BupsA.00052.a
glyceraldehyde-3-phosphate dehydrogenase, type I
| CENTER ID: | BupsA.00052.a |
| ORGANISM: | Burkholderia pseudomallei 1710b |
| ASSOCIATED DISEASE: | Melioidosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BupsA.00052.a.A1.GE25023 | Full length( BupsA.00052.a ) | 1 | 336 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BupsA.00052.a.B1.PS00140 | Full length( BupsA.00052.a ) | 1 | 336 | ||
| BupsA.00052.a.A1.PW25061 | Full length( BupsA.00052.a ) | 1 | 336 |
Structures
3GNQ
DEPOSITED: 3/17/2009
DETERMINATION: XRay
CLONE: BupsA.00052.a.B1.GE25119
PROTEIN: BupsA.00052.a.B1.PS00140
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_126628 |
| PATRIC ID: | fig|320372.6.peg.3610 |
| RefSeq: | YP_334837 |
| UniProt: | Q3JNL6 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTIRVAINGY GRIGRNTLRA FYENGKKHDL EIVAINDLGD AKTNAHLTQY DTAHGKFPGE VSVDGDYLVV NGDRIRVLAN RNPAELPWGE LGVDVVMECT GFFTSKEKAS AHLKGGAKKV IISAPGGKDV DATIVYGVNH DVLKAEHTVI SNASCTTNCL APLVKPLNDK IGLETGLMTT IHAYTNDQVL TDVYHEDLRR ARSATHSQIP TKTGAAAAVG LVLPELNGKL DGYAIRVPTI NVSIVDLSFI AKRDTTAAEV NAIMKEASEG ALKGILGYNE APLVSIDFNH NPASSTFDAT LTKVSGRLVK VSSWYDNEWG FSNRMLDTAI ALANAK
NT Sequence
ATGACGATTC GCGTTGCAAT CAACGGTTAC GGCCGCATCG GCCGCAACAC GCTGCGCGCT TTCTACGAAA ACGGCAAGAA GCACGATCTC GAGATCGTTG CGATCAACGA TCTGGGCGAC GCGAAGACCA ACGCGCACCT GACGCAGTAC GACACCGCGC ACGGCAAGTT CCCGGGCGAA GTGTCGGTCG ACGGTGATTA CCTCGTCGTG AACGGCGACA GGATTCGCGT GCTCGCGAAC CGCAACCCGG CCGAGCTGCC GTGGGGCGAG CTGGGCGTCG ACGTCGTGAT GGAATGCACG GGCTTCTTCA CGTCGAAGGA AAAGGCGAGC GCGCACCTGA AGGGCGGCGC GAAGAAGGTG ATCATCTCGG CGCCGGGCGG CAAGGACGTC GACGCGACGA TCGTCTACGG CGTGAACCAC GACGTGCTGA AGGCCGAGCA CACCGTCATC TCGAACGCAT CGTGCACGAC GAACTGCCTG GCTCCGCTCG TCAAGCCGCT GAACGACAAG ATCGGCCTCG AAACCGGCCT GATGACGACG ATTCATGCCT ACACGAACGA CCAGGTGCTG ACGGACGTCT ACCACGAGGA CCTGCGCCGC GCGCGCTCGG CGACGCACAG CCAGATCCCG ACGAAGACGG GTGCGGCGGC CGCCGTCGGC CTCGTGCTGC CGGAGCTGAA CGGCAAGCTC GACGGCTACG CGATCCGCGT GCCGACCATC AACGTGTCGA TCGTCGATCT GTCGTTCATC GCGAAGCGCG ACACGACCGC GGCCGAAGTC AACGCGATCA TGAAGGAAGC GTCGGAAGGC GCGCTGAAGG GCATCCTCGG CTACAACGAG GCGCCGCTCG TGTCGATCGA CTTCAACCAC AACCCGGCTT CGTCGACGTT CGACGCGACG CTCACGAAGG TGTCGGGCCG TCTCGTGAAG GTGTCGAGCT GGTACGACAA CGAGTGGGGC TTCTCGAACC GCATGCTGGA TACGGCGATC GCGCTCGCGA ACGCGAAG
Details for BupsA.00052.a.A1.GE25023
| HARVESTED ON: | 3/31/2008 |
| SEQUENCED ON: | 7/29/2008 |
| EXPECTED MW: | 38kDa |
| OBSERVED MW: | 39kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Validated AA Sequence
MGTLEAQTQG PGSMTIRVAI NGYGRIGRNT LRAFYENGKK HDLEIVAIND LGDAKTNAHL TQYDTAHGKF PGEVSVDGDY LVVNGDRIRV LANRNPAELP WGELGVDVVM ECTGFFTSKE KASAHLKGGA KKVIISAPGG KDVDATIVYG VNHDVLKAEH TVISNASCTT NCLAPLVKPL NDKIGLETGL MTTIHAYTND QVLTDVYHED LRRARSATHS QIPTKTGAAA AVGLVLPELN GKLDGYAIRV PTINVSIVDL SFIAKRDTTA AEVNAIMKEA SEGALKGILG YNEAPLVSID FNHNPASSTF DATLTKVSGR LVKVSSWYDN EWGFSNRMLD TAIALANAK
Validated NT Sequence
ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatgacgatt cgcgttgcaa tcaacggtta cggccgcatc ggccgcaaca cgctgcgcgc tttctacgaa aacggcaaga agcacgatct cgagatcgtt gcgatcaacg atctgggcga cgcgaagacc aacgcgcacc tgacgcagta cgacaccgcg cacggcaagt tcccgggcga agtgtcggtc gacggtgatt acctcgtcgt gaacggcgac aggattcgcg tgctcgcgaa ccgcaacccg gccgagctgc cgtggggcga gctgggcgtc gacgtcgtga tggaatgcac gggcttcttc acgtcgaagg aaaaggcgag cgcgcacctg aagggcggcg cgaagaaggt gatcatctcg gcgccgggcg gcaaggacgt cgacgcgacg atcgtctacg gcgtgaacca cgacgtgctg aaggccgagc acaccgtcat ctcgaacgca tcgtgcacga cgaactgcct ggctccgctc gtcaagccgc tgaacgacaa gatcggcctc gaaaccggcc tgatgacgac gattcatgcc tacacgaacg accaggtgct gacggacgtc taccacgagg acctgcgccg cgcgcgctcg gcgacgcaca gccagatccc gacgaagacg ggtgcggcgg ccgccgtcgg cctcgtgctg ccggagctga acggcaagct cgacggctac gcgatccgcg tgccgaccat caacgtgtcg atcgtcgatc tgtcgttcat cgcgaagcgc gacacgaccg cggccgaagt caacgcgatc atgaaggaag cgtcggaagg cgcgctgaag ggcatcctcg gctacaacga ggcgccgctc gtgtcgatcg acttcaacca caacccggct tcgtcgacgt tcgacgcgac gctcacgaag gtgtcgggcc gtctcgtgaa ggtgtcgagc tggtacgaca acgagtgggg cttctcgaac cgcatgctgg atacggcgat cgcgctcgcg aacgcgaagt aataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTIRVAING YGRIGRNTLR AFYENGKKHD LEIVAINDLG DAKTNAHLTQ YDTAHGKFPG EVSVDGDYLV VNGDRIRVLA NRNPAELPWG ELGVDVVMEC TGFFTSKEKA SAHLKGGAKK VIISAPGGKD VDATIVYGVN HDVLKAEHTV ISNASCTTNC LAPLVKPLND KIGLETGLMT TIHAYTNDQV LTDVYHEDLR RARSATHSQI PTKTGAAAAV GLVLPELNGK LDGYAIRVPT INVSIVDLSF IAKRDTTAAE VNAIMKEASE GALKGILGYN EAPLVSIDFN HNPASSTFDA TLTKVSGRLV KVSSWYDNEW GFSNRMLDTA IALANAK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacgattcgc gttgcaatca acggttacgg ccgcatcggc cgcaacacgc tgcgcgcttt ctacgaaaac ggcaagaagc acgatctcga gatcgttgcg atcaacgatc tgggcgacgc gaagaccaac gcgcacctga cgcagtacga caccgcgcac ggcaagttcc cgggcgaagt gtcggtcgac ggtgattacc tcgtcgtgaa cggcgacagg attcgcgtgc tcgcgaaccg caacccggcc gagctgccgt ggggcgagct gggcgtcgac gtcgtgatgg aatgcacggg cttcttcacg tcgaaggaaa aggcgagcgc gcacctgaag ggcggcgcga agaaggtgat catctcggcg ccgggcggca aggacgtcga cgcgacgatc gtctacggcg tgaaccacga cgtgctgaag gccgagcaca ccgtcatctc gaacgcatcg tgcacgacga actgcctggc tccgctcgtc aagccgctga acgacaagat cggcctcgaa accggcctga tgacgacgat tcatgcctac acgaacgacc aggtgctgac ggacgtctac cacgaggacc tgcgccgcgc gcgctcggcg acgcacagcc agatcccgac gaagacgggt gcggcggccg ccgtcggcct cgtgctgccg gagctgaacg gcaagctcga cggctacgcg atccgcgtgc cgaccatcaa cgtgtcgatc gtcgatctgt cgttcatcgc gaagcgcgac acgaccgcgg ccgaagtcaa cgcgatcatg aaggaagcgt cggaaggcgc gctgaagggc atcctcggct acaacgaggc gccgctcgtg tcgatcgact tcaaccacaa cccggcttcg tcgacgttcg acgcgacgct cacgaaggtg tcgggccgtc tcgtgaaggt gtcgagctgg tacgacaacg agtggggctt ctcgaaccgc atgctggata cggcgatcgc gctcgcgaac gcgaagtgaa aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a
Details for BupsA.00052.a.B1.PS00140
| PURIFICATION DATe: | 11/6/2008 |
| CONCENTRATION: | 33.4mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 6 |
| VIAL VOLUME: | 150µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHXHHHHMT IRVAINGYGR IGRNTLRAFY ENGKKHDLEI VAINDLGDAK TNAHLTQYDT AHGKFPGEVS VDGDYLVVNG DRIRVLANRN PAELPWGELG VDVVMECTGF FTSKEKASAH LKGGAKKVII SAPGGKDVDA TIVYGVNHDV LKAEHTVISN ASCTTNCLAP LVKPLNDKIG LETGLMTTIH AYTNDQVLTD VYHEDLRRAR SATHSQIPTK TGAAAAVGLV LPELNGKLDG YAIRVPTINV SIVDLSFIAK RDTTAAEVNA IMKEASEGAL KGILGYNEAP LVSIDFNHNP ASSTFDATLT KVSGRLVKVS SWYDNEWGFS NRMLDTAIAL ANAK
Validated NT Sequence
tacttcgcgt tcgcgagcgc gatcgccgta tccagcatgc ggttcgagaa gccccactcg ttgtcgtacc agctcgacac cttcacgaga cggcccgaca ccttcgtgag cgtcgcgtcg aacgtcgacg aagccgggtt gtggttgaag tcgatcgaca cgagcggcgc ctcgttgtag ccgaggatgc ccttcagcgc gccttccgac gcttccttca tgatcgcgtt gacttcggcc gcggtcgtgt cgcgcttcgc gatgaacgac agatcgacga tcgacacgtt gatggtcggc acgcggatcg cgtagccgtc gagcttgccg ttcagctccg gcagcacgag gccgacggcg gccgccgcac ccgtcttcgt cgggatctgg ctgtgcgtcg ccgagcgcgc gcggcgcagg tcctcgtggt agacgtccgt cagcacctgg tcgttcgtgt aggcatgaat cgtcgtcatc aggccggttt cgaggccgat cttgtcgttc agcggcttga cgagcggagc caggcagttc gtcgtgcacg atgcgttcga gatgacggtg tgctcggcct tcagcacgtc gtggttcacg ccgtagacga tcgtcgcgtc gacgtccttg ccgcccggcg ccgagatgat caccttcttc gcgccgccct tcaggtgcgc gctcgccttt tccttcgacg tgaagaagcc cgtgcattcc atcacgacgt cgacgcccag ctcgccccac ggcagctcgg ccgggttgcg gttcgcgagc acgcgaatcc tgtcgccgtt cacgacgagg taatcaccgt cgaccgacac ttcgcccggg aacttgccgt gcgcggtgtc gtactgcgtc aggtgcgcgt tggtcttcgc gtcgcccaga tcgttgatcg caacgatctc gagatcgtgc ttcttgccgt tttcgtagaa agcgcgcagc gtgttgcggc cgatgcggcc gtaaccgttg attgcaacgc gaatcgtcat atggtggtgg tggnggtgag ccatggtntn nctccttctt a
Expressed Protein Sequence
MAHHHHHHMT IRVAINGYGR IGRNTLRAFY ENGKKHDLEI VAINDLGDAK TNAHLTQYDT AHGKFPGEVS VDGDYLVVNG DRIRVLANRN PAELPWGELG VDVVMECTGF FTSKEKASAH LKGGAKKVII SAPGGKDVDA TIVYGVNHDV LKAEHTVISN ASCTTNCLAP LVKPLNDKIG LETGLMTTIH AYTNDQVLTD VYHEDLRRAR SATHSQIPTK TGAAAAVGLV LPELNGKLDG YAIRVPTINV SIVDLSFIAK RDTTAAEVNA IMKEASEGAL KGILGYNEAP LVSIDFNHNP ASSTFDATLT KVSGRLVKVS SWYDNEWGFS NRMLDTAIAL ANAK
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACGA TTCGCGTTGC AATCAACGGT TACGGCCGCA TCGGCCGCAA CACGCTGCGC GCTTTCTACG AAAACGGCAA GAAGCACGAT CTCGAGATCG TTGCGATCAA CGATCTGGGC GACGCGAAGA CCAACGCGCA CCTGACGCAG TACGACACCG CGCACGGCAA GTTCCCGGGC GAAGTGTCGG TCGACGGTGA TTACCTCGTC GTGAACGGCG ACAGGATTCG CGTGCTCGCG AACCGCAACC CGGCCGAGCT GCCGTGGGGC GAGCTGGGCG TCGACGTCGT GATGGAATGC ACGGGCTTCT TCACGTCGAA GGAAAAGGCG AGCGCGCACC TGAAGGGCGG CGCGAAGAAG GTGATCATCT CGGCGCCGGG CGGCAAGGAC GTCGACGCGA CGATCGTCTA CGGCGTGAAC CACGACGTGC TGAAGGCCGA GCACACCGTC ATCTCGAACG CATCGTGCAC GACGAACTGC CTGGCTCCGC TCGTCAAGCC GCTGAACGAC AAGATCGGCC TCGAAACCGG CCTGATGACG ACGATTCATG CCTACACGAA CGACCAGGTG CTGACGGACG TCTACCACGA GGACCTGCGC CGCGCGCGCT CGGCGACGCA CAGCCAGATC CCGACGAAGA CGGGTGCGGC GGCCGCCGTC GGCCTCGTGC TGCCGGAGCT GAACGGCAAG CTCGACGGCT ACGCGATCCG CGTGCCGACC ATCAACGTGT CGATCGTCGA TCTGTCGTTC ATCGCGAAGC GCGACACGAC CGCGGCCGAA GTCAACGCGA TCATGAAGGA AGCGTCGGAA GGCGCGCTGA AGGGCATCCT CGGCTACAAC GAGGCGCCGC TCGTGTCGAT CGACTTCAAC CACAACCCGG CTTCGTCGAC GTTCGACGCG ACGCTCACGA AGGTGTCGGG CCGTCTCGTG AAGGTGTCGA GCTGGTACGA CAACGAGTGG GGCTTCTCGA ACCGCATGCT GGATACGGCG ATCGCGCTCG CGAACGCGAA GTGATGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT
Details for BupsA.00052.a.A1.PW25061
| PURIFICATION DATe: | 4/25/2008 |
| CONCENTRATION: | 21.8mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 3 |
| VIAL VOLUME: | data unavailable |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMTIRVAI NGYGRIGRNT LRAFYENGKK HDLEIVAIND LGDAKTNAHL TQYDTAHGKF PGEVSVDGDY LVVNGDRIRV LANRNPAELP WGELGVDVVM ECTGFFTSKE KASAHLKGGA KKVIISAPGG KDVDATIVYG VNHDVLKAEH TVISNASCTT NCLAPLVKPL NDKIGLETGL MTTIHAYTND QVLTDVYHED LRRARSATHS QIPTKTGAAA AVGLVLPELN GKLDGYAIRV PTINVSIVDL SFIAKRDTTA AEVNAIMKEA SEGALKGILG YNEAPLVSID FNHNPASSTF DATLTKVSGR LVKVSSWYDN EWGFSNRMLD TAIALANAK
Validated NT Sequence
ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatgacgatt cgcgttgcaa tcaacggtta cggccgcatc ggccgcaaca cgctgcgcgc tttctacgaa aacggcaaga agcacgatct cgagatcgtt gcgatcaacg atctgggcga cgcgaagacc aacgcgcacc tgacgcagta cgacaccgcg cacggcaagt tcccgggcga agtgtcggtc gacggtgatt acctcgtcgt gaacggcgac aggattcgcg tgctcgcgaa ccgcaacccg gccgagctgc cgtggggcga gctgggcgtc gacgtcgtga tggaatgcac gggcttcttc acgtcgaagg aaaaggcgag cgcgcacctg aagggcggcg cgaagaaggt gatcatctcg gcgccgggcg gcaaggacgt cgacgcgacg atcgtctacg gcgtgaacca cgacgtgctg aaggccgagc acaccgtcat ctcgaacgca tcgtgcacga cgaactgcct ggctccgctc gtcaagccgc tgaacgacaa gatcggcctc gaaaccggcc tgatgacgac gattcatgcc tacacgaacg accaggtgct gacggacgtc taccacgagg acctgcgccg cgcgcgctcg gcgacgcaca gccagatccc gacgaagacg ggtgcggcgg ccgccgtcgg cctcgtgctg ccggagctga acggcaagct cgacggctac gcgatccgcg tgccgaccat caacgtgtcg atcgtcgatc tgtcgttcat cgcgaagcgc gacacgaccg cggccgaagt caacgcgatc atgaaggaag cgtcggaagg cgcgctgaag ggcatcctcg gctacaacga ggcgccgctc gtgtcgatcg acttcaacca caacccggct tcgtcgacgt tcgacgcgac gctcacgaag gtgtcgggcc gtctcgtgaa ggtgtcgagc tggtacgaca acgagtgggg cttctcgaac cgcatgctgg atacggcgat cgcgctcgcg aacgcgaagt aataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTIRVAING YGRIGRNTLR AFYENGKKHD LEIVAINDLG DAKTNAHLTQ YDTAHGKFPG EVSVDGDYLV VNGDRIRVLA NRNPAELPWG ELGVDVVMEC TGFFTSKEKA SAHLKGGAKK VIISAPGGKD VDATIVYGVN HDVLKAEHTV ISNASCTTNC LAPLVKPLND KIGLETGLMT TIHAYTNDQV LTDVYHEDLR RARSATHSQI PTKTGAAAAV GLVLPELNGK LDGYAIRVPT INVSIVDLSF IAKRDTTAAE VNAIMKEASE GALKGILGYN EAPLVSIDFN HNPASSTFDA TLTKVSGRLV KVSSWYDNEW GFSNRMLDTA IALANAK
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACGATTCGC GTTGCAATCA ACGGTTACGG CCGCATCGGC CGCAACACGC TGCGCGCTTT CTACGAAAAC GGCAAGAAGC ACGATCTCGA GATCGTTGCG ATCAACGATC TGGGCGACGC GAAGACCAAC GCGCACCTGA CGCAGTACGA CACCGCGCAC GGCAAGTTCC CGGGCGAAGT GTCGGTCGAC GGTGATTACC TCGTCGTGAA CGGCGACAGG ATTCGCGTGC TCGCGAACCG CAACCCGGCC GAGCTGCCGT GGGGCGAGCT GGGCGTCGAC GTCGTGATGG AATGCACGGG CTTCTTCACG TCGAAGGAAA AGGCGAGCGC GCACCTGAAG GGCGGCGCGA AGAAGGTGAT CATCTCGGCG CCGGGCGGCA AGGACGTCGA CGCGACGATC GTCTACGGCG TGAACCACGA CGTGCTGAAG GCCGAGCACA CCGTCATCTC GAACGCATCG TGCACGACGA ACTGCCTGGC TCCGCTCGTC AAGCCGCTGA ACGACAAGAT CGGCCTCGAA ACCGGCCTGA TGACGACGAT TCATGCCTAC ACGAACGACC AGGTGCTGAC GGACGTCTAC CACGAGGACC TGCGCCGCGC GCGCTCGGCG ACGCACAGCC AGATCCCGAC GAAGACGGGT GCGGCGGCCG CCGTCGGCCT CGTGCTGCCG GAGCTGAACG GCAAGCTCGA CGGCTACGCG ATCCGCGTGC CGACCATCAA CGTGTCGATC GTCGATCTGT CGTTCATCGC GAAGCGCGAC ACGACCGCGG CCGAAGTCAA CGCGATCATG AAGGAAGCGT CGGAAGGCGC GCTGAAGGGC ATCCTCGGCT ACAACGAGGC GCCGCTCGTG TCGATCGACT TCAACCACAA CCCGGCTTCG TCGACGTTCG ACGCGACGCT CACGAAGGTG TCGGGCCGTC TCGTGAAGGT GTCGAGCTGG TACGACAACG AGTGGGGCTT CTCGAACCGC ATGCTGGATA CGGCGATCGC GCTCGCGAAC GCGAAGTGAA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A