SSGCID: Target Page

BupsA.00027.a

glutaryl-CoA dehydrogenase

CENTER ID:	BupsA.00027.a
ORGANISM:	Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE:	Melioidosis
CURRENT STATUS:	in PDB
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.00027.a.A1.GE25002	Full length( BupsA.00027.a )		1	395

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.00027.a.A1.PW25040	Full length( BupsA.00027.a )		1	395

PROTEIN: BupsA.00027.a.A1.PW25040

Publications by SSGCID

Probing conformational states of glutaryl-CoA dehydrogenase by fragment screening.

Begley DW, Davies DR, Hartley RC, Hewitt SN, Myler PJ, Rychel AL, Staker BL, Stewart LJ, Van Voorhis WC

Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. - 2010

volume 67, issue Pt 9, pages 1060-9

PMID: 21904051; PMCID: PMC3169403

PDB: 3D6B, 3EOM, 3EON, 3GNC, 3GQT

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_129299
PATRIC ID:	fig\|320372.6.peg.3374
RefSeq:	YP_334609
UniProt:	Q3JP94

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAAATFHWDD PLLLDQQLAD DERMVRDAAH AYAQGKLAPR VTEAFRHETT DAAIFREMGE IGLLGPTIPE QYGGPGLDYV SYGLIAREVE RVDSGYRSMM SVQSSLVMVP IFEFGSDAQK EKYLPKLATG EWIGCFGLTE PNHGSDPGSM VTRARKVPGG YSLSGSKMWI TNSPIADVFV VWAKLDEDGR DEIRGFILEK GCKGLSAPAI HGKVGLRASI TGEIVLDEAF VPEENILPHV KGLRGPFTCL NSARYGIAWG ALGAAESCWH IARQYVLDRK QFGRPLAANQ LIQKKLADMQ TEITLGLQGV LRLGRMKDEG TAAVEITSIM KRNSCGKALD IARLARDMLG GNGISDEFGV ARHLVNLEVV NTYEGTHDIH ALILGRAQTG IQAFF

NT Sequence

ATGGCTGCCG CAACCTTCCA CTGGGACGAT CCGCTGCTGC TCGACCAGCA GCTCGCCGAC GATGAGCGCA TGGTGCGCGA CGCCGCGCAC GCTTACGCGC AAGGCAAGCT CGCGCCGCGC GTGACCGAAG CGTTCCGCCA CGAGACCACC GACGCGGCGA TCTTCCGTGA AATGGGCGAG ATCGGCCTCC TCGGCCCGAC GATCCCCGAG CAGTACGGCG GCCCCGGCCT CGACTACGTG AGTTACGGGC TCATCGCGCG CGAAGTCGAG CGCGTCGATT CGGGTTATCG GTCGATGATG TCGGTGCAAT CGTCGCTCGT GATGGTGCCG ATCTTCGAAT TCGGCTCCGA CGCGCAGAAG GAAAAGTACC TGCCGAAGCT CGCGACGGGC GAATGGATCG GCTGCTTCGG GCTGACCGAG CCGAACCACG GCTCCGATCC CGGCAGCATG GTCACGCGCG CGAGGAAGGT GCCGGGCGGG TACTCGCTGT CCGGCTCGAA GATGTGGATC ACGAATTCGC CGATCGCCGA CGTGTTCGTC GTCTGGGCCA AGCTCGACGA AGACGGCCGC GACGAGATTC GCGGCTTCAT TCTCGAAAAG GGCTGCAAAG GGCTGTCGGC GCCGGCGATC CACGGCAAGG TGGGGCTGCG CGCGTCGATC ACGGGTGAAA TCGTGCTCGA CGAAGCGTTC GTCCCCGAAG AGAACATCCT GCCGCACGTG AAGGGGCTGC GCGGCCCGTT CACGTGCCTG AACTCGGCGC GCTACGGCAT CGCGTGGGGG GCGCTCGGCG CGGCCGAATC GTGCTGGCAC ATCGCGCGCC AATATGTGCT CGATCGCAAG CAGTTCGGCC GCCCGCTCGC CGCGAACCAG TTGATCCAGA AGAAGCTCGC CGACATGCAG ACCGAGATCA CGCTCGGCCT GCAAGGCGTG CTGCGGCTCG GCCGGATGAA GGACGAAGGC ACCGCCGCCG TCGAGATCAC GTCGATCATG AAGCGCAATT CGTGCGGCAA GGCGCTCGAC ATCGCCCGCC TCGCCCGCGA CATGCTGGGC GGCAATGGCA TCTCGGACGA ATTCGGCGTC GCGCGCCACC TCGTGAACCT CGAGGTGGTG AACACGTACG AAGGTACGCA CGACATCCAC GCGCTGATTC TCGGCCGCGC GCAGACGGGG ATTCAGGCGT TCTTC

Details for BupsA.00027.a.A1.GE25002

HARVESTED ON:	2/12/2008
SEQUENCED ON:	6/16/2009
EXPECTED MW:	45kDa
OBSERVED MW:	45kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	99
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMAAATFHWD DPLLLDQQLA DDERMVRDAA HAYAQGKLAP RVTEAFRHET TDAAIFREMG EIGLLGPTIP EQYGGPGLDY VSYGLIAREV ERVDSGYRSM MSVQSSLVMV PIFEFGSDAQ KEKYLPKLAT GEWIGCFGLT EPNHGSDPGS MVTRARKVPG GYSLSGSKMW ITNSPIADVF VVWAKLDEDG RDEIRGFILE KGCKGLSAPA IHGKVGLRAS ITGEIVLDEA FVPEENILPH VKGLRGPFTC LNSARYGIAW GALGAAESCW HIARQYVLDR KQFGRPLAAN QLIQKKLADM QTEITLGLQG VXRLGRXKDE GTAAVEITSI MKRNSCGKAL DIARLARDML GGNGISDEXG VARHLVNLEV VNTYEGTHDI HALILGRAQT

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggctg ccgcaacctt ccactgggac gatccgctgc tgctcgacca gcagctcgcc gacgatgagc gcatggtgcg cgacgccgcg cacgcttacg cgcaaggcaa gctcgcgccg cgcgtgaccg aagcgttccg ccacgagacc accgacgcgg cgatcttccg tgaaatgggc gagatcggcc tcctcggccc gacgatcccc gagcagtacg gcggccctgg cctcgactac gtgagttacg ggctcatcgc gcgcgaagtc gagcgcgtcg attcgggtta tcggtcgatg atgtcggtgc aatcgtcgct cgtgatggtg ccgatcttcg aattcggctc cgacgcgcag aaggaaaagt acctgccgaa gctcgcgacg ggcgaatgga tcggctgctt cgggctgacc gagccgaacc acggctccga tcccggcagc atggtcacgc gcgcgaggaa ggtgccgggc gggtactcgc tgtccggctc gaagatgtgg atcacgaatt cgccgatcgc cgacgtgttc gtcgtctggg ccaagctcga cgaagacggc cgcgacgaga ttcgcggctt cattctcgaa aagggctgca aagggctgtc ggcgccggcg atccacggca aggtggggct gcgcgcgtcg atcacgggtg aaatcgtgct cgacgaagcg ttcgtccccg aagagaacat cctgccgcac gtgaaggggc tgcgcggccc gttcacgtgc ctgaactcgg cgcgctacgg catcgcgtgg ggggcgctcg gcgcggccga atcgtgctgg cacatcgcgc gccaatatgt gctcgatcgc aagcagttcg gccgcccgct cgccgcgaac cagttgatcc agaagaagct cgccgacatg cagaccgaga tcacgctcgg cctgcaaggc gtgntgcggc tcggccggan gaaggacgaa ggcaccgccg ccgtcgagat cacgtcgatc atgaagcgca attcgtgcgg caaggcgctc gacatcgccc gcctcgcccg cgacatgctg ggcggcaatg gcatctcgga cgaattnggc gtcgcgcgcc acctcgtgaa cctcgaggtg gtgaacacgt acgaaggtac gcacgacatc cacgcgctga ttctcggccg cgcgcagacg g

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMAAATFHWD DPLLLDQQLA DDERMVRDAA HAYAQGKLAP RVTEAFRHET TDAAIFREMG EIGLLGPTIP EQYGGPGLDY VSYGLIAREV ERVDSGYRSM MSVQSSLVMV PIFEFGSDAQ KEKYLPKLAT GEWIGCFGLT EPNHGSDPGS MVTRARKVPG GYSLSGSKMW ITNSPIADVF VVWAKLDEDG RDEIRGFILE KGCKGLSAPA IHGKVGLRAS ITGEIVLDEA FVPEENILPH VKGLRGPFTC LNSARYGIAW GALGAAESCW HIARQYVLDR KQFGRPLAAN QLIQKKLADM QTEITLGLQG VLRLGRMKDE GTAAVEITSI MKRNSCGKAL DIARLARDML GGNGISDEFG VARHLVNLEV VNTYEGTHDI HALILGRAQT GIQAFF

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggctgccgca accttccact gggacgatcc gctgctgctc gaccagcagc tcgccgacga tgagcgcatg gtgcgcgacg ccgcgcacgc ttacgcgcaa ggcaagctcg cgccgcgcgt gaccgaagcg ttccgccacg agaccaccga cgcggcgatc ttccgtgaaa tgggcgagat cggcctcctc ggcccgacga tccccgagca gtacggcggc cccggcctcg actacgtgag ttacgggctc atcgcgcgcg aagtcgagcg cgtcgattcg ggttatcggt cgatgatgtc ggtgcaatcg tcgctcgtga tggtgccgat cttcgaattc ggctccgacg cgcagaagga aaagtacctg ccgaagctcg cgacgggcga atggatcggc tgcttcgggc tgaccgagcc gaaccacggc tccgatcccg gcagcatggt cacgcgcgcg aggaaggtgc cgggcgggta ctcgctgtcc ggctcgaaga tgtggatcac gaattcgccg atcgccgacg tgttcgtcgt ctgggccaag ctcgacgaag acggccgcga cgagattcgc ggcttcattc tcgaaaaggg ctgcaaaggg ctgtcggcgc cggcgatcca cggcaaggtg gggctgcgcg cgtcgatcac gggtgaaatc gtgctcgacg aagcgttcgt ccccgaagag aacatcctgc cgcacgtgaa ggggctgcgc ggcccgttca cgtgcctgaa ctcggcgcgc tacggcatcg cgtggggggc gctcggcgcg gccgaatcgt gctggcacat cgcgcgccaa tatgtgctcg atcgcaagca gttcggccgc ccgctcgccg cgaaccagtt gatccagaag aagctcgccg acatgcagac cgagatcacg ctcggcctgc aaggcgtgct gcggctcggc cggatgaagg acgaaggcac cgccgccgtc gagatcacgt cgatcatgaa gcgcaattcg tgcggcaagg cgctcgacat cgcccgcctc gcccgcgaca tgctgggcgg caatggcatc tcggacgaat tcggcgtcgc gcgccacctc gtgaacctcg aggtggtgaa cacgtacgaa ggtacgcacg acatccacgc gctgattctc ggccgcgcgc agacggggat tcaggcgttc ttctgaaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa

Details for BupsA.00027.a.A1.PW25040

PURIFICATION DATe:	3/4/2008
CONCENTRATION:	26.2mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	28
VIAL VOLUME:	100µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	98

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMAAATFHWD DPLLLDQQLA DDERMVRDAA HAYAQGKLAP RVTEAFRHET TDAAIFREMG EIGLLGPTIP EQYGGPGLDY VSYGLIAREV ERVDSGYRSM MSVQSSLVMV PIFEFGSDAQ KEKYLPKLAT GEWIGCFGLT EPNHGSDPGS MVTRARKVPG GYSLSGSKMW ITNSPIADVF VVWAKLDEDG RDEIRGFILE KGCKGLSAPA IHGKVGLRAS ITGEIVLDEA FVPEENILPH VKGLRGPFTC LNSARYGIAW GALGAAESCW HIARQYVLDR KQFGRPLAAN QLIQKKLADM QTEITLGLQG VLRLGRMKDE GTAAVEITSI MKRNSCGKAL DIARLARDML GGNGISDEFG VARHLVNLEV VNTYEGTHDI HALILGRAQT GIQAFF

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCTGCCGCA ACCTTCCACT GGGACGATCC GCTGCTGCTC GACCAGCAGC TCGCCGACGA TGAGCGCATG GTGCGCGACG CCGCGCACGC TTACGCGCAA GGCAAGCTCG CGCCGCGCGT GACCGAAGCG TTCCGCCACG AGACCACCGA CGCGGCGATC TTCCGTGAAA TGGGCGAGAT CGGCCTCCTC GGCCCGACGA TCCCCGAGCA GTACGGCGGC CCCGGCCTCG ACTACGTGAG TTACGGGCTC ATCGCGCGCG AAGTCGAGCG CGTCGATTCG GGTTATCGGT CGATGATGTC GGTGCAATCG TCGCTCGTGA TGGTGCCGAT CTTCGAATTC GGCTCCGACG CGCAGAAGGA AAAGTACCTG CCGAAGCTCG CGACGGGCGA ATGGATCGGC TGCTTCGGGC TGACCGAGCC GAACCACGGC TCCGATCCCG GCAGCATGGT CACGCGCGCG AGGAAGGTGC CGGGCGGGTA CTCGCTGTCC GGCTCGAAGA TGTGGATCAC GAATTCGCCG ATCGCCGACG TGTTCGTCGT CTGGGCCAAG CTCGACGAAG ACGGCCGCGA CGAGATTCGC GGCTTCATTC TCGAAAAGGG CTGCAAAGGG CTGTCGGCGC CGGCGATCCA CGGCAAGGTG GGGCTGCGCG CGTCGATCAC GGGTGAAATC GTGCTCGACG AAGCGTTCGT CCCCGAAGAG AACATCCTGC CGCACGTGAA GGGGCTGCGC GGCCCGTTCA CGTGCCTGAA CTCGGCGCGC TACGGCATCG CGTGGGGGGC GCTCGGCGCG GCCGAATCGT GCTGGCACAT CGCGCGCCAA TATGTGCTCG ATCGCAAGCA GTTCGGCCGC CCGCTCGCCG CGAACCAGTT GATCCAGAAG AAGCTCGCCG ACATGCAGAC CGAGATCACG CTCGGCCTGC AAGGCGTGCT GCGGCTCGGC CGGATGAAGG ACGAAGGCAC CGCCGCCGTC GAGATCACGT CGATCATGAA GCGCAATTCG TGCGGCAAGG CGCTCGACAT CGCCCGCCTC GCCCGCGACA TGCTGGGCGG CAATGGCATC TCGGACGAAT TCGGCGTCGC GCGCCACCTC GTGAACCTCG AGGTGGTGAA CACGTACGAA GGTACGCACG ACATCCACGC GCTGATTCTC GGCCGCGCGC AGACGGGGAT TCAGGCGTTC TTCTGAAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA