BupsA.00053.a

ATP-dependent protease ATP-binding subunit

CENTER ID: BupsA.00053.a
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: diffraction
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

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Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.00053.a.A1.PS00004 Full length( BupsA.00053.a ) 1 447
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_129971
PATRIC ID: fig|320372.6.peg.388
RefSeq: YP_331799
UniProt: Q3JXA4
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSTMTPAEIV SELDKHIIGQ AKAKKAVAVA LRNRWRRQQV AEPLRQEITP KNILMIGPTG VGKTEIARRL AKLADAPFIK IEATKFTEVG YVGRDVDSIV RDLIEISVKQ TRETEMRKVR SKATDLAEDR ILDVLLPQPR AVGFGASAEH ANDDNNATRQ TFRKRLREGQ LDDKEIELDI EQPAVGMDIM APPGMEEMTE QIRSMFSNLG SGKKQRRKVK IKEALKLLTD EEAAKMLNDE EVKTKAVQNV EQNGIVFLDE IDKITSRNHE GGGGEVSRQG VQRDLLPLVE GTTINTKYGM VKTDHILFIA SGAFHLAKPS DLIPELQGRF PIRVELDSLS VKDFEAILVA TDASLVKQYQ ALLATEDVKL EFADDGIRRL AEIAYAVNEK TENIGARRLY TVIEKLLEEV SFAAGNHAGQ SVTIDSAYVD HALGEVSKDE DLSRYVL
NT Sequence
GTGAGCACCA TGACCCCTGC CGAGATCGTC TCGGAACTCG ACAAGCACAT CATCGGCCAG GCCAAGGCGA AGAAGGCCGT CGCGGTCGCG CTGCGCAACC GCTGGCGCCG CCAGCAGGTG GCCGAGCCGC TGCGCCAGGA AATCACGCCG AAGAACATCC TGATGATCGG GCCGACGGGC GTCGGCAAGA CCGAGATCGC GCGGCGCCTC GCGAAGCTCG CGGACGCGCC GTTCATCAAG ATCGAGGCGA CCAAGTTCAC CGAAGTCGGC TACGTCGGCC GCGACGTCGA CAGCATCGTG CGCGACCTGA TCGAGATCTC GGTCAAGCAG ACCCGTGAAA CGGAGATGCG CAAGGTGCGC AGCAAGGCGA CCGATCTCGC CGAGGATCGC ATCCTCGACG TGCTGCTGCC GCAGCCGCGC GCGGTGGGTT TCGGCGCGAG CGCCGAGCAC GCGAACGACG ACAACAACGC GACGCGCCAG ACGTTTCGCA AGCGCCTGCG CGAAGGCCAG CTCGACGACA AGGAAATCGA GCTCGACATC GAGCAGCCGG CGGTCGGCAT GGACATCATG GCGCCGCCCG GCATGGAAGA GATGACCGAG CAGATTCGCT CGATGTTCTC GAACCTGGGC AGCGGCAAGA AGCAGCGCCG CAAGGTGAAG ATCAAGGAAG CGCTGAAGCT GCTGACCGAC GAGGAAGCGG CGAAGATGCT CAACGACGAG GAAGTGAAGA CGAAGGCCGT GCAGAACGTC GAGCAGAACG GCATCGTGTT CCTCGACGAG ATCGACAAGA TCACGTCGCG CAACCACGAA GGCGGCGGCG GCGAGGTGTC GAGGCAGGGC GTGCAGCGCG ATCTGCTGCC ACTCGTCGAA GGCACGACGA TCAACACGAA GTACGGGATG GTGAAGACCG ATCACATCCT GTTCATCGCG AGCGGCGCGT TCCATCTCGC GAAGCCGAGC GACCTGATTC CCGAGCTGCA AGGGCGCTTT CCGATCCGCG TCGAGCTCGA TTCGCTGTCG GTGAAGGACT TCGAAGCGAT TCTCGTCGCG ACCGACGCGA GCCTCGTCAA GCAGTATCAG GCGCTCCTCG CGACGGAGGA CGTCAAGCTC GAATTCGCCG ACGACGGCAT TCGCCGGCTC GCCGAGATCG CATACGCGGT CAACGAGAAG ACCGAGAACA TCGGCGCGCG GCGCCTGTAC ACGGTGATCG AGAAGCTGCT CGAGGAAGTG TCGTTCGCCG CGGGCAATCA TGCCGGCCAG AGCGTGACGA TCGATTCGGC CTATGTCGAT CACGCGCTCG GCGAAGTGTC GAAGGACGAG GATCTGTCGC GCTACGTGCT G
Details for BupsA.00053.a.A1.PS00004
PURIFICATION DATe: 4/22/2008
CONCENTRATION: 53.9mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 26
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMVSTMTP AEIVSELDKH IIGQAKAKKA VAVALRNRWR RQQVAEPLRQ EITPKNILMI GPTGVGKTEI ARRLAKLADA PFIKIEATKF TEVGYVGRDV DSIVRDLIEI SVKQTRETEM RKVRSKATDL AEDRILDVLL PQPRAVGFGA SAEHANDDNN ATRQTFRKRL REGQLDDKEI ELDIEQPAVG MDIMAPPGME EMTEQIRSMF SNLGSGKKQR RKVKIKEALK LLTDEEAAKM LNDEEVKTKA VQNVEQNGIV FLDEIDKITS RNHEGGGGEV SRQGVQRDLL PLVEGTTINT KYGMVKTDHI LFIASGAFHL AKPSDLIPEL QGRFPIRVEL DSLSVKDFEA ILVATDASLV KQYQALLATE DVKLEFADDG IRRLAEIAYA VNEKTENIGA RRLYTVIEKL LEEVSFAAGN HAGQSVTIDS AYVDHALGEV SKDEDLSRYV
Validated NT Sequence
catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatggtgagc accatgaccc ctgccgagat cgtctcggaa ctcgacaagc acatcatcgg ccaggccaag gcgaagaagg ccgtcgcggt cgcgctgcgc aaccgctggc gccgccagca ggtggccgag ccgctgcgcc aggaaatcac gccgaagaac atcctgatga tcgggccgac gggcgtcggc aagaccgaga tcgcgcggcg cctcgcgaag ctcgcggacg cgccgttcat caagatcgag gcgaccaagt tcaccgaagt cggctacgtc ggccgcgacg tcgacagcat cgtgcgcgac ctgatcgaga tctcggtcaa gcagacccgt gaaacggaga tgcgcaaggt gcgcagcaag gcgaccgatc tcgccgagga tcgcatcctc gacgtgctgc tgccgcagcc gcgcgcggtg ggtttcggcg cgagcgccga gcacgcgaac gacgacaaca acgcgacgcg ccagacgttt cgcaagcgcc tgcgcgaagg ccagctcgac gacaaggaaa tcgagctcga catcgagcag ccggcggtcg gcatggacat catggcgccg cccggcatgg aagagatgac cgagcagatt cgctcgatgt tctcgaacct gggcagcggc aagaagcagc gccgcaaggt gaagatcaag gaagcgctga agctgctgac cgacgaggaa gcggcgaaga tgctcaacga cgaggaagtg aagacgaagg ccgtgcagaa cgtcgagcag aacggcatcg tgttcctcga cgagatcgac aagatcacgt cgcgcaacca cgaaggcggc ggcggcgagg tgtcgaggca gggcgtgcag cgcgatctgc tgccactcgt cgaaggcacg acgatcaaca cgaagtacgg gatggtgaag accgatcaca tcctgttcat cgcgagcggc gcgttccatc tcgcgaagcc gagcgacctg attcccgagc tgcaagggcg ctttccgatc cgcgtcgagc tcgattcgct gtcggtgaag gacttcgaag cgattctcgt cgcgaccgac gcgagcctcg tcaagcagta tcaggcgctc ctcgcgacgg aggacgtcaa gctcgaattc gccgacgacg gcattcgccg gctcgccgag atcgcatacg cggtcaacga gaagaccgag aacatcggcg cgcggcgcct gtacacggtg atcgagaagc tgctcgagga agtgtcgttc gccgcgggca atcatgccgg ccagagcgtg acgatcgatt cggcctatgt cgatcacgcg ctcggcgaag tgtcgaagga cgaggatctg tcgcgctacg tgct
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVSTMTPAE IVSELDKHII GQAKAKKAVA VALRNRWRRQ QVAEPLRQEI TPKNILMIGP TGVGKTEIAR RLAKLADAPF IKIEATKFTE VGYVGRDVDS IVRDLIEISV KQTRETEMRK VRSKATDLAE DRILDVLLPQ PRAVGFGASA EHANDDNNAT RQTFRKRLRE GQLDDKEIEL DIEQPAVGMD IMAPPGMEEM TEQIRSMFSN LGSGKKQRRK VKIKEALKLL TDEEAAKMLN DEEVKTKAVQ NVEQNGIVFL DEIDKITSRN HEGGGGEVSR QGVQRDLLPL VEGTTINTKY GMVKTDHILF IASGAFHLAK PSDLIPELQG RFPIRVELDS LSVKDFEAIL VATDASLVKQ YQALLATEDV KLEFADDGIR RLAEIAYAVN EKTENIGARR LYTVIEKLLE EVSFAAGNHA GQSVTIDSAY VDHALGEVSK DEDLSRYVL
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGAGCACC ATGACCCCTG CCGAGATCGT CTCGGAACTC GACAAGCACA TCATCGGCCA GGCCAAGGCG AAGAAGGCCG TCGCGGTCGC GCTGCGCAAC CGCTGGCGCC GCCAGCAGGT GGCCGAGCCG CTGCGCCAGG AAATCACGCC GAAGAACATC CTGATGATCG GGCCGACGGG CGTCGGCAAG ACCGAGATCG CGCGGCGCCT CGCGAAGCTC GCGGACGCGC CGTTCATCAA GATCGAGGCG ACCAAGTTCA CCGAAGTCGG CTACGTCGGC CGCGACGTCG ACAGCATCGT GCGCGACCTG ATCGAGATCT CGGTCAAGCA GACCCGTGAA ACGGAGATGC GCAAGGTGCG CAGCAAGGCG ACCGATCTCG CCGAGGATCG CATCCTCGAC GTGCTGCTGC CGCAGCCGCG CGCGGTGGGT TTCGGCGCGA GCGCCGAGCA CGCGAACGAC GACAACAACG CGACGCGCCA GACGTTTCGC AAGCGCCTGC GCGAAGGCCA GCTCGACGAC AAGGAAATCG AGCTCGACAT CGAGCAGCCG GCGGTCGGCA TGGACATCAT GGCGCCGCCC GGCATGGAAG AGATGACCGA GCAGATTCGC TCGATGTTCT CGAACCTGGG CAGCGGCAAG AAGCAGCGCC GCAAGGTGAA GATCAAGGAA GCGCTGAAGC TGCTGACCGA CGAGGAAGCG GCGAAGATGC TCAACGACGA GGAAGTGAAG ACGAAGGCCG TGCAGAACGT CGAGCAGAAC GGCATCGTGT TCCTCGACGA GATCGACAAG ATCACGTCGC GCAACCACGA AGGCGGCGGC GGCGAGGTGT CGAGGCAGGG CGTGCAGCGC GATCTGCTGC CACTCGTCGA AGGCACGACG ATCAACACGA AGTACGGGAT GGTGAAGACC GATCACATCC TGTTCATCGC GAGCGGCGCG TTCCATCTCG CGAAGCCGAG CGACCTGATT CCCGAGCTGC AAGGGCGCTT TCCGATCCGC GTCGAGCTCG ATTCGCTGTC GGTGAAGGAC TTCGAAGCGA TTCTCGTCGC GACCGACGCG AGCCTCGTCA AGCAGTATCA GGCGCTCCTC GCGACGGAGG ACGTCAAGCT CGAATTCGCC GACGACGGCA TTCGCCGGCT CGCCGAGATC GCATACGCGG TCAACGAGAA GACCGAGAAC ATCGGCGCGC GGCGCCTGTA CACGGTGATC GAGAAGCTGC TCGAGGAAGT GTCGTTCGCC GCGGGCAATC ATGCCGGCCA GAGCGTGACG ATCGATTCGG CCTATGTCGA TCACGCGCTC GGCGAAGTGT CGAAGGACGA GGATCTGTCG CGCTACGTGC TGTGAAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA