BupsA.00035.a
ribose-phosphate pyrophosphokinase
| CENTER ID: | BupsA.00035.a |
| ORGANISM: | Burkholderia pseudomallei 1710b |
| ASSOCIATED DISEASE: | Melioidosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BupsA.00035.a.A1.GE25009 | Full length( BupsA.00035.a ) | 1 | 318 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BupsA.00035.a.A1.PW25047 | Full length( BupsA.00035.a ) | 1 | 318 |
Structures
3DAH
DEPOSITED: 5/29/2008
DETERMINATION: XRay
CLONE: BupsA.00035.a.A1.GE25009
PROTEIN: BupsA.00035.a.A1.PW25047
Publications by SSGCID
Combining functional and structural genomics to sample the essential Burkholderia structome.
Abendroth J, Armour B, Barrett L, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Fox D, Gallagher LA, Gardberg AS, Gillespie A, Manoil C, Myler PJ, Nakazawa-Hewitt S, Napuli A, Nguyen MT, Patrapuvich R, Phan I, Stacy R, Staker BL, Stewart LJ, Van Voorhis WC
PLoS ONE - 2012
volume 8, issue 1, pages e53851
PMID: 23382856; PMCID: PMC3561365
PDB:
3D63,
3DAH,
3EIZ,
3EJ2,
3EK2,
3EZO,
3F0F,
3FTP,
3GK0,
3GK3,
3GVF,
3GWA,
3GWE,
3IML,
3SZ8,
3T4C,
3TML,
3TMQ,
3TXY,
3U7J,
3UE9,
3UK1,
3UK2,
3UND,
3UPT,
3URR,
3UW1,
3UW2,
3UW3,
3V2I,
3V7N,
3V8H,
3V9O,
3V9P,
3VAV,
4DDO,
4DFE,
4DHE,
4DHK,
4DUT,
4DZ4,
4E4T,
4E51,
4EFI,
4EG0,
4EGJ,
4EK2,
4EQY,
4EWG,
4EX5,
4EXQ,
4F2G,
4F32,
4F3N,
4F3Y,
4F4H,
4F7D,
4FK8,
4FRY,
4G1K,
4G67,
4G6Z,
4GHK,
4H3Y,
4H3Z,
4H4G
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_126730 |
| PATRIC ID: | fig|320372.6.peg.763 |
| RefSeq: | YP_332167 |
| UniProt: | Q3JW86 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSSHDGLMVF TGNANPALAQ EVVKILGIPL GKAMVSRFSD GEIQVEIQEN VRGKDVFVLQ STCAPTNDNL MELMIMVDAL KRASAGRITA AIPYFGYARQ DRRPRSARVA ISAKVVANML EIAGVERIIT MDLHADQIQG FFDIPVDNIY ATPILLGDLR KQNYPDLLVV SPDVGGVVRA RALAKQLNCD LAIIDKRRPK ANVAEVMNII GEVEGRTCVI MDDMVDTAGT LCKAAQVLKE RGAKQVFAYA THPVLSGGAA DRIAASALDE LVVTDTIPLS AESLACPKIR ALSSAGLLAE TFSRIRRGDS VMSLFAES
NT Sequence
ATGAGCAGCC ATGACGGCCT GATGGTTTTT ACTGGCAACG CCAATCCCGC GCTTGCTCAG GAAGTCGTCA AAATTCTGGG TATTCCCCTC GGCAAAGCAA TGGTCAGCCG TTTCTCCGAC GGCGAGATCC AAGTCGAAAT CCAGGAAAAC GTGCGCGGCA AGGACGTCTT CGTCCTGCAA TCGACGTGCG CGCCGACGAA CGACAACCTG ATGGAACTGA TGATCATGGT CGATGCGCTC AAGCGCGCAT CCGCCGGCCG GATCACCGCC GCCATCCCCT ACTTCGGCTA TGCGCGCCAG GATCGCCGCC CGCGTTCGGC GCGCGTCGCG ATCTCCGCGA AGGTCGTCGC GAACATGCTG GAAATCGCCG GCGTCGAGCG GATCATCACG ATGGATCTGC ACGCCGACCA GATTCAAGGC TTCTTCGACA TCCCCGTCGA CAACATCTAC GCGACGCCGA TCCTGCTGGG CGACCTGCGC AAGCAGAACT ACCCGGATCT GCTCGTCGTG TCGCCGGACG TCGGCGGCGT CGTGCGCGCC CGCGCGCTCG CGAAGCAGCT GAACTGCGAT CTCGCGATCA TCGACAAGCG CCGTCCGAAG GCGAATGTCG CCGAAGTGAT GAACATCATC GGTGAAGTCG AAGGCCGCAC CTGCGTGATC ATGGACGACA TGGTCGACAC GGCGGGCACG CTCTGCAAGG CCGCGCAAGT GCTCAAGGAG CGCGGCGCGA AGCAGGTGTT CGCGTATGCG ACGCACCCGG TGCTCTCGGG CGGCGCGGCC GACCGGATCG CCGCTTCGGC GCTCGACGAA CTTGTCGTCA CCGACACGAT TCCGCTGTCC GCCGAATCGC TCGCGTGCCC GAAGATCCGC GCGCTATCGA GCGCGGGCCT CCTCGCCGAG ACGTTCTCGC GGATCCGCCG CGGCGATTCG GTGATGTCGC TGTTCGCGGA ATCG
Details for BupsA.00035.a.A1.GE25009
| HARVESTED ON: | 2/12/2008 |
| SEQUENCED ON: | 7/29/2008 |
| EXPECTED MW: | 36kDa |
| OBSERVED MW: | 36kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 99 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSSHDGLMV FTGNANPALA QEVVKILGIP LGKAMVSRFS DGEIQVEIQE NVRGKDVFVL QSTCAPTNDN LMELMIMVDA LKRASAGRIT AAIPYFGYAR QDRRPRSARV AISAKVVANM LEIAGVERII TMDLHADQIQ GFFDIPVDNI YATPILLGDL RKQNYPDLLV VSPDVGGVVR ARALAKQLNC DLAIIDKRRP KANVAEVMNI IGEVEGRTCV IMDDMVDTAG TLCKAAQVLK ERGAKQVFAY ATHPVLSGGA ADRIAASALD ELVVTDTIPL SAESLACPKI RALSSAGLLA ETFSRIRRGD SVMSLFA
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgagca gccatgacgg cctgatggtt tttactggca acgccaatcc cgcgcttgct caggaagtcg tcaaaattct gggtattccc ctcggcaaag caatggtcag ccgtttctcc gacggcgaga tccaagtcga aatccaggaa aacgtgcgcg gcaaggacgt cttcgtcctg caatcgacgt gcgcgccgac gaacgacaac ctgatggaac tgatgatcat ggtcgatgcg ctcaagcgcg catccgccgg ccggatcacc gccgccatcc cctacttcgg ctatgcgcgc caggatcgcc gcccgcgttc ggcgcgcgtc gcgatctccg cgaaggtcgt cgcgaacatg ctggaaatcg ccggcgtcga gcggatcatc acgatggatc tgcacgccga ccagattcaa ggcttcttcg acatccccgt cgacaacatc tacgcgacgc cgatcctgct gggcgacctg cgcaagcaga actacccgga tctgctcgtc gtgtcgccgg acgtcggcgg cgtcgtgcgc gcccgcgcgc tcgcgaagca gctgaactgc gatctcgcga tcatcgacaa gcgccgtccg aaggcgaatg tcgccgaagt gatgaacatc atcggtgaag tcgaaggccg cacctgcgtg atcatggacg acatggtcga cacggcgggc acgctctgca aggccgcgca agtgctcaag gagcgcggcg cgaagcaggt gttcgcgtat gcgacgcacc cggtgctctc gggcggcgcg gccgaccgga tcgccgcttc ggcgctcgac gaacttgtcg tcaccgacac gattccgctg tccgccgaat cgctcgcgtg cccgaagatc cgcgcgctat cgagcgcggg cctcctcgcc gagacgttct cgcggatccg ccgcggcgat tcggtgatgt cgctgttcgc gg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSSHDGLMV FTGNANPALA QEVVKILGIP LGKAMVSRFS DGEIQVEIQE NVRGKDVFVL QSTCAPTNDN LMELMIMVDA LKRASAGRIT AAIPYFGYAR QDRRPRSARV AISAKVVANM LEIAGVERII TMDLHADQIQ GFFDIPVDNI YATPILLGDL RKQNYPDLLV VSPDVGGVVR ARALAKQLNC DLAIIDKRRP KANVAEVMNI IGEVEGRTCV IMDDMVDTAG TLCKAAQVLK ERGAKQVFAY ATHPVLSGGA ADRIAASALD ELVVTDTIPL SAESLACPKI RALSSAGLLA ETFSRIRRGD SVMSLFAES
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcagccat gacggcctga tggtttttac tggcaacgcc aatcccgcgc ttgctcagga agtcgtcaaa attctgggta ttcccctcgg caaagcaatg gtcagccgtt tctccgacgg cgagatccaa gtcgaaatcc aggaaaacgt gcgcggcaag gacgtcttcg tcctgcaatc gacgtgcgcg ccgacgaacg acaacctgat ggaactgatg atcatggtcg atgcgctcaa gcgcgcatcc gccggccgga tcaccgccgc catcccctac ttcggctatg cgcgccagga tcgccgcccg cgttcggcgc gcgtcgcgat ctccgcgaag gtcgtcgcga acatgctgga aatcgccggc gtcgagcgga tcatcacgat ggatctgcac gccgaccaga ttcaaggctt cttcgacatc cccgtcgaca acatctacgc gacgccgatc ctgctgggcg acctgcgcaa gcagaactac ccggatctgc tcgtcgtgtc gccggacgtc ggcggcgtcg tgcgcgcccg cgcgctcgcg aagcagctga actgcgatct cgcgatcatc gacaagcgcc gtccgaaggc gaatgtcgcc gaagtgatga acatcatcgg tgaagtcgaa ggccgcacct gcgtgatcat ggacgacatg gtcgacacgg cgggcacgct ctgcaaggcc gcgcaagtgc tcaaggagcg cggcgcgaag caggtgttcg cgtatgcgac gcacccggtg ctctcgggcg gcgcggccga ccggatcgcc gcttcggcgc tcgacgaact tgtcgtcacc gacacgattc cgctgtccgc cgaatcgctc gcgtgcccga agatccgcgc gctatcgagc gcgggcctcc tcgccgagac gttctcgcgg atccgccgcg gcgattcggt gatgtcgctg ttcgcggaat cgtaaaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for BupsA.00035.a.A1.PW25047
| PURIFICATION DATe: | 2/27/2008 |
| CONCENTRATION: | 13.2mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 10 |
| VIAL VOLUME: | 50µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 99 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSSHDGLMV FTGNANPALA QEVVKILGIP LGKAMVSRFS DGEIQVEIQE NVRGKDVFVL QSTCAPTNDN LMELMIMVDA LKRASAGRIT AAIPYFGYAR QDRRPRSARV AISAKVVANM LEIAGVERII TMDLHADQIQ GFFDIPVDNI YATPILLGDL RKQNYPDLLV VSPDVGGVVR ARALAKQLNC DLAIIDKRRP KANVAEVMNI IGEVEGRTCV IMDDMVDTAG TLCKAAQVLK ERGAKQVFAY ATHPVLSGGA ADRIAASALD ELVVTDTIPL SAESLACPKI RALSSAGLLA ETFSRIRRGD SVMSLFA
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgagca gccatgacgg cctgatggtt tttactggca acgccaatcc cgcgcttgct caggaagtcg tcaaaattct gggtattccc ctcggcaaag caatggtcag ccgtttctcc gacggcgaga tccaagtcga aatccaggaa aacgtgcgcg gcaaggacgt cttcgtcctg caatcgacgt gcgcgccgac gaacgacaac ctgatggaac tgatgatcat ggtcgatgcg ctcaagcgcg catccgccgg ccggatcacc gccgccatcc cctacttcgg ctatgcgcgc caggatcgcc gcccgcgttc ggcgcgcgtc gcgatctccg cgaaggtcgt cgcgaacatg ctggaaatcg ccggcgtcga gcggatcatc acgatggatc tgcacgccga ccagattcaa ggcttcttcg acatccccgt cgacaacatc tacgcgacgc cgatcctgct gggcgacctg cgcaagcaga actacccgga tctgctcgtc gtgtcgccgg acgtcggcgg cgtcgtgcgc gcccgcgcgc tcgcgaagca gctgaactgc gatctcgcga tcatcgacaa gcgccgtccg aaggcgaatg tcgccgaagt gatgaacatc atcggtgaag tcgaaggccg cacctgcgtg atcatggacg acatggtcga cacggcgggc acgctctgca aggccgcgca agtgctcaag gagcgcggcg cgaagcaggt gttcgcgtat gcgacgcacc cggtgctctc gggcggcgcg gccgaccgga tcgccgcttc ggcgctcgac gaacttgtcg tcaccgacac gattccgctg tccgccgaat cgctcgcgtg cccgaagatc cgcgcgctat cgagcgcggg cctcctcgcc gagacgttct cgcggatccg ccgcggcgat tcggtgatgt cgctgttcgc gg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSSHDGLMV FTGNANPALA QEVVKILGIP LGKAMVSRFS DGEIQVEIQE NVRGKDVFVL QSTCAPTNDN LMELMIMVDA LKRASAGRIT AAIPYFGYAR QDRRPRSARV AISAKVVANM LEIAGVERII TMDLHADQIQ GFFDIPVDNI YATPILLGDL RKQNYPDLLV VSPDVGGVVR ARALAKQLNC DLAIIDKRRP KANVAEVMNI IGEVEGRTCV IMDDMVDTAG TLCKAAQVLK ERGAKQVFAY ATHPVLSGGA ADRIAASALD ELVVTDTIPL SAESLACPKI RALSSAGLLA ETFSRIRRGD SVMSLFAES
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGCAGCCAT GACGGCCTGA TGGTTTTTAC TGGCAACGCC AATCCCGCGC TTGCTCAGGA AGTCGTCAAA ATTCTGGGTA TTCCCCTCGG CAAAGCAATG GTCAGCCGTT TCTCCGACGG CGAGATCCAA GTCGAAATCC AGGAAAACGT GCGCGGCAAG GACGTCTTCG TCCTGCAATC GACGTGCGCG CCGACGAACG ACAACCTGAT GGAACTGATG ATCATGGTCG ATGCGCTCAA GCGCGCATCC GCCGGCCGGA TCACCGCCGC CATCCCCTAC TTCGGCTATG CGCGCCAGGA TCGCCGCCCG CGTTCGGCGC GCGTCGCGAT CTCCGCGAAG GTCGTCGCGA ACATGCTGGA AATCGCCGGC GTCGAGCGGA TCATCACGAT GGATCTGCAC GCCGACCAGA TTCAAGGCTT CTTCGACATC CCCGTCGACA ACATCTACGC GACGCCGATC CTGCTGGGCG ACCTGCGCAA GCAGAACTAC CCGGATCTGC TCGTCGTGTC GCCGGACGTC GGCGGCGTCG TGCGCGCCCG CGCGCTCGCG AAGCAGCTGA ACTGCGATCT CGCGATCATC GACAAGCGCC GTCCGAAGGC GAATGTCGCC GAAGTGATGA ACATCATCGG TGAAGTCGAA GGCCGCACCT GCGTGATCAT GGACGACATG GTCGACACGG CGGGCACGCT CTGCAAGGCC GCGCAAGTGC TCAAGGAGCG CGGCGCGAAG CAGGTGTTCG CGTATGCGAC GCACCCGGTG CTCTCGGGCG GCGCGGCCGA CCGGATCGCC GCTTCGGCGC TCGACGAACT TGTCGTCACC GACACGATTC CGCTGTCCGC CGAATCGCTC GCGTGCCCGA AGATCCGCGC GCTATCGAGC GCGGGCCTCC TCGCCGAGAC GTTCTCGCGG ATCCGCCGCG GCGATTCGGT GATGTCGCTG TTCGCGGAAT CGTAAAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA