BupsA.00062.a

inositol-5-monophosphate dehydrogenase

CENTER ID:	BupsA.00062.a
ORGANISM:	Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE:	Melioidosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.00062.a.A1.GE25031	Full length( BupsA.00062.a )		1	486

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.00062.a.A1.PW25954	Full length( BupsA.00062.a )		1	486

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_126887
PATRIC ID:	fig\|320372.6.peg.2674
RefSeq:	YP_333941
UniProt:	Q3JR62

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRLIQKALTF DDVLLVPAFS DVLPRDTSLK TQLTRNISLN MPLVSAAMDT VTEGRLAIAM AQQGGVGIVH KNLTPVEQAR EVAKVKRFES GVVRDPITVP PSMKVRDVIA LSRQHGISGF PVVEGPKLVG IVTNRDLRFE TRLDEPVKSI MTPRERLVTV AEGTPLAEAK ALMHSHRLER VLVVNDAFEL RGLMTVKDIT KQTEHPEACK DEHGKLRVGA AVGVGADNEE RVELLVQAGV DVIVVDTAHG HSKGVLERVR WVKQNFPKVE VIGGNIATAA AAKALVEYGA DAVKVGIGPG SICTTRIVAG VGVPQISAIA NVSDALRGTG VPCVADGGIR FSGDVSKALA AGANAVMMGS MFAGTEEAPG DVFLYQGRQY KSYRGMGSVG AMKDGAADRY FQDNSANIDK LVPEGIEGRV AYKGSVNAII FQLIGGVRAS MGYCGCKTIA ELHEKAEFVQ ITAAGMRESH VHDVQITKEA PNYHVD

NT Sequence

ATGCGTCTGA TCCAAAAAGC ACTCACGTTC GATGACGTGC TCCTCGTCCC GGCGTTCTCC GACGTTCTGC CGCGCGACAC CAGCCTCAAG ACCCAGCTGA CCCGCAACAT CTCCCTGAAC ATGCCGCTCG TGTCCGCCGC GATGGACACG GTCACCGAAG GCCGCCTCGC GATCGCGATG GCGCAGCAAG GCGGCGTCGG CATCGTCCAC AAGAATCTCA CCCCCGTCGA GCAGGCGCGC GAGGTCGCGA AGGTCAAGCG CTTCGAATCG GGCGTCGTGC GCGATCCGAT CACCGTTCCG CCGAGCATGA AGGTGCGCGA CGTGATCGCG CTGTCGCGCC AGCATGGCAT CTCGGGCTTC CCGGTCGTCG AAGGTCCGAA GCTCGTCGGC ATCGTGACGA ACCGCGACCT GCGCTTCGAA ACCCGCCTCG ACGAGCCGGT CAAGTCGATC ATGACGCCGC GCGAGCGCCT CGTGACGGTG GCCGAGGGCA CGCCGCTCGC CGAGGCGAAG GCGCTGATGC ACAGCCACCG TCTCGAGCGC GTGCTCGTCG TCAACGACGC GTTCGAGCTG CGCGGCCTGA TGACGGTCAA GGACATCACG AAGCAGACCG AGCACCCGGA AGCGTGCAAG GACGAGCACG GCAAGCTGCG CGTCGGCGCG GCGGTCGGCG TCGGTGCGGA CAACGAAGAG CGTGTCGAGC TGCTCGTGCA GGCGGGCGTC GACGTGATCG TCGTCGATAC CGCGCACGGC CACAGCAAGG GCGTGCTCGA GCGCGTGCGC TGGGTCAAGC AGAACTTCCC GAAGGTCGAG GTGATCGGCG GCAACATCGC GACCGCGGCT GCCGCGAAGG CACTCGTCGA ATACGGCGCG GACGCGGTGA AGGTCGGCAT CGGCCCGGGC TCGATCTGTA CGACGCGGAT CGTCGCGGGC GTCGGCGTGC CGCAGATCAG CGCGATCGCG AACGTGTCGG ATGCGCTGCG CGGCACCGGC GTGCCGTGCG TCGCCGACGG CGGCATCCGC TTCTCGGGCG ACGTGTCGAA GGCGCTCGCC GCCGGCGCGA ACGCGGTGAT GATGGGCAGC ATGTTCGCCG GCACCGAAGA GGCGCCGGGC GACGTGTTCC TCTACCAGGG CCGCCAGTAC AAGTCGTATC GCGGCATGGG CTCGGTCGGC GCGATGAAGG ACGGCGCGGC GGACCGCTAC TTCCAGGACA ACTCCGCGAA CATCGACAAG CTCGTGCCGG AAGGCATCGA AGGCCGCGTC GCGTACAAGG GCTCGGTCAA CGCGATCATC TTCCAGTTGA TCGGCGGCGT GCGCGCGAGC ATGGGCTACT GCGGCTGCAA GACGATCGCC GAGCTGCACG AGAAGGCCGA GTTCGTTCAG ATCACCGCAG CCGGCATGCG CGAGTCGCAC GTGCACGACG TGCAGATCAC GAAGGAAGCC CCCAACTATC ACGTGGAC

Details for BupsA.00062.a.A1.GE25031

HARVESTED ON:	4/7/2008
SEQUENCED ON:	6/16/2009
EXPECTED MW:	54kDa
OBSERVED MW:	54kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	67
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMRLIQKALT FDDVLLVPAF SDVLPRDTSL KTQLTRNISL NMPLVSAAMD TVTEGRLAIA MAQQGGVGIV HKNLTPVEQA REVAKVKRFE SGVVRDPITV PPSMKVRDVI ALSRQHGISG FPVVEGPKLV GIVTNRDLRF ETRLDEPVKS IMTPRERLVT VAEGTPLAEA KALMHSHRLE RVLVVNDAFE LRGLMTVKDI TKQTEHPEAC KDEHGKLRVG AAVGVGADNE ERVELLVQAG VDVIVVDTAH GHSKGVLERV RWVKQNFPXG RGDRRQHRDR GCREGTRRIR RGRGEGRHRP GLDLYDADRR GRRRAADQRD RERVGCAARH RRAVRRRRRH PLLGRRVEGA RRRRERGDDG QHVRRHRRGA GRRVPLPGPP VQVVSRHGLG RRDEGRRGGP LLPGQLREHR QARAGRHRRP RRVQGLGQRD HLPVDRRRAR EHGLLRLQDD RRAAREGRVR SDHRSRHARV ARARRADHEG SPQLSRG

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgcgtc tgatccaaaa agcactcacg ttcgatgacg tgctcctcgt cccggcgttc tccgacgttc tgccgcgcga caccagcctc aagacccagc tgacccgcaa catctccctg aacatgccgc tcgtgtccgc cgcgatggac acggtcaccg aaggccgcct cgcgatcgcg atggcgcagc aaggcggcgt cggcatcgtc cacaagaatc tcacccccgt cgagcaggcg cgcgaggtcg cgaaggtcaa gcgcttcgaa tcgggcgtcg tgcgcgatcc gatcaccgtt ccgccgagca tgaaggtgcg cgacgtgatc gcgctgtcgc gccagcatgg catctcgggc ttcccggtcg tcgaaggtcc gaagctcgtc ggcatcgtga cgaaccgcga cctgcgcttc gaaacccgcc tcgacgagcc ggtcaagtcg atcatgacgc cgcgcgagcg cctcgtgacg gtggccgagg gcacgccgct cgccgaggcg aaggcgctga tgcacagcca ccgtctcgag cgcgtgctcg tcgtcaacga cgcgttcgag ctgcgcggcc tgatgacggt caaggacatc acgaagcaga ccgagcaccc ggaagcgtgc aaggacgagc acggcaagct gcgcgtcggc gcggcggtcg gcgtcggtgc ggacaacgaa gagcgtgtcg agctgctcgt gcaggcgggc gtcgacgtga tcgtcgtcga taccgcgcac ggccacagca agggcgtgct cgagcgcgtg cgctgggtca agcagaactt cccgaanggt cgaggtgatc ggcggcaaca tcgcgaccgc ggctgccgcg aaggcactcg tcgaatacgg cgcggacgcg gtgaaggtcg gcatcggccc gggctcgatc tgtacgacgc ggatcgtcgc gggcgtcggc gtgccgcaga tcagcgcgat cgcgaacgtg tcggatgcgc tgcgcggcac cggcgtgccg tgcgtcgccg acggcggcat ccgcttctcg ggcgacgtgt cgaaggcgct cgccgccggc gcgaacgcgg tgatgatggg cagcatgttc gccggcaccg aagaggcgcc gggcgacgtg ttcctctacc agggccgcca gtacaagtcg tatcgcggca tgggctcggt cggcgcgatg aaggacggcg cggcggaccg ctacttccag gacaactccg cgaacatcga caagctcgtg ccggaaggca tcgaaggccg cgtcgcgtac aagggctcgg tcaacgcgat catcttccag ttgatcggcg gcgtgcgcgc gagcatgggc tactgcggct gcaagacgat cgccgagctg cacgagaagg ccgagttcgt tcagatcacc gcagccggca tgcgcgagtc gcacgtgcac gacgtgcaga tcacgaagga agcccccaac tatcacgtgg ac

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMRLIQKALT FDDVLLVPAF SDVLPRDTSL KTQLTRNISL NMPLVSAAMD TVTEGRLAIA MAQQGGVGIV HKNLTPVEQA REVAKVKRFE SGVVRDPITV PPSMKVRDVI ALSRQHGISG FPVVEGPKLV GIVTNRDLRF ETRLDEPVKS IMTPRERLVT VAEGTPLAEA KALMHSHRLE RVLVVNDAFE LRGLMTVKDI TKQTEHPEAC KDEHGKLRVG AAVGVGADNE ERVELLVQAG VDVIVVDTAH GHSKGVLERV RWVKQNFPKV EVIGGNIATA AAAKALVEYG ADAVKVGIGP GSICTTRIVA GVGVPQISAI ANVSDALRGT GVPCVADGGI RFSGDVSKAL AAGANAVMMG SMFAGTEEAP GDVFLYQGRQ YKSYRGMGSV GAMKDGAADR YFQDNSANID KLVPEGIEGR VAYKGSVNAI IFQLIGGVRA SMGYCGCKTI AELHEKAEFV QITAAGMRES HVHDVQITKE APNYHVD

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcgtctgatc caaaaagcac tcacgttcga tgacgtgctc ctcgtcccgg cgttctccga cgttctgccg cgcgacacca gcctcaagac ccagctgacc cgcaacatct ccctgaacat gccgctcgtg tccgccgcga tggacacggt caccgaaggc cgcctcgcga tcgcgatggc gcagcaaggc ggcgtcggca tcgtccacaa gaatctcacc cccgtcgagc aggcgcgcga ggtcgcgaag gtcaagcgct tcgaatcggg cgtcgtgcgc gatccgatca ccgttccgcc gagcatgaag gtgcgcgacg tgatcgcgct gtcgcgccag catggcatct cgggcttccc ggtcgtcgaa ggtccgaagc tcgtcggcat cgtgacgaac cgcgacctgc gcttcgaaac ccgcctcgac gagccggtca agtcgatcat gacgccgcgc gagcgcctcg tgacggtggc cgagggcacg ccgctcgccg aggcgaaggc gctgatgcac agccaccgtc tcgagcgcgt gctcgtcgtc aacgacgcgt tcgagctgcg cggcctgatg acggtcaagg acatcacgaa gcagaccgag cacccggaag cgtgcaagga cgagcacggc aagctgcgcg tcggcgcggc ggtcggcgtc ggtgcggaca acgaagagcg tgtcgagctg ctcgtgcagg cgggcgtcga cgtgatcgtc gtcgataccg cgcacggcca cagcaagggc gtgctcgagc gcgtgcgctg ggtcaagcag aacttcccga aggtcgaggt gatcggcggc aacatcgcga ccgcggctgc cgcgaaggca ctcgtcgaat acggcgcgga cgcggtgaag gtcggcatcg gcccgggctc gatctgtacg acgcggatcg tcgcgggcgt cggcgtgccg cagatcagcg cgatcgcgaa cgtgtcggat gcgctgcgcg gcaccggcgt gccgtgcgtc gccgacggcg gcatccgctt ctcgggcgac gtgtcgaagg cgctcgccgc cggcgcgaac gcggtgatga tgggcagcat gttcgccggc accgaagagg cgccgggcga cgtgttcctc taccagggcc gccagtacaa gtcgtatcgc ggcatgggct cggtcggcgc gatgaaggac ggcgcggcgg accgctactt ccaggacaac tccgcgaaca tcgacaagct cgtgccggaa ggcatcgaag gccgcgtcgc gtacaagggc tcggtcaacg cgatcatctt ccagttgatc ggcggcgtgc gcgcgagcat gggctactgc ggctgcaaga cgatcgccga gctgcacgag aaggccgagt tcgttcagat caccgcagcc ggcatgcgcg agtcgcacgt gcacgacgtg cagatcacga aggaagcccc caactatcac gtggactgaa aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a

Details for BupsA.00062.a.A1.PW25954

PURIFICATION DATe:	10/21/2008
CONCENTRATION:	17.78mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	1
VIAL VOLUME:	50µl
PERCENT IDENTITY:	67
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMRLIQKALT FDDVLLVPAF SDVLPRDTSL KTQLTRNISL NMPLVSAAMD TVTEGRLAIA MAQQGGVGIV HKNLTPVEQA REVAKVKRFE SGVVRDPITV PPSMKVRDVI ALSRQHGISG FPVVEGPKLV GIVTNRDLRF ETRLDEPVKS IMTPRERLVT VAEGTPLAEA KALMHSHRLE RVLVVNDAFE LRGLMTVKDI TKQTEHPEAC KDEHGKLRVG AAVGVGADNE ERVELLVQAG VDVIVVDTAH GHSKGVLERV RWVKQNFPKV EVIGGNIATA AAAKALVEYG ADAVKVGIGP GSICTTRIVA GVGVPQISAI ANVSDALRGT GVPCVADGGI RFSGDVSKAL AAGANAVMMG SMFAGTEEAP GDVFLYQGRQ YKSYRGMGSV GAMKDGAADR YFQDNSANID KLVPEGIEGR VAYKGSVNAI IFQLIGGVRA SMGYCGCKTI AELHEKAEFV QITAAGMRES HVHDVQITKE APNYHVD

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCGTCTGATC CAAAAAGCAC TCACGTTCGA TGACGTGCTC CTCGTCCCGG CGTTCTCCGA CGTTCTGCCG CGCGACACCA GCCTCAAGAC CCAGCTGACC CGCAACATCT CCCTGAACAT GCCGCTCGTG TCCGCCGCGA TGGACACGGT CACCGAAGGC CGCCTCGCGA TCGCGATGGC GCAGCAAGGC GGCGTCGGCA TCGTCCACAA GAATCTCACC CCCGTCGAGC AGGCGCGCGA GGTCGCGAAG GTCAAGCGCT TCGAATCGGG CGTCGTGCGC GATCCGATCA CCGTTCCGCC GAGCATGAAG GTGCGCGACG TGATCGCGCT GTCGCGCCAG CATGGCATCT CGGGCTTCCC GGTCGTCGAA GGTCCGAAGC TCGTCGGCAT CGTGACGAAC CGCGACCTGC GCTTCGAAAC CCGCCTCGAC GAGCCGGTCA AGTCGATCAT GACGCCGCGC GAGCGCCTCG TGACGGTGGC CGAGGGCACG CCGCTCGCCG AGGCGAAGGC GCTGATGCAC AGCCACCGTC TCGAGCGCGT GCTCGTCGTC AACGACGCGT TCGAGCTGCG CGGCCTGATG ACGGTCAAGG ACATCACGAA GCAGACCGAG CACCCGGAAG CGTGCAAGGA CGAGCACGGC AAGCTGCGCG TCGGCGCGGC GGTCGGCGTC GGTGCGGACA ACGAAGAGCG TGTCGAGCTG CTCGTGCAGG CGGGCGTCGA CGTGATCGTC GTCGATACCG CGCACGGCCA CAGCAAGGGC GTGCTCGAGC GCGTGCGCTG GGTCAAGCAG AACTTCCCGA AGGTCGAGGT GATCGGCGGC AACATCGCGA CCGCGGCTGC CGCGAAGGCA CTCGTCGAAT ACGGCGCGGA CGCGGTGAAG GTCGGCATCG GCCCGGGCTC GATCTGTACG ACGCGGATCG TCGCGGGCGT CGGCGTGCCG CAGATCAGCG CGATCGCGAA CGTGTCGGAT GCGCTGCGCG GCACCGGCGT GCCGTGCGTC GCCGACGGCG GCATCCGCTT CTCGGGCGAC GTGTCGAAGG CGCTCGCCGC CGGCGCGAAC GCGGTGATGA TGGGCAGCAT GTTCGCCGGC ACCGAAGAGG CGCCGGGCGA CGTGTTCCTC TACCAGGGCC GCCAGTACAA GTCGTATCGC GGCATGGGCT CGGTCGGCGC GATGAAGGAC GGCGCGGCGG ACCGCTACTT CCAGGACAAC TCCGCGAACA TCGACAAGCT CGTGCCGGAA GGCATCGAAG GCCGCGTCGC GTACAAGGGC TCGGTCAACG CGATCATCTT CCAGTTGATC GGCGGCGTGC GCGCGAGCAT GGGCTACTGC GGCTGCAAGA CGATCGCCGA GCTGCACGAG AAGGCCGAGT TCGTTCAGAT CACCGCAGCC GGCATGCGCG AGTCGCACGT GCACGACGTG CAGATCACGA AGGAAGCCCC CAACTATCAC GTGGACTGAA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A