BupsA.00023.a
inorganic pyrophosphatase
| CENTER ID: | BupsA.00023.a |
| ORGANISM: | Burkholderia pseudomallei 1710b |
| ASSOCIATED DISEASE: | Melioidosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BupsA.00023.a.A1.GE24998 | Full length( BupsA.00023.a ) | 1 | 175 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
3D63
DEPOSITED: 5/18/2008
DETERMINATION: XRay
CLONE: BupsA.00023.a.A1.GE24998
PROTEIN: BupsA.00023.a.A1.PW25498
3EIY
DEPOSITED: 9/17/2008
DETERMINATION: XRay
CLONE: BupsA.00023.a.A1.GE24998
PROTEIN: BupsA.00023.a.A1.PW25498
3EIZ
DEPOSITED: 9/17/2008
DETERMINATION: XRay
CLONE: BupsA.00023.a.A1.GE24998
PROTEIN: BupsA.00023.a.A1.PW25498
3EJ0
DEPOSITED: 9/17/2008
DETERMINATION: XRay
CLONE: BupsA.00023.a.A1.GE24998
PROTEIN: BupsA.00023.a.A1.PW25498
3EJ2
DEPOSITED: 9/17/2008
DETERMINATION: XRay
CLONE: BupsA.00023.a.A1.GE24998
PROTEIN: BupsA.00023.a.A1.PW25498
3GVF
DEPOSITED: 3/30/2009
DETERMINATION: XRay
CLONE: BupsA.00023.a.A1.GE24998
PROTEIN: BupsA.00023.a.A1.PW25498
Publications by SSGCID
The role of medical structural genomics in discovering new drugs for infectious diseases.
Hol WG, Myler PJ, Stewart LJ, Van Voorhis WC
PLoS Comput. Biol. - 2009
volume 5, issue 10, pages e1000530
PMID: 19855826; PMCID: PMC2756625
Combining functional and structural genomics to sample the essential Burkholderia structome.
Abendroth J, Armour B, Barrett L, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Fox D, Gallagher LA, Gardberg AS, Gillespie A, Manoil C, Myler PJ, Nakazawa-Hewitt S, Napuli A, Nguyen MT, Patrapuvich R, Phan I, Stacy R, Staker BL, Stewart LJ, Van Voorhis WC
PLoS ONE - 2012
volume 8, issue 1, pages e53851
PMID: 23382856; PMCID: PMC3561365
PDB:
3D63,
3DAH,
3EIZ,
3EJ2,
3EK2,
3EZO,
3F0F,
3FTP,
3GK0,
3GK3,
3GVF,
3GWA,
3GWE,
3IML,
3SZ8,
3T4C,
3TML,
3TMQ,
3TXY,
3U7J,
3UE9,
3UK1,
3UK2,
3UND,
3UPT,
3URR,
3UW1,
3UW2,
3UW3,
3V2I,
3V7N,
3V8H,
3V9O,
3V9P,
3VAV,
4DDO,
4DFE,
4DHE,
4DHK,
4DUT,
4DZ4,
4E4T,
4E51,
4EFI,
4EG0,
4EGJ,
4EK2,
4EQY,
4EWG,
4EX5,
4EXQ,
4F2G,
4F32,
4F3N,
4F3Y,
4F4H,
4F7D,
4FK8,
4FRY,
4G1K,
4G67,
4G6Z,
4GHK,
4H3Y,
4H3Z,
4H4G
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_128875 |
| PATRIC ID: | fig|320372.6.peg.1247 |
| RefSeq: | YP_332648 |
| UniProt: | Q3JUV5 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSFSNVPAGK DLPQDFNVII EIPAQSEPVK YEADKALGLL VVDRFIGTGM RYPVNYGFIP QTLSGDGDPV DVLVITPFPL LAGSVVRARA LGMLKMTDES GVDAKLVAVP HDKVCPMTAN LKSIDDVPAY LKDQIKHFFE QYKALEKGKW VKVEGWDGID AAHKEITDGV ANFKK
NT Sequence
ATGAGCTTCA GCAATGTTCC GGCCGGCAAG GATCTGCCGC AAGACTTCAA CGTGATCATC GAGATCCCGG CGCAAAGCGA GCCGGTCAAG TACGAAGCCG ACAAGGCGCT CGGCCTCCTC GTCGTCGACC GCTTCATCGG CACCGGCATG CGCTACCCGG TGAACTACGG CTTCATCCCG CAGACGCTGT CGGGCGACGG CGACCCCGTC GACGTACTCG TCATCACGCC GTTTCCGCTG CTCGCGGGCT CGGTCGTGCG CGCGCGCGCG CTCGGCATGC TGAAGATGAC CGACGAATCG GGCGTCGACG CGAAGCTCGT CGCGGTGCCG CACGACAAGG TCTGCCCGAT GACGGCCAAC CTCAAGTCGA TCGACGACGT GCCCGCGTAC CTGAAGGATC AGATCAAGCA CTTCTTCGAG CAATACAAGG CGCTCGAGAA GGGCAAGTGG GTGAAGGTCG AGGGCTGGGA CGGCATCGAC GCCGCGCACA AGGAAATCAC CGACGGCGTC GCGAACTTCA AGAAG
Details for BupsA.00023.a.A1.GE24998
| HARVESTED ON: | 2/12/2008 |
| SEQUENCED ON: | 6/16/2009 |
| EXPECTED MW: | 21kDa |
| OBSERVED MW: | 22kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 98 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSFSNVPAG KDLPQDFNVI IEIPAQSEPV KYEADKALGL LVVDRFIGTG MRYPVNYGFI PQTLSGDGDP VDVLVITPFP LLAGSVVRAR ALGMLKMTDE SGVDAKLVAV PHDKVCPMTA NLKSIDDVPA YLKDQIKHFF EQYKALEKGK WVKVEGWDGI DAAHKEITDG VANF
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgagct tcagcaatgt tccggccggc aaggatctgc cgcaagactt caacgtgatc atcgagatcc cggcgcaaag cgagccggtc aagtacgaag ccgacaaggc gctcggcctc ctcgtcgtcg accgcttcat cggcaccggc atgcgctacc cggtgaacta cggcttcatc ccgcagacgc tgtcgggcga cggcgacccc gtcgacgtac tcgtcatcac gccgtttccg ctgctcgcgg gctcggtcgt gcgcgcgcgc gcgctcggca tgctgaagat gaccgacgaa tcgggcgtcg acgcgaagct cgtcgcggtg ccgcacgaca aggtctgccc gatgacggcc aacctcaagt cgatcgacga cgtgcccgcg tacctgaagg atcagatcaa gcacttcttc gagcaataca aggcgctcga gaagggcaag tgggtgaagg tcgagggctg ggacggcatc gacgccgcgc acaaggaaat caccgacggc gtcgcgaact tca
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSFSNVPAG KDLPQDFNVI IEIPAQSEPV KYEADKALGL LVVDRFIGTG MRYPVNYGFI PQTLSGDGDP VDVLVITPFP LLAGSVVRAR ALGMLKMTDE SGVDAKLVAV PHDKVCPMTA NLKSIDDVPA YLKDQIKHFF EQYKALEKGK WVKVEGWDGI DAAHKEITDG VANFKK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcttcagc aatgttccgg ccggcaagga tctgccgcaa gacttcaacg tgatcatcga gatcccggcg caaagcgagc cggtcaagta cgaagccgac aaggcgctcg gcctcctcgt cgtcgaccgc ttcatcggca ccggcatgcg ctacccggtg aactacggct tcatcccgca gacgctgtcg ggcgacggcg accccgtcga cgtactcgtc atcacgccgt ttccgctgct cgcgggctcg gtcgtgcgcg cgcgcgcgct cggcatgctg aagatgaccg acgaatcggg cgtcgacgcg aagctcgtcg cggtgccgca cgacaaggtc tgcccgatga cggccaacct caagtcgatc gacgacgtgc ccgcgtacct gaaggatcag atcaagcact tcttcgagca atacaaggcg ctcgagaagg gcaagtgggt gaaggtcgag ggctgggacg gcatcgacgc cgcgcacaag gaaatcaccg acggcgtcgc gaacttcaag aagtaaaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa