BupsA.00032.a
S-adenosyl-L-homocysteine hydrolase
| CENTER ID: | BupsA.00032.a |
| ORGANISM: | Burkholderia pseudomallei 1710b |
| ASSOCIATED DISEASE: | Melioidosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BupsA.00032.a.A1.GE25007 | Full length( BupsA.00032.a ) | 1 | 473 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BupsA.00032.a.A1.PW25045 | Full length( BupsA.00032.a ) | 1 | 473 |
Structures
3D64
DEPOSITED: 5/18/2008
DETERMINATION: XRay
CLONE: BupsA.00032.a.A1.GE25007
PROTEIN: BupsA.00032.a.A1.PW25045
3GLQ
DEPOSITED: 3/12/2009
DETERMINATION: XRay
CLONE: BupsA.00032.a.A1.GE25007
PROTEIN: BupsA.00032.a.A1.PW25045
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_127233 |
| PATRIC ID: | fig|320372.6.peg.57 |
| RefSeq: | YP_331474 |
| UniProt: | Q3JY79 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNAAVIDSHS AQDYVVADIA LAGWGRKELN IAETEMPGLV QIRDEYKAQQ PLKGARIAGS LHMTIQTGVL IETLKALGAD VRWASCNIFS TQDHAAAAIV EAGTPVFAFK GESLDEYWEF SHRIFEWPNG EFANMILDDG GDATLLLILG SKAEKDRSVI ARPTNEEEVA LFKSIERHLE IDGSWYSKRL AHIKGVTEET TTGVHRLYQM EKDGRLPFPA FNVNDSVTKS KFDNLYGCRE SLVDGIKRAT DVMIAGKIAV VAGYGDVGKG CAQSLRGLGA TVWVTEIDPI CALQAAMEGY RVVTMEYAAD KADIFVTATG NYHVINHDHM KAMRHNAIVC NIGHFDSEID VASTRQYQWE NIKPQVDHII FPDGKRVILL AEGRLVNLGC ATGHPSFVMS NSFTNQTLAQ IELFTRGGEY ANKVYVLPKH LDEKVARLHL ARIGAQLSEL SDDQAAYIGV SKAGPFKPDH YRY
NT Sequence
ATGAACGCCG CTGTCATCGA TTCCCATTCC GCACAAGATT ACGTCGTCGC CGATATCGCG CTGGCCGGCT GGGGCCGCAA GGAACTGAAC ATCGCCGAGA CCGAGATGCC CGGGCTCGTG CAGATCCGCG ACGAATACAA GGCGCAGCAG CCGCTGAAGG GCGCGCGCAT CGCCGGCTCG CTGCACATGA CGATCCAGAC GGGCGTGCTG ATCGAGACGC TGAAGGCGCT CGGCGCGGAC GTTCGCTGGG CGTCGTGCAA CATCTTCTCG ACGCAGGACC ACGCGGCCGC CGCGATCGTC GAGGCCGGCA CGCCCGTCTT CGCGTTCAAG GGCGAATCGC TCGACGAATA CTGGGAGTTC TCGCACCGCA TCTTCGAATG GCCGAACGGC GAGTTCGCGA ACATGATCCT TGATGACGGC GGCGACGCAA CGCTGCTGCT GATCCTCGGC TCGAAGGCCG AGAAGGACCG CTCGGTGATC GCCCGGCCGA CCAACGAGGA AGAAGTCGCG CTCTTCAAGT CGATCGAGCG CCACCTCGAA ATCGACGGCA GCTGGTACTC GAAGCGCCTC GCGCACATCA AGGGCGTGAC CGAAGAGACG ACGACGGGCG TGCACCGCCT GTACCAGATG GAAAAGGACG GCCGCCTGCC GTTCCCGGCG TTCAACGTCA ACGATTCCGT GACGAAATCG AAGTTCGACA ACCTGTACGG CTGCCGCGAG TCGCTCGTCG ACGGCATCAA GCGCGCGACC GACGTGATGA TCGCGGGCAA GATCGCGGTG GTCGCGGGCT ACGGCGACGT GGGCAAGGGC TGCGCGCAAT CGCTGCGCGG CCTGGGCGCG ACCGTGTGGG TGACCGAAAT CGATCCGATC TGCGCGCTGC AGGCGGCGAT GGAAGGCTAC CGCGTCGTGA CGATGGAATA CGCGGCCGAC AAGGCCGACA TCTTCGTGAC GGCGACCGGC AACTACCACG TGATCAACCA CGATCACATG AAGGCGATGC GCCATAACGC GATCGTCTGC AACATCGGTC ACTTCGATTC GGAAATCGAC GTCGCGTCGA CCCGCCAGTA CCAGTGGGAA AACATCAAGC CGCAGGTCGA CCACATCATC TTCCCGGACG GCAAGCGCGT GATCCTGCTG GCCGAAGGCC GCCTCGTGAA CCTCGGCTGC GCGACCGGCC ACCCGTCGTT CGTGATGTCG AACTCGTTCA CGAATCAGAC CCTCGCGCAG ATCGAGCTGT TCACGCGCGG CGGCGAGTAC GCCAACAAGG TGTACGTGCT GCCGAAGCAT CTCGACGAAA AGGTCGCGCG CCTGCACCTC GCGCGCATCG GCGCGCAGCT CTCCGAGCTG TCGGACGATC AGGCGGCGTA CATCGGCGTG TCGAAGGCGG GCCCGTTCAA GCCGGATCAC TATCGTTAC
Details for BupsA.00032.a.A1.GE25007
| HARVESTED ON: | 2/12/2008 |
| SEQUENCED ON: | 6/16/2009 |
| EXPECTED MW: | 54kDa |
| OBSERVED MW: | 54kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 89 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMNAAVIDSH SAQDYVVADI ALAGWGRKEL NIAETEMPGL VQIRDEYKAQ QPLKGARIAG SLHMTIQTGV LIETLKALGA DVRWASCNIF STQDHAAAAI VEAGTPVFAF KGESLDEYWE FSHRIFEWPN GEFANMILDD GGDATLLLIL GSKAEKDRSV IARPTNEEEV ALFKSIERHL EIDGSWYSKR LAHIKGVTEE TTTGVHRLYQ MEKDGRLPFP AFNVNDSVTK SKFDNLYGCR ESLVDXIKRA XXVMIAGKIA VVXGYXDXXK GCAXXLRGLG XXVWVTEXDX XCAXXXXMEX XXVVXMEYXX XXXDXFVXAX GXXHVIXHDH MKXMXHXXXV CNIGHFDXEX DVASTRQXQX ENIXXQVDHI IXPXGKRVIL LAEGRLXNLG CATGXPSFVM SNSFXNQTLA QIELFTRGGE YANKVYVXPX HLDEKVARLH LARIGAQLSE LSDDQAAYIG VSKAXPFKPD HYRY
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaacg ccgctgtcat cgattcccat tccgcacaag attacgtcgt cgccgatatc gcgctggccg gctggggccg caaggaactg aacatcgccg agaccgagat gcccgggctc gtgcagatcc gcgacgaata caaggcgcag cagccgctga agggcgcgcg catcgccggc tcgctgcaca tgacgatcca gacgggcgtg ctgatcgaga cgctgaaggc gctcggcgcg gacgttcgct gggcgtcgtg caacatcttc tcgacgcagg accacgcggc cgccgcgatc gtcgaggccg gcacgcccgt cttcgcgttc aagggcgaat cgctcgacga atactgggag ttctcgcacc gcatcttcga atggccgaac ggcgagttcg cgaacatgat ccttgatgac ggcggcgacg caacgctgct gctgatcctc ggctcgaagg ccgagaagga ccgctcggtg atcgcccggc cgaccaacga ggaagaagtc gcgctcttca agtcgatcga gcgccacctc gaaatcgacg gcagctggta ctcgaagcgc ctcgcgcaca tcaagggcgt gaccgaagag acgacgacgg gcgtgcaccg cctgtaccag atggaaaagg acggccgcct gccgttcccg gcgttcaacg tcaacgattc cgtgacgaaa tcgaagttcg acaacctgta cggctgccgc gagtcgctcg tcgacngcat caagcgcgcg ancgangtga tgatcgcggg caagatcgcg gtggtcncgg gctacngcga cntggnnaag ggctgcgcgc nancnctgcg cggnctgggn ncgnncgtgt gggtgaccga aatngatnnn atntgcgcnn nncannnggn gatggaagnn nnnngngtcg tgncgatgga atacncggnn gncaannncg acatnttcgt gncggcgncc ggcaantnnc acgtgatcan ncacgatcac atgaaggnga tgngccatna nncgatngtn tgcaacatcg gtcacttcga ttnggaaatn gacgtcgcgt cgacccgcca gtancagngg gaaaacatca anncgcaggt cgaccacatc atcttnccgg ncggcaagcg cgtgatcctg ctggccgaag gccgcctcgn gaacctnggn tgcgcgaccg gccncccgtc gttcgtgatg tcgaactcgt tcncgaatca gaccctcgcg cagatcgagc tgttcacgcg cggcggcgag tacgccaaca aggtgtacgt gcngccgaan catctngacg aaaaggtngc gcgcctgcac ctcgcgcgca tcggcgcgca gctctccgag ctgtcggacg atcaggcggc gtacatcggc gtgtcgaagg cggnnccgtt caagccggat cactatcgtt actaata
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMNAAVIDSH SAQDYVVADI ALAGWGRKEL NIAETEMPGL VQIRDEYKAQ QPLKGARIAG SLHMTIQTGV LIETLKALGA DVRWASCNIF STQDHAAAAI VEAGTPVFAF KGESLDEYWE FSHRIFEWPN GEFANMILDD GGDATLLLIL GSKAEKDRSV IARPTNEEEV ALFKSIERHL EIDGSWYSKR LAHIKGVTEE TTTGVHRLYQ MEKDGRLPFP AFNVNDSVTK SKFDNLYGCR ESLVDGIKRA TDVMIAGKIA VVAGYGDVGK GCAQSLRGLG ATVWVTEIDP ICALQAAMEG YRVVTMEYAA DKADIFVTAT GNYHVINHDH MKAMRHNAIV CNIGHFDSEI DVASTRQYQW ENIKPQVDHI IFPDGKRVIL LAEGRLVNLG CATGHPSFVM SNSFTNQTLA QIELFTRGGE YANKVYVLPK HLDEKVARLH LARIGAQLSE LSDDQAAYIG VSKAGPFKPD HYRY
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaacgccgct gtcatcgatt cccattccgc acaagattac gtcgtcgccg atatcgcgct ggccggctgg ggccgcaagg aactgaacat cgccgagacc gagatgcccg ggctcgtgca gatccgcgac gaatacaagg cgcagcagcc gctgaagggc gcgcgcatcg ccggctcgct gcacatgacg atccagacgg gcgtgctgat cgagacgctg aaggcgctcg gcgcggacgt tcgctgggcg tcgtgcaaca tcttctcgac gcaggaccac gcggccgccg cgatcgtcga ggccggcacg cccgtcttcg cgttcaaggg cgaatcgctc gacgaatact gggagttctc gcaccgcatc ttcgaatggc cgaacggcga gttcgcgaac atgatccttg atgacggcgg cgacgcaacg ctgctgctga tcctcggctc gaaggccgag aaggaccgct cggtgatcgc ccggccgacc aacgaggaag aagtcgcgct cttcaagtcg atcgagcgcc acctcgaaat cgacggcagc tggtactcga agcgcctcgc gcacatcaag ggcgtgaccg aagagacgac gacgggcgtg caccgcctgt accagatgga aaaggacggc cgcctgccgt tcccggcgtt caacgtcaac gattccgtga cgaaatcgaa gttcgacaac ctgtacggct gccgcgagtc gctcgtcgac ggcatcaagc gcgcgaccga cgtgatgatc gcgggcaaga tcgcggtggt cgcgggctac ggcgacgtgg gcaagggctg cgcgcaatcg ctgcgcggcc tgggcgcgac cgtgtgggtg accgaaatcg atccgatctg cgcgctgcag gcggcgatgg aaggctaccg cgtcgtgacg atggaatacg cggccgacaa ggccgacatc ttcgtgacgg cgaccggcaa ctaccacgtg atcaaccacg atcacatgaa ggcgatgcgc cataacgcga tcgtctgcaa catcggtcac ttcgattcgg aaatcgacgt cgcgtcgacc cgccagtacc agtgggaaaa catcaagccg caggtcgacc acatcatctt cccggacggc aagcgcgtga tcctgctggc cgaaggccgc ctcgtgaacc tcggctgcgc gaccggccac ccgtcgttcg tgatgtcgaa ctcgttcacg aatcagaccc tcgcgcagat cgagctgttc acgcgcggcg gcgagtacgc caacaaggtg tacgtgctgc cgaagcatct cgacgaaaag gtcgcgcgcc tgcacctcgc gcgcatcggc gcgcagctct ccgagctgtc ggacgatcag gcggcgtaca tcggcgtgtc gaaggcgggc ccgttcaagc cggatcacta tcgttactga aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for BupsA.00032.a.A1.PW25045
| PURIFICATION DATe: | 3/12/2008 |
| CONCENTRATION: | 21.5mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 43 |
| VIAL VOLUME: | 50µl |
| PERCENT IDENTITY: | 89 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMNAAVIDSH SAQDYVVADI ALAGWGRKEL NIAETEMPGL VQIRDEYKAQ QPLKGARIAG SLHMTIQTGV LIETLKALGA DVRWASCNIF STQDHAAAAI VEAGTPVFAF KGESLDEYWE FSHRIFEWPN GEFANMILDD GGDATLLLIL GSKAEKDRSV IARPTNEEEV ALFKSIERHL EIDGSWYSKR LAHIKGVTEE TTTGVHRLYQ MEKDGRLPFP AFNVNDSVTK SKFDNLYGCR ESLVDXIKRA XXVMIAGKIA VVXGYXDXXK GCAXXLRGLG XXVWVTEXDX XCAXXXXMEX XXVVXMEYXX XXXDXFVXAX GXXHVIXHDH MKXMXHXXXV CNIGHFDXEX DVASTRQXQX ENIXXQVDHI IXPXGKRVIL LAEGRLXNLG CATGXPSFVM SNSFXNQTLA QIELFTRGGE YANKVYVXPX HLDEKVARLH LARIGAQLSE LSDDQAAYIG VSKAXPFKPD HYRY
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaacg ccgctgtcat cgattcccat tccgcacaag attacgtcgt cgccgatatc gcgctggccg gctggggccg caaggaactg aacatcgccg agaccgagat gcccgggctc gtgcagatcc gcgacgaata caaggcgcag cagccgctga agggcgcgcg catcgccggc tcgctgcaca tgacgatcca gacgggcgtg ctgatcgaga cgctgaaggc gctcggcgcg gacgttcgct gggcgtcgtg caacatcttc tcgacgcagg accacgcggc cgccgcgatc gtcgaggccg gcacgcccgt cttcgcgttc aagggcgaat cgctcgacga atactgggag ttctcgcacc gcatcttcga atggccgaac ggcgagttcg cgaacatgat ccttgatgac ggcggcgacg caacgctgct gctgatcctc ggctcgaagg ccgagaagga ccgctcggtg atcgcccggc cgaccaacga ggaagaagtc gcgctcttca agtcgatcga gcgccacctc gaaatcgacg gcagctggta ctcgaagcgc ctcgcgcaca tcaagggcgt gaccgaagag acgacgacgg gcgtgcaccg cctgtaccag atggaaaagg acggccgcct gccgttcccg gcgttcaacg tcaacgattc cgtgacgaaa tcgaagttcg acaacctgta cggctgccgc gagtcgctcg tcgacngcat caagcgcgcg ancgangtga tgatcgcggg caagatcgcg gtggtcncgg gctacngcga cntggnnaag ggctgcgcgc nancnctgcg cggnctgggn ncgnncgtgt gggtgaccga aatngatnnn atntgcgcnn nncannnggn gatggaagnn nnnngngtcg tgncgatgga atacncggnn gncaannncg acatnttcgt gncggcgncc ggcaantnnc acgtgatcan ncacgatcac atgaaggnga tgngccatna nncgatngtn tgcaacatcg gtcacttcga ttnggaaatn gacgtcgcgt cgacccgcca gtancagngg gaaaacatca anncgcaggt cgaccacatc atcttnccgg ncggcaagcg cgtgatcctg ctggccgaag gccgcctcgn gaacctnggn tgcgcgaccg gccncccgtc gttcgtgatg tcgaactcgt tcncgaatca gaccctcgcg cagatcgagc tgttcacgcg cggcggcgag tacgccaaca aggtgtacgt gcngccgaan catctngacg aaaaggtngc gcgcctgcac ctcgcgcgca tcggcgcgca gctctccgag ctgtcggacg atcaggcggc gtacatcggc gtgtcgaagg cggnnccgtt caagccggat cactatcgtt actaata
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMNAAVIDSH SAQDYVVADI ALAGWGRKEL NIAETEMPGL VQIRDEYKAQ QPLKGARIAG SLHMTIQTGV LIETLKALGA DVRWASCNIF STQDHAAAAI VEAGTPVFAF KGESLDEYWE FSHRIFEWPN GEFANMILDD GGDATLLLIL GSKAEKDRSV IARPTNEEEV ALFKSIERHL EIDGSWYSKR LAHIKGVTEE TTTGVHRLYQ MEKDGRLPFP AFNVNDSVTK SKFDNLYGCR ESLVDGIKRA TDVMIAGKIA VVAGYGDVGK GCAQSLRGLG ATVWVTEIDP ICALQAAMEG YRVVTMEYAA DKADIFVTAT GNYHVINHDH MKAMRHNAIV CNIGHFDSEI DVASTRQYQW ENIKPQVDHI IFPDGKRVIL LAEGRLVNLG CATGHPSFVM SNSFTNQTLA QIELFTRGGE YANKVYVLPK HLDEKVARLH LARIGAQLSE LSDDQAAYIG VSKAGPFKPD HYRY
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAACGCCGCT GTCATCGATT CCCATTCCGC ACAAGATTAC GTCGTCGCCG ATATCGCGCT GGCCGGCTGG GGCCGCAAGG AACTGAACAT CGCCGAGACC GAGATGCCCG GGCTCGTGCA GATCCGCGAC GAATACAAGG CGCAGCAGCC GCTGAAGGGC GCGCGCATCG CCGGCTCGCT GCACATGACG ATCCAGACGG GCGTGCTGAT CGAGACGCTG AAGGCGCTCG GCGCGGACGT TCGCTGGGCG TCGTGCAACA TCTTCTCGAC GCAGGACCAC GCGGCCGCCG CGATCGTCGA GGCCGGCACG CCCGTCTTCG CGTTCAAGGG CGAATCGCTC GACGAATACT GGGAGTTCTC GCACCGCATC TTCGAATGGC CGAACGGCGA GTTCGCGAAC ATGATCCTTG ATGACGGCGG CGACGCAACG CTGCTGCTGA TCCTCGGCTC GAAGGCCGAG AAGGACCGCT CGGTGATCGC CCGGCCGACC AACGAGGAAG AAGTCGCGCT CTTCAAGTCG ATCGAGCGCC ACCTCGAAAT CGACGGCAGC TGGTACTCGA AGCGCCTCGC GCACATCAAG GGCGTGACCG AAGAGACGAC GACGGGCGTG CACCGCCTGT ACCAGATGGA AAAGGACGGC CGCCTGCCGT TCCCGGCGTT CAACGTCAAC GATTCCGTGA CGAAATCGAA GTTCGACAAC CTGTACGGCT GCCGCGAGTC GCTCGTCGAC GGCATCAAGC GCGCGACCGA CGTGATGATC GCGGGCAAGA TCGCGGTGGT CGCGGGCTAC GGCGACGTGG GCAAGGGCTG CGCGCAATCG CTGCGCGGCC TGGGCGCGAC CGTGTGGGTG ACCGAAATCG ATCCGATCTG CGCGCTGCAG GCGGCGATGG AAGGCTACCG CGTCGTGACG ATGGAATACG CGGCCGACAA GGCCGACATC TTCGTGACGG CGACCGGCAA CTACCACGTG ATCAACCACG ATCACATGAA GGCGATGCGC CATAACGCGA TCGTCTGCAA CATCGGTCAC TTCGATTCGG AAATCGACGT CGCGTCGACC CGCCAGTACC AGTGGGAAAA CATCAAGCCG CAGGTCGACC ACATCATCTT CCCGGACGGC AAGCGCGTGA TCCTGCTGGC CGAAGGCCGC CTCGTGAACC TCGGCTGCGC GACCGGCCAC CCGTCGTTCG TGATGTCGAA CTCGTTCACG AATCAGACCC TCGCGCAGAT CGAGCTGTTC ACGCGCGGCG GCGAGTACGC CAACAAGGTG TACGTGCTGC CGAAGCATCT CGACGAAAAG GTCGCGCGCC TGCACCTCGC GCGCATCGGC GCGCAGCTCT CCGAGCTGTC GGACGATCAG GCGGCGTACA TCGGCGTGTC GAAGGCGGGC CCGTTCAAGC CGGATCACTA TCGTTACTGA AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA