SSGCID: Target Page

BupsA.00058.a

thioredoxin-disulfide reductase

CENTER ID:	BupsA.00058.a
ORGANISM:	Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE:	Melioidosis
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.00058.a.A1.GE25029	Full length( BupsA.00058.a )		1	320

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|320372.6.peg.3208
RefSeq:	YP_334452
UniProt:	Q3JPQ1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSTPKHAKVL ILGSGPAGYT AAVYAARANL SPLLITGIAQ GGQLMTTTDV ENWPADADGV QGPELMQRFL AHAQRFNTEI VFDHIHTAKL HEKPIRLIGD SGEYTCDSLI IATGASAQYL GLQSEEAFMG RGVSACATCD GFFYRGQNVA VVGGGNTAVE EALYLTGIAK KVTVIHRRDK FRAEPILVDR LLEKEKEGAV EIKWDHVLDE VTGDDSGVSG VRIKHVTTGA TEDVAVQGLF IAIGHKPNTD IFKGQLEMKD GYIITNSGLS GNATGTSVPG VFAAGDVQDH IYRQAITSAG TGCMAALDAQ RYLESLHDHK

NT Sequence

ATGTCCACGC CCAAACACGC GAAAGTCCTG ATTCTCGGTT CCGGCCCCGC CGGCTACACG GCGGCCGTCT ACGCGGCCCG CGCGAACCTG TCGCCCCTCC TGATCACGGG CATCGCGCAA GGCGGCCAGC TGATGACGAC GACCGACGTC GAGAATTGGC CGGCCGACGC GGACGGCGTG CAGGGCCCCG AGCTGATGCA GCGCTTTCTC GCGCACGCGC AGCGCTTCAA CACCGAGATC GTGTTCGACC ACATCCACAC GGCCAAGCTG CACGAGAAGC CGATCCGCCT GATCGGCGAC TCGGGCGAAT ACACGTGCGA CTCGCTGATC ATCGCGACGG GCGCGTCCGC GCAATACCTC GGCCTGCAGT CGGAAGAGGC GTTCATGGGC CGCGGCGTGT CGGCGTGCGC GACCTGCGAC GGCTTCTTCT ATCGCGGCCA GAACGTCGCG GTCGTCGGCG GCGGCAACAC GGCCGTCGAG GAAGCGCTCT ATCTGACGGG CATCGCGAAG AAGGTCACGG TGATCCACCG CCGCGACAAG TTCCGCGCGG AGCCGATCCT CGTCGATCGC CTGCTCGAGA AGGAAAAGGA AGGCGCGGTC GAGATCAAGT GGGACCATGT GCTCGACGAG GTGACGGGCG ACGATTCGGG CGTCTCGGGC GTGCGCATCA AGCACGTGAC GACGGGCGCG ACCGAGGACG TCGCGGTGCA GGGCCTGTTC ATCGCGATCG GCCACAAGCC GAACACCGAC ATCTTCAAGG GCCAGCTCGA GATGAAGGAC GGCTACATCA TCACGAACAG CGGCCTGTCG GGCAACGCGA CGGGCACGAG CGTGCCGGGC GTGTTCGCGG CGGGCGACGT GCAGGACCAC ATCTACCGCC AGGCGATCAC GAGCGCGGGC ACGGGCTGCA TGGCGGCGCT CGACGCGCAG CGCTATCTCG AAAGCCTGCA CGACCACAAG

Details for BupsA.00058.a.A1.GE25029

HARVESTED ON:	2/12/2008
SEQUENCED ON:	6/16/2009
EXPECTED MW:	36kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	97
PERCENT COVERAGE:	90

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMSTPKHAKV LILGSGPAGY TAAVYAARAN LSPLLITGIA QGGQLMTTTD VENWPADADG VQGPELMQRF LAHAQRFNTE IVFDHIHTAK LHEKPIRLIG DSGEYTCDSL IIATGASAQY LGLQSEEAFM GRGVSACATC DGFFYRGQNV AVVGGGNTAV EEALYLTGIA KKVTVIHRRD KFRAEPILVD RLLEKEKEGA VEIKWDHVLD EVTGDDSGVS GVRIKHVTTG ATXXVAVXGL FIAIGHKPNT DIFKGQLEMX XXYIITNSGL SGNATGTSVX GVFAAGDV

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgtcca cgcccaaaca cgcgaaagtc ctgattctcg gttccggccc cgccggctac acggcggccg tctacgcggc ccgcgcgaac ctgtcgcccc tcctgatcac gggcatcgcg caaggcggcc agctgatgac gacgaccgac gtcgagaatt ggccggccga cgcggacggc gtgcagggcc ccgagctgat gcagcgcttt ctcgcgcacg cgcagcgctt caacaccgag atcgtgttcg accacatcca cacggccaag ctgcacgaga agccgatccg cctgatcggc gactcgggcg aatacacgtg cgactcgctg atcatcgcga cgggcgcgtc cgcgcaatac ctcggcctgc agtcggaaga ggcgttcatg ggccgcggcg tgtcggcgtg cgcgacctgc gacggcttct tctatcgcgg ccagaacgtc gcggtcgtcg gcggcggcaa cacggccgtc gaggaagcgc tctatctgac gggcatcgcg aagaaggtca cggtgatcca ccgccgcgac aagttccgcg cggagccgat cctcgtcgat cgcctgctcg agaaggaaaa ggaaggcgcg gtcgagatca agtgggacca tgtgctcgac gaggtgacgg gcgacgattc gggcgtctcg ggcgtgcgca tcaagcacgt gacgacgggc gcgaccgang nngtcgcggt gcanggcctg ttcatcgcga tcggccacaa gccgaacacc gacatcttca agggccagct cgagatgaan ganngntaca tcatcacgaa cagcggcctg tcgggcaacg cgacgggcac gagcgtgncg ggcgtgttcg cggcgggcga cgtgc

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMSTPKHAKV LILGSGPAGY TAAVYAARAN LSPLLITGIA QGGQLMTTTD VENWPADADG VQGPELMQRF LAHAQRFNTE IVFDHIHTAK LHEKPIRLIG DSGEYTCDSL IIATGASAQY LGLQSEEAFM GRGVSACATC DGFFYRGQNV AVVGGGNTAV EEALYLTGIA KKVTVIHRRD KFRAEPILVD RLLEKEKEGA VEIKWDHVLD EVTGDDSGVS GVRIKHVTTG ATEDVAVQGL FIAIGHKPNT DIFKGQLEMK DGYIITNSGL SGNATGTSVP GVFAAGDVQD HIYRQAITSA GTGCMAALDA QRYLESLHDH K

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtccacgccc aaacacgcga aagtcctgat tctcggttcc ggccccgccg gctacacggc ggccgtctac gcggcccgcg cgaacctgtc gcccctcctg atcacgggca tcgcgcaagg cggccagctg atgacgacga ccgacgtcga gaattggccg gccgacgcgg acggcgtgca gggccccgag ctgatgcagc gctttctcgc gcacgcgcag cgcttcaaca ccgagatcgt gttcgaccac atccacacgg ccaagctgca cgagaagccg atccgcctga tcggcgactc gggcgaatac acgtgcgact cgctgatcat cgcgacgggc gcgtccgcgc aatacctcgg cctgcagtcg gaagaggcgt tcatgggccg cggcgtgtcg gcgtgcgcga cctgcgacgg cttcttctat cgcggccaga acgtcgcggt cgtcggcggc ggcaacacgg ccgtcgagga agcgctctat ctgacgggca tcgcgaagaa ggtcacggtg atccaccgcc gcgacaagtt ccgcgcggag ccgatcctcg tcgatcgcct gctcgagaag gaaaaggaag gcgcggtcga gatcaagtgg gaccatgtgc tcgacgaggt gacgggcgac gattcgggcg tctcgggcgt gcgcatcaag cacgtgacga cgggcgcgac cgaggacgtc gcggtgcagg gcctgttcat cgcgatcggc cacaagccga acaccgacat cttcaagggc cagctcgaga tgaaggacgg ctacatcatc acgaacagcg gcctgtcggg caacgcgacg ggcacgagcg tgccgggcgt gttcgcggcg ggcgacgtgc aggaccacat ctaccgccag gcgatcacga gcgcgggcac gggctgcatg gcggcgctcg acgcgcagcg ctatctcgaa agcctgcacg accacaagta aaaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa