BupsA.00039.b

methionine aminopeptidase

CENTER ID: BupsA.00039.b
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: diffraction
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.00039.b.A1.GE25013 Full length( BupsA.00039.b ) 1 282
BupsA.00039.b.A2.GE27935 Created from Decode construct for feature BupsA.00039.b 1 264
BupsA.00039.b.A3.GE27936 Created from Decode construct for feature BupsA.00039.b 1 277
BupsA.00039.b.A5.GE27938 Created from Decode construct for feature BupsA.00039.b 15 264
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.00039.b.A3.PW27883 Created from Decode construct for feature BupsA.00039.b 1 277
BupsA.00039.b.A5.PW27885 Created from Decode construct for feature BupsA.00039.b 15 264

External Resources

RESOURCE REFERENCE ID
OrthoMCL: OG5_126721
PATRIC ID: fig|320372.6.peg.2710
RefSeq: YP_333976
UniProt: Q3JR27

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRNSPHFRHR LMAITLKNEH DIAQMRIACR LASEVLDYIT PFVVAGVTTG ELDRLCHEYM THVQGTVPAP LNYQPPGYPP FPKAICTSVN DVICHGIPGE KTLKNGDALN IDITVIKNGY FGDTSRMFII GEGSILAKRL VQTTYECMWL GIDQVRPGAH LGDIGHAIQK HAEAQGYSVV REYCGHGIGT VFHEDPQVVH YGRPGTGLEL KPGMIFTIEP MINAGKRDIR TMPDQWTVKT RDRSLSAQWE HTILVTDTGH EVLTVSAGTP ARPTFAAPAT AA
NT Sequence
TTGCGCAATT CACCGCATTT CAGGCATCGA CTCATGGCTA TTACGCTCAA AAACGAACAC GACATCGCGC AGATGCGCAT CGCCTGCCGT CTCGCGAGCG AAGTGCTCGA CTACATCACG CCATTCGTCG TCGCCGGCGT GACGACGGGC GAACTCGATC GCCTCTGCCA CGAATACATG ACCCATGTGC AGGGCACGGT GCCCGCGCCG CTCAACTACC AGCCGCCCGG CTATCCGCCG TTCCCGAAGG CGATCTGCAC GTCGGTCAAC GACGTGATCT GCCACGGCAT CCCGGGCGAG AAGACGCTCA AGAACGGCGA CGCGTTGAAC ATCGACATCA CCGTCATCAA GAACGGCTAT TTCGGCGACA CGAGCCGGAT GTTCATCATC GGCGAGGGCT CGATCCTCGC GAAGCGCCTC GTGCAAACCA CCTACGAATG CATGTGGCTC GGCATCGACC AGGTGAGGCC CGGCGCGCAT CTCGGCGACA TCGGCCACGC GATCCAGAAG CATGCGGAAG CGCAAGGCTA CAGCGTCGTG CGCGAATACT GCGGTCACGG CATCGGCACG GTGTTCCACG AGGACCCGCA AGTCGTCCAC TACGGGCGCC CGGGCACGGG CCTCGAGCTC AAGCCGGGCA TGATCTTCAC GATCGAGCCG ATGATCAACG CCGGCAAGCG CGACATCCGC ACGATGCCCG ACCAGTGGAC CGTCAAGACC CGCGACCGCA GCCTGTCCGC GCAGTGGGAG CACACGATCC TCGTCACCGA CACGGGTCAC GAGGTGTTGA CGGTGTCGGC CGGCACGCCG GCGCGCCCGA CGTTCGCCGC GCCGGCGACG GCGGCT
Details for BupsA.00039.b.A1.GE25013
HARVESTED ON: 2/12/2008
SEQUENCED ON: 7/29/2008
EXPECTED MW: 33kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMLRNSPHFR HRLMAITLKN EHDIAQMRIA CRLASEVLDY ITPFVVAGVT TGELDRLCHE YMTHVQGTVP APLNYQPPGY PPFPKAICTS VNDVICHGIP GEKTLKNGDA LNIDITVIKN GYFGDTSRMF IIGEGSILAK RLVQTTYECM WLGIDQVRPG AHLGDIGHAI QKHAEAQGYS VVREYCGHGI GTVFHEDPQV VHYGRPGTGL ELKPGMIFTI EPMINAGKRD IRTMPDQWTV KTRDRSLSAQ WEHTILVTDT GHEVLTVSAG TPARPTFAAP ATAA
Validated NT Sequence
tttaagaagg agnnatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgttgcg caattcaccg catttcaggc atcgactcat ggctattacg ctcaaaaacg aacacgacat cgcgcagatg cgcatcgcct gccgtctcgc gagcgaagtg ctcgactaca tcacgccatt cgtcgtcgcc ggcgtgacga cgggcgaact cgatcgcctc tgccacgaat acatgaccca tgtgcagggc acggtgcccg cgccgctcaa ctaccagccg cccggctatc cgccgttccc gaaggcgatc tgcacgtcgg tcaacgacgt gatctgccac ggcatcccgg gcgagaagac gctcaagaac ggcgacgcgt tgaacatcga catcaccgtc atcaagaacg gctatttcgg cgacacgagc cggatgttca tcatcggcga gggctcgatc ctcgcgaagc gcctcgtgca aaccacctac gaatgcatgt ggctcggcat cgaccaggtg aggcccggcg cgcatctcgg cgacatcggc cacgcgatcc agaagcatgc ggaagcgcaa ggctacagcg tcgtgcgcga atactgcggt cacggcatcg gcacggtgtt ccacgaggac ccgcaagtcg tccactacgg gcgcccgggc acgggcctcg agctcaagcc gggcatgatc ttcacgatcg agccgatgat caacgccggc aagcgcgaca tccgcacgat gcccgaccag tggaccgtca agacccgcga ccgcagcctg tccgcgcagt gggagcacac gatcctcgtc accgacacgg gtcacgaggt gttgacggtg tcggccggca cgccggcgcg cccgacgttc gccgcgccgg cgacggcggc ttaataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLRNSPHFR HRLMAITLKN EHDIAQMRIA CRLASEVLDY ITPFVVAGVT TGELDRLCHE YMTHVQGTVP APLNYQPPGY PPFPKAICTS VNDVICHGIP GEKTLKNGDA LNIDITVIKN GYFGDTSRMF IIGEGSILAK RLVQTTYECM WLGIDQVRPG AHLGDIGHAI QKHAEAQGYS VVREYCGHGI GTVFHEDPQV VHYGRPGTGL ELKPGMIFTI EPMINAGKRD IRTMPDQWTV KTRDRSLSAQ WEHTILVTDT GHEVLTVSAG TPARPTFAAP ATAA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttgcgcaat tcaccgcatt tcaggcatcg actcatggct attacgctca aaaacgaaca cgacatcgcg cagatgcgca tcgcctgccg tctcgcgagc gaagtgctcg actacatcac gccattcgtc gtcgccggcg tgacgacggg cgaactcgat cgcctctgcc acgaatacat gacccatgtg cagggcacgg tgcccgcgcc gctcaactac cagccgcccg gctatccgcc gttcccgaag gcgatctgca cgtcggtcaa cgacgtgatc tgccacggca tcccgggcga gaagacgctc aagaacggcg acgcgttgaa catcgacatc accgtcatca agaacggcta tttcggcgac acgagccgga tgttcatcat cggcgagggc tcgatcctcg cgaagcgcct cgtgcaaacc acctacgaat gcatgtggct cggcatcgac caggtgaggc ccggcgcgca tctcggcgac atcggccacg cgatccagaa gcatgcggaa gcgcaaggct acagcgtcgt gcgcgaatac tgcggtcacg gcatcggcac ggtgttccac gaggacccgc aagtcgtcca ctacgggcgc ccgggcacgg gcctcgagct caagccgggc atgatcttca cgatcgagcc gatgatcaac gccggcaagc gcgacatccg cacgatgccc gaccagtgga ccgtcaagac ccgcgaccgc agcctgtccg cgcagtggga gcacacgatc ctcgtcaccg acacgggtca cgaggtgttg acggtgtcgg ccggcacgcc ggcgcgcccg acgttcgccg cgccggcgac ggcggcttga aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for BupsA.00039.b.A2.GE27935
HARVESTED ON: 11/24/2009
SEQUENCED ON: 12/3/2009
EXPECTED MW: 31kDa
OBSERVED MW: 31kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMLRNSPHFR HRLMAITLKN EHDIAQMRIA CRLASEVLDY ITPFVVAGVT TGELDRLCHE YMTHVQGTVP APLNYQPPGY PPFPKAICTS VNDVICHGIP GEKTLKNGDA LNIDITVIKN GYFGDTSRMF IIGEGSILAK RLVQTTYECM WLGIDQVRPG AHLGDIGHAI QKHAEAQGYS VVREYCGHGI GTVFHEDPQV VHYGRPGTGL ELKPGMIFTI EPMINAGKRD IRTMPDQWTV KTRDRSLSAQ WEHTILVTDT GHEVLT
Validated NT Sequence
ttattacgtc aacacctcgt gacccgtgtc ggtgacgagg atcgtgtgct cccactgcgc ggacaggctg cggtcgcggg tcttgacggt ccactggtcg ggcatcgtgc ggatgtcgcg cttgccggcg ttgatcatcg gctcgatcgt gaagatcatg cccggcttga gctcgaggcc cgtgcccggg cgcccgtagt ggacgacttg cgggtcctcg tggaacaccg tgccgatgcc gtgaccgcag tattcgcgca cgacgctgta gccttgcgct tccgcatgct tctggatcgc gtggccgatg tcgccgagat gcgcgccggg cctcacctgg tcgatgccga gccacatgca ttcgtaggtg gtttgcacga ggcgcttcgc gaggatcgag ccctcgccga tgatgaacat ccggctcgtg tcgccgaaat agccgttctt gatgacggtg atgtcgatgt tcaacgcgtc gccgttcttg agcgtcttct cgcccgggat gccgtggcag atcacgtcgt tgaccgacgt gcagatcgcc ttcgggaacg gcggatagcc gggcggctgg tagttgagcg gcgcgggcac cgtgccctgc acatgggtca tgtattcgtg gcagaggcga tcgagttcgc ccgtcgtcac gccggcgacg acgaatggcg tgatgtagtc gagcacttcg ctcgcgagac ggcaggcgat gcgcatctgc gcgatgtcgt gttcgttttt gagcgtaata gccatgagtc gatgcctgaa atgcggtgaa ttgcgcaaca tcgaaccagg accctgggtc tgagcttcca gggtacccat atgatggtga tggtgatgag ccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLRNSPHFR HRLMAITLKN EHDIAQMRIA CRLASEVLDY ITPFVVAGVT TGELDRLCHE YMTHVQGTVP APLNYQPPGY PPFPKAICTS VNDVICHGIP GEKTLKNGDA LNIDITVIKN GYFGDTSRMF IIGEGSILAK RLVQTTYECM WLGIDQVRPG AHLGDIGHAI QKHAEAQGYS VVREYCGHGI GTVFHEDPQV VHYGRPGTGL ELKPGMIFTI EPMINAGKRD IRTMPDQWTV KTRDRSLSAQ WEHTILVTDT GHEVLT
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttgcgcaat tcaccgcatt tcaggcatcg actcatggct attacgctca aaaacgaaca cgacatcgcg cagatgcgca tcgcctgccg tctcgcgagc gaagtgctcg actacatcac gccattcgtc gtcgccggcg tgacgacggg cgaactcgat cgcctctgcc acgaatacat gacccatgtg cagggcacgg tgcccgcgcc gctcaactac cagccgcccg gctatccgcc gttcccgaag gcgatctgca cgtcggtcaa cgacgtgatc tgccacggca tcccgggcga gaagacgctc aagaacggcg acgcgttgaa catcgacatc accgtcatca agaacggcta tttcggcgac acgagccgga tgttcatcat cggcgagggc tcgatcctcg cgaagcgcct cgtgcaaacc acctacgaat gcatgtggct cggcatcgac caggtgaggc ccggcgcgca tctcggcgac atcggccacg cgatccagaa gcatgcggaa gcgcaaggct acagcgtcgt gcgcgaatac tgcggtcacg gcatcggcac ggtgttccac gaggacccgc aagtcgtcca ctacgggcgc ccgggcacgg gcctcgagct caagccgggc atgatcttca cgatcgagcc gatgatcaac gccggcaagc gcgacatccg cacgatgccc gaccagtgga ccgtcaagac ccgcgaccgc agcctgtccg cgcagtggga gcacacgatc ctcgtcaccg acacgggtca cgaggtgttg acgaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for BupsA.00039.b.A3.GE27936
HARVESTED ON: 11/24/2009
SEQUENCED ON: 1/13/2010
EXPECTED MW: 33kDa
OBSERVED MW: 33kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMLRNSPHFR HRLMAITLKN EHDIAQMRIA CRLASEVLDY ITPFVVAGVT TGELDRLCHE YMTHVQGTVP APLNYQPPGY PPFPKAICTS VNDVICHGIP GEKTLKNGDA LNIDITVIKN GYFGDTSRMF IIGEGSILAK RLVQTTYECM WLGIDQVRPG AHLGDIGHAI QKHAEAQGYS VVREYCGHGI GTVFHEDPQV VHYGRPGTGL ELKPGMIFTI EPMINAGKRD IRTMPDQWTV KTRDRSLSAQ WEHTILVTDT GHEVLTVSAG TPARPTFAA
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgttgc gcaattcacc gcatttcagg catcgactca tggctattac gctcaaaaac gaacacgaca tcgcgcagat gcgcatcgcc tgccgtctcg cgagcgaagt gctcgactac atcacgccat tcgtcgtcgc cggcgtgacg acgggcgaac tcgatcgcct ctgccacgaa tacatgaccc atgtgcaggg cacggtgccc gcgccgctca actaccagcc gcccggctat ccgccgttcc cgaaggcgat ctgcacgtcg gtcaacgacg tgatctgcca cggcatcccg ggcgagaaga cgctcaagaa cggcgacgcg ttgaacatcg acatcaccgt catcaagaac ggctatttcg gcgacacgag ccggatgttc atcatcggcg agggctcgat cctcgcgaag cgcctcgtgc aaaccaccta cgaatgcatg tggctcggca tcgaccaggt gaggcccggc gcgcatctcg gcgacatcgg ccacgcgatc cagaagcatg cggaagcgca aggctacagc gtcgtgcgcg aatactgcgg tcacggcatc ggcacggtgt tccacgagga cccgcaagtc gtccactacg ggcgcccggg cacgggcctc gagctcaagc cgggcatgat cttcacgatc gagccgatga tcaacgccgg caagcgcgac atccgcacga tgcccgacca gtggaccgtc aagacccgcg accgcagcct gtccgcgcag tgggagcaca cgatcctcgt caccgacacg ggtcacgagg tgttgacggt gtcggccggc acgccggcgc gcccgacgtt cgccgcgtaa taa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLRNSPHFR HRLMAITLKN EHDIAQMRIA CRLASEVLDY ITPFVVAGVT TGELDRLCHE YMTHVQGTVP APLNYQPPGY PPFPKAICTS VNDVICHGIP GEKTLKNGDA LNIDITVIKN GYFGDTSRMF IIGEGSILAK RLVQTTYECM WLGIDQVRPG AHLGDIGHAI QKHAEAQGYS VVREYCGHGI GTVFHEDPQV VHYGRPGTGL ELKPGMIFTI EPMINAGKRD IRTMPDQWTV KTRDRSLSAQ WEHTILVTDT GHEVLTVSAG TPARPTFAA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttgcgcaat tcaccgcatt tcaggcatcg actcatggct attacgctca aaaacgaaca cgacatcgcg cagatgcgca tcgcctgccg tctcgcgagc gaagtgctcg actacatcac gccattcgtc gtcgccggcg tgacgacggg cgaactcgat cgcctctgcc acgaatacat gacccatgtg cagggcacgg tgcccgcgcc gctcaactac cagccgcccg gctatccgcc gttcccgaag gcgatctgca cgtcggtcaa cgacgtgatc tgccacggca tcccgggcga gaagacgctc aagaacggcg acgcgttgaa catcgacatc accgtcatca agaacggcta tttcggcgac acgagccgga tgttcatcat cggcgagggc tcgatcctcg cgaagcgcct cgtgcaaacc acctacgaat gcatgtggct cggcatcgac caggtgaggc ccggcgcgca tctcggcgac atcggccacg cgatccagaa gcatgcggaa gcgcaaggct acagcgtcgt gcgcgaatac tgcggtcacg gcatcggcac ggtgttccac gaggacccgc aagtcgtcca ctacgggcgc ccgggcacgg gcctcgagct caagccgggc atgatcttca cgatcgagcc gatgatcaac gccggcaagc gcgacatccg cacgatgccc gaccagtgga ccgtcaagac ccgcgaccgc agcctgtccg cgcagtggga gcacacgatc ctcgtcaccg acacgggtca cgaggtgttg acggtgtcgg ccggcacgcc ggcgcgcccg acgttcgccg cgaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa
Details for BupsA.00039.b.A5.GE27938
HARVESTED ON: 11/24/2009
SEQUENCED ON: 12/3/2009
EXPECTED MW: 30kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 92
Validated AA Sequence
MTLKNEHDIA QMRIACRLAS EVLDYITPFV VAGVTTGELD RLCHEYMTHV QGTVPAPLNY QPPGYPPFPK AICTSVNDVI CHGIPGEKTL KNGDALNIDI TVIKNGYFGD TSRMFIIGEG SILAKRLVQT TYECMWLGID QVRPGAHLGD IGHAIQKHAE AQGYSVVREY CGHGIGTVFH EDPQVVHYGR PGTGLELKPG MIFTIEPMIN AGKRDIRTMP DQWTVKTRDR SLSAQWEHTI LVTDTGHEVL T
Validated NT Sequence
gggtaccctg gaagctcaga cccagggtcc tggttcgatg acgctcaaaa acgaacacga catcgcgcag atgcgcatcg cctgccgtct cgcgagcgaa gtgctcgact acatcacgcc attcgtcgtc gccggcgtga cgacgggcga actcgatcgc ctctgccacg aatacatgac ccatgtgcag ggcacggtgc ccgcgccgct caactaccag ccgcccggct atccgccgtt cccgaaggcg atctgcacgt cggtcaacga cgtgatctgc cacggcatcc cgggcgagaa gacgctcaag aacggcgacg cgttgaacat cgacatcacc gtcatcaaga acggctattt cggcgacacg agccggatgt tcatcatcgg cgagggctcg atcctcgcga agcgcctcgt gcaaaccacc tacgaatgca tgtggctcgg catcgaccag gtgaggcccg gcgcgcatct cggcgacatc ggccacgcga tccagaagca tgcggaagcg caaggctaca gcgtcgtgcg cgaatactgc ggtcacggca tcggcacggt gttccacgag gacccgcaag tcgtccacta cgggcgcccg ggcacgggcc tcgagctcaa gccgggcatg atcttcacga tcgagccgat gatcaacgcc ggcaagcgcg acatccgcac gatgcccgac cagtggaccg tcaagacccg cgaccgcagc ctgtccgcgc agtgggagca cacgatcctc gtcaccgaca cgggtcacga ggtgttgacg taataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTLKNEHDI AQMRIACRLA SEVLDYITPF VVAGVTTGEL DRLCHEYMTH VQGTVPAPLN YQPPGYPPFP KAICTSVNDV ICHGIPGEKT LKNGDALNID ITVIKNGYFG DTSRMFIIGE GSILAKRLVQ TTYECMWLGI DQVRPGAHLG DIGHAIQKHA EAQGYSVVRE YCGHGIGTVF HEDPQVVHYG RPGTGLELKP GMIFTIEPMI NAGKRDIRTM PDQWTVKTRD RSLSAQWEHT ILVTDTGHEV LT
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacgctcaaa aacgaacacg acatcgcgca gatgcgcatc gcctgccgtc tcgcgagcga agtgctcgac tacatcacgc cattcgtcgt cgccggcgtg acgacgggcg aactcgatcg cctctgccac gaatacatga cccatgtgca gggcacggtg cccgcgccgc tcaactacca gccgcccggc tatccgccgt tcccgaaggc gatctgcacg tcggtcaacg acgtgatctg ccacggcatc ccgggcgaga agacgctcaa gaacggcgac gcgttgaaca tcgacatcac cgtcatcaag aacggctatt tcggcgacac gagccggatg ttcatcatcg gcgagggctc gatcctcgcg aagcgcctcg tgcaaaccac ctacgaatgc atgtggctcg gcatcgacca ggtgaggccc ggcgcgcatc tcggcgacat cggccacgcg atccagaagc atgcggaagc gcaaggctac agcgtcgtgc gcgaatactg cggtcacggc atcggcacgg tgttccacga ggacccgcaa gtcgtccact acgggcgccc gggcacgggc ctcgagctca agccgggcat gatcttcacg atcgagccga tgatcaacgc cggcaagcgc gacatccgca cgatgcccga ccagtggacc gtcaagaccc gcgaccgcag cctgtccgcg cagtgggagc acacgatcct cgtcaccgac acgggtcacg aggtgttgac gaaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa
Details for BupsA.00039.b.A3.PW27883
PURIFICATION DATe: 2/16/2010
CONCENTRATION: 22.15mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 19
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMLRNSPHFR HRLMAITLKN EHDIAQMRIA CRLASEVLDY ITPFVVAGVT TGELDRLCHE YMTHVQGTVP APLNYQPPGY PPFPKAICTS VNDVICHGIP GEKTLKNGDA LNIDITVIKN GYFGDTSRMF IIGEGSILAK RLVQTTYECM WLGIDQVRPG AHLGDIGHAI QKHAEAQGYS VVREYCGHGI GTVFHEDPQV VHYGRPGTGL ELKPGMIFTI EPMINAGKRD IRTMPDQWTV KTRDRSLSAQ WEHTILVTDT GHEVLTVSAG TPARPTFAA
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgttgc gcaattcacc gcatttcagg catcgactca tggctattac gctcaaaaac gaacacgaca tcgcgcagat gcgcatcgcc tgccgtctcg cgagcgaagt gctcgactac atcacgccat tcgtcgtcgc cggcgtgacg acgggcgaac tcgatcgcct ctgccacgaa tacatgaccc atgtgcaggg cacggtgccc gcgccgctca actaccagcc gcccggctat ccgccgttcc cgaaggcgat ctgcacgtcg gtcaacgacg tgatctgcca cggcatcccg ggcgagaaga cgctcaagaa cggcgacgcg ttgaacatcg acatcaccgt catcaagaac ggctatttcg gcgacacgag ccggatgttc atcatcggcg agggctcgat cctcgcgaag cgcctcgtgc aaaccaccta cgaatgcatg tggctcggca tcgaccaggt gaggcccggc gcgcatctcg gcgacatcgg ccacgcgatc cagaagcatg cggaagcgca aggctacagc gtcgtgcgcg aatactgcgg tcacggcatc ggcacggtgt tccacgagga cccgcaagtc gtccactacg ggcgcccggg cacgggcctc gagctcaagc cgggcatgat cttcacgatc gagccgatga tcaacgccgg caagcgcgac atccgcacga tgcccgacca gtggaccgtc aagacccgcg accgcagcct gtccgcgcag tgggagcaca cgatcctcgt caccgacacg ggtcacgagg tgttgacggt gtcggccggc acgccggcgc gcccgacgtt cgccgcgtaa taa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLRNSPHFR HRLMAITLKN EHDIAQMRIA CRLASEVLDY ITPFVVAGVT TGELDRLCHE YMTHVQGTVP APLNYQPPGY PPFPKAICTS VNDVICHGIP GEKTLKNGDA LNIDITVIKN GYFGDTSRMF IIGEGSILAK RLVQTTYECM WLGIDQVRPG AHLGDIGHAI QKHAEAQGYS VVREYCGHGI GTVFHEDPQV VHYGRPGTGL ELKPGMIFTI EPMINAGKRD IRTMPDQWTV KTRDRSLSAQ WEHTILVTDT GHEVLTVSAG TPARPTFAA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTTGCGCAAT TCACCGCATT TCAGGCATCG ACTCATGGCT ATTACGCTCA AAAACGAACA CGACATCGCG CAGATGCGCA TCGCCTGCCG TCTCGCGAGC GAAGTGCTCG ACTACATCAC GCCATTCGTC GTCGCCGGCG TGACGACGGG CGAACTCGAT CGCCTCTGCC ACGAATACAT GACCCATGTG CAGGGCACGG TGCCCGCGCC GCTCAACTAC CAGCCGCCCG GCTATCCGCC GTTCCCGAAG GCGATCTGCA CGTCGGTCAA CGACGTGATC TGCCACGGCA TCCCGGGCGA GAAGACGCTC AAGAACGGCG ACGCGTTGAA CATCGACATC ACCGTCATCA AGAACGGCTA TTTCGGCGAC ACGAGCCGGA TGTTCATCAT CGGCGAGGGC TCGATCCTCG CGAAGCGCCT CGTGCAAACC ACCTACGAAT GCATGTGGCT CGGCATCGAC CAGGTGAGGC CCGGCGCGCA TCTCGGCGAC ATCGGCCACG CGATCCAGAA GCATGCGGAA GCGCAAGGCT ACAGCGTCGT GCGCGAATAC TGCGGTCACG GCATCGGCAC GGTGTTCCAC GAGGACCCGC AAGTCGTCCA CTACGGGCGC CCGGGCACGG GCCTCGAGCT CAAGCCGGGC ATGATCTTCA CGATCGAGCC GATGATCAAC GCCGGCAAGC GCGACATCCG CACGATGCCC GACCAGTGGA CCGTCAAGAC CCGCGACCGC AGCCTGTCCG CGCAGTGGGA GCACACGATC CTCGTCACCG ACACGGGTCA CGAGGTGTTG ACGGTGTCGG CCGGCACGCC GGCGCGCCCG ACGTTCGCCG CGAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA
Details for BupsA.00039.b.A5.PW27885
PURIFICATION DATe: 2/16/2010
CONCENTRATION: 27.58mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 21
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 92
Protocol Notes
notes unavailable
Validated AA Sequence
MTLKNEHDIA QMRIACRLAS EVLDYITPFV VAGVTTGELD RLCHEYMTHV QGTVPAPLNY QPPGYPPFPK AICTSVNDVI CHGIPGEKTL KNGDALNIDI TVIKNGYFGD TSRMFIIGEG SILAKRLVQT TYECMWLGID QVRPGAHLGD IGHAIQKHAE AQGYSVVREY CGHGIGTVFH EDPQVVHYGR PGTGLELKPG MIFTIEPMIN AGKRDIRTMP DQWTVKTRDR SLSAQWEHTI LVTDTGHEVL T
Validated NT Sequence
gggtaccctg gaagctcaga cccagggtcc tggttcgatg acgctcaaaa acgaacacga catcgcgcag atgcgcatcg cctgccgtct cgcgagcgaa gtgctcgact acatcacgcc attcgtcgtc gccggcgtga cgacgggcga actcgatcgc ctctgccacg aatacatgac ccatgtgcag ggcacggtgc ccgcgccgct caactaccag ccgcccggct atccgccgtt cccgaaggcg atctgcacgt cggtcaacga cgtgatctgc cacggcatcc cgggcgagaa gacgctcaag aacggcgacg cgttgaacat cgacatcacc gtcatcaaga acggctattt cggcgacacg agccggatgt tcatcatcgg cgagggctcg atcctcgcga agcgcctcgt gcaaaccacc tacgaatgca tgtggctcgg catcgaccag gtgaggcccg gcgcgcatct cggcgacatc ggccacgcga tccagaagca tgcggaagcg caaggctaca gcgtcgtgcg cgaatactgc ggtcacggca tcggcacggt gttccacgag gacccgcaag tcgtccacta cgggcgcccg ggcacgggcc tcgagctcaa gccgggcatg atcttcacga tcgagccgat gatcaacgcc ggcaagcgcg acatccgcac gatgcccgac cagtggaccg tcaagacccg cgaccgcagc ctgtccgcgc agtgggagca cacgatcctc gtcaccgaca cgggtcacga ggtgttgacg taataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTLKNEHDI AQMRIACRLA SEVLDYITPF VVAGVTTGEL DRLCHEYMTH VQGTVPAPLN YQPPGYPPFP KAICTSVNDV ICHGIPGEKT LKNGDALNID ITVIKNGYFG DTSRMFIIGE GSILAKRLVQ TTYECMWLGI DQVRPGAHLG DIGHAIQKHA EAQGYSVVRE YCGHGIGTVF HEDPQVVHYG RPGTGLELKP GMIFTIEPMI NAGKRDIRTM PDQWTVKTRD RSLSAQWEHT ILVTDTGHEV LT
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACGCTCAAA AACGAACACG ACATCGCGCA GATGCGCATC GCCTGCCGTC TCGCGAGCGA AGTGCTCGAC TACATCACGC CATTCGTCGT CGCCGGCGTG ACGACGGGCG AACTCGATCG CCTCTGCCAC GAATACATGA CCCATGTGCA GGGCACGGTG CCCGCGCCGC TCAACTACCA GCCGCCCGGC TATCCGCCGT TCCCGAAGGC GATCTGCACG TCGGTCAACG ACGTGATCTG CCACGGCATC CCGGGCGAGA AGACGCTCAA GAACGGCGAC GCGTTGAACA TCGACATCAC CGTCATCAAG AACGGCTATT TCGGCGACAC GAGCCGGATG TTCATCATCG GCGAGGGCTC GATCCTCGCG AAGCGCCTCG TGCAAACCAC CTACGAATGC ATGTGGCTCG GCATCGACCA GGTGAGGCCC GGCGCGCATC TCGGCGACAT CGGCCACGCG ATCCAGAAGC ATGCGGAAGC GCAAGGCTAC AGCGTCGTGC GCGAATACTG CGGTCACGGC ATCGGCACGG TGTTCCACGA GGACCCGCAA GTCGTCCACT ACGGGCGCCC GGGCACGGGC CTCGAGCTCA AGCCGGGCAT GATCTTCACG ATCGAGCCGA TGATCAACGC CGGCAAGCGC GACATCCGCA CGATGCCCGA CCAGTGGACC GTCAAGACCC GCGACCGCAG CCTGTCCGCG CAGTGGGAGC ACACGATCCT CGTCACCGAC ACGGGTCACG AGGTGTTGAC GAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA