BupsA.00056.a
nicotinate-nucleotide pyrophosphorylase
| CENTER ID: | BupsA.00056.a |
| ORGANISM: | Burkholderia pseudomallei 1710b |
| ASSOCIATED DISEASE: | Melioidosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BupsA.00056.a.A1.GE25027 | Full length( BupsA.00056.a ) | 1 | 294 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
3GNN
DEPOSITED: 3/17/2009
DETERMINATION: XRay
CLONE: BupsA.00056.a.A1.GE25027
PROTEIN: BupsA.00056.a.A1.PW25065
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_128545 |
| PATRIC ID: | fig|320372.6.peg.1146 |
| RefSeq: | YP_332544 |
| UniProt: | Q3JV59 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTIDAVSPLF ADISREYGAA FDAAIARNVA DALAEDVGSG DQTGRLVPDG APRRARVIVR EDAVLCGVPW FDAVVRAVDP SIEVDWRHRE GDRMSADSTV CELRGPARAL LTAERNALNF LQLLSGVASA TRQYVDRIAD TRARILDTRK TLPGLRLAQK YAVRVGGGAN QRLALYAGIL IKENHIAAAG GVGEALDAAF ALNAEVPVQI EVETLDQLRT ALAHGARSVL LDNFTLDMMR DAVRVTEGRA VLEVSGGVNF DTVRAIAETG VDRISIGALT KDVRATDYSM RIVE
NT Sequence
ATGACGATCG ACGCGGTATC GCCGCTCTTT GCCGACATCT CGCGCGAATA CGGCGCCGCG TTCGACGCGG CGATCGCGCG CAACGTCGCC GATGCGCTCG CGGAGGATGT CGGCAGCGGC GACCAGACCG GGCGCCTCGT GCCGGACGGC GCGCCGCGCC GCGCGCGCGT GATCGTGCGC GAGGACGCGG TGCTGTGCGG CGTGCCGTGG TTCGACGCGG TGGTGCGCGC GGTCGATCCG TCGATCGAAG TCGACTGGCG CCATCGCGAA GGCGACCGGA TGAGCGCGGA TTCGACCGTC TGCGAACTGC GCGGCCCCGC GCGCGCGCTG CTGACGGCCG AACGCAACGC GCTGAACTTC CTGCAACTGC TGTCGGGCGT CGCGAGCGCG ACGCGGCAGT ATGTCGACCG GATCGCCGAT ACGCGGGCGC GGATCCTCGA TACGCGCAAG ACGCTGCCGG GCCTGCGGCT CGCCCAGAAG TACGCGGTGC GCGTCGGCGG CGGCGCGAAC CAGCGGCTGG CGCTCTACGC GGGCATCCTG ATCAAGGAAA ATCACATCGC GGCGGCGGGC GGGGTGGGCG AGGCGCTCGA CGCGGCGTTC GCGCTGAACG CCGAAGTGCC GGTGCAGATC GAGGTCGAAA CGCTCGACCA GTTGCGCACG GCGCTCGCGC ACGGCGCGCG ATCGGTGCTG CTCGATAACT TCACGCTCGA CATGATGCGC GATGCGGTGC GTGTCACCGA GGGCCGTGCG GTGCTCGAGG TGTCGGGCGG CGTGAATTTC GATACGGTGC GCGCGATCGC GGAGACGGGC GTCGATCGGA TCTCGATCGG TGCGCTGACG AAGGACGTGC GCGCGACCGA TTATTCGATG CGTATCGTCG AA
Details for BupsA.00056.a.A1.GE25027
| HARVESTED ON: | 3/31/2008 |
| SEQUENCED ON: | 7/29/2008 |
| EXPECTED MW: | 33kDa |
| OBSERVED MW: | 34kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTIDAVSPL FADISREYGA AFDAAIARNV ADALAEDVGS GDQTGRLVPD GAPRRARVIV REDAVLCGVP WFDAVVRAVD PSIEVDWRHR EGDRMSADST VCELRGPARA LLTAERNALN FLQLLSGVAS ATRQYVDRIA DTRARILDTR KTLPGLRLAQ KYAVRVGGGA NQRLALYAGI LIKENHIAAA GGVGEALDAA FALNAEVPVQ IEVETLDQLR TALAHGARSV LLDNFTLDMM RDAVRVTEGR AVLEVSGGVN FDTVRAIAET GVDRISIGAL TKDVRATDYS MRIVE
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgacga tcgacgcggt atcgccgctc tttgccgaca tctcgcgcga atacggcgcc gcgttcgacg cggcgatcgc gcgcaacgtc gccgatgcgc tcgcggagga tgtcggcagc ggcgaccaga ccgggcgcct cgtgccggac ggcgcgccgc gccgcgcgcg cgtgatcgtg cgcgaggacg cggtgctgtg cggcgtgccg tggttcgacg cggtggtgcg cgcggtcgat ccgtcgatcg aagtcgactg gcgccatcgc gaaggcgacc ggatgagcgc ggattcgacc gtctgcgaac tgcgcggccc cgcgcgcgcg ctgctgacgg ccgaacgcaa cgcgctgaac ttcctgcaac tgctgtcggg cgtcgcgagc gcgacgcggc agtatgtcga ccggatcgcc gatacgcggg cgcggatcct cgatacgcgc aagacgctgc cgggcctgcg gctcgcccag aagtacgcgg tgcgcgtcgg cggcggcgcg aaccagcggc tggcgctcta cgcgggcatc ctgatcaagg aaaatcacat cgcggcggcg ggcggggtgg gcgaggcgct cgacgcggcg ttcgcgctga acgccgaagt gccggtgcag atcgaggtcg aaacgctcga ccagttgcgc acggcgctcg cgcacggcgc gcgatcggtg ctgctcgata acttcacgct cgacatgatg cgcgatgcgg tgcgtgtcac cgagggccgt gcggtgctcg aggtgtcggg cggcgtgaat ttcgatacgg tgcgcgcgat cgcggagacg ggcgtcgatc ggatctcgat cggtgcgctg acgaaggacg tgcgcgcgac cgattattcg atgcgtatcg tcgaataata a
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTIDAVSPL FADISREYGA AFDAAIARNV ADALAEDVGS GDQTGRLVPD GAPRRARVIV REDAVLCGVP WFDAVVRAVD PSIEVDWRHR EGDRMSADST VCELRGPARA LLTAERNALN FLQLLSGVAS ATRQYVDRIA DTRARILDTR KTLPGLRLAQ KYAVRVGGGA NQRLALYAGI LIKENHIAAA GGVGEALDAA FALNAEVPVQ IEVETLDQLR TALAHGARSV LLDNFTLDMM RDAVRVTEGR AVLEVSGGVN FDTVRAIAET GVDRISIGAL TKDVRATDYS MRIVE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacgatcgac gcggtatcgc cgctctttgc cgacatctcg cgcgaatacg gcgccgcgtt cgacgcggcg atcgcgcgca acgtcgccga tgcgctcgcg gaggatgtcg gcagcggcga ccagaccggg cgcctcgtgc cggacggcgc gccgcgccgc gcgcgcgtga tcgtgcgcga ggacgcggtg ctgtgcggcg tgccgtggtt cgacgcggtg gtgcgcgcgg tcgatccgtc gatcgaagtc gactggcgcc atcgcgaagg cgaccggatg agcgcggatt cgaccgtctg cgaactgcgc ggccccgcgc gcgcgctgct gacggccgaa cgcaacgcgc tgaacttcct gcaactgctg tcgggcgtcg cgagcgcgac gcggcagtat gtcgaccgga tcgccgatac gcgggcgcgg atcctcgata cgcgcaagac gctgccgggc ctgcggctcg cccagaagta cgcggtgcgc gtcggcggcg gcgcgaacca gcggctggcg ctctacgcgg gcatcctgat caaggaaaat cacatcgcgg cggcgggcgg ggtgggcgag gcgctcgacg cggcgttcgc gctgaacgcc gaagtgccgg tgcagatcga ggtcgaaacg ctcgaccagt tgcgcacggc gctcgcgcac ggcgcgcgat cggtgctgct cgataacttc acgctcgaca tgatgcgcga tgcggtgcgt gtcaccgagg gccgtgcggt gctcgaggtg tcgggcggcg tgaatttcga tacggtgcgc gcgatcgcgg agacgggcgt cgatcggatc tcgatcggtg cgctgacgaa ggacgtgcgc gcgaccgatt attcgatgcg tatcgtcgaa tgaaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa