BupsA.00057.c

ABC transport system ATP-binding protein

CENTER ID: BupsA.00057.c
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:
IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
BupsA.00057.c.A1.GE27511 Full length( BupsA.00057.c ) 1 240
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_215159
PATRIC ID: fig|320372.6.peg.259
RefSeq: YP_331682.1
UniProt: Q3JXM1
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MIEISNLTVQ FGGTRVLDAL DAKLAAPICG LIGPNGAGKT TLLNVLSGFL RPRAGSVALD GRALLALSVA ERVRAGVRRT FQTEQIVEDL SVHDNVLALA EHVMPALAAR DDTRRALELV GLADVAHAQG AALNLYQRRM LELGKALVGA PRLLLLDEPG AGLNETEAAR LADVIVRIPE CVGAQVLLID HDVDLIDAVC EATLVLDFGK RLALGPTRAV LDDPLVRSAY LGQAFDEHEA
NT Sequence
ATGATCGAAA TCTCGAACCT GACCGTGCAA TTCGGCGGCA CGCGCGTGCT CGACGCGCTC GACGCGAAGC TCGCTGCGCC CATATGCGGG CTGATCGGCC CGAACGGCGC GGGCAAGACG ACGCTGCTGA ACGTACTGAG CGGCTTCTTG CGGCCGCGTG CGGGCAGCGT CGCGCTGGAC GGCCGCGCGC TGCTCGCGCT GTCCGTCGCC GAGCGCGTGC GCGCGGGCGT GCGGCGCACG TTCCAGACCG AACAGATCGT CGAGGATCTG AGCGTGCACG ACAACGTGCT CGCGCTCGCC GAGCACGTGA TGCCGGCCCT CGCCGCGCGC GACGACACGC GGCGCGCGCT CGAACTCGTC GGCCTCGCCG ACGTCGCGCA CGCGCAGGGC GCGGCGCTCA ACCTGTATCA GCGCCGCATG CTGGAACTCG GCAAGGCGCT CGTCGGCGCG CCGCGCCTGC TGCTGCTCGA CGAGCCGGGC GCGGGCCTGA ACGAGACCGA AGCCGCGCGG CTCGCCGACG TGATCGTTCG CATTCCCGAA TGCGTCGGCG CGCAGGTGCT GCTGATCGAT CACGACGTCG ACCTGATCGA TGCCGTGTGC GAGGCGACGC TCGTGCTCGA TTTCGGCAAG CGGCTCGCGC TCGGGCCCAC GCGCGCGGTG CTCGACGATC CGCTCGTGCG CAGTGCGTAT CTCGGCCAAG CGTTCGACGA ACATGAAGCG
Details for BupsA.00057.c.A1.GE27511
HARVESTED ON: 7/21/2009
SEQUENCED ON: 7/29/2009
EXPECTED MW: 27kDa
OBSERVED MW: 27kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMIEISNLTV QFGGTRVLDA LDAKLAAPIC GLIGPNGAGK TTLLNVLSGF LRPRAGSVAL DGRALLALSV AERVRAGVRR TFQTEQIVED LSVHDNVLAL AEHVMPALAA RDDTRRALEL VGLADVAHAQ GAALNLYQRR MLELGKALVG APRLLLLDEP GAGLNETEAA RLADVIVRIP ECVGAQVLLI DHDVDLIDAV CEATLVLDFG KRLALGPTRA VLDDPLVRSA YLGQAFDEH
Validated NT Sequence
tttaagaagg agnnatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgatcga aatctcgaac ctgaccgtgc aattcggcgg cacgcgcgtg ctcgacgcgc tcgacgcgaa gctcgctgcg cccatatgcg ggctgatcgg cccgaacggc gcgggcaaga cgacgctgct gaacgtactg agcggcttct tgcggccgcg tgcgggcagc gtcgcgctgg acggccgcgc gctgctcgcg ctgtccgtcg ccgagcgcgt gcgcgcgggc gtgcggcgca cgttccagac cgaacagatc gtcgaggatc tgagcgtgca cgacaacgtg ctcgcgctcg ccgagcacgt gatgccggcc ctcgccgcgc gcgacgacac gcggcgcgcg ctcgaactcg tcggcctcgc cgacgtcgcg cacgcgcagg gcgcggcgct caacctgtat cagcgccgca tgctggaact cggcaaggcg ctcgtcggcg cgccgcgcct gctgctgctc gacgagccgg gcgcgggcct gaacgagacc gaagccgcgc ggctcgccga cgtgatcgtt cgcattcccg aatgcgtcgg cgcgcaggtg ctgctgatcg atcacgacgt cgacctgatc gatgccgtgt gcgaggcgac gctcgtgctc gatttcggca agcggctcgc gctcgggccc acgcgcgcgg tgctcgacga tccgctcgtg cgcagtgcgt atctcggcca agcgttcgac gaacatg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMIEISNLTV QFGGTRVLDA LDAKLAAPIC GLIGPNGAGK TTLLNVLSGF LRPRAGSVAL DGRALLALSV AERVRAGVRR TFQTEQIVED LSVHDNVLAL AEHVMPALAA RDDTRRALEL VGLADVAHAQ GAALNLYQRR MLELGKALVG APRLLLLDEP GAGLNETEAA RLADVIVRIP ECVGAQVLLI DHDVDLIDAV CEATLVLDFG KRLALGPTRA VLDDPLVRSA YLGQAFDEHE A
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gatcgaaatc tcgaacctga ccgtgcaatt cggcggcacg cgcgtgctcg acgcgctcga cgcgaagctc gctgcgccca tatgcgggct gatcggcccg aacggcgcgg gcaagacgac gctgctgaac gtactgagcg gcttcttgcg gccgcgtgcg ggcagcgtcg cgctggacgg ccgcgcgctg ctcgcgctgt ccgtcgccga gcgcgtgcgc gcgggcgtgc ggcgcacgtt ccagaccgaa cagatcgtcg aggatctgag cgtgcacgac aacgtgctcg cgctcgccga gcacgtgatg ccggccctcg ccgcgcgcga cgacacgcgg cgcgcgctcg aactcgtcgg cctcgccgac gtcgcgcacg cgcagggcgc ggcgctcaac ctgtatcagc gccgcatgct ggaactcggc aaggcgctcg tcggcgcgcc gcgcctgctg ctgctcgacg agccgggcgc gggcctgaac gagaccgaag ccgcgcggct cgccgacgtg atcgttcgca ttcccgaatg cgtcggcgcg caggtgctgc tgatcgatca cgacgtcgac ctgatcgatg ccgtgtgcga ggcgacgctc gtgctcgatt tcggcaagcg gctcgcgctc gggcccacgc gcgcggtgct cgacgatccg ctcgtgcgca gtgcgtatct cggccaagcg ttcgacgaac atgaagcgaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa