BupsA.00039.a

methionine aminopeptidase, type I

CENTER ID: BupsA.00039.a
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.00039.a.A3.GE27930 Created from Decode construct for feature BupsA.00039.a 1 252
BupsA.00039.a.A5.GE27932 Created from Decode construct for feature BupsA.00039.a 8 272
BupsA.00039.a.A6.GE27933 Created from Decode construct for feature BupsA.00039.a 8 252
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.00039.a.A5.PW27879 Created from Decode construct for feature BupsA.00039.a 8 272
BupsA.00039.a.A6.PW27880 Created from Decode construct for feature BupsA.00039.a 8 252
BupsA.00039.a.A3.PW28715 Created from Decode construct for feature BupsA.00039.a 1 252
BupsA.00039.a.A5.PW31351 Created from Decode construct for feature BupsA.00039.a 8 272

External Resources

RESOURCE REFERENCE ID
OrthoMCL: OG5_126721
PATRIC ID: fig|320372.6.peg.2768
RefSeq: YP_334031
UniProt: Q3JQX2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MARSRQVPIR SKAEIAMARR AGTMAAQVLS MIAPHVKAGV TTNALDRLCR EYIVDVLRAR PANIGYHGYP KTICASVNHV VCHGIPSDHA LQDGDIVNID VALDHDGWYG DTSRMYCVGE PAAPARRLVD ATYEAMMAGI AAVRPGATLG DVGHAIQSVA HREGFSIVRE YCGHGIGRIY HDEPQVLHYG RPGAGLRLAP GMIFTIEPML NAGRAETRQL ADGWTVVTQD RSWSAQWEHM VVVTEDGFDI LTPWPDGEPA RSSAATGAAG AA
NT Sequence
ATGGCGCGTT CGCGACAGGT TCCGATCCGC TCGAAAGCGG AGATCGCGAT GGCGCGCCGC GCCGGCACGA TGGCGGCGCA GGTGCTGAGC ATGATCGCGC CGCACGTGAA GGCCGGCGTG ACGACCAACG CGCTCGACCG GCTATGCCGC GAGTACATCG TCGACGTACT GCGCGCGCGG CCCGCGAACA TCGGCTATCA CGGTTATCCG AAGACGATCT GCGCTTCCGT GAACCACGTG GTGTGCCACG GCATTCCGTC CGATCACGCG CTGCAGGACG GCGACATCGT CAATATCGAC GTCGCGCTCG ACCACGACGG CTGGTATGGC GACACGAGCC GCATGTACTG CGTCGGCGAG CCGGCCGCGC CGGCGCGGCG TCTCGTCGAT GCGACCTACG AAGCGATGAT GGCGGGCATC GCGGCGGTGC GCCCGGGCGC GACGCTCGGC GACGTCGGCC ATGCGATCCA GAGCGTGGCG CATCGCGAGG GCTTCAGCAT CGTGCGCGAG TATTGCGGCC ACGGCATCGG CCGCATCTAT CACGACGAGC CGCAGGTGCT CCATTACGGC CGGCCCGGCG CGGGGCTGCG TCTCGCGCCG GGAATGATCT TCACGATCGA GCCGATGCTC AATGCGGGGC GCGCCGAAAC GCGGCAGCTC GCCGACGGCT GGACGGTCGT GACGCAGGAC CGCTCGTGGT CCGCGCAATG GGAGCATATG GTCGTCGTGA CGGAAGACGG CTTCGACATC CTGACGCCAT GGCCGGATGG CGAGCCGGCG CGCTCGTCGG CGGCGACGGG GGCGGCCGGC GCCGCC
Details for BupsA.00039.a.A3.GE27930
HARVESTED ON: 11/24/2009
SEQUENCED ON: 12/3/2009
EXPECTED MW: 29kDa
OBSERVED MW: 29kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMARSRQVPI RSKAEIAMAR RAGTMAAQVL SMIAPHVKAG VTTNALDRLC REYIVDVLRA RPANIGYHGY PKTICASVNH VVCHGIPSDH ALQDGDIVNI DVALDHDGWY GDTSRMYCVG EPAAPARRLV DATYEAMMAG IAAVRPGATL GDVGHAIQSV AHREGFSIVR EYCGHGIGRI YHDEPQVLHY GRPGAGLRLA PGMIFTIEPM LNAGRAETRQ LADGWTVVTQ DRSWSAQWEH MVVVTEDGFD ILT
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggcgc gttcgcgaca ggttccgatc cgctcgaaag cggagatcgc gatggcgcgc cgcgccggca cgatggcggc gcaggtgctg agcatgatcg cgccgcacgt gaaggccggc gtgacgacca acgcgctcga ccggctatgc cgcgagtaca tcgtcgacgt actgcgcgcg cggcccgcga acatcggcta tcacggttat ccgaagacga tctgcgcttc cgtgaaccac gtggtgtgcc acggcattcc gtccgatcac gcgctgcagg acggcgacat cgtcaatatc gacgtcgcgc tcgaccacga cggctggtat ggcgacacga gccgcatgta ctgcgtcggc gagccggccg cgccggcgcg gcgtctcgtc gatgcgacct acgaagcgat gatggcgggc atcgcggcgg tgcgcccggg cgcgacgctc ggcgacgtcg gccatgcgat ccagagcgtg gcgcatcgcg agggcttcag catcgtgcgc gagtattgcg gccacggcat cggccgcatc tatcacgacg agccgcaggt gctccattac ggccggcccg gcgcggggct gcgtctcgcg ccgggaatga tcttcacgat cgagccgatg ctcaatgcgg ggcgcgccga aacgcggcag ctcgccgacg gctggacggt cgtgacgcag gaccgctcgt ggtccgcgca atgggagcat atggtcgtcg tgacggaaga cggcttcgac atcctgacgt aataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMARSRQVPI RSKAEIAMAR RAGTMAAQVL SMIAPHVKAG VTTNALDRLC REYIVDVLRA RPANIGYHGY PKTICASVNH VVCHGIPSDH ALQDGDIVNI DVALDHDGWY GDTSRMYCVG EPAAPARRLV DATYEAMMAG IAAVRPGATL GDVGHAIQSV AHREGFSIVR EYCGHGIGRI YHDEPQVLHY GRPGAGLRLA PGMIFTIEPM LNAGRAETRQ LADGWTVVTQ DRSWSAQWEH MVVVTEDGFD ILT
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcgcgttcg cgacaggttc cgatccgctc gaaagcggag atcgcgatgg cgcgccgcgc cggcacgatg gcggcgcagg tgctgagcat gatcgcgccg cacgtgaagg ccggcgtgac gaccaacgcg ctcgaccggc tatgccgcga gtacatcgtc gacgtactgc gcgcgcggcc cgcgaacatc ggctatcacg gttatccgaa gacgatctgc gcttccgtga accacgtggt gtgccacggc attccgtccg atcacgcgct gcaggacggc gacatcgtca atatcgacgt cgcgctcgac cacgacggct ggtatggcga cacgagccgc atgtactgcg tcggcgagcc ggccgcgccg gcgcggcgtc tcgtcgatgc gacctacgaa gcgatgatgg cgggcatcgc ggcggtgcgc ccgggcgcga cgctcggcga cgtcggccat gcgatccaga gcgtggcgca tcgcgagggc ttcagcatcg tgcgcgagta ttgcggccac ggcatcggcc gcatctatca cgacgagccg caggtgctcc attacggccg gcccggcgcg gggctgcgtc tcgcgccggg aatgatcttc acgatcgagc cgatgctcaa tgcggggcgc gccgaaacgc ggcagctcgc cgacggctgg acggtcgtga cgcaggaccg ctcgtggtcc gcgcaatggg agcatatggt cgtcgtgacg gaagacggct tcgacatcct gacgaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for BupsA.00039.a.A5.GE27932
HARVESTED ON: 11/24/2009
SEQUENCED ON: 12/3/2009
EXPECTED MW: 30kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMPIRSKAEI AMARRAGTMA AQVLSMIAPH VKAGVTTNAL DRLCREYIVD VLRARPANIG YHGYPKTICA SVNHVVCHGI PSDHALQDGD IVNIDVALDH DGWYGDTSRM YCVGEPAAPA RRLVDATYEA MMAGIAAVRP GATLGDVGHA IQSVAHREGF SIVREYCGHG IGRIYHDEPQ VLHYGRPGAG LRLAPGMIFT IEPMLNAGRA ETRQLADGWT VVTQDRSWSA QWEHMVVVTE DGFDILTPWP DGEPARSSAA TGAAGAA
Validated NT Sequence
ttattaggcg gcgccggccg cccccgtcgc cgccgacgag cgcgccggct cgccatccgg ccatggcgtc aggatgtcga agccgtcttc cgtcacgacg accatatgct cccattgcgc ggaccacgag cggtcctgcg tcacgaccgt ccagccgtcg gcgagctgcc gcgtttcggc gcgccccgca ttgagcatcg gctcgatcgt gaagatcatt cccggcgcga gacgcagccc cgcgccgggc cggccgtaat ggagcacctg cggctcgtcg tgatagatgc ggccgatgcc gtggccgcaa tactcgcgca cgatgctgaa gccctcgcga tgcgccacgc tctggatcgc atggccgacg tcgccgagcg tcgcgcccgg gcgcaccgcc gcgatgcccg ccatcatcgc ttcgtaggtc gcatcgacga gacgccgcgc cggcgcggcc ggctcgccga cgcagtacat gcggctcgtg tcgccatacc agccgtcgtg gtcgagcgcg acgtcgatat tgacgatgtc gccgtcctgc agcgcgtgat cggacggaat gccgtggcac accacgtggt tcacggaagc gcagatcgtc ttcggataac cgtgatagcc gatgttcgcg ggccgcgcgc gcagtacgtc gacgatgtac tcgcggcata gccggtcgag cgcgttggtc gtcacgccgg ccttcacgtg cggcgcgatc atgctcagca cctgcgccgc catcgtgccg gcgcggcgcg ccatcgcgat ctccgctttc gagcggatcg gcatcgaacc aggaccctgg gtctgagctt ccagggtacc catatgatgg tgatggtgat gagccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMPIRSKAEI AMARRAGTMA AQVLSMIAPH VKAGVTTNAL DRLCREYIVD VLRARPANIG YHGYPKTICA SVNHVVCHGI PSDHALQDGD IVNIDVALDH DGWYGDTSRM YCVGEPAAPA RRLVDATYEA MMAGIAAVRP GATLGDVGHA IQSVAHREGF SIVREYCGHG IGRIYHDEPQ VLHYGRPGAG LRLAPGMIFT IEPMLNAGRA ETRQLADGWT VVTQDRSWSA QWEHMVVVTE DGFDILTPWP DGEPARSSAA TGAAGAA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gccgatccgc tcgaaagcgg agatcgcgat ggcgcgccgc gccggcacga tggcggcgca ggtgctgagc atgatcgcgc cgcacgtgaa ggccggcgtg acgaccaacg cgctcgaccg gctatgccgc gagtacatcg tcgacgtact gcgcgcgcgg cccgcgaaca tcggctatca cggttatccg aagacgatct gcgcttccgt gaaccacgtg gtgtgccacg gcattccgtc cgatcacgcg ctgcaggacg gcgacatcgt caatatcgac gtcgcgctcg accacgacgg ctggtatggc gacacgagcc gcatgtactg cgtcggcgag ccggccgcgc cggcgcggcg tctcgtcgat gcgacctacg aagcgatgat ggcgggcatc gcggcggtgc gcccgggcgc gacgctcggc gacgtcggcc atgcgatcca gagcgtggcg catcgcgagg gcttcagcat cgtgcgcgag tattgcggcc acggcatcgg ccgcatctat cacgacgagc cgcaggtgct ccattacggc cggcccggcg cggggctgcg tctcgcgccg ggaatgatct tcacgatcga gccgatgctc aatgcggggc gcgccgaaac gcggcagctc gccgacggct ggacggtcgt gacgcaggac cgctcgtggt ccgcgcaatg ggagcatatg gtcgtcgtga cggaagacgg cttcgacatc ctgacgccat ggccggatgg cgagccggcg cgctcgtcgg cggcgacggg ggcggccggc gccgccaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for BupsA.00039.a.A6.GE27933
HARVESTED ON: 11/24/2009
SEQUENCED ON: 12/3/2009
EXPECTED MW: 29kDa
OBSERVED MW: 28kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMPIRSKAEI AMARRAGTMA AQVLSMIAPH VKAGVTTNAL DRLCREYIVD VLRARPANIG YHGYPKTICA SVNHVVCHGI PSDHALQDGD IVNIDVALDH DGWYGDTSRM YCVGEPAAPA RRLVDATYEA MMAGIAAVRP GATLGDVGHA IQSVAHREGF SIVREYCGHG IGRIYHDEPQ VLHYGRPGAG LRLAPGMIFT IEPMLNAGRA ETRQLADGWT VVTQDRSWSA QWEHMVVVTE DGFDILT
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgccga tccgctcgaa agcggagatc gcgatggcgc gccgcgccgg cacgatggcg gcgcaggtgc tgagcatgat cgcgccgcac gtgaaggccg gcgtgacgac caacgcgctc gaccggctat gccgcgagta catcgtcgac gtactgcgcg cgcggcccgc gaacatcggc tatcacggtt atccgaagac gatctgcgct tccgtgaacc acgtggtgtg ccacggcatt ccgtccgatc acgcgctgca ggacggcgac atcgtcaata tcgacgtcgc gctcgaccac gacggctggt atggcgacac gagccgcatg tactgcgtcg gcgagccggc cgcgccggcg cggcgtctcg tcgatgcgac ctacgaagcg atgatggcgg gcatcgcggc ggtgcgcccg ggcgcgacgc tcggcgacgt cggccatgcg atccagagcg tggcgcatcg cgagggcttc agcatcgtgc gcgagtattg cggccacggc atcggccgca tctatcacga cgagccgcag gtgctccatt acggccggcc cggcgcgggg ctgcgtctcg cgccgggaat gatcttcacg atcgagccga tgctcaatgc ggggcgcgcc gaaacgcggc agctcgccga cggctggacg gtcgtgacgc aggaccgctc gtggtccgcg caatgggagc atatggtcgt cgtgacggaa gacggcttcg acatcctgac gtaataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMPIRSKAEI AMARRAGTMA AQVLSMIAPH VKAGVTTNAL DRLCREYIVD VLRARPANIG YHGYPKTICA SVNHVVCHGI PSDHALQDGD IVNIDVALDH DGWYGDTSRM YCVGEPAAPA RRLVDATYEA MMAGIAAVRP GATLGDVGHA IQSVAHREGF SIVREYCGHG IGRIYHDEPQ VLHYGRPGAG LRLAPGMIFT IEPMLNAGRA ETRQLADGWT VVTQDRSWSA QWEHMVVVTE DGFDILT
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gccgatccgc tcgaaagcgg agatcgcgat ggcgcgccgc gccggcacga tggcggcgca ggtgctgagc atgatcgcgc cgcacgtgaa ggccggcgtg acgaccaacg cgctcgaccg gctatgccgc gagtacatcg tcgacgtact gcgcgcgcgg cccgcgaaca tcggctatca cggttatccg aagacgatct gcgcttccgt gaaccacgtg gtgtgccacg gcattccgtc cgatcacgcg ctgcaggacg gcgacatcgt caatatcgac gtcgcgctcg accacgacgg ctggtatggc gacacgagcc gcatgtactg cgtcggcgag ccggccgcgc cggcgcggcg tctcgtcgat gcgacctacg aagcgatgat ggcgggcatc gcggcggtgc gcccgggcgc gacgctcggc gacgtcggcc atgcgatcca gagcgtggcg catcgcgagg gcttcagcat cgtgcgcgag tattgcggcc acggcatcgg ccgcatctat cacgacgagc cgcaggtgct ccattacggc cggcccggcg cggggctgcg tctcgcgccg ggaatgatct tcacgatcga gccgatgctc aatgcggggc gcgccgaaac gcggcagctc gccgacggct ggacggtcgt gacgcaggac cgctcgtggt ccgcgcaatg ggagcatatg gtcgtcgtga cggaagacgg cttcgacatc ctgacgaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for BupsA.00039.a.A5.PW27879
PURIFICATION DATe: 2/16/2010
CONCENTRATION: 29.59mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 9
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMPIRSKAEI AMARRAGTMA AQVLSMIAPH VKAGVTTNAL DRLCREYIVD VLRARPANIG YHGYPKTICA SVNHVVCHGI PSDHALQDGD IVNIDVALDH DGWYGDTSRM YCVGEPAAPA RRLVDATYEA MMAGIAAVRP GATLGDVGHA IQSVAHREGF SIVREYCGHG IGRIYHDEPQ VLHYGRPGAG LRLAPGMIFT IEPMLNAGRA ETRQLADGWT VVTQDRSWSA QWEHMVVVTE DGFDILTPWP DGEPARSSAA TGAAGAA
Validated NT Sequence
ttattaggcg gcgccggccg cccccgtcgc cgccgacgag cgcgccggct cgccatccgg ccatggcgtc aggatgtcga agccgtcttc cgtcacgacg accatatgct cccattgcgc ggaccacgag cggtcctgcg tcacgaccgt ccagccgtcg gcgagctgcc gcgtttcggc gcgccccgca ttgagcatcg gctcgatcgt gaagatcatt cccggcgcga gacgcagccc cgcgccgggc cggccgtaat ggagcacctg cggctcgtcg tgatagatgc ggccgatgcc gtggccgcaa tactcgcgca cgatgctgaa gccctcgcga tgcgccacgc tctggatcgc atggccgacg tcgccgagcg tcgcgcccgg gcgcaccgcc gcgatgcccg ccatcatcgc ttcgtaggtc gcatcgacga gacgccgcgc cggcgcggcc ggctcgccga cgcagtacat gcggctcgtg tcgccatacc agccgtcgtg gtcgagcgcg acgtcgatat tgacgatgtc gccgtcctgc agcgcgtgat cggacggaat gccgtggcac accacgtggt tcacggaagc gcagatcgtc ttcggataac cgtgatagcc gatgttcgcg ggccgcgcgc gcagtacgtc gacgatgtac tcgcggcata gccggtcgag cgcgttggtc gtcacgccgg ccttcacgtg cggcgcgatc atgctcagca cctgcgccgc catcgtgccg gcgcggcgcg ccatcgcgat ctccgctttc gagcggatcg gcatcgaacc aggaccctgg gtctgagctt ccagggtacc catatgatgg tgatggtgat gagccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMPIRSKAEI AMARRAGTMA AQVLSMIAPH VKAGVTTNAL DRLCREYIVD VLRARPANIG YHGYPKTICA SVNHVVCHGI PSDHALQDGD IVNIDVALDH DGWYGDTSRM YCVGEPAAPA RRLVDATYEA MMAGIAAVRP GATLGDVGHA IQSVAHREGF SIVREYCGHG IGRIYHDEPQ VLHYGRPGAG LRLAPGMIFT IEPMLNAGRA ETRQLADGWT VVTQDRSWSA QWEHMVVVTE DGFDILTPWP DGEPARSSAA TGAAGAA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCCGATCCGC TCGAAAGCGG AGATCGCGAT GGCGCGCCGC GCCGGCACGA TGGCGGCGCA GGTGCTGAGC ATGATCGCGC CGCACGTGAA GGCCGGCGTG ACGACCAACG CGCTCGACCG GCTATGCCGC GAGTACATCG TCGACGTACT GCGCGCGCGG CCCGCGAACA TCGGCTATCA CGGTTATCCG AAGACGATCT GCGCTTCCGT GAACCACGTG GTGTGCCACG GCATTCCGTC CGATCACGCG CTGCAGGACG GCGACATCGT CAATATCGAC GTCGCGCTCG ACCACGACGG CTGGTATGGC GACACGAGCC GCATGTACTG CGTCGGCGAG CCGGCCGCGC CGGCGCGGCG TCTCGTCGAT GCGACCTACG AAGCGATGAT GGCGGGCATC GCGGCGGTGC GCCCGGGCGC GACGCTCGGC GACGTCGGCC ATGCGATCCA GAGCGTGGCG CATCGCGAGG GCTTCAGCAT CGTGCGCGAG TATTGCGGCC ACGGCATCGG CCGCATCTAT CACGACGAGC CGCAGGTGCT CCATTACGGC CGGCCCGGCG CGGGGCTGCG TCTCGCGCCG GGAATGATCT TCACGATCGA GCCGATGCTC AATGCGGGGC GCGCCGAAAC GCGGCAGCTC GCCGACGGCT GGACGGTCGT GACGCAGGAC CGCTCGTGGT CCGCGCAATG GGAGCATATG GTCGTCGTGA CGGAAGACGG CTTCGACATC CTGACGCCAT GGCCGGATGG CGAGCCGGCG CGCTCGTCGG CGGCGACGGG GGCGGCCGGC GCCGCCAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA
Details for BupsA.00039.a.A6.PW27880
PURIFICATION DATe: 2/16/2010
CONCENTRATION: 29.74mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 9
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMPIRSKAEI AMARRAGTMA AQVLSMIAPH VKAGVTTNAL DRLCREYIVD VLRARPANIG YHGYPKTICA SVNHVVCHGI PSDHALQDGD IVNIDVALDH DGWYGDTSRM YCVGEPAAPA RRLVDATYEA MMAGIAAVRP GATLGDVGHA IQSVAHREGF SIVREYCGHG IGRIYHDEPQ VLHYGRPGAG LRLAPGMIFT IEPMLNAGRA ETRQLADGWT VVTQDRSWSA QWEHMVVVTE DGFDILT
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgccga tccgctcgaa agcggagatc gcgatggcgc gccgcgccgg cacgatggcg gcgcaggtgc tgagcatgat cgcgccgcac gtgaaggccg gcgtgacgac caacgcgctc gaccggctat gccgcgagta catcgtcgac gtactgcgcg cgcggcccgc gaacatcggc tatcacggtt atccgaagac gatctgcgct tccgtgaacc acgtggtgtg ccacggcatt ccgtccgatc acgcgctgca ggacggcgac atcgtcaata tcgacgtcgc gctcgaccac gacggctggt atggcgacac gagccgcatg tactgcgtcg gcgagccggc cgcgccggcg cggcgtctcg tcgatgcgac ctacgaagcg atgatggcgg gcatcgcggc ggtgcgcccg ggcgcgacgc tcggcgacgt cggccatgcg atccagagcg tggcgcatcg cgagggcttc agcatcgtgc gcgagtattg cggccacggc atcggccgca tctatcacga cgagccgcag gtgctccatt acggccggcc cggcgcgggg ctgcgtctcg cgccgggaat gatcttcacg atcgagccga tgctcaatgc ggggcgcgcc gaaacgcggc agctcgccga cggctggacg gtcgtgacgc aggaccgctc gtggtccgcg caatgggagc atatggtcgt cgtgacggaa gacggcttcg acatcctgac gtaataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMPIRSKAEI AMARRAGTMA AQVLSMIAPH VKAGVTTNAL DRLCREYIVD VLRARPANIG YHGYPKTICA SVNHVVCHGI PSDHALQDGD IVNIDVALDH DGWYGDTSRM YCVGEPAAPA RRLVDATYEA MMAGIAAVRP GATLGDVGHA IQSVAHREGF SIVREYCGHG IGRIYHDEPQ VLHYGRPGAG LRLAPGMIFT IEPMLNAGRA ETRQLADGWT VVTQDRSWSA QWEHMVVVTE DGFDILT
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCCGATCCGC TCGAAAGCGG AGATCGCGAT GGCGCGCCGC GCCGGCACGA TGGCGGCGCA GGTGCTGAGC ATGATCGCGC CGCACGTGAA GGCCGGCGTG ACGACCAACG CGCTCGACCG GCTATGCCGC GAGTACATCG TCGACGTACT GCGCGCGCGG CCCGCGAACA TCGGCTATCA CGGTTATCCG AAGACGATCT GCGCTTCCGT GAACCACGTG GTGTGCCACG GCATTCCGTC CGATCACGCG CTGCAGGACG GCGACATCGT CAATATCGAC GTCGCGCTCG ACCACGACGG CTGGTATGGC GACACGAGCC GCATGTACTG CGTCGGCGAG CCGGCCGCGC CGGCGCGGCG TCTCGTCGAT GCGACCTACG AAGCGATGAT GGCGGGCATC GCGGCGGTGC GCCCGGGCGC GACGCTCGGC GACGTCGGCC ATGCGATCCA GAGCGTGGCG CATCGCGAGG GCTTCAGCAT CGTGCGCGAG TATTGCGGCC ACGGCATCGG CCGCATCTAT CACGACGAGC CGCAGGTGCT CCATTACGGC CGGCCCGGCG CGGGGCTGCG TCTCGCGCCG GGAATGATCT TCACGATCGA GCCGATGCTC AATGCGGGGC GCGCCGAAAC GCGGCAGCTC GCCGACGGCT GGACGGTCGT GACGCAGGAC CGCTCGTGGT CCGCGCAATG GGAGCATATG GTCGTCGTGA CGGAAGACGG CTTCGACATC CTGACGAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA
Details for BupsA.00039.a.A3.PW28715
PURIFICATION DATe: 2/16/2010
CONCENTRATION: 22.84mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 6
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMARSRQVPI RSKAEIAMAR RAGTMAAQVL SMIAPHVKAG VTTNALDRLC REYIVDVLRA RPANIGYHGY PKTICASVNH VVCHGIPSDH ALQDGDIVNI DVALDHDGWY GDTSRMYCVG EPAAPARRLV DATYEAMMAG IAAVRPGATL GDVGHAIQSV AHREGFSIVR EYCGHGIGRI YHDEPQVLHY GRPGAGLRLA PGMIFTIEPM LNAGRAETRQ LADGWTVVTQ DRSWSAQWEH MVVVTEDGFD ILT
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggcgc gttcgcgaca ggttccgatc cgctcgaaag cggagatcgc gatggcgcgc cgcgccggca cgatggcggc gcaggtgctg agcatgatcg cgccgcacgt gaaggccggc gtgacgacca acgcgctcga ccggctatgc cgcgagtaca tcgtcgacgt actgcgcgcg cggcccgcga acatcggcta tcacggttat ccgaagacga tctgcgcttc cgtgaaccac gtggtgtgcc acggcattcc gtccgatcac gcgctgcagg acggcgacat cgtcaatatc gacgtcgcgc tcgaccacga cggctggtat ggcgacacga gccgcatgta ctgcgtcggc gagccggccg cgccggcgcg gcgtctcgtc gatgcgacct acgaagcgat gatggcgggc atcgcggcgg tgcgcccggg cgcgacgctc ggcgacgtcg gccatgcgat ccagagcgtg gcgcatcgcg agggcttcag catcgtgcgc gagtattgcg gccacggcat cggccgcatc tatcacgacg agccgcaggt gctccattac ggccggcccg gcgcggggct gcgtctcgcg ccgggaatga tcttcacgat cgagccgatg ctcaatgcgg ggcgcgccga aacgcggcag ctcgccgacg gctggacggt cgtgacgcag gaccgctcgt ggtccgcgca atgggagcat atggtcgtcg tgacggaaga cggcttcgac atcctgacgt aataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMARSRQVPI RSKAEIAMAR RAGTMAAQVL SMIAPHVKAG VTTNALDRLC REYIVDVLRA RPANIGYHGY PKTICASVNH VVCHGIPSDH ALQDGDIVNI DVALDHDGWY GDTSRMYCVG EPAAPARRLV DATYEAMMAG IAAVRPGATL GDVGHAIQSV AHREGFSIVR EYCGHGIGRI YHDEPQVLHY GRPGAGLRLA PGMIFTIEPM LNAGRAETRQ LADGWTVVTQ DRSWSAQWEH MVVVTEDGFD ILT
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCGCGTTCG CGACAGGTTC CGATCCGCTC GAAAGCGGAG ATCGCGATGG CGCGCCGCGC CGGCACGATG GCGGCGCAGG TGCTGAGCAT GATCGCGCCG CACGTGAAGG CCGGCGTGAC GACCAACGCG CTCGACCGGC TATGCCGCGA GTACATCGTC GACGTACTGC GCGCGCGGCC CGCGAACATC GGCTATCACG GTTATCCGAA GACGATCTGC GCTTCCGTGA ACCACGTGGT GTGCCACGGC ATTCCGTCCG ATCACGCGCT GCAGGACGGC GACATCGTCA ATATCGACGT CGCGCTCGAC CACGACGGCT GGTATGGCGA CACGAGCCGC ATGTACTGCG TCGGCGAGCC GGCCGCGCCG GCGCGGCGTC TCGTCGATGC GACCTACGAA GCGATGATGG CGGGCATCGC GGCGGTGCGC CCGGGCGCGA CGCTCGGCGA CGTCGGCCAT GCGATCCAGA GCGTGGCGCA TCGCGAGGGC TTCAGCATCG TGCGCGAGTA TTGCGGCCAC GGCATCGGCC GCATCTATCA CGACGAGCCG CAGGTGCTCC ATTACGGCCG GCCCGGCGCG GGGCTGCGTC TCGCGCCGGG AATGATCTTC ACGATCGAGC CGATGCTCAA TGCGGGGCGC GCCGAAACGC GGCAGCTCGC CGACGGCTGG ACGGTCGTGA CGCAGGACCG CTCGTGGTCC GCGCAATGGG AGCATATGGT CGTCGTGACG GAAGACGGCT TCGACATCCT GACGAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA
Details for BupsA.00039.a.A5.PW31351
PURIFICATION DATe: 1/14/2011
CONCENTRATION: 28.37mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 13
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMPIRSKAEI AMARRAGTMA AQVLSMIAPH VKAGVTTNAL DRLCREYIVD VLRARPANIG YHGYPKTICA SVNHVVCHGI PSDHALQDGD IVNIDVALDH DGWYGDTSRM YCVGEPAAPA RRLVDATYEA MMAGIAAVRP GATLGDVGHA IQSVAHREGF SIVREYCGHG IGRIYHDEPQ VLHYGRPGAG LRLAPGMIFT IEPMLNAGRA ETRQLADGWT VVTQDRSWSA QWEHMVVVTE DGFDILTPWP DGEPARSSAA TGAAGAA
Validated NT Sequence
ttattaggcg gcgccggccg cccccgtcgc cgccgacgag cgcgccggct cgccatccgg ccatggcgtc aggatgtcga agccgtcttc cgtcacgacg accatatgct cccattgcgc ggaccacgag cggtcctgcg tcacgaccgt ccagccgtcg gcgagctgcc gcgtttcggc gcgccccgca ttgagcatcg gctcgatcgt gaagatcatt cccggcgcga gacgcagccc cgcgccgggc cggccgtaat ggagcacctg cggctcgtcg tgatagatgc ggccgatgcc gtggccgcaa tactcgcgca cgatgctgaa gccctcgcga tgcgccacgc tctggatcgc atggccgacg tcgccgagcg tcgcgcccgg gcgcaccgcc gcgatgcccg ccatcatcgc ttcgtaggtc gcatcgacga gacgccgcgc cggcgcggcc ggctcgccga cgcagtacat gcggctcgtg tcgccatacc agccgtcgtg gtcgagcgcg acgtcgatat tgacgatgtc gccgtcctgc agcgcgtgat cggacggaat gccgtggcac accacgtggt tcacggaagc gcagatcgtc ttcggataac cgtgatagcc gatgttcgcg ggccgcgcgc gcagtacgtc gacgatgtac tcgcggcata gccggtcgag cgcgttggtc gtcacgccgg ccttcacgtg cggcgcgatc atgctcagca cctgcgccgc catcgtgccg gcgcggcgcg ccatcgcgat ctccgctttc gagcggatcg gcatcgaacc aggaccctgg gtctgagctt ccagggtacc catatgatgg tgatggtgat gagccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMPIRSKAEI AMARRAGTMA AQVLSMIAPH VKAGVTTNAL DRLCREYIVD VLRARPANIG YHGYPKTICA SVNHVVCHGI PSDHALQDGD IVNIDVALDH DGWYGDTSRM YCVGEPAAPA RRLVDATYEA MMAGIAAVRP GATLGDVGHA IQSVAHREGF SIVREYCGHG IGRIYHDEPQ VLHYGRPGAG LRLAPGMIFT IEPMLNAGRA ETRQLADGWT VVTQDRSWSA QWEHMVVVTE DGFDILTPWP DGEPARSSAA TGAAGAA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCCGATCCGC TCGAAAGCGG AGATCGCGAT GGCGCGCCGC GCCGGCACGA TGGCGGCGCA GGTGCTGAGC ATGATCGCGC CGCACGTGAA GGCCGGCGTG ACGACCAACG CGCTCGACCG GCTATGCCGC GAGTACATCG TCGACGTACT GCGCGCGCGG CCCGCGAACA TCGGCTATCA CGGTTATCCG AAGACGATCT GCGCTTCCGT GAACCACGTG GTGTGCCACG GCATTCCGTC CGATCACGCG CTGCAGGACG GCGACATCGT CAATATCGAC GTCGCGCTCG ACCACGACGG CTGGTATGGC GACACGAGCC GCATGTACTG CGTCGGCGAG CCGGCCGCGC CGGCGCGGCG TCTCGTCGAT GCGACCTACG AAGCGATGAT GGCGGGCATC GCGGCGGTGC GCCCGGGCGC GACGCTCGGC GACGTCGGCC ATGCGATCCA GAGCGTGGCG CATCGCGAGG GCTTCAGCAT CGTGCGCGAG TATTGCGGCC ACGGCATCGG CCGCATCTAT CACGACGAGC CGCAGGTGCT CCATTACGGC CGGCCCGGCG CGGGGCTGCG TCTCGCGCCG GGAATGATCT TCACGATCGA GCCGATGCTC AATGCGGGGC GCGCCGAAAC GCGGCAGCTC GCCGACGGCT GGACGGTCGT GACGCAGGAC CGCTCGTGGT CCGCGCAATG GGAGCATATG GTCGTCGTGA CGGAAGACGG CTTCGACATC CTGACGCCAT GGCCGGATGG CGAGCCGGCG CGCTCGTCGG CGGCGACGGG GGCGGCCGGC GCCGCCAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA