BupsA.00054.a

tRNA (guanine-N(1)-)-methyltransferase

CENTER ID: BupsA.00054.a
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.00054.a.A1.GE25025 Full length( BupsA.00054.a ) 1 264
BupsA.00054.a.A1.GE25025 Full length( BupsA.00054.a ) 1 264
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|320372.6.peg.3097
RefSeq: YP_334345
UniProt: Q3JQ08

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDEATQSAIQ FDVVTLFPEM FRALTDWGIT SRAVKQGRFG LRTWNPRDFT TDNYRTVDDR PYGGGPGMVM LAKPLEAAIG AAKAAQAAQG VATSRVVMMS PQGAPLTHER VARMAAEPGV VLLCGRYEAI DQRLIDRCVD EELSLGDFVL SGGELPAMAL MDAVVRLLPG VLNDAQSAVQ DSFADGLLDC PHYTRPEEYE GVRVPDVLLG GHHAEIERWR RQEALRNTIA KRPDLIARAR REKLLSRADE AWLASLAKEA KQAS
NT Sequence
ATGGACGAAG CCACGCAGAG CGCTATCCAG TTCGACGTCG TCACACTCTT TCCGGAGATG TTTCGCGCGT TGACCGACTG GGGCATCACG AGCCGCGCGG TGAAGCAGGG GCGGTTCGGC TTGCGCACGT GGAATCCGCG CGATTTCACG ACCGACAACT ACCGCACCGT CGACGATCGC CCGTACGGCG GCGGCCCCGG CATGGTGATG CTCGCGAAAC CGCTCGAGGC GGCGATCGGC GCGGCGAAGG CCGCGCAGGC GGCGCAAGGC GTCGCGACGT CGCGCGTCGT GATGATGTCG CCGCAGGGCG CGCCGCTCAC GCACGAGCGC GTCGCGCGGA TGGCGGCGGA GCCCGGCGTC GTGCTGCTGT GCGGACGTTA CGAGGCGATC GATCAACGGT TGATCGACCG TTGTGTCGAC GAGGAACTGA GCCTCGGCGA TTTCGTGCTG TCGGGCGGCG AATTGCCGGC GATGGCGCTG ATGGACGCCG TCGTGCGACT GCTGCCCGGT GTGCTGAATG ATGCGCAATC GGCGGTGCAG GACAGCTTCG CCGATGGGTT GCTCGATTGC CCGCATTACA CGCGGCCCGA GGAGTACGAG GGCGTGCGCG TGCCGGACGT GCTGCTCGGC GGGCATCATG CGGAGATCGA GCGCTGGCGT CGGCAGGAAG CGTTGAGAAA CACCATCGCG AAGCGCCCCG ACCTGATCGC GCGGGCGCGT CGCGAAAAGT TGCTGAGCCG GGCCGACGAG GCATGGCTTG CAAGTCTCGC GAAAGAAGCG AAGCAGGCCT CC
Details for BupsA.00054.a.A1.GE25025
HARVESTED ON: 3/31/2008
SEQUENCED ON: 7/29/2008
EXPECTED MW: 31kDa
OBSERVED MW: 32kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: failed sequencing
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMDEATQSAI QFDVVTLFPE MFRALTDWGI TSRAVKQGRF GLRTWNPRDF TTDNYRTVDD RPYGGGPGMV MLAKPLEAAI GAAKAAQAAQ GVATSRVVMM SPQGAPLTHE RVARMAAEPG VVLLCGRYEA IDQRLIDRCV DEELSLGDFV LSGGELPAMA LMDAVVRLLP GVLNDAQSAV QDSFADGLLD CPHYTRPEEY EGVRVPDVLL GGHHAEIERW RRQEALRNTI AKRPDLIARA RREKLLSRAD EAWLASLAKE AKQAS
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggacg aagccacgca gagcgctatc cagttcgacg tcgtcacact ctttccggag atgtttcgcg cgttgaccga ctggggcatc acgagccgcg cggtgaagca ggggcggttc ggcttgcgca cgtggaatcc gcgcgatttc acgaccgaca actaccgcac cgtcgacgat cgcccgtacg gcggcggccc cggcatggtg atgctcgcga aaccgctcga ggcggcgatc ggcgcggcga aggccgcgca ggcggcgcaa ggcgtcgcga cgtcgcgcgt cgtgatgatg tcgccgcagg gcgcgccgct cacgcacgag cgcgtcgcgc ggatggcggc ggagcccggc gtcgtgctgc tgtgcggacg ttacgaggcg atcgatcaac ggttgatcga ccgttgtgtc gacgaggaac tgagcctcgg cgatttcgtg ctgtcgggcg gcgaattgcc ggcgatggcg ctgatggacg ccgtcgtgcg actgctgccc ggtgtgctga atgatgcgca atcggcggtg caggacagct tcgccgatgg gttgctcgat tgcccgcatt acacgcggcc cgaggagtac gagggcgtgc gcgtgccgga cgtgctgctc ggcgggcatc atgcggagat cgagcgctgg cgtcggcagg aagcgttgag aaacaccatc gcgaagcgcc ccgacctgat cgcgcgggcg cgtcgcgaaa agttgctgag ccgggccgac gaggcatggc ttgcaagtct cgcgaaagaa gcgaagcagg cctcctaata a
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDEATQSAI QFDVVTLFPE MFRALTDWGI TSRAVKQGRF GLRTWNPRDF TTDNYRTVDD RPYGGGPGMV MLAKPLEAAI GAAKAAQAAQ GVATSRVVMM SPQGAPLTHE RVARMAAEPG VVLLCGRYEA IDQRLIDRCV DEELSLGDFV LSGGELPAMA LMDAVVRLLP GVLNDAQSAV QDSFADGLLD CPHYTRPEEY EGVRVPDVLL GGHHAEIERW RRQEALRNTI AKRPDLIARA RREKLLSRAD EAWLASLAKE AKQAS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggacgaagcc acgcagagcg ctatccagtt cgacgtcgtc acactctttc cggagatgtt tcgcgcgttg accgactggg gcatcacgag ccgcgcggtg aagcaggggc ggttcggctt gcgcacgtgg aatccgcgcg atttcacgac cgacaactac cgcaccgtcg acgatcgccc gtacggcggc ggccccggca tggtgatgct cgcgaaaccg ctcgaggcgg cgatcggcgc ggcgaaggcc gcgcaggcgg cgcaaggcgt cgcgacgtcg cgcgtcgtga tgatgtcgcc gcagggcgcg ccgctcacgc acgagcgcgt cgcgcggatg gcggcggagc ccggcgtcgt gctgctgtgc ggacgttacg aggcgatcga tcaacggttg atcgaccgtt gtgtcgacga ggaactgagc ctcggcgatt tcgtgctgtc gggcggcgaa ttgccggcga tggcgctgat ggacgccgtc gtgcgactgc tgcccggtgt gctgaatgat gcgcaatcgg cggtgcagga cagcttcgcc gatgggttgc tcgattgccc gcattacacg cggcccgagg agtacgaggg cgtgcgcgtg ccggacgtgc tgctcggcgg gcatcatgcg gagatcgagc gctggcgtcg gcaggaagcg ttgagaaaca ccatcgcgaa gcgccccgac ctgatcgcgc gggcgcgtcg cgaaaagttg ctgagccggg ccgacgaggc atggcttgca agtctcgcga aagaagcgaa gcaggcctcc tgaaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for BupsA.00054.a.A1.GE25025
HARVESTED ON: 3/31/2008
SEQUENCED ON: 7/29/2008
EXPECTED MW: 31kDa
OBSERVED MW: 32kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMDEATQSAI QFDVVTLFPE MFRALTDWGI TSRAVKQGRF GLRTWNPRDF TTDNYRTVDD RPYGGGPGMV MLAKPLEAAI GAAKAAQAAQ GVATSRVVMM SPQGAPLTHE RVARMAAEPG VVLLCGRYEA IDQRLIDRCV DEELSLGDFV LSGGELPAMA LMDAVVRLLP GVLNDAQSAV QDSFADGLLD CPHYTRPEEY EGVRVPDVLL GGHHAEIERW RRQEALRNTI AKRPDLIARA RREKLLSRAD EAWLASLAKE AKQAS
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggacg aagccacgca gagcgctatc cagttcgacg tcgtcacact ctttccggag atgtttcgcg cgttgaccga ctggggcatc acgagccgcg cggtgaagca ggggcggttc ggcttgcgca cgtggaatcc gcgcgatttc acgaccgaca actaccgcac cgtcgacgat cgcccgtacg gcggcggccc cggcatggtg atgctcgcga aaccgctcga ggcggcgatc ggcgcggcga aggccgcgca ggcggcgcaa ggcgtcgcga cgtcgcgcgt cgtgatgatg tcgccgcagg gcgcgccgct cacgcacgag cgcgtcgcgc ggatggcggc ggagcccggc gtcgtgctgc tgtgcggacg ttacgaggcg atcgatcaac ggttgatcga ccgttgtgtc gacgaggaac tgagcctcgg cgatttcgtg ctgtcgggcg gcgaattgcc ggcgatggcg ctgatggacg ccgtcgtgcg actgctgccc ggtgtgctga atgatgcgca atcggcggtg caggacagct tcgccgatgg gttgctcgat tgcccgcatt acacgcggcc cgaggagtac gagggcgtgc gcgtgccgga cgtgctgctc ggcgggcatc atgcggagat cgagcgctgg cgtcggcagg aagcgttgag aaacaccatc gcgaagcgcc ccgacctgat cgcgcgggcg cgtcgcgaaa agttgctgag ccgggccgac gaggcatggc ttgcaagtct cgcgaaagaa gcgaagcagg cctcctaata a
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDEATQSAI QFDVVTLFPE MFRALTDWGI TSRAVKQGRF GLRTWNPRDF TTDNYRTVDD RPYGGGPGMV MLAKPLEAAI GAAKAAQAAQ GVATSRVVMM SPQGAPLTHE RVARMAAEPG VVLLCGRYEA IDQRLIDRCV DEELSLGDFV LSGGELPAMA LMDAVVRLLP GVLNDAQSAV QDSFADGLLD CPHYTRPEEY EGVRVPDVLL GGHHAEIERW RRQEALRNTI AKRPDLIARA RREKLLSRAD EAWLASLAKE AKQAS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggacgaagcc acgcagagcg ctatccagtt cgacgtcgtc acactctttc cggagatgtt tcgcgcgttg accgactggg gcatcacgag ccgcgcggtg aagcaggggc ggttcggctt gcgcacgtgg aatccgcgcg atttcacgac cgacaactac cgcaccgtcg acgatcgccc gtacggcggc ggccccggca tggtgatgct cgcgaaaccg ctcgaggcgg cgatcggcgc ggcgaaggcc gcgcaggcgg cgcaaggcgt cgcgacgtcg cgcgtcgtga tgatgtcgcc gcagggcgcg ccgctcacgc acgagcgcgt cgcgcggatg gcggcggagc ccggcgtcgt gctgctgtgc ggacgttacg aggcgatcga tcaacggttg atcgaccgtt gtgtcgacga ggaactgagc ctcggcgatt tcgtgctgtc gggcggcgaa ttgccggcga tggcgctgat ggacgccgtc gtgcgactgc tgcccggtgt gctgaatgat gcgcaatcgg cggtgcagga cagcttcgcc gatgggttgc tcgattgccc gcattacacg cggcccgagg agtacgaggg cgtgcgcgtg ccggacgtgc tgctcggcgg gcatcatgcg gagatcgagc gctggcgtcg gcaggaagcg ttgagaaaca ccatcgcgaa gcgccccgac ctgatcgcgc gggcgcgtcg cgaaaagttg ctgagccggg ccgacgaggc atggcttgca agtctcgcga aagaagcgaa gcaggcctcc tgaaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa