BupsA.00030.a

ATP-dependent protease ATP-binding subunit

CENTER ID: BupsA.00030.a
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: diffraction
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.00030.a.A2.GE27925 Created from Decode construct for feature BupsA.00030.a 1 448
BupsA.00030.a.A3.GE27926 Created from Decode construct for feature BupsA.00030.a 1 455
BupsA.00030.a.A4.GE27927 Created from Decode construct for feature BupsA.00030.a 108 466
BupsA.00030.a.A5.GE27928 Created from Decode construct for feature BupsA.00030.a 108 448
BupsA.00030.a.A6.GE27929 Created from Decode construct for feature BupsA.00030.a 108 455
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.00030.a.A4.PW27874 Created from Decode construct for feature BupsA.00030.a 108 466
BupsA.00030.a.A5.PW27875 Created from Decode construct for feature BupsA.00030.a 108 448
BupsA.00030.a.A6.PW27876 Created from Decode construct for feature BupsA.00030.a 108 455
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_128011
PATRIC ID: fig|320372.6.peg.2592
RefSeq: YP_333873
UniProt: Q3JRD0
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPATRPEHLR QKNIAAVSRV FTRSWCRGQT AYYVTHVSGG STFMADKKGS NSEKLLYCSF CGKSQHEVKK LIAGPSVFIC DECIDLCNEI IRDEAAAAGV EASLSKSDLP SPQEIRDILD QYVIGQERAK KILAVAVYNH YKRLKHLDKK DDVELSKSNI LLIGPTGSGK TLLAQTLARL LNVPFVIADA TTLTEAGYVG EDVENIIQKL LQNCNYEVEK AQRGIVYIDE IDKISRKSDN PSITRDVSGE GVQQALLKLV EGTMASVPPQ GGRKHPNQDF IQVDTTNILF ICGGAFDGLE KVITDRTEKT GIGFGATVKS KQERDAGEVL REVEPEDLIK FGLIPELIGR LPVVATLGKL DEAALMKILV EPKNALVKQY QKLFAMERVE LEIRPDALQA VARKAIRRKT GARGLRSIIE QALLDVMYEL PTLKGVSKVI IDDNVIEGDG KPLLIYEDTP KVAGSN
NT Sequence
GTGCCCGCCA CACGCCCCGA GCATCTGCGG CAAAAGAACA TCGCCGCCGT CTCCCGAGTA TTTACCAGGT CATGGTGTCG GGGACAAACG GCGTATTATG TAACTCATGT GTCCGGAGGC TCTACATTCA TGGCGGACAA GAAAGGTTCG AACAGCGAGA AGCTGTTGTA TTGCTCGTTT TGCGGCAAAA GCCAGCATGA GGTGAAGAAG CTCATCGCGG GGCCGTCGGT ATTCATCTGC GATGAATGTA TCGACTTGTG CAACGAGATC ATCCGCGACG AAGCGGCCGC GGCGGGCGTC GAAGCCAGCC TGTCCAAATC CGACCTGCCG AGCCCGCAGG AGATTCGCGA CATCCTCGAT CAGTACGTGA TCGGCCAGGA GCGCGCGAAG AAGATCCTCG CCGTCGCCGT CTACAATCAC TACAAGCGCC TGAAGCATCT CGACAAGAAG GACGATGTCG AGTTGTCGAA GAGCAACATC CTGTTGATCG GCCCGACGGG CTCCGGCAAG ACGCTGCTCG CGCAGACCCT CGCGCGCCTG CTGAACGTGC CGTTCGTGAT CGCCGACGCG ACCACGCTGA CGGAGGCCGG TTATGTCGGC GAGGACGTCG AGAACATCAT CCAGAAGCTG CTGCAGAACT GCAACTACGA GGTCGAGAAG GCGCAGCGCG GGATCGTCTA CATCGACGAA ATCGACAAGA TCAGCCGCAA GTCGGACAAC CCGTCGATCA CCCGCGACGT GTCGGGCGAG GGCGTCCAGC AGGCGCTGCT CAAGCTCGTC GAGGGCACGA TGGCGTCGGT GCCGCCGCAG GGCGGCCGCA AGCACCCGAA CCAGGATTTC ATCCAGGTCG ACACCACCAA CATCCTGTTC ATTTGCGGCG GCGCGTTCGA CGGCCTCGAG AAGGTGATCA CCGACCGCAC CGAGAAGACC GGCATCGGTT TCGGCGCGAC GGTCAAGAGC AAGCAGGAGC GGGACGCGGG CGAAGTGCTG CGCGAGGTCG AGCCGGAAGA CCTGATCAAA TTCGGGTTGA TCCCCGAGCT GATCGGCCGT CTGCCGGTGG TCGCGACGCT CGGCAAGCTC GATGAAGCCG CGCTGATGAA GATTCTCGTC GAGCCGAAGA ACGCGCTCGT CAAGCAGTAT CAGAAGCTGT TCGCGATGGA GCGGGTCGAA CTCGAGATTC GTCCGGACGC GCTGCAAGCC GTCGCCCGCA AGGCGATCCG CCGCAAGACG GGCGCGCGCG GGCTGCGTTC GATCATCGAG CAGGCGCTCC TCGACGTGAT GTACGAACTG CCGACGCTCA AGGGCGTCAG CAAGGTGATC ATCGACGACA ATGTCATCGA AGGAGACGGC AAGCCGTTAT TGATCTATGA GGACACGCCG AAAGTGGCGG GTTCGAAT
Details for BupsA.00030.a.A2.GE27925
HARVESTED ON: 11/24/2009
SEQUENCED ON: 12/3/2009
EXPECTED MW: 51kDa
OBSERVED MW: 50kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 39
Validated AA Sequence
MASVPPQGGR KHPNQDFIQV DTTNILFICG GAFDGLEKVI TDRTEKTGIG FGATVKSKQE RDAGEVLREV EPEDLIKFGL IPELIGRLPV VATLGKLDEA ALMKILVEPK NALVKQYQKL FAMERVELEI RPDALQAVAR KAIRRKTGAR GLRSIIEQAL LDVMYELPTL KGVSKVIIDD NVIEG
Validated NT Sequence
tcgggctttg ttagcagccg gatcctcgag aagcttggct gcagaacttg ttcgtgctgt ttattatcct tcgatgacat tgtcgtcgat gatcaccttg ctgacgccct tgagcgtcgg cagttcgtac atcacgtcga ggagcgcctg ctcgatgatc gaacgcagcc cgcgcgcgcc cgtcttgcgg cggatcgcct tgcgggcgac ggcttgcagc gcgtccggac gaatctcgag ttcgacccgc tccatcgcga acagcttctg atactgcttg acgagcgcgt tcttcggctc gacgagaatc ttcatcagcg cggcttcatc gagcttgccg agcgtcgcga ccaccggcag acggccgatc agctcgggga tcaacccgaa tttgatcagg tcttccggct cgacctcgcg cagcacttcg cccgcgtccc gctcctgctt gctcttgacc gtcgcgccga aaccgatgcc ggtcttctcg gtgcggtcgg tgatcacctt ctcgaggccg tcgaacgcgc cgccgcaaat gaacaggatg ttggtggtgt cgacctggat gaaatcctgg ttcgggtgct tgcggccgcc ctgcggcggc accgacgcca tcgtgccctc gacgagcttg agcagcgcct gctggacgcc ctcgcccgac acgtcgcggg tgatcgacgg gttgtccgac ttgcggctga tcttgtcgat ttcgtcgatg tagacgatcc cgcgctgcgc cttctcgacc tcgtagttgc agttctgcag cagcttctgg atgatgttct cgacgtcctc gccgacataa ccggnctccg tcagcgtggt cgcgtcggcg atcacgaacg gcacgttcag caggcgcgcg agggtctgcg cgagcagcgt cttgccggag cccgtcgggc cgatcaacag gatgttgctc ttcgacaact cgacatcgtc cttcttgtcg agatgcttcn gngcttgnag tgattgnaga nggnganggc gaggatcttc ntcgcgcgct gnccgatcac gtactgatcg agnangtcnc naatcnnnct gnnggctcca ggtcnnantt ngannngcnn gnttcnnnnn cnnncnnngc nnnncnnnnn nnatnnnnnn nnnnnnnnna nnnntnnnnn nnnnnnnnaa atacnnnngg ncccnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngnnn nnnnnnnnnn nnnngnnnnn nnnnnnnnna nnnccnng
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVPATRPEH LRQKNIAAVS RVFTRSWCRG QTAYYVTHVS GGSTFMADKK GSNSEKLLYC SFCGKSQHEV KKLIAGPSVF ICDECIDLCN EIIRDEAAAA GVEASLSKSD LPSPQEIRDI LDQYVIGQER AKKILAVAVY NHYKRLKHLD KKDDVELSKS NILLIGPTGS GKTLLAQTLA RLLNVPFVIA DATTLTEAGY VGEDVENIIQ KLLQNCNYEV EKAQRGIVYI DEIDKISRKS DNPSITRDVS GEGVQQALLK LVEGTMASVP PQGGRKHPNQ DFIQVDTTNI LFICGGAFDG LEKVITDRTE KTGIGFGATV KSKQERDAGE VLREVEPEDL IKFGLIPELI GRLPVVATLG KLDEAALMKI LVEPKNALVK QYQKLFAMER VELEIRPDAL QAVARKAIRR KTGARGLRSI IEQALLDVMY ELPTLKGVSK VIIDDNVIEG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgcccgcc acacgccccg agcatctgcg gcaaaagaac atcgccgccg tctcccgagt atttaccagg tcatggtgtc ggggacaaac ggcgtattat gtaactcatg tgtccggagg ctctacattc atggcggaca agaaaggttc gaacagcgag aagctgttgt attgctcgtt ttgcggcaaa agccagcatg aggtgaagaa gctcatcgcg gggccgtcgg tattcatctg cgatgaatgt atcgacttgt gcaacgagat catccgcgac gaagcggccg cggcgggcgt cgaagccagc ctgtccaaat ccgacctgcc gagcccgcag gagattcgcg acatcctcga tcagtacgtg atcggccagg agcgcgcgaa gaagatcctc gccgtcgccg tctacaatca ctacaagcgc ctgaagcatc tcgacaagaa ggacgatgtc gagttgtcga agagcaacat cctgttgatc ggcccgacgg gctccggcaa gacgctgctc gcgcagaccc tcgcgcgcct gctgaacgtg ccgttcgtga tcgccgacgc gaccacgctg acggaggccg gttatgtcgg cgaggacgtc gagaacatca tccagaagct gctgcagaac tgcaactacg aggtcgagaa ggcgcagcgc gggatcgtct acatcgacga aatcgacaag atcagccgca agtcggacaa cccgtcgatc acccgcgacg tgtcgggcga gggcgtccag caggcgctgc tcaagctcgt cgagggcacg atggcgtcgg tgccgccgca gggcggccgc aagcacccga accaggattt catccaggtc gacaccacca acatcctgtt catttgcggc ggcgcgttcg acggcctcga gaaggtgatc accgaccgca ccgagaagac cggcatcggt ttcggcgcga cggtcaagag caagcaggag cgggacgcgg gcgaagtgct gcgcgaggtc gagccggaag acctgatcaa attcgggttg atccccgagc tgatcggccg tctgccggtg gtcgcgacgc tcggcaagct cgatgaagcc gcgctgatga agattctcgt cgagccgaag aacgcgctcg tcaagcagta tcagaagctg ttcgcgatgg agcgggtcga actcgagatt cgtccggacg cgctgcaagc cgtcgcccgc aaggcgatcc gccgcaagac gggcgcgcgc gggctgcgtt cgatcatcga gcaggcgctc ctcgacgtga tgtacgaact gccgacgctc aagggcgtca gcaaggtgat catcgacgac aatgtcatcg aaggaaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for BupsA.00030.a.A3.GE27926
HARVESTED ON: 11/24/2009
SEQUENCED ON: 12/3/2009
EXPECTED MW: 52kDa
OBSERVED MW: 50kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 95
Validated AA Sequence
MVPATRPEHL RQKNIAAVSR VFTRSWCRGQ TAYYVTHVSG GSTFMADKKG SNSEKLLYCS FCGKSQHEVK KLIAGPSVFI CDECIDLCNE IIRDEAAAAG VEASLSKSDL PSPQEIRDIL DQYVIGQERA KKILAVAVYN HYKRLKHLDK KDDVELSKSN ILLIGPTGSG KTLLAQTLAR LLNVPFVIAD ATTLTEAGYV GEDVENIIQK LLQNCNYEVE KAQRGIVYID EIDKISRKSD NPSITRDVSG EGVQQALLKL VEGTMASVPP QGGRKHPNQD FIQVDTTNIL FICGGAFDGL EKVITDRTEK TGIGFGATVK SKQERDAGEV LREVEPEDLI KFGLIPELIG RLPVVATLGK LDEAALMKIL VEPKNALVKQ YQKLFAMERV ELEIRPDALQ AVARKAIRRK TGARGLRSII EQALLDVMYE LPTLKGVSKV IIDDNVIEGD GKPLLI
Validated NT Sequence
attagatcaa taacggcttg ccgtctcctt cgatgacatt gtcgtcgatg atcaccttgc tgacgccctt gagcgtcggc agttcgtaca tcacgtcgag gagcgcctgc tcgatgatcg aacgcagccc gcgcgcgccc gtcttgcggc ggatcgcctt gcgggcgacg gcttgcagcg cgtccggacg aatctcgagt tcgacccgct ccatcgcgaa cagcttctga tactgcttga cgagcgcgtt cttcggctcg acgagaatct tcatcagcgc ggcttcatcg agcttgccga gcgtcgcgac caccggcaga cggccgatca gctcggggat caacccgaat ttgatcaggt cttccggctc gacctcgcgc agcacttcgc ccgcgtcccg ctcctgcttg ctcttgaccg tcgcgccgaa accgatgccg gtcttctcgg tgcggtcggt gatcaccttc tcgaggccgt cgaacgcgcc gccgcaaatg aacaggatgt tggtggtgtc gacctggatg aaatcctggt tcgggtgctt gcggccgccc tgcggcggca ccgacgccat cgtgccctcg acgagcttga gcagcgcctg ctggacgccc tcgcccgaca cgtcgcgggt gatcgacggg ttgtccgact tgcggctgat cttgtcgatt tcgtcgatgt agacgatccc gcgctgcgcc ttctcgacct cgtagttgca gttctgcagc agcttctgga tgatgttctc gacgtcctcg ccgacataac cggcctccgt cagcgtggtc gcgtcggcga tcacgaacgg cacgttcagc aggcgcgcga gggtctgcgc gagcagcgtc ttgccggagc ccgtcgggcc gatcaacagg atgttgctct tcgacaactc gacatcgtcc ttcttgtcga gatgcttcag gcgcttgtag tgattgtaga cggcgacggc gaggatcttc ttcgcgcgct cctggccgat cacgtactga tcgaggatgt cgcgaatctc ctgcgggctc ggcaggtcgg atttggacag gctggcttcg acgcccgccg cggccgcttc gtcgcggatg atctcgttgc acaagtcgat acattcatcg cagatgaata ccgacggccc cgcgatgagc ttcttcacct catgctggct tttgccgcaa aacgagcaat acaacagctt ctcgctgttc gaacctttct tgtccgccat gaatgtagag cctccggaca catgagttac ataatacgcc gtttgtcccc gacaccatga cctggtaaat actcgggaga cggcggcgat gttcttttgc cgcagatgct cggggcgtgt ggcgggcacc atcgaaccag gaccctgggt ctgagcttcc agggta
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVPATRPEH LRQKNIAAVS RVFTRSWCRG QTAYYVTHVS GGSTFMADKK GSNSEKLLYC SFCGKSQHEV KKLIAGPSVF ICDECIDLCN EIIRDEAAAA GVEASLSKSD LPSPQEIRDI LDQYVIGQER AKKILAVAVY NHYKRLKHLD KKDDVELSKS NILLIGPTGS GKTLLAQTLA RLLNVPFVIA DATTLTEAGY VGEDVENIIQ KLLQNCNYEV EKAQRGIVYI DEIDKISRKS DNPSITRDVS GEGVQQALLK LVEGTMASVP PQGGRKHPNQ DFIQVDTTNI LFICGGAFDG LEKVITDRTE KTGIGFGATV KSKQERDAGE VLREVEPEDL IKFGLIPELI GRLPVVATLG KLDEAALMKI LVEPKNALVK QYQKLFAMER VELEIRPDAL QAVARKAIRR KTGARGLRSI IEQALLDVMY ELPTLKGVSK VIIDDNVIEG DGKPLLI
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgcccgcc acacgccccg agcatctgcg gcaaaagaac atcgccgccg tctcccgagt atttaccagg tcatggtgtc ggggacaaac ggcgtattat gtaactcatg tgtccggagg ctctacattc atggcggaca agaaaggttc gaacagcgag aagctgttgt attgctcgtt ttgcggcaaa agccagcatg aggtgaagaa gctcatcgcg gggccgtcgg tattcatctg cgatgaatgt atcgacttgt gcaacgagat catccgcgac gaagcggccg cggcgggcgt cgaagccagc ctgtccaaat ccgacctgcc gagcccgcag gagattcgcg acatcctcga tcagtacgtg atcggccagg agcgcgcgaa gaagatcctc gccgtcgccg tctacaatca ctacaagcgc ctgaagcatc tcgacaagaa ggacgatgtc gagttgtcga agagcaacat cctgttgatc ggcccgacgg gctccggcaa gacgctgctc gcgcagaccc tcgcgcgcct gctgaacgtg ccgttcgtga tcgccgacgc gaccacgctg acggaggccg gttatgtcgg cgaggacgtc gagaacatca tccagaagct gctgcagaac tgcaactacg aggtcgagaa ggcgcagcgc gggatcgtct acatcgacga aatcgacaag atcagccgca agtcggacaa cccgtcgatc acccgcgacg tgtcgggcga gggcgtccag caggcgctgc tcaagctcgt cgagggcacg atggcgtcgg tgccgccgca gggcggccgc aagcacccga accaggattt catccaggtc gacaccacca acatcctgtt catttgcggc ggcgcgttcg acggcctcga gaaggtgatc accgaccgca ccgagaagac cggcatcggt ttcggcgcga cggtcaagag caagcaggag cgggacgcgg gcgaagtgct gcgcgaggtc gagccggaag acctgatcaa attcgggttg atccccgagc tgatcggccg tctgccggtg gtcgcgacgc tcggcaagct cgatgaagcc gcgctgatga agattctcgt cgagccgaag aacgcgctcg tcaagcagta tcagaagctg ttcgcgatgg agcgggtcga actcgagatt cgtccggacg cgctgcaagc cgtcgcccgc aaggcgatcc gccgcaagac gggcgcgcgc gggctgcgtt cgatcatcga gcaggcgctc ctcgacgtga tgtacgaact gccgacgctc aagggcgtca gcaaggtgat catcgacgac aatgtcatcg aaggagacgg caagccgtta ttgatcaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for BupsA.00030.a.A4.GE27927
HARVESTED ON: 11/24/2009
SEQUENCED ON: 12/3/2009
EXPECTED MW: 41kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDLPSPQEI RDILDQYVIG QERAKKILAV AVYNHYKRLK HLDKKDDVEL SKSNILLIGP TGSGKTLLAQ TLARLLNVPF VIADATTLTE AGYVGEDVEN IIQKLLQNCN YEVEKAQRGI VYIDEIDKIS RKSDNPSITR DVSGEGVQQA LLKLVEGTMA SVPPQGGRKH PNQDFIQVDT TNILFICGGA FDGLEKVITD RTEKTGIGFG ATVKSKQERD AGEVLREVEP EDLIKFGLIP ELIGRLPVVA TLGKLDEAAL MKILVEPKNA LVKQYQKLFA MERVELEIRP DALQAVARKA IRRKTGARGL RSIIEQALLD VMYELPTLKG VSKVIIDDNV IEGDGKPLLI YEDTPKVAGS N
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggacctgccg agcccgcagg agattcgcga catcctcgat cagtacgtga tcggccagga gcgcgcgaag aagatcctcg ccgtcgccgt ctacaatcac tacaagcgcc tgaagcatct cgacaagaag gacgatgtcg agttgtcgaa gagcaacatc ctgttgatcg gcccgacggg ctccggcaag acgctgctcg cgcagaccct cgcgcgcctg ctgaacgtgc cgttcgtgat cgccgacgcg accacgctga cggaggccgg ttatgtcggc gaggacgtcg agaacatcat ccagaagctg ctgcagaact gcaactacga ggtcgagaag gcgcagcgcg ggatcgtcta catcgacgaa atcgacaaga tcagccgcaa gtcggacaac ccgtcgatca cccgcgacgt gtcgggcgag ggcgtccagc aggcgctgct caagctcgtc gagggcacga tggcgtcggt gccgccgcag ggcggccgca agcacccgaa ccaggatttc atccaggtcg acaccaccaa catcctgttc atttgcggcg gcgcgttcga cggcctcgag aaggtgatca ccgaccgcac cgagaagacc ggcatcggtt tcggcgcgac ggtcaagagc aagcaggagc gggacgcggg cgaagtgctg cgcgaggtcg agccggaaga cctgatcaaa ttcgggttga tccccgagct gatcggccgt ctgccggtgg tcgcgacgct cggcaagctc gatgaagccg cgctgatgaa gattctcgtc gagccgaaga acgcgctcgt caagcagtat cagaagctgt tcgcgatgga gcgggtcgaa ctcgagattc gtccggacgc gctgcaagcc gtcgcccgca aggcgatccg ccgcaagacg ggcgcgcgcg ggctgcgttc gatcatcgag caggcgctcc tcgacgtgat gtacgaactg ccgacgctca agggcgtcag caaggtgatc atcgacgaca atgtcatcga aggagacggc aagccgttat tgatctatga ggacacgccg aaagtggcgg gttcgaataa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for BupsA.00030.a.A5.GE27928
HARVESTED ON: 11/24/2009
SEQUENCED ON: 12/3/2009
EXPECTED MW: 40kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 50
Validated AA Sequence
MASVPPQGGR KHPNQDFIQV DTTNILFICG GAFDGLEKVI TDRTEKTGIG FGATVKSKQE RDAGEVLREV EPEDLIKFGL IPELIGRLPV VATLGKLDEA ALMKILVEPK NALVKQYQKL FAMERVELEI RPDALQAVAR KAIRRKTGAR GLRSIIEQAL LDVMYELPTL KGVSKVIIDD NVIEG
Validated NT Sequence
cnttcgggct ttgttagcag ccggatcctc gagaagcttg gctgcagaac ttgttcgtgc tgtttattat ccttcgatga cattgtcgtc gatgatcacc ttgctgacgc ccttgagcgt cggcagttcg tacatcacgt cgaggagcgc ctgctcgatg atcgaacgca gcccgcgcgc gcccgtcttg cggcggatcg ccttgcgggc gacggcttgc agcgcgtccg gacgaatctc gagttcgacc cgctccatcg cgaacagctt ctgatactgc ttgacgagcg cgttcttcgg ctcgacgaga atcttcatca gcgcggcttc atcgagcttg ccgagcgtcg cgaccaccgg cagacggccg atcagctcgg ggatcaaccc gaatttgatc aggtcttccg gctcgacctc gcgcagcact tcgcccgcgt cccgctcctg cttgctcttg accgtcgcgc cgaaaccgat gccggtcttc tcggtgcggt cggtgatcac cttctcgagg ccgtcgaacg cgccgccgca aatgaacagg atgttggtgg tgtcgacctg gatgaaatcc tggttcgggt gcttgcggcc gccctgcggc ggcaccgacg ccatcgtgcc ctcgacgagc ttgagcagcg cctgctggac gccctcgccc gacacgtcgc gggtgatcga cgggttgtcc gacttgcggc tgatcttgtc gatttcgtcg atgtagacga tcccgcgctg cgccttctcg acctcgtagt tgcagttctg cagcagcttc tggatgatgt tctcgacgtc ctcgccgaca taaccggnct ccgtcagcgt ggtcgcgtcg gcgatcacga acggcacgtt cagcaggcgc gcgagggtct gcgcgagcag cgtcttgccg gagcccgtcg ggccgatcaa caggatgttg ctcttcgaca actcgacatc gtccttcttg tcgagatgct tcaggngctt gtagtgattg nananggnga nggcnangat cttcttcgcg cgctcnggnc gatcacgtac tgatcgagga ngncnnnaan nnnnnnnngg nncggcaggt ccntcnancn gnanccnggg nnnnnancnt ncnnggnnnn nngnnnnnnn nncnngnnnn nnnnncnnnn nnnannnnna nnaaatnnnn tnnnnnnnnn nnnnnnnnnn nnncccnnnn nnnnnncnnn nnnnantnnn nnnnnnnnnn nnncnnnnnn nnnnnnnnnn nnnng
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDLPSPQEI RDILDQYVIG QERAKKILAV AVYNHYKRLK HLDKKDDVEL SKSNILLIGP TGSGKTLLAQ TLARLLNVPF VIADATTLTE AGYVGEDVEN IIQKLLQNCN YEVEKAQRGI VYIDEIDKIS RKSDNPSITR DVSGEGVQQA LLKLVEGTMA SVPPQGGRKH PNQDFIQVDT TNILFICGGA FDGLEKVITD RTEKTGIGFG ATVKSKQERD AGEVLREVEP EDLIKFGLIP ELIGRLPVVA TLGKLDEAAL MKILVEPKNA LVKQYQKLFA MERVELEIRP DALQAVARKA IRRKTGARGL RSIIEQALLD VMYELPTLKG VSKVIIDDNV IEG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggacctgccg agcccgcagg agattcgcga catcctcgat cagtacgtga tcggccagga gcgcgcgaag aagatcctcg ccgtcgccgt ctacaatcac tacaagcgcc tgaagcatct cgacaagaag gacgatgtcg agttgtcgaa gagcaacatc ctgttgatcg gcccgacggg ctccggcaag acgctgctcg cgcagaccct cgcgcgcctg ctgaacgtgc cgttcgtgat cgccgacgcg accacgctga cggaggccgg ttatgtcggc gaggacgtcg agaacatcat ccagaagctg ctgcagaact gcaactacga ggtcgagaag gcgcagcgcg ggatcgtcta catcgacgaa atcgacaaga tcagccgcaa gtcggacaac ccgtcgatca cccgcgacgt gtcgggcgag ggcgtccagc aggcgctgct caagctcgtc gagggcacga tggcgtcggt gccgccgcag ggcggccgca agcacccgaa ccaggatttc atccaggtcg acaccaccaa catcctgttc atttgcggcg gcgcgttcga cggcctcgag aaggtgatca ccgaccgcac cgagaagacc ggcatcggtt tcggcgcgac ggtcaagagc aagcaggagc gggacgcggg cgaagtgctg cgcgaggtcg agccggaaga cctgatcaaa ttcgggttga tccccgagct gatcggccgt ctgccggtgg tcgcgacgct cggcaagctc gatgaagccg cgctgatgaa gattctcgtc gagccgaaga acgcgctcgt caagcagtat cagaagctgt tcgcgatgga gcgggtcgaa ctcgagattc gtccggacgc gctgcaagcc gtcgcccgca aggcgatccg ccgcaagacg ggcgcgcgcg ggctgcgttc gatcatcgag caggcgctcc tcgacgtgat gtacgaactg ccgacgctca agggcgtcag caaggtgatc atcgacgaca atgtcatcga aggaaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for BupsA.00030.a.A6.GE27929
HARVESTED ON: 11/24/2009
SEQUENCED ON: 12/3/2009
EXPECTED MW: 40kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 93
Validated AA Sequence
MDLPSPQEIR DILDQYVIGQ ERAKKILAVA VYNHYKRLKH LDKKDDVELS KSNILLIGPT GSGKTLLAQT LARLLNVPFV IADATTLTEA GYVGEDVENI IQKLLQNCNY EVEKAQRGIV YIDEIDKISR KSDNPSITRD VSGEGVQQAL LKLVEGTMAS VPPQGGRKHP NQDFIQVDTT NILFICGGAF DGLEKVITDR TEKTGIGFGA TVKSKQERDA GEVLREVEPE DLIKFGLIPE LIGRLPVVAT LGKLDEAALM KILVEPKNAL VKQYQKLFAM ERVELEIRPD ALQAVARKAI RRKTGARGLR SIIEQALLDV MYELPTLKGV SKVIIDDNVI EGDGKP
Validated NT Sequence
taccctggaa gctcagaccc agggtcctgg ttcgatggac ctgccgagcc cgcaggagat tcgcgacatc ctcgatcagt acgtgatcgg ccaggagcgc gcgaagaaga tcctcgccgt cgccgtctac aatcactaca agcgcctgaa gcatctcgac aagaaggacg atgtcgagtt gtcgaagagc aacatcctgt tgatcggccc gacgggctcc ggcaagacgc tgctcgcgca gaccctcgcg cgcctgctga acgtgccgtt cgtgatcgcc gacgcgacca cgctgacgga ggccggttat gtcggcgagg acgtcgagaa catcatccag aagctgctgc agaactgcaa ctacgaggtc gagaaggcgc agcgcgggat cgtctacatc gacgaaatcg acaagatcag ccgcaagtcg gacaacccgt cgatcacccg cgacgtgtcg ggcgagggcg tccagcaggc gctgctcaag ctcgtcgagg gcacgatggc gtcggtgccg ccgcagggcg gccgcaagca cccgaaccag gatttcatcc aggtcgacac caccaacatc ctgttcattt gcggcggcgc gttcgacggc ctcgagaagg tgatcaccga ccgcaccgag aagaccggca tcggtttcgg cgcgacggtc aagagcaagc aggagcggga cgcgggcgaa gtgctgcgcg aggtcgagcc ggaagacctg atcaaattcg ggttgatccc cgagctgatc ggccgtctgc cggtggtcgc gacgctcggc aagctcgatg aagccgcgct gatgaagatt ctcgtcgagc cgaagaacgc gctcgtcaag cagtatcaga agctgttcgc gatggagcgg gtcgaactcg agattcgtcc ggacgcgctg caagccgtcg cccgcaaggc gatccgccgc aagacgggcg cgcgcgggct gcgttcgatc atcgagcagg cgctcctcga cgtgatgtac gaactgccga cgctcaaggg cgtcagcaag gtgatcatcg acgacaatgt catcgaagga gacggcaagc cgtta
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDLPSPQEI RDILDQYVIG QERAKKILAV AVYNHYKRLK HLDKKDDVEL SKSNILLIGP TGSGKTLLAQ TLARLLNVPF VIADATTLTE AGYVGEDVEN IIQKLLQNCN YEVEKAQRGI VYIDEIDKIS RKSDNPSITR DVSGEGVQQA LLKLVEGTMA SVPPQGGRKH PNQDFIQVDT TNILFICGGA FDGLEKVITD RTEKTGIGFG ATVKSKQERD AGEVLREVEP EDLIKFGLIP ELIGRLPVVA TLGKLDEAAL MKILVEPKNA LVKQYQKLFA MERVELEIRP DALQAVARKA IRRKTGARGL RSIIEQALLD VMYELPTLKG VSKVIIDDNV IEGDGKPLLI
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggacctgccg agcccgcagg agattcgcga catcctcgat cagtacgtga tcggccagga gcgcgcgaag aagatcctcg ccgtcgccgt ctacaatcac tacaagcgcc tgaagcatct cgacaagaag gacgatgtcg agttgtcgaa gagcaacatc ctgttgatcg gcccgacggg ctccggcaag acgctgctcg cgcagaccct cgcgcgcctg ctgaacgtgc cgttcgtgat cgccgacgcg accacgctga cggaggccgg ttatgtcggc gaggacgtcg agaacatcat ccagaagctg ctgcagaact gcaactacga ggtcgagaag gcgcagcgcg ggatcgtcta catcgacgaa atcgacaaga tcagccgcaa gtcggacaac ccgtcgatca cccgcgacgt gtcgggcgag ggcgtccagc aggcgctgct caagctcgtc gagggcacga tggcgtcggt gccgccgcag ggcggccgca agcacccgaa ccaggatttc atccaggtcg acaccaccaa catcctgttc atttgcggcg gcgcgttcga cggcctcgag aaggtgatca ccgaccgcac cgagaagacc ggcatcggtt tcggcgcgac ggtcaagagc aagcaggagc gggacgcggg cgaagtgctg cgcgaggtcg agccggaaga cctgatcaaa ttcgggttga tccccgagct gatcggccgt ctgccggtgg tcgcgacgct cggcaagctc gatgaagccg cgctgatgaa gattctcgtc gagccgaaga acgcgctcgt caagcagtat cagaagctgt tcgcgatgga gcgggtcgaa ctcgagattc gtccggacgc gctgcaagcc gtcgcccgca aggcgatccg ccgcaagacg ggcgcgcgcg ggctgcgttc gatcatcgag caggcgctcc tcgacgtgat gtacgaactg ccgacgctca agggcgtcag caaggtgatc atcgacgaca atgtcatcga aggagacggc aagccgttat tgatcaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for BupsA.00030.a.A4.PW27874
PURIFICATION DATe: 1/25/2010
CONCENTRATION: 13.44mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 11
VIAL VOLUME: 200µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDLPSPQEI RDILDQYVIG QERAKKILAV AVYNHYKRLK HLDKKDDVEL SKSNILLIGP TGSGKTLLAQ TLARLLNVPF VIADATTLTE AGYVGEDVEN IIQKLLQNCN YEVEKAQRGI VYIDEIDKIS RKSDNPSITR DVSGEGVQQA LLKLVEGTMA SVPPQGGRKH PNQDFIQVDT TNILFICGGA FDGLEKVITD RTEKTGIGFG ATVKSKQERD AGEVLREVEP EDLIKFGLIP ELIGRLPVVA TLGKLDEAAL MKILVEPKNA LVKQYQKLFA MERVELEIRP DALQAVARKA IRRKTGARGL RSIIEQALLD VMYELPTLKG VSKVIIDDNV IEGDGKPLLI YEDTPKVAGS N
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGACCTGCCG AGCCCGCAGG AGATTCGCGA CATCCTCGAT CAGTACGTGA TCGGCCAGGA GCGCGCGAAG AAGATCCTCG CCGTCGCCGT CTACAATCAC TACAAGCGCC TGAAGCATCT CGACAAGAAG GACGATGTCG AGTTGTCGAA GAGCAACATC CTGTTGATCG GCCCGACGGG CTCCGGCAAG ACGCTGCTCG CGCAGACCCT CGCGCGCCTG CTGAACGTGC CGTTCGTGAT CGCCGACGCG ACCACGCTGA CGGAGGCCGG TTATGTCGGC GAGGACGTCG AGAACATCAT CCAGAAGCTG CTGCAGAACT GCAACTACGA GGTCGAGAAG GCGCAGCGCG GGATCGTCTA CATCGACGAA ATCGACAAGA TCAGCCGCAA GTCGGACAAC CCGTCGATCA CCCGCGACGT GTCGGGCGAG GGCGTCCAGC AGGCGCTGCT CAAGCTCGTC GAGGGCACGA TGGCGTCGGT GCCGCCGCAG GGCGGCCGCA AGCACCCGAA CCAGGATTTC ATCCAGGTCG ACACCACCAA CATCCTGTTC ATTTGCGGCG GCGCGTTCGA CGGCCTCGAG AAGGTGATCA CCGACCGCAC CGAGAAGACC GGCATCGGTT TCGGCGCGAC GGTCAAGAGC AAGCAGGAGC GGGACGCGGG CGAAGTGCTG CGCGAGGTCG AGCCGGAAGA CCTGATCAAA TTCGGGTTGA TCCCCGAGCT GATCGGCCGT CTGCCGGTGG TCGCGACGCT CGGCAAGCTC GATGAAGCCG CGCTGATGAA GATTCTCGTC GAGCCGAAGA ACGCGCTCGT CAAGCAGTAT CAGAAGCTGT TCGCGATGGA GCGGGTCGAA CTCGAGATTC GTCCGGACGC GCTGCAAGCC GTCGCCCGCA AGGCGATCCG CCGCAAGACG GGCGCGCGCG GGCTGCGTTC GATCATCGAG CAGGCGCTCC TCGACGTGAT GTACGAACTG CCGACGCTCA AGGGCGTCAG CAAGGTGATC ATCGACGACA ATGTCATCGA AGGAGACGGC AAGCCGTTAT TGATCTATGA GGACACGCCG AAAGTGGCGG GTTCGAATAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA
Details for BupsA.00030.a.A5.PW27875
PURIFICATION DATe: 1/25/2010
CONCENTRATION: 29.69mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 15
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 50
Protocol Notes
notes unavailable
Validated AA Sequence
MASVPPQGGR KHPNQDFIQV DTTNILFICG GAFDGLEKVI TDRTEKTGIG FGATVKSKQE RDAGEVLREV EPEDLIKFGL IPELIGRLPV VATLGKLDEA ALMKILVEPK NALVKQYQKL FAMERVELEI RPDALQAVAR KAIRRKTGAR GLRSIIEQAL LDVMYELPTL KGVSKVIIDD NVIEG
Validated NT Sequence
cnttcgggct ttgttagcag ccggatcctc gagaagcttg gctgcagaac ttgttcgtgc tgtttattat ccttcgatga cattgtcgtc gatgatcacc ttgctgacgc ccttgagcgt cggcagttcg tacatcacgt cgaggagcgc ctgctcgatg atcgaacgca gcccgcgcgc gcccgtcttg cggcggatcg ccttgcgggc gacggcttgc agcgcgtccg gacgaatctc gagttcgacc cgctccatcg cgaacagctt ctgatactgc ttgacgagcg cgttcttcgg ctcgacgaga atcttcatca gcgcggcttc atcgagcttg ccgagcgtcg cgaccaccgg cagacggccg atcagctcgg ggatcaaccc gaatttgatc aggtcttccg gctcgacctc gcgcagcact tcgcccgcgt cccgctcctg cttgctcttg accgtcgcgc cgaaaccgat gccggtcttc tcggtgcggt cggtgatcac cttctcgagg ccgtcgaacg cgccgccgca aatgaacagg atgttggtgg tgtcgacctg gatgaaatcc tggttcgggt gcttgcggcc gccctgcggc ggcaccgacg ccatcgtgcc ctcgacgagc ttgagcagcg cctgctggac gccctcgccc gacacgtcgc gggtgatcga cgggttgtcc gacttgcggc tgatcttgtc gatttcgtcg atgtagacga tcccgcgctg cgccttctcg acctcgtagt tgcagttctg cagcagcttc tggatgatgt tctcgacgtc ctcgccgaca taaccggnct ccgtcagcgt ggtcgcgtcg gcgatcacga acggcacgtt cagcaggcgc gcgagggtct gcgcgagcag cgtcttgccg gagcccgtcg ggccgatcaa caggatgttg ctcttcgaca actcgacatc gtccttcttg tcgagatgct tcaggngctt gtagtgattg nananggnga nggcnangat cttcttcgcg cgctcnggnc gatcacgtac tgatcgagga ngncnnnaan nnnnnnnngg nncggcaggt ccntcnancn gnanccnggg nnnnnancnt ncnnggnnnn nngnnnnnnn nncnngnnnn nnnnncnnnn nnnannnnna nnaaatnnnn tnnnnnnnnn nnnnnnnnnn nnncccnnnn nnnnnncnnn nnnnantnnn nnnnnnnnnn nnncnnnnnn nnnnnnnnnn nnnng
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDLPSPQEI RDILDQYVIG QERAKKILAV AVYNHYKRLK HLDKKDDVEL SKSNILLIGP TGSGKTLLAQ TLARLLNVPF VIADATTLTE AGYVGEDVEN IIQKLLQNCN YEVEKAQRGI VYIDEIDKIS RKSDNPSITR DVSGEGVQQA LLKLVEGTMA SVPPQGGRKH PNQDFIQVDT TNILFICGGA FDGLEKVITD RTEKTGIGFG ATVKSKQERD AGEVLREVEP EDLIKFGLIP ELIGRLPVVA TLGKLDEAAL MKILVEPKNA LVKQYQKLFA MERVELEIRP DALQAVARKA IRRKTGARGL RSIIEQALLD VMYELPTLKG VSKVIIDDNV IEG
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGACCTGCCG AGCCCGCAGG AGATTCGCGA CATCCTCGAT CAGTACGTGA TCGGCCAGGA GCGCGCGAAG AAGATCCTCG CCGTCGCCGT CTACAATCAC TACAAGCGCC TGAAGCATCT CGACAAGAAG GACGATGTCG AGTTGTCGAA GAGCAACATC CTGTTGATCG GCCCGACGGG CTCCGGCAAG ACGCTGCTCG CGCAGACCCT CGCGCGCCTG CTGAACGTGC CGTTCGTGAT CGCCGACGCG ACCACGCTGA CGGAGGCCGG TTATGTCGGC GAGGACGTCG AGAACATCAT CCAGAAGCTG CTGCAGAACT GCAACTACGA GGTCGAGAAG GCGCAGCGCG GGATCGTCTA CATCGACGAA ATCGACAAGA TCAGCCGCAA GTCGGACAAC CCGTCGATCA CCCGCGACGT GTCGGGCGAG GGCGTCCAGC AGGCGCTGCT CAAGCTCGTC GAGGGCACGA TGGCGTCGGT GCCGCCGCAG GGCGGCCGCA AGCACCCGAA CCAGGATTTC ATCCAGGTCG ACACCACCAA CATCCTGTTC ATTTGCGGCG GCGCGTTCGA CGGCCTCGAG AAGGTGATCA CCGACCGCAC CGAGAAGACC GGCATCGGTT TCGGCGCGAC GGTCAAGAGC AAGCAGGAGC GGGACGCGGG CGAAGTGCTG CGCGAGGTCG AGCCGGAAGA CCTGATCAAA TTCGGGTTGA TCCCCGAGCT GATCGGCCGT CTGCCGGTGG TCGCGACGCT CGGCAAGCTC GATGAAGCCG CGCTGATGAA GATTCTCGTC GAGCCGAAGA ACGCGCTCGT CAAGCAGTAT CAGAAGCTGT TCGCGATGGA GCGGGTCGAA CTCGAGATTC GTCCGGACGC GCTGCAAGCC GTCGCCCGCA AGGCGATCCG CCGCAAGACG GGCGCGCGCG GGCTGCGTTC GATCATCGAG CAGGCGCTCC TCGACGTGAT GTACGAACTG CCGACGCTCA AGGGCGTCAG CAAGGTGATC ATCGACGACA ATGTCATCGA AGGAAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA
Details for BupsA.00030.a.A6.PW27876
PURIFICATION DATe: 1/25/2010
CONCENTRATION: 25.7mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 16
VIAL VOLUME: data unavailable
PERCENT IDENTITY: 100
PERCENT COVERAGE: 93
Protocol Notes
notes unavailable
Validated AA Sequence
MDLPSPQEIR DILDQYVIGQ ERAKKILAVA VYNHYKRLKH LDKKDDVELS KSNILLIGPT GSGKTLLAQT LARLLNVPFV IADATTLTEA GYVGEDVENI IQKLLQNCNY EVEKAQRGIV YIDEIDKISR KSDNPSITRD VSGEGVQQAL LKLVEGTMAS VPPQGGRKHP NQDFIQVDTT NILFICGGAF DGLEKVITDR TEKTGIGFGA TVKSKQERDA GEVLREVEPE DLIKFGLIPE LIGRLPVVAT LGKLDEAALM KILVEPKNAL VKQYQKLFAM ERVELEIRPD ALQAVARKAI RRKTGARGLR SIIEQALLDV MYELPTLKGV SKVIIDDNVI EGDGKP
Validated NT Sequence
taccctggaa gctcagaccc agggtcctgg ttcgatggac ctgccgagcc cgcaggagat tcgcgacatc ctcgatcagt acgtgatcgg ccaggagcgc gcgaagaaga tcctcgccgt cgccgtctac aatcactaca agcgcctgaa gcatctcgac aagaaggacg atgtcgagtt gtcgaagagc aacatcctgt tgatcggccc gacgggctcc ggcaagacgc tgctcgcgca gaccctcgcg cgcctgctga acgtgccgtt cgtgatcgcc gacgcgacca cgctgacgga ggccggttat gtcggcgagg acgtcgagaa catcatccag aagctgctgc agaactgcaa ctacgaggtc gagaaggcgc agcgcgggat cgtctacatc gacgaaatcg acaagatcag ccgcaagtcg gacaacccgt cgatcacccg cgacgtgtcg ggcgagggcg tccagcaggc gctgctcaag ctcgtcgagg gcacgatggc gtcggtgccg ccgcagggcg gccgcaagca cccgaaccag gatttcatcc aggtcgacac caccaacatc ctgttcattt gcggcggcgc gttcgacggc ctcgagaagg tgatcaccga ccgcaccgag aagaccggca tcggtttcgg cgcgacggtc aagagcaagc aggagcggga cgcgggcgaa gtgctgcgcg aggtcgagcc ggaagacctg atcaaattcg ggttgatccc cgagctgatc ggccgtctgc cggtggtcgc gacgctcggc aagctcgatg aagccgcgct gatgaagatt ctcgtcgagc cgaagaacgc gctcgtcaag cagtatcaga agctgttcgc gatggagcgg gtcgaactcg agattcgtcc ggacgcgctg caagccgtcg cccgcaaggc gatccgccgc aagacgggcg cgcgcgggct gcgttcgatc atcgagcagg cgctcctcga cgtgatgtac gaactgccga cgctcaaggg cgtcagcaag gtgatcatcg acgacaatgt catcgaagga gacggcaagc cgtta
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDLPSPQEI RDILDQYVIG QERAKKILAV AVYNHYKRLK HLDKKDDVEL SKSNILLIGP TGSGKTLLAQ TLARLLNVPF VIADATTLTE AGYVGEDVEN IIQKLLQNCN YEVEKAQRGI VYIDEIDKIS RKSDNPSITR DVSGEGVQQA LLKLVEGTMA SVPPQGGRKH PNQDFIQVDT TNILFICGGA FDGLEKVITD RTEKTGIGFG ATVKSKQERD AGEVLREVEP EDLIKFGLIP ELIGRLPVVA TLGKLDEAAL MKILVEPKNA LVKQYQKLFA MERVELEIRP DALQAVARKA IRRKTGARGL RSIIEQALLD VMYELPTLKG VSKVIIDDNV IEGDGKPLLI
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGACCTGCCG AGCCCGCAGG AGATTCGCGA CATCCTCGAT CAGTACGTGA TCGGCCAGGA GCGCGCGAAG AAGATCCTCG CCGTCGCCGT CTACAATCAC TACAAGCGCC TGAAGCATCT CGACAAGAAG GACGATGTCG AGTTGTCGAA GAGCAACATC CTGTTGATCG GCCCGACGGG CTCCGGCAAG ACGCTGCTCG CGCAGACCCT CGCGCGCCTG CTGAACGTGC CGTTCGTGAT CGCCGACGCG ACCACGCTGA CGGAGGCCGG TTATGTCGGC GAGGACGTCG AGAACATCAT CCAGAAGCTG CTGCAGAACT GCAACTACGA GGTCGAGAAG GCGCAGCGCG GGATCGTCTA CATCGACGAA ATCGACAAGA TCAGCCGCAA GTCGGACAAC CCGTCGATCA CCCGCGACGT GTCGGGCGAG GGCGTCCAGC AGGCGCTGCT CAAGCTCGTC GAGGGCACGA TGGCGTCGGT GCCGCCGCAG GGCGGCCGCA AGCACCCGAA CCAGGATTTC ATCCAGGTCG ACACCACCAA CATCCTGTTC ATTTGCGGCG GCGCGTTCGA CGGCCTCGAG AAGGTGATCA CCGACCGCAC CGAGAAGACC GGCATCGGTT TCGGCGCGAC GGTCAAGAGC AAGCAGGAGC GGGACGCGGG CGAAGTGCTG CGCGAGGTCG AGCCGGAAGA CCTGATCAAA TTCGGGTTGA TCCCCGAGCT GATCGGCCGT CTGCCGGTGG TCGCGACGCT CGGCAAGCTC GATGAAGCCG CGCTGATGAA GATTCTCGTC GAGCCGAAGA ACGCGCTCGT CAAGCAGTAT CAGAAGCTGT TCGCGATGGA GCGGGTCGAA CTCGAGATTC GTCCGGACGC GCTGCAAGCC GTCGCCCGCA AGGCGATCCG CCGCAAGACG GGCGCGCGCG GGCTGCGTTC GATCATCGAG CAGGCGCTCC TCGACGTGAT GTACGAACTG CCGACGCTCA AGGGCGTCAG CAAGGTGATC ATCGACGACA ATGTCATCGA AGGAGACGGC AAGCCGTTAT TGATCAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA