BupsA.00040.a
S-adenosylmethionine synthetase
CENTER ID:
BupsA.00040.a
ORGANISM:
Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE:
Melioidosis
CURRENT STATUS:
in PDB
COMMUNITY REQUEST:
True
NIH RISK GROUP:
3
SELECT AGENT:
True
NIH PRIORITY
pathogens category:
IIIB
Ordering Clones & Proteins
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Clones*
CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO
AA
START
AA
STOP
ORDER
MATERIAL
BupsA.00040.a.A1.GE25014
Full length( BupsA.00040.a )
info
1
395
BupsA.00040.a.A1.GE25014
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis . Get sequence information
using the button in the "info" column.
Proteins
CENTER REFERENCE ID
DOMAIN/REGION DESCRIPTION
INFO
AA START
AA STOP
ORDER MATERIAL
BupsA.00040.a.A1.PW25052
Full length( BupsA.00040.a )
info
1
395
PW25052
Structures
3IML
DEPOSITED: 8/10/2009
DETERMINATION: XRay
CLONE: BupsA.00040.a.A1.GE25014
PROTEIN: BupsA.00040.a.A1.PW25052
Publications by SSGCID
Combining functional and structural genomics to sample the essential Burkholderia structome.
Abendroth J, Armour B, Barrett L, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Fox D, Gallagher LA, Gardberg AS, Gillespie A, Manoil C, Myler PJ, Nakazawa-Hewitt S, Napuli A, Nguyen MT, Patrapuvich R, Phan I, Stacy R, Staker BL, Stewart LJ, Van Voorhis WC
PLoS ONE - 2012
volume 8, issue 1, pages e53851
PDB:
3D63 ,
3DAH ,
3EIZ ,
3EJ2 ,
3EK2 ,
3EZO ,
3F0F ,
3FTP ,
3GK0 ,
3GK3 ,
3GVF ,
3GWA ,
3GWE ,
3IML ,
3SZ8 ,
3T4C ,
3TML ,
3TMQ ,
3TXY ,
3U7J ,
3UE9 ,
3UK1 ,
3UK2 ,
3UND ,
3UPT ,
3URR ,
3UW1 ,
3UW2 ,
3UW3 ,
3V2I ,
3V7N ,
3V8H ,
3V9O ,
3V9P ,
3VAV ,
4DDO ,
4DFE ,
4DHE ,
4DHK ,
4DUT ,
4DZ4 ,
4E4T ,
4E51 ,
4EFI ,
4EG0 ,
4EGJ ,
4EK2 ,
4EQY ,
4EWG ,
4EX5 ,
4EXQ ,
4F2G ,
4F32 ,
4F3N ,
4F3Y ,
4F4H ,
4F7D ,
4FK8 ,
4FRY ,
4G1K ,
4G67 ,
4G6Z ,
4GHK ,
4H3Y ,
4H3Z ,
4H4G
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MANDYLFTSE SVSEGHPDKV ADQISDAILD AILAQDKYSR VAAETLCNTG LVVLAGEITT TANIDYIQIA RDTIKRIGYD NTDYGIDYRG CAVLVAYDKQ SPDIAQGVDR AHDNNLDQGA GDQGLMFGYA CDETPELMPL PIHLSHRLVE RQANLRRDGR LPWLRPDAKS QVTVRYVDGK PHSIDTVVLS TQHAPEIDLP ALREAVIEEV IKPTLPADLI KGDIKFLVNP TGRFVIGGPQ GDCGLTGRKI IVDTYGGAAP HGGGAFSGKD PSKVDRSAAY AGRYVAKNIV AAGLASRALI QVSYAIGVAE PTSVMVNTFG TGRVSDETIT KLVREHFDLR PKGIIQMLDL LRPIYEKTAA YGHFGREEPE FSWEAADKAL ALAEAAGVEP AVQVA
NT Sequence
GTGGCAAACG ATTATCTCTT CACGTCCGAA TCCGTTTCCG AAGGCCATCC GGACAAGGTC GCCGACCAAA TCTCCGACGC GATCCTCGAC GCAATCCTCG CGCAAGACAA GTACTCCCGT GTCGCGGCCG AAACGCTGTG CAACACCGGT CTCGTCGTGC TCGCCGGCGA AATCACGACC ACGGCGAACA TCGACTACAT CCAGATCGCG CGCGACACGA TCAAGCGCAT CGGCTACGAC AATACCGATT ACGGCATCGA CTACCGCGGC TGCGCGGTGC TCGTCGCGTA CGACAAGCAG TCGCCGGACA TCGCGCAAGG CGTCGACCGC GCGCACGACA ACAACCTCGA TCAGGGCGCG GGCGATCAGG GCCTGATGTT CGGCTACGCG TGCGACGAAA CGCCCGAGCT GATGCCGCTG CCGATCCACC TGTCGCACCG GCTCGTCGAG CGCCAGGCGA ACCTGCGCCG CGACGGCCGC CTGCCGTGGC TGCGCCCGGA CGCGAAGTCA CAGGTGACGG TGCGCTACGT CGACGGCAAG CCGCACTCGA TCGACACCGT CGTGCTGTCG ACGCAGCACG CGCCGGAGAT CGATCTGCCC GCGCTGCGCG AAGCGGTGAT CGAAGAGGTC ATCAAGCCGA CGCTGCCCGC CGACCTGATC AAGGGCGACA TCAAGTTCCT CGTGAACCCG ACCGGCCGCT TCGTGATCGG CGGCCCGCAA GGCGATTGCG GGCTGACCGG CCGCAAGATC ATCGTCGACA CCTACGGCGG CGCGGCGCCG CACGGCGGCG GCGCGTTCTC GGGCAAGGAT CCGTCGAAGG TCGACCGCTC GGCCGCCTAC GCGGGCCGCT ACGTCGCGAA GAACATCGTC GCCGCGGGCC TCGCGTCGCG CGCGCTGATT CAGGTGTCGT ACGCGATCGG CGTGGCCGAG CCGACCTCGG TGATGGTCAA CACGTTCGGC ACGGGCCGCG TGTCCGACGA GACGATCACG AAGCTCGTGC GCGAGCACTT CGACCTGCGT CCGAAGGGCA TCATCCAGAT GCTCGACCTG CTGCGCCCGA TCTACGAGAA GACCGCCGCT TACGGCCACT TCGGCCGCGA AGAACCGGAG TTCTCGTGGG AAGCCGCCGA CAAGGCGCTC GCGCTCGCCG AAGCGGCGGG CGTCGAGCCG GCCGTGCAAG TGGCC
Details for BupsA.00040.a.A1.GE25014
HARVESTED ON:
4/7/2008
SEQUENCED ON:
6/16/2009
EXPECTED MW:
45kDa
OBSERVED MW:
46kDa
ANTIBIOTIC MARKER:
ampicillin
COUNT OF EXPRESSION COLONIES:
Too many to count (100+)
TOTAL EXPRESSION LEVEL:
High Expression
SOLUBLE EXPRESSION LEVEL
High Expression
EXPRESSION HOST:
data unavailable
SEQUENCING RESULT:
pass with incomplete coverage
PERCENT IDENTITY:
99
PERCENT COVERAGE:
73
Details for BupsA.00040.a.A1.PW25052
PURIFICATION DATe:
6/8/2009
CONCENTRATION:
25.79mg/ml
OBSERVED MW:
data unavailable
EXPRESSION LEVEL:
Low Expression
PROTEIN PURIFICATION BUFFER:
25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:
data unavailable
VIAL COUNT (approx.):
3
VIAL VOLUME:
100µl
PERCENT IDENTITY:
99
PERCENT COVERAGE:
73