTG AAGGGCATGA ACGACATCCT CCCGCAGGAT GCCGGCTTGT GGGAATTCTT CGAGGCGACG GTGAAGTCGC TGCTGCGCGC ATACGGCTAT CAGAACATCC GCACGCCGAT CGTCGAGCAT ACGCAGCTCT TCACGCGCGG CATCGGCGAG GTGACCGACA TCGTCGAAAA GGAGATGTAC AGCTTCGTCG ATGCGTTGAA CGGCGAGAAC CTGACGCTGC GCCCCGAGAA CACCGCGGCC GTCGTGCGCG CGGCGATCGA GCACAACATG CTGTATGACG GCCCGAAACG CCTGTGGTAT CTCGGGCCGA TGTTCCGCCA CGAGCGCCCG CAGCGCGGCC GTTATCGCCA GTTCCATCAG GTCGGCGTCG AGGCGCTCGG CTTCGCGGGC CCGGACGCGG ACGCGGAAAT CATCATGATG TGCCAGCGCC TGTGGGACGA TCTCGGCCTC ACCGGCATCA AGCTCGAGAT CAACTCGCTC GGTCTCGCCG AGGAGCGCGC CGCGCACCGC GTCGAGCTCA TCAAGTATCT CGAGCAGCAC GTCGACAAGC TCGACGACGA CGCGCAGCGC CGCCTCTACA CCAACCCGCT GCGCGTGCTC GACACGAAGA ATCCGGCGCT GCAGGAGATC GTGCGGAACG CGCCGCAGCT GATCGATTTC CTCGGCGACG TGTCGCGCGC GCACTTCGAC GGCTTGCAGC AGCTGCTGAA GGCGAACAAC CTGCCGTTTA CGATCAATCC GCGGCTCGTG CGCGGGCTCG ACTACTACAA CCTGACCGTG TTCGAGTGGG TGACCGACAA GCTCGGCGCG CAGGGCACGG TCGCCGCGGG CGGCCGCTAC GATCCGCTGA TCGAGCAGTT GGGCGGCAAG CCGACCGCCG CGTGCGGCTG GGCGATGGGT GTCGAGCGCA TCCTCGAGCT CCTGAAGGAA GAGCACCTCG TGCCGGAGCA GGAAGGCGTC GACGTGTACG TCGTCCATCA GGGCGACGCG GCGCGCGAGC AGGCGTTCAT CGTCGCCGAG CGTCTGCGCG ACACCGGCCT CGACGTGATC CTGCATTGCA GCGCGGACGG CGCGGGCGCG AGCTTCAAGT CGCAGATGAA GCGCGCGGAT GCAAGCGGCG CGGCGTTCGC GGTGATCTTC GGCGAAGACG AGGTCGCGAA CGGCACGGTG AGCGTGAAGC CGCTGCGCGG CACGGGCGCC GAAGGCGAGA AGAACGTTCA GCAGTCCGTG CCGGTCGAAA GCTTGACCGA ATTTCTAATC AATGCGATGG TTGCAACCGC CGAAGACGGC GACGACTGAA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A