BupsA.00040.a
S-adenosylmethionine synthetase
| CENTER ID: | BupsA.00040.a |
| ORGANISM: | Burkholderia pseudomallei 1710b |
| ASSOCIATED DISEASE: | Melioidosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BupsA.00040.a.A1.GE25014 | Full length( BupsA.00040.a ) | 1 | 395 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BupsA.00040.a.A1.PW25052 | Full length( BupsA.00040.a ) | 1 | 395 |
Structures
3IML
DEPOSITED: 8/10/2009
DETERMINATION: XRay
CLONE: BupsA.00040.a.A1.GE25014
PROTEIN: BupsA.00040.a.A1.PW25052
Publications by SSGCID
Combining functional and structural genomics to sample the essential Burkholderia structome.
Abendroth J, Armour B, Barrett L, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Fox D, Gallagher LA, Gardberg AS, Gillespie A, Manoil C, Myler PJ, Nakazawa-Hewitt S, Napuli A, Nguyen MT, Patrapuvich R, Phan I, Stacy R, Staker BL, Stewart LJ, Van Voorhis WC
PLoS ONE - 2012
volume 8, issue 1, pages e53851
PMID: 23382856; PMCID: PMC3561365
PDB:
3D63,
3DAH,
3EIZ,
3EJ2,
3EK2,
3EZO,
3F0F,
3FTP,
3GK0,
3GK3,
3GVF,
3GWA,
3GWE,
3IML,
3SZ8,
3T4C,
3TML,
3TMQ,
3TXY,
3U7J,
3UE9,
3UK1,
3UK2,
3UND,
3UPT,
3URR,
3UW1,
3UW2,
3UW3,
3V2I,
3V7N,
3V8H,
3V9O,
3V9P,
3VAV,
4DDO,
4DFE,
4DHE,
4DHK,
4DUT,
4DZ4,
4E4T,
4E51,
4EFI,
4EG0,
4EGJ,
4EK2,
4EQY,
4EWG,
4EX5,
4EXQ,
4F2G,
4F32,
4F3N,
4F3Y,
4F4H,
4F7D,
4FK8,
4FRY,
4G1K,
4G67,
4G6Z,
4GHK,
4H3Y,
4H3Z,
4H4G
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_126734 |
| PATRIC ID: | fig|320372.6.peg.397 |
| RefSeq: | YP_331809 |
| UniProt: | Q3JX94 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MANDYLFTSE SVSEGHPDKV ADQISDAILD AILAQDKYSR VAAETLCNTG LVVLAGEITT TANIDYIQIA RDTIKRIGYD NTDYGIDYRG CAVLVAYDKQ SPDIAQGVDR AHDNNLDQGA GDQGLMFGYA CDETPELMPL PIHLSHRLVE RQANLRRDGR LPWLRPDAKS QVTVRYVDGK PHSIDTVVLS TQHAPEIDLP ALREAVIEEV IKPTLPADLI KGDIKFLVNP TGRFVIGGPQ GDCGLTGRKI IVDTYGGAAP HGGGAFSGKD PSKVDRSAAY AGRYVAKNIV AAGLASRALI QVSYAIGVAE PTSVMVNTFG TGRVSDETIT KLVREHFDLR PKGIIQMLDL LRPIYEKTAA YGHFGREEPE FSWEAADKAL ALAEAAGVEP AVQVA
NT Sequence
GTGGCAAACG ATTATCTCTT CACGTCCGAA TCCGTTTCCG AAGGCCATCC GGACAAGGTC GCCGACCAAA TCTCCGACGC GATCCTCGAC GCAATCCTCG CGCAAGACAA GTACTCCCGT GTCGCGGCCG AAACGCTGTG CAACACCGGT CTCGTCGTGC TCGCCGGCGA AATCACGACC ACGGCGAACA TCGACTACAT CCAGATCGCG CGCGACACGA TCAAGCGCAT CGGCTACGAC AATACCGATT ACGGCATCGA CTACCGCGGC TGCGCGGTGC TCGTCGCGTA CGACAAGCAG TCGCCGGACA TCGCGCAAGG CGTCGACCGC GCGCACGACA ACAACCTCGA TCAGGGCGCG GGCGATCAGG GCCTGATGTT CGGCTACGCG TGCGACGAAA CGCCCGAGCT GATGCCGCTG CCGATCCACC TGTCGCACCG GCTCGTCGAG CGCCAGGCGA ACCTGCGCCG CGACGGCCGC CTGCCGTGGC TGCGCCCGGA CGCGAAGTCA CAGGTGACGG TGCGCTACGT CGACGGCAAG CCGCACTCGA TCGACACCGT CGTGCTGTCG ACGCAGCACG CGCCGGAGAT CGATCTGCCC GCGCTGCGCG AAGCGGTGAT CGAAGAGGTC ATCAAGCCGA CGCTGCCCGC CGACCTGATC AAGGGCGACA TCAAGTTCCT CGTGAACCCG ACCGGCCGCT TCGTGATCGG CGGCCCGCAA GGCGATTGCG GGCTGACCGG CCGCAAGATC ATCGTCGACA CCTACGGCGG CGCGGCGCCG CACGGCGGCG GCGCGTTCTC GGGCAAGGAT CCGTCGAAGG TCGACCGCTC GGCCGCCTAC GCGGGCCGCT ACGTCGCGAA GAACATCGTC GCCGCGGGCC TCGCGTCGCG CGCGCTGATT CAGGTGTCGT ACGCGATCGG CGTGGCCGAG CCGACCTCGG TGATGGTCAA CACGTTCGGC ACGGGCCGCG TGTCCGACGA GACGATCACG AAGCTCGTGC GCGAGCACTT CGACCTGCGT CCGAAGGGCA TCATCCAGAT GCTCGACCTG CTGCGCCCGA TCTACGAGAA GACCGCCGCT TACGGCCACT TCGGCCGCGA AGAACCGGAG TTCTCGTGGG AAGCCGCCGA CAAGGCGCTC GCGCTCGCCG AAGCGGCGGG CGTCGAGCCG GCCGTGCAAG TGGCC
Details for BupsA.00040.a.A1.GE25014
| HARVESTED ON: | 4/7/2008 |
| SEQUENCED ON: | 6/16/2009 |
| EXPECTED MW: | 45kDa |
| OBSERVED MW: | 46kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 73 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVANDYLFT SESVSEGHPD KVADQISDAI LDAILAQDKY SRVAAETLCN TGLVVLAGEI TTTANIDYIQ IARDTIKRIG YDNTDYGIDY RGCAVLVAYD KQSPDIAQGV DRAHDNNLDQ GAGDQGLMFG YACDETPELM PLPIHLSHRL VERQANLRRD GRLPWLRPDA KSQVTVRYVD GKPHSIDTVV LSTQHAPEID LPALREAVIE EVIKPTLPAD LIKGDIKFLV NPTGRFVIGG PQGDCGLTGR KIIVDTYGGA APHGGXAFSG KDPSKXXRSA AYAGRY
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtgg caaacgatta tctcttcacg tccgaatccg tttccgaagg ccatccggac aaggtcgccg accaaatctc cgacgcgatc ctcgacgcaa tcctcgcgca agacaagtac tcccgtgtcg cggccgaaac gctgtgcaac accggtctcg tcgtgctcgc cggcgaaatc acgaccacgg cgaacatcga ctacatccag atcgcgcgcg acacgatcaa gcgcatcggc tacgacaata ccgattacgg catcgactac cgcggctgcg cggtgctcgt cgcgtacgac aagcagtcgc cggacatcgc gcaaggcgtc gaccgcgcgc acgacaacaa cctcgatcag ggcgcgggcg atcagggcct gatgttcggc tacgcgtgcg acgaaacgcc cgagctgatg ccgctgccga tccacctgtc gcaccggctc gtcgagcgcc aggcgaacct gcgccgcgac ggccgcctgc cgtggctgcg cccggacgcg aagtcacagg tgacggtgcg ctacgtcgac ggcaagccgc actcgatcga caccgtcgtg ctgtcgacgc agcacgcgcc ggagatcgat ctgcccgcgc tgcgcgaagc ggtgatcgaa gaggtcatca agccgacgct gcccgccgac ctgatcaagg gcgacatcaa gttcctcgtg aacccgaccg gccgcttcgt gatcggcggc ccgcaaggcg attgcgggct gaccggccgc aagatcatcg tcgacaccta cggcggcgcg gcgccgcacg gnggcngcgc gttctcgggc aaggatccgt cgaaggncga ncgctcggcc gcctacgcgg gncgctacgt c
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVANDYLFT SESVSEGHPD KVADQISDAI LDAILAQDKY SRVAAETLCN TGLVVLAGEI TTTANIDYIQ IARDTIKRIG YDNTDYGIDY RGCAVLVAYD KQSPDIAQGV DRAHDNNLDQ GAGDQGLMFG YACDETPELM PLPIHLSHRL VERQANLRRD GRLPWLRPDA KSQVTVRYVD GKPHSIDTVV LSTQHAPEID LPALREAVIE EVIKPTLPAD LIKGDIKFLV NPTGRFVIGG PQGDCGLTGR KIIVDTYGGA APHGGGAFSG KDPSKVDRSA AYAGRYVAKN IVAAGLASRA LIQVSYAIGV AEPTSVMVNT FGTGRVSDET ITKLVREHFD LRPKGIIQML DLLRPIYEKT AAYGHFGREE PEFSWEAADK ALALAEAAGV EPAVQVA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtggcaaac gattatctct tcacgtccga atccgtttcc gaaggccatc cggacaaggt cgccgaccaa atctccgacg cgatcctcga cgcaatcctc gcgcaagaca agtactcccg tgtcgcggcc gaaacgctgt gcaacaccgg tctcgtcgtg ctcgccggcg aaatcacgac cacggcgaac atcgactaca tccagatcgc gcgcgacacg atcaagcgca tcggctacga caataccgat tacggcatcg actaccgcgg ctgcgcggtg ctcgtcgcgt acgacaagca gtcgccggac atcgcgcaag gcgtcgaccg cgcgcacgac aacaacctcg atcagggcgc gggcgatcag ggcctgatgt tcggctacgc gtgcgacgaa acgcccgagc tgatgccgct gccgatccac ctgtcgcacc ggctcgtcga gcgccaggcg aacctgcgcc gcgacggccg cctgccgtgg ctgcgcccgg acgcgaagtc acaggtgacg gtgcgctacg tcgacggcaa gccgcactcg atcgacaccg tcgtgctgtc gacgcagcac gcgccggaga tcgatctgcc cgcgctgcgc gaagcggtga tcgaagaggt catcaagccg acgctgcccg ccgacctgat caagggcgac atcaagttcc tcgtgaaccc gaccggccgc ttcgtgatcg gcggcccgca aggcgattgc gggctgaccg gccgcaagat catcgtcgac acctacggcg gcgcggcgcc gcacggcggc ggcgcgttct cgggcaagga tccgtcgaag gtcgaccgct cggccgccta cgcgggccgc tacgtcgcga agaacatcgt cgccgcgggc ctcgcgtcgc gcgcgctgat tcaggtgtcg tacgcgatcg gcgtggccga gccgacctcg gtgatggtca acacgttcgg cacgggccgc gtgtccgacg agacgatcac gaagctcgtg cgcgagcact tcgacctgcg tccgaagggc atcatccaga tgctcgacct gctgcgcccg atctacgaga agaccgccgc ttacggccac ttcggccgcg aagaaccgga gttctcgtgg gaagccgccg acaaggcgct cgcgctcgcc gaagcggcgg gcgtcgagcc ggccgtgcaa gtggcctgaa aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a
Details for BupsA.00040.a.A1.PW25052
| PURIFICATION DATe: | 6/8/2009 |
| CONCENTRATION: | 25.79mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 3 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 73 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVANDYLFT SESVSEGHPD KVADQISDAI LDAILAQDKY SRVAAETLCN TGLVVLAGEI TTTANIDYIQ IARDTIKRIG YDNTDYGIDY RGCAVLVAYD KQSPDIAQGV DRAHDNNLDQ GAGDQGLMFG YACDETPELM PLPIHLSHRL VERQANLRRD GRLPWLRPDA KSQVTVRYVD GKPHSIDTVV LSTQHAPEID LPALREAVIE EVIKPTLPAD LIKGDIKFLV NPTGRFVIGG PQGDCGLTGR KIIVDTYGGA APHGGXAFSG KDPSKXXRSA AYAGRY
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtgg caaacgatta tctcttcacg tccgaatccg tttccgaagg ccatccggac aaggtcgccg accaaatctc cgacgcgatc ctcgacgcaa tcctcgcgca agacaagtac tcccgtgtcg cggccgaaac gctgtgcaac accggtctcg tcgtgctcgc cggcgaaatc acgaccacgg cgaacatcga ctacatccag atcgcgcgcg acacgatcaa gcgcatcggc tacgacaata ccgattacgg catcgactac cgcggctgcg cggtgctcgt cgcgtacgac aagcagtcgc cggacatcgc gcaaggcgtc gaccgcgcgc acgacaacaa cctcgatcag ggcgcgggcg atcagggcct gatgttcggc tacgcgtgcg acgaaacgcc cgagctgatg ccgctgccga tccacctgtc gcaccggctc gtcgagcgcc aggcgaacct gcgccgcgac ggccgcctgc cgtggctgcg cccggacgcg aagtcacagg tgacggtgcg ctacgtcgac ggcaagccgc actcgatcga caccgtcgtg ctgtcgacgc agcacgcgcc ggagatcgat ctgcccgcgc tgcgcgaagc ggtgatcgaa gaggtcatca agccgacgct gcccgccgac ctgatcaagg gcgacatcaa gttcctcgtg aacccgaccg gccgcttcgt gatcggcggc ccgcaaggcg attgcgggct gaccggccgc aagatcatcg tcgacaccta cggcggcgcg gcgccgcacg gnggcngcgc gttctcgggc aaggatccgt cgaaggncga ncgctcggcc gcctacgcgg gncgctacgt c
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVANDYLFT SESVSEGHPD KVADQISDAI LDAILAQDKY SRVAAETLCN TGLVVLAGEI TTTANIDYIQ IARDTIKRIG YDNTDYGIDY RGCAVLVAYD KQSPDIAQGV DRAHDNNLDQ GAGDQGLMFG YACDETPELM PLPIHLSHRL VERQANLRRD GRLPWLRPDA KSQVTVRYVD GKPHSIDTVV LSTQHAPEID LPALREAVIE EVIKPTLPAD LIKGDIKFLV NPTGRFVIGG PQGDCGLTGR KIIVDTYGGA APHGGGAFSG KDPSKVDRSA AYAGRYVAKN IVAAGLASRA LIQVSYAIGV AEPTSVMVNT FGTGRVSDET ITKLVREHFD LRPKGIIQML DLLRPIYEKT AAYGHFGREE PEFSWEAADK ALALAEAAGV EPAVQVA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGGCAAAC GATTATCTCT TCACGTCCGA ATCCGTTTCC GAAGGCCATC CGGACAAGGT CGCCGACCAA ATCTCCGACG CGATCCTCGA CGCAATCCTC GCGCAAGACA AGTACTCCCG TGTCGCGGCC GAAACGCTGT GCAACACCGG TCTCGTCGTG CTCGCCGGCG AAATCACGAC CACGGCGAAC ATCGACTACA TCCAGATCGC GCGCGACACG ATCAAGCGCA TCGGCTACGA CAATACCGAT TACGGCATCG ACTACCGCGG CTGCGCGGTG CTCGTCGCGT ACGACAAGCA GTCGCCGGAC ATCGCGCAAG GCGTCGACCG CGCGCACGAC AACAACCTCG ATCAGGGCGC GGGCGATCAG GGCCTGATGT TCGGCTACGC GTGCGACGAA ACGCCCGAGC TGATGCCGCT GCCGATCCAC CTGTCGCACC GGCTCGTCGA GCGCCAGGCG AACCTGCGCC GCGACGGCCG CCTGCCGTGG CTGCGCCCGG ACGCGAAGTC ACAGGTGACG GTGCGCTACG TCGACGGCAA GCCGCACTCG ATCGACACCG TCGTGCTGTC GACGCAGCAC GCGCCGGAGA TCGATCTGCC CGCGCTGCGC GAAGCGGTGA TCGAAGAGGT CATCAAGCCG ACGCTGCCCG CCGACCTGAT CAAGGGCGAC ATCAAGTTCC TCGTGAACCC GACCGGCCGC TTCGTGATCG GCGGCCCGCA AGGCGATTGC GGGCTGACCG GCCGCAAGAT CATCGTCGAC ACCTACGGCG GCGCGGCGCC GCACGGCGGC GGCGCGTTCT CGGGCAAGGA TCCGTCGAAG GTCGACCGCT CGGCCGCCTA CGCGGGCCGC TACGTCGCGA AGAACATCGT CGCCGCGGGC CTCGCGTCGC GCGCGCTGAT TCAGGTGTCG TACGCGATCG GCGTGGCCGA GCCGACCTCG GTGATGGTCA ACACGTTCGG CACGGGCCGC GTGTCCGACG AGACGATCAC GAAGCTCGTG CGCGAGCACT TCGACCTGCG TCCGAAGGGC ATCATCCAGA TGCTCGACCT GCTGCGCCCG ATCTACGAGA AGACCGCCGC TTACGGCCAC TTCGGCCGCG AAGAACCGGA GTTCTCGTGG GAAGCCGCCG ACAAGGCGCT CGCGCTCGCC GAAGCGGCGG GCGTCGAGCC GGCCGTGCAA GTGGCCTGAA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A