BupsA.00020.b
betaine aldehyde dehydrogenase
| CENTER ID: | BupsA.00020.b |
| ORGANISM: | Burkholderia pseudomallei 1710b |
| ASSOCIATED DISEASE: | Melioidosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BupsA.00020.b.A2.GE27915 | Created from Decode construct for feature BupsA.00020.b | 1 | 480 | ||
| BupsA.00020.b.A3.GE27916 | Created from Decode construct for feature BupsA.00020.b | 5 | 489 | ||
| BupsA.00020.b.A4.GE27917 | Created from Decode construct for feature BupsA.00020.b | 5 | 480 | ||
| BupsA.00020.b.A5.GE27918 | Created from Decode construct for feature BupsA.00020.b | 12 | 489 | ||
| BupsA.00020.b.A6.GE27919 | Created from Decode construct for feature BupsA.00020.b | 12 | 480 | ||
| BupsA.00020.b.AE1.GE43326 | Full length( BupsA.00020.b ) | 1 | 489 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BupsA.00020.b.B1.PS00017 | Full length( BupsA.00020.b ) | 1 | 489 | ||
| BupsA.00020.b.AE1.PS38619 | Full length( BupsA.00020.b ) | 1 | 489 | ||
| BupsA.00020.b.A3.PW27863 | Created from Decode construct for feature BupsA.00020.b | 5 | 489 | ||
| BupsA.00020.b.A2.PW28611 | Created from Decode construct for feature BupsA.00020.b | 1 | 480 |
Structures
6WSA
DEPOSITED: 4/30/2020
DETERMINATION: XRay
CLONE: BupsA.00020.b.AE1.GE43326
PROTEIN: BupsA.00020.b.AE1.PS38619
6WSB
DEPOSITED: 4/30/2020
DETERMINATION: XRay
CLONE: BupsA.00020.b.AE1.GE43326
PROTEIN: BupsA.00020.b.AE1.PS38619
Publications by SSGCID
Crystal structure of betaine aldehyde dehydrogenase from Burkholderia pseudomallei
Abendroth JA, Edwards TE, Myler PJ, Subramanian S, Asojo OA, Beard D
Acta Crystallogr F Struct Biol Commun - 2022
volume 78, issue Pt 2, pages 45-51
PMID: 35102892; PMCID: PMC8805214
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_131437 |
| PATRIC ID: | fig|320372.6.peg.4371 |
| RefSeq: | YP_335535 |
| UniProt: | Q3JLL8 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSVYGLQRLY IAGAHADATS GKTFDTFDPA TGELLARVQQ ASADDVDRAV ASAREGQREW AAMTAMQRSR ILRRAVELLR ERNDALAELE MRDTGKPIAE TRAVDIVTGA DVIEYYAGLA TAIEGLQVPL RPESFVYTRR EPLGVCAGIG AWNYPIQIAC WKSAPALAAG NAMIFKPSEV TPLSALKLAE IYTEAGVPAG VFNVVQGDGS VGALLSAHPG IAKVSFTGGV ETGKKVMSLA GASSLKEVTM ELGGKSPLIV FDDADLDRAA DIAVTANFFS AGQVCTNGTR VFVQQAVKDA FVERVLARVA RIRVGKPSDS DTNFGPLASA AQLDKVLGYI DSGKAEGAKL LAGGARLVND HFASGQYVAP TVFGDCRDDM RIVREEIFGP VMSILSFETE DEAIARANAT DYGLAAGVVT ENLSRAHRAI HRLEAGICWI NTWGESPAEM PVGGYKQSGV GRENGITTLE HYTRIKSVQV ELGRYQPVF
NT Sequence
ATGTCCGTAT ACGGTCTGCA GCGCCTTTAC ATCGCCGGCG CCCACGCCGA CGCCACGAGC GGCAAGACGT TCGATACGTT CGATCCCGCG ACGGGCGAAT TGCTCGCGCG CGTTCAGCAG GCGAGCGCCG ACGATGTCGA CCGCGCGGTC GCATCCGCGC GCGAAGGCCA GCGCGAATGG GCCGCGATGA CCGCGATGCA GCGCTCGCGC ATCCTGCGTC GCGCGGTCGA ATTGCTGCGC GAGCGCAACG ACGCGCTCGC CGAGCTCGAG ATGCGCGACA CGGGCAAGCC GATCGCGGAA ACGCGCGCCG TCGACATCGT CACGGGCGCC GACGTGATCG AGTACTACGC GGGCCTCGCG ACCGCGATCG AAGGTCTGCA GGTGCCGCTG CGCCCGGAAT CGTTCGTCTA CACGCGGCGC GAGCCGCTCG GCGTGTGCGC GGGCATCGGC GCATGGAACT ACCCGATCCA GATCGCCTGC TGGAAGAGCG CGCCCGCGCT CGCCGCCGGC AACGCGATGA TCTTCAAGCC GAGCGAAGTC ACGCCGCTGT CCGCGCTCAA GCTCGCGGAG ATCTACACCG AGGCGGGAGT GCCCGCCGGC GTGTTCAACG TCGTGCAGGG CGACGGCTCG GTCGGCGCGC TGCTCAGCGC GCACCCGGGC ATCGCGAAGG TATCGTTTAC GGGCGGCGTC GAAACCGGCA AGAAGGTGAT GTCGCTCGCG GGCGCATCGT CGCTGAAGGA AGTGACGATG GAACTGGGCG GCAAATCGCC GCTCATCGTG TTCGACGACG CCGATCTCGA CCGCGCGGCC GACATCGCCG TCACCGCGAA CTTCTTCAGC GCGGGCCAGG TGTGCACGAA CGGCACGCGC GTGTTCGTCC AGCAAGCGGT GAAGGACGCG TTCGTCGAGC GCGTGCTCGC GCGCGTCGCA CGGATTCGGG TCGGCAAACC TTCGGATTCC GACACGAATT TCGGACCGCT CGCGAGCGCC GCCCAGCTCG ACAAGGTGCT CGGCTACATC GACAGCGGCA AGGCCGAAGG CGCGAAGCTG CTCGCGGGCG GCGCGCGCCT CGTGAACGAT CACTTCGCGA GCGGCCAGTA CGTTGCGCCG ACCGTGTTCG GCGATTGCCG CGACGACATG AGGATCGTCC GCGAAGAGAT TTTCGGGCCG GTGATGAGCA TCCTGTCGTT CGAAACGGAA GACGAAGCGA TCGCGCGCGC GAACGCGACC GACTACGGCC TCGCGGCGGG CGTCGTCACC GAGAACCTGT CGCGCGCGCA CCGCGCGATC CATCGCCTCG AAGCCGGCAT CTGCTGGATC AACACGTGGG GCGAATCGCC CGCCGAGATG CCGGTTGGCG GGTATAAGCA ATCCGGCGTC GGACGCGAGA ACGGCATCAC GACGCTCGAG CACTACACTC GAATCAAATC GGTGCAGGTC GAGCTCGGCC GCTATCAACC GGTGTTC
Details for BupsA.00020.b.A2.GE27915
| HARVESTED ON: | 11/24/2009 |
| SEQUENCED ON: | 12/3/2009 |
| EXPECTED MW: | 53kDa |
| OBSERVED MW: | 50kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Few (1-10) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 98 |
| PERCENT COVERAGE: | 63 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSVYGLQRL YIAGAHADAT SGKTFDTFDP ATGELLARVQ QASADDVDRA VASAREGQRE WAAMTAMQRS RILRRAVELL RERNDALAEL EMRDTGKPIA ETRAVDIVTG ADVIEYYAGL ATAIEGLQVP LRPESFVYTR REPLGVCAGI GAWNYPIQIA CWKSAPALAA GNAMIFKPSE VTPLSALKLA EIYTEAGVPA GVFNVVQGDG SVGALLSAHP GIAXVSFTGG VETGKKVMSL AGASSLKEVT MELGGKSPLI VFDDAXLDRA ADIAVTANFF SAGXXCTNGT RVFVQQ
Validated NT Sequence
tttaagaagg aganatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgtccgt atacggtctg cagcgccttt acatcgccgg cgcccacgcc gacgccacga gcggcaagac gttcgatacg ttcgatcccg cgacgggcga attgctcgcg cgcgttcagc aggcgagcgc cgacgatgtc gaccgcgcgg tcgcatccgc gcgcgaaggc cagcgcgaat gggccgcgat gaccgcgatg cagcgctcgc gcatcctgcg tcgcgcggtc gaattgctgc gcgagcgcaa cgacgcgctc gccgagctcg agatgcgcga cacgggcaag ccgatcgcgg aaacgcgcgc cgtcgacatc gtcacgggcg ccgacgtgat cgagtactac gcgggcctcg cgaccgcgat cgaaggtctg caggtgccgc tgcgcccgga atcgttcgtc tacacgcggc gcgagccgct cggcgtgtgc gcgggcatcg gcgcatggaa ctacccgatc cagatcgcct gctggaagag cgcgcccgcg ctcgccgccg gcaacgcgat gatcttcaag ccgagcgaag tcacgccgct gtccgcgctc aagctcgcgg agatctacac cgaggcggga gtgcccgccg gcgtgttcaa cgtcgtgcag ggcgacggct cggtcggcgc gctgctcagc gcgcacccgg gcatcgcgaa ngtatcgttt acgggcggcg tcgaaaccgg caagaaggtg atgtcgctcg cgggcgcatc gtcgctgaag gaagtgacga tggaactggg cggcaaatcg ccgctcatcg tgttcgacga cgccnatctc gaccgcgcgg ccgacatcgc cgtcaccgcg aacttcttca gcgcgggcca ngngtgcacg aacggcacgc gcgtgttcgt ccagcaagcg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSVYGLQRL YIAGAHADAT SGKTFDTFDP ATGELLARVQ QASADDVDRA VASAREGQRE WAAMTAMQRS RILRRAVELL RERNDALAEL EMRDTGKPIA ETRAVDIVTG ADVIEYYAGL ATAIEGLQVP LRPESFVYTR REPLGVCAGI GAWNYPIQIA CWKSAPALAA GNAMIFKPSE VTPLSALKLA EIYTEAGVPA GVFNVVQGDG SVGALLSAHP GIAKVSFTGG VETGKKVMSL AGASSLKEVT MELGGKSPLI VFDDADLDRA ADIAVTANFF SAGQVCTNGT RVFVQQAVKD AFVERVLARV ARIRVGKPSD SDTNFGPLAS AAQLDKVLGY IDSGKAEGAK LLAGGARLVN DHFASGQYVA PTVFGDCRDD MRIVREEIFG PVMSILSFET EDEAIARANA TDYGLAAGVV TENLSRAHRA IHRLEAGICW INTWGESPAE MPVGGYKQSG VGRENGITTL EHYTRIKSVQ V
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtccgtatac ggtctgcagc gcctttacat cgccggcgcc cacgccgacg ccacgagcgg caagacgttc gatacgttcg atcccgcgac gggcgaattg ctcgcgcgcg ttcagcaggc gagcgccgac gatgtcgacc gcgcggtcgc atccgcgcgc gaaggccagc gcgaatgggc cgcgatgacc gcgatgcagc gctcgcgcat cctgcgtcgc gcggtcgaat tgctgcgcga gcgcaacgac gcgctcgccg agctcgagat gcgcgacacg ggcaagccga tcgcggaaac gcgcgccgtc gacatcgtca cgggcgccga cgtgatcgag tactacgcgg gcctcgcgac cgcgatcgaa ggtctgcagg tgccgctgcg cccggaatcg ttcgtctaca cgcggcgcga gccgctcggc gtgtgcgcgg gcatcggcgc atggaactac ccgatccaga tcgcctgctg gaagagcgcg cccgcgctcg ccgccggcaa cgcgatgatc ttcaagccga gcgaagtcac gccgctgtcc gcgctcaagc tcgcggagat ctacaccgag gcgggagtgc ccgccggcgt gttcaacgtc gtgcagggcg acggctcggt cggcgcgctg ctcagcgcgc acccgggcat cgcgaaggta tcgtttacgg gcggcgtcga aaccggcaag aaggtgatgt cgctcgcggg cgcatcgtcg ctgaaggaag tgacgatgga actgggcggc aaatcgccgc tcatcgtgtt cgacgacgcc gatctcgacc gcgcggccga catcgccgtc accgcgaact tcttcagcgc gggccaggtg tgcacgaacg gcacgcgcgt gttcgtccag caagcggtga aggacgcgtt cgtcgagcgc gtgctcgcgc gcgtcgcacg gattcgggtc ggcaaacctt cggattccga cacgaatttc ggaccgctcg cgagcgccgc ccagctcgac aaggtgctcg gctacatcga cagcggcaag gccgaaggcg cgaagctgct cgcgggcggc gcgcgcctcg tgaacgatca cttcgcgagc ggccagtacg ttgcgccgac cgtgttcggc gattgccgcg acgacatgag gatcgtccgc gaagagattt tcgggccggt gatgagcatc ctgtcgttcg aaacggaaga cgaagcgatc gcgcgcgcga acgcgaccga ctacggcctc gcggcgggcg tcgtcaccga gaacctgtcg cgcgcgcacc gcgcgatcca tcgcctcgaa gccggcatct gctggatcaa cacgtggggc gaatcgcccg ccgagatgcc ggttggcggg tataagcaat ccggcgtcgg acgcgagaac ggcatcacga cgctcgagca ctacactcga atcaaatcgg tgcaggtcaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for BupsA.00020.b.A3.GE27916
| HARVESTED ON: | 11/24/2009 |
| SEQUENCED ON: | 12/3/2009 |
| EXPECTED MW: | 54kDa |
| OBSERVED MW: | 50kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Few (1-10) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGLQRLYIA GAHADATSGK TFDTFDPATG ELLARVQQAS ADDVDRAVAS AREGQREWAA MTAMQRSRIL RRAVELLRER NDALAELEMR DTGKPIAETR AVDIVTGADV IEYYAGLATA IEGLQVPLRP ESFVYTRREP LGVCAGIGAW NYPIQIACWK SAPALAAGNA MIFKPSEVTP LSALKLAEIY TEAGVPAGVF NVVQGDGSVG ALLSAHPGIA KVSFTGGVET GKKVMSLAGA SSLKEVTMEL GGKSPLIVFD DADLDRAADI AVTANFFSAG QVCTNGTRVF VQQAVKDAFV ERVLARVARI RVGKPSDSDT NFGPLASAAQ LDKVLGYIDS GKAEGAKLLA GGARLVNDHF ASGQYVAPTV FGDCRDDMRI VREEIFGPVM SILSFETEDE AIARANATDY GLAAGVVTEN LSRAHRAIHR LEAGICWINT WGESPAEMPV GGYKQSGVGR ENGITTLEHY TRIKSVQVEL GRYQPVF
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gggtctgcag cgcctttaca tcgccggcgc ccacgccgac gccacgagcg gcaagacgtt cgatacgttc gatcccgcga cgggcgaatt gctcgcgcgc gttcagcagg cgagcgccga cgatgtcgac cgcgcggtcg catccgcgcg cgaaggccag cgcgaatggg ccgcgatgac cgcgatgcag cgctcgcgca tcctgcgtcg cgcggtcgaa ttgctgcgcg agcgcaacga cgcgctcgcc gagctcgaga tgcgcgacac gggcaagccg atcgcggaaa cgcgcgccgt cgacatcgtc acgggcgccg acgtgatcga gtactacgcg ggcctcgcga ccgcgatcga aggtctgcag gtgccgctgc gcccggaatc gttcgtctac acgcggcgcg agccgctcgg cgtgtgcgcg ggcatcggcg catggaacta cccgatccag atcgcctgct ggaagagcgc gcccgcgctc gccgccggca acgcgatgat cttcaagccg agcgaagtca cgccgctgtc cgcgctcaag ctcgcggaga tctacaccga ggcgggagtg cccgccggcg tgttcaacgt cgtgcagggc gacggctcgg tcggcgcgct gctcagcgcg cacccgggca tcgcgaaggt atcgtttacg ggcggcgtcg aaaccggcaa gaaggtgatg tcgctcgcgg gcgcatcgtc gctgaaggaa gtgacgatgg aactgggcgg caaatcgccg ctcatcgtgt tcgacgacgc cgatctcgac cgcgcggccg acatcgccgt caccgcgaac ttcttcagcg cgggccaggt gtgcacgaac ggcacgcgcg tgttcgtcca gcaagcggtg aaggacgcgt tcgtcgagcg cgtgctcgcg cgcgtcgcac ggattcgggt cggcaaacct tcggattccg acacgaattt cggaccgctc gcgagcgccg cccagctcga caaggtgctc ggctacatcg acagcggcaa ggccgaaggc gcgaagctgc tcgcgggcgg cgcgcgcctc gtgaacgatc acttcgcgag cggccagtac gttgcgccga ccgtgttcgg cgattgccgc gacgacatga ggatcgtccg cgaagagatt ttcgggccgg tgatgagcat cctgtcgttc gaaacggaag acgaagcgat cgcgcgcgcg aacgcgaccg actacggcct cgcggcgggc gtcgtcaccg agaacctgtc gcgcgcgcac cgcgcgatcc atcgcctcga agccggcatc tgctggatca acacgtgggg cgaatcgccc gccgagatgc cggttggcgg gtataagcaa tccggcgtcg gacgcgagaa cggcatcacg acgctcgagc actacactcg aatcaaatcg gtgcaggtcg agctcggccg ctatcaaccg gtgttcaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for BupsA.00020.b.A4.GE27917
| HARVESTED ON: | 11/24/2009 |
| SEQUENCED ON: | 12/3/2009 |
| EXPECTED MW: | 53kDa |
| OBSERVED MW: | 50kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | No Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 79 |
| PERCENT COVERAGE: | 72 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMGLQRLYIA GAHADATSGK TFDTFDPATG ELLARVQQAS ADDVDRAVAS AREGQREWAA MTAMQRSRIL RRAVELLRER NDALAELEMR DTGKPIAETR AVDIVTGADV IEYYAGLATA IEGLQVPLRP ESFVYTRREP LGVCAGIGAW NYPIQIACWK SAPALAAGNA MIFKPSEVTP LSALKLAEIY TEAGVPAGVF NVVQGDGSVX ALLSAHPGIA KVSXTGGVXT GKKVMSLAGA SSLKEVTMEL GGXSPLIVXX DAXLDRAXDX AVPXNFFXAX XXXXTARXXS XXXXXXXXXX XXVXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XX
Validated NT Sequence
tannnnnnnt nnnnnnnttt aagaaggaga nataccatgg ctcatcacca tcaccatcat atgggtaccc tggaagctca gacccagggt cctggttcga tgggtctgca gcgcctttac atcgccggcg cccacgccga cgccacgagc ggcaagacgt tcgatacgtt cgatcccgcg acgggcgaat tgctcgcgcg cgttcagcag gcgagcgccg acgatgtcga ccgcgcggtc gcatccgcgc gcgaaggcca gcgcgaatgg gccgcgatga ccgcgatgca gcgctcgcgc atcctgcgtc gcgcggtcga attgctgcgc gagcgcaacg acgcgctcgc cgagctcgag atgcgcgaca cgggcaagcc gatcgcggaa acgcgcgccg tcgacatcgt cacgggcgcc gacgtgatcg agtactacgc gggcctcgcg accgcgatcg aaggtctgca ggtgccgctg cgcccggaat cgttcgtcta cacgcggcgc gagccgctcg gcgtgtgcgc gggcatcggc gcatggaact acccgatcca gatcgcctgc tggaagagcg cgcccgcgct cgccgccggc aacgcgatga tcttcaagcc gagcgaagtc acgccgctgt ccgcgctcaa gctcgcggag atctacaccg aggcgggagt gcccgccggc gtgttcaacg tcgtgcaggg cgacggctcg gtcgncgcgc tgctcagcgc gcacccgggc atcgcgaagg tatcgnttac gggcggcgtc naaaccggca agaaggtgat gtcgctcgcg ggcgcatcgt cgctgaagga agtgacgatg gaactgggcg gcaantcgcc gctcatcgtg tncgangacg ccnatctcga ccgcgcggnc gacntcgccg tccccgngaa cttcttcngc gcnnncagnn gtgnnnnnac ggcacgnncg tgntcnnnnc nnnnannnnn naangnnngt tncnncnnnn nngtgnnnnc ncnnntnnnn nnnnntcngn nngnannnnc nnnntnncnn nnnnannntn nnnnnnnnnn nnnnnnnnnn nntnnnnnnn nnnnnnnnnn ntnnnnnnnn nnnnngnnan nnnng
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGLQRLYIA GAHADATSGK TFDTFDPATG ELLARVQQAS ADDVDRAVAS AREGQREWAA MTAMQRSRIL RRAVELLRER NDALAELEMR DTGKPIAETR AVDIVTGADV IEYYAGLATA IEGLQVPLRP ESFVYTRREP LGVCAGIGAW NYPIQIACWK SAPALAAGNA MIFKPSEVTP LSALKLAEIY TEAGVPAGVF NVVQGDGSVG ALLSAHPGIA KVSFTGGVET GKKVMSLAGA SSLKEVTMEL GGKSPLIVFD DADLDRAADI AVTANFFSAG QVCTNGTRVF VQQAVKDAFV ERVLARVARI RVGKPSDSDT NFGPLASAAQ LDKVLGYIDS GKAEGAKLLA GGARLVNDHF ASGQYVAPTV FGDCRDDMRI VREEIFGPVM SILSFETEDE AIARANATDY GLAAGVVTEN LSRAHRAIHR LEAGICWINT WGESPAEMPV GGYKQSGVGR ENGITTLEHY TRIKSVQV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gggtctgcag cgcctttaca tcgccggcgc ccacgccgac gccacgagcg gcaagacgtt cgatacgttc gatcccgcga cgggcgaatt gctcgcgcgc gttcagcagg cgagcgccga cgatgtcgac cgcgcggtcg catccgcgcg cgaaggccag cgcgaatggg ccgcgatgac cgcgatgcag cgctcgcgca tcctgcgtcg cgcggtcgaa ttgctgcgcg agcgcaacga cgcgctcgcc gagctcgaga tgcgcgacac gggcaagccg atcgcggaaa cgcgcgccgt cgacatcgtc acgggcgccg acgtgatcga gtactacgcg ggcctcgcga ccgcgatcga aggtctgcag gtgccgctgc gcccggaatc gttcgtctac acgcggcgcg agccgctcgg cgtgtgcgcg ggcatcggcg catggaacta cccgatccag atcgcctgct ggaagagcgc gcccgcgctc gccgccggca acgcgatgat cttcaagccg agcgaagtca cgccgctgtc cgcgctcaag ctcgcggaga tctacaccga ggcgggagtg cccgccggcg tgttcaacgt cgtgcagggc gacggctcgg tcggcgcgct gctcagcgcg cacccgggca tcgcgaaggt atcgtttacg ggcggcgtcg aaaccggcaa gaaggtgatg tcgctcgcgg gcgcatcgtc gctgaaggaa gtgacgatgg aactgggcgg caaatcgccg ctcatcgtgt tcgacgacgc cgatctcgac cgcgcggccg acatcgccgt caccgcgaac ttcttcagcg cgggccaggt gtgcacgaac ggcacgcgcg tgttcgtcca gcaagcggtg aaggacgcgt tcgtcgagcg cgtgctcgcg cgcgtcgcac ggattcgggt cggcaaacct tcggattccg acacgaattt cggaccgctc gcgagcgccg cccagctcga caaggtgctc ggctacatcg acagcggcaa ggccgaaggc gcgaagctgc tcgcgggcgg cgcgcgcctc gtgaacgatc acttcgcgag cggccagtac gttgcgccga ccgtgttcgg cgattgccgc gacgacatga ggatcgtccg cgaagagatt ttcgggccgg tgatgagcat cctgtcgttc gaaacggaag acgaagcgat cgcgcgcgcg aacgcgaccg actacggcct cgcggcgggc gtcgtcaccg agaacctgtc gcgcgcgcac cgcgcgatcc atcgcctcga agccggcatc tgctggatca acacgtgggg cgaatcgccc gccgagatgc cggttggcgg gtataagcaa tccggcgtcg gacgcgagaa cggcatcacg acgctcgagc actacactcg aatcaaatcg gtgcaggtca aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a
Details for BupsA.00020.b.A5.GE27918
| HARVESTED ON: | 11/24/2009 |
| SEQUENCED ON: | 12/3/2009 |
| EXPECTED MW: | 53kDa |
| OBSERVED MW: | 50kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Few (1-10) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | No Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAGAHADAT SGKTFDTFDP ATGELLARVQ QASADDVDRA VASAREGQRE WAAMTAMQRS RILRRAVELL RERNDALAEL EMRDTGKPIA ETRAVDIVTG ADVIEYYAGL ATAIEGLQVP LRPESFVYTR REPLGVCAGI GAWNYPIQIA CWKSAPALAA GNAMIFKPSE VTPLSALKLA EIYTEAGVPA GVFNVVQGDG SVGALLSAHP GIAKVSFTGG VETGKKVMSL AGASSLKEVT MELGGKSPLI VFDDADLDRA ADIAVTANFF SAGQVCTNGT RVFVQQAVKD AFVERVLARV ARIRVGKPSD SDTNFGPLAS AAQLDKVLGY IDSGKAEGAK LLAGGARLVN DHFASGQYVA PTVFGDCRDD MRIVREEIFG PVMSILSFET EDEAIARANA TDYGLAAGVV TENLSRAHRA IHRLEAGICW INTWGESPAE MPVGGYKQSG VGRENGITTL EHYTRIKSVQ VELGRYQPVF
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggccggcgcc cacgccgacg ccacgagcgg caagacgttc gatacgttcg atcccgcgac gggcgaattg ctcgcgcgcg ttcagcaggc gagcgccgac gatgtcgacc gcgcggtcgc atccgcgcgc gaaggccagc gcgaatgggc cgcgatgacc gcgatgcagc gctcgcgcat cctgcgtcgc gcggtcgaat tgctgcgcga gcgcaacgac gcgctcgccg agctcgagat gcgcgacacg ggcaagccga tcgcggaaac gcgcgccgtc gacatcgtca cgggcgccga cgtgatcgag tactacgcgg gcctcgcgac cgcgatcgaa ggtctgcagg tgccgctgcg cccggaatcg ttcgtctaca cgcggcgcga gccgctcggc gtgtgcgcgg gcatcggcgc atggaactac ccgatccaga tcgcctgctg gaagagcgcg cccgcgctcg ccgccggcaa cgcgatgatc ttcaagccga gcgaagtcac gccgctgtcc gcgctcaagc tcgcggagat ctacaccgag gcgggagtgc ccgccggcgt gttcaacgtc gtgcagggcg acggctcggt cggcgcgctg ctcagcgcgc acccgggcat cgcgaaggta tcgtttacgg gcggcgtcga aaccggcaag aaggtgatgt cgctcgcggg cgcatcgtcg ctgaaggaag tgacgatgga actgggcggc aaatcgccgc tcatcgtgtt cgacgacgcc gatctcgacc gcgcggccga catcgccgtc accgcgaact tcttcagcgc gggccaggtg tgcacgaacg gcacgcgcgt gttcgtccag caagcggtga aggacgcgtt cgtcgagcgc gtgctcgcgc gcgtcgcacg gattcgggtc ggcaaacctt cggattccga cacgaatttc ggaccgctcg cgagcgccgc ccagctcgac aaggtgctcg gctacatcga cagcggcaag gccgaaggcg cgaagctgct cgcgggcggc gcgcgcctcg tgaacgatca cttcgcgagc ggccagtacg ttgcgccgac cgtgttcggc gattgccgcg acgacatgag gatcgtccgc gaagagattt tcgggccggt gatgagcatc ctgtcgttcg aaacggaaga cgaagcgatc gcgcgcgcga acgcgaccga ctacggcctc gcggcgggcg tcgtcaccga gaacctgtcg cgcgcgcacc gcgcgatcca tcgcctcgaa gccggcatct gctggatcaa cacgtggggc gaatcgcccg ccgagatgcc ggttggcggg tataagcaat ccggcgtcgg acgcgagaac ggcatcacga cgctcgagca ctacactcga atcaaatcgg tgcaggtcga gctcggccgc tatcaaccgg tgttcaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for BupsA.00020.b.A6.GE27919
| HARVESTED ON: | 11/24/2009 |
| SEQUENCED ON: | 12/3/2009 |
| EXPECTED MW: | 52kDa |
| OBSERVED MW: | 50kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | No Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 95 |
Validated AA Sequence
MAGAHADATS GKTFDTFDPA TGELLARVQQ ASADDVDRAV ASAREGQREW AAMTAMQRSR ILRRAVELLR ERNDALAELE MRDTGKPIAE TRAVDIVTGA DVIEYYAGLA TAIEGLQVPL RPESFVYTRR EPLGVCAGIG AWNYPIQIAC WKSAPALAAG NAMIFKPSEV TPLSALKLAE IYTEAGVPAG VFNVVQGDGS VGALLSAHPG IAKVSFTGGV ETGKKVMSLA GASSLKEVTM ELGGKSPLIV FDDADLDRAA DIAVTANFFS AGQVCTNGTR VFVQQAVKDA FVERVLARVA RIRVGKPSDS DTNFGPLASA AQLDKVLGYI DSGKAEGAKL LAGGARLVND HFASGQYVAP TVFGDCRDDM RIVREEIFGP VMSILSFETE DEAIARANAT DYGLAAGVVT ENLSRAHRAI HRLEAGICWI NTWGESPAEM PVGGYKQSGV GRENGITTLE HYTRIKS
Validated NT Sequence
ccctggaagc tcagacccag ggtcctggtt cgatggccgg cgcccacgcc gacgccacga gcggcaagac gttcgatacg ttcgatcccg cgacgggcga attgctcgcg cgcgttcagc aggcgagcgc cgacgatgtc gaccgcgcgg tcgcatccgc gcgcgaaggc cagcgcgaat gggccgcgat gaccgcgatg cagcgctcgc gcatcctgcg tcgcgcggtc gaattgctgc gcgagcgcaa cgacgcgctc gccgagctcg agatgcgcga cacgggcaag ccgatcgcgg aaacgcgcgc cgtcgacatc gtcacgggcg ccgacgtgat cgagtactac gcgggcctcg cgaccgcgat cgaaggtctg caggtgccgc tgcgcccgga atcgttcgtc tacacgcggc gcgagccgct cggcgtgtgc gcgggcatcg gcgcatggaa ctacccgatc cagatcgcct gctggaagag cgcgcccgcg ctcgccgccg gcaacgcgat gatcttcaag ccgagcgaag tcacgccgct gtccgcgctc aagctcgcgg agatctacac cgaggcggga gtgcccgccg gcgtgttcaa cgtcgtgcag ggcgacggct cggtcggcgc gctgctcagc gcgcacccgg gcatcgcgaa ggtatcgttt acgggcggcg tcgaaaccgg caagaaggtg atgtcgctcg cgggcgcatc gtcgctgaag gaagtgacga tggaactggg cggcaaatcg ccgctcatcg tgttcgacga cgccgatctc gaccgcgcgg ccgacatcgc cgtcaccgcg aacttcttca gcgcgggcca ggtgtgcacg aacggcacgc gcgtgttcgt ccagcaagcg gtgaaggacg cgttcgtcga gcgcgtgctc gcgcgcgtcg cacggattcg ggtcggcaaa ccttcggatt ccgacacgaa tttcggaccg ctcgcgagcg ccgcccagct cgacaaggtg ctcggctaca tcgacagcgg caaggccgaa ggcgcgaagc tgctcgcggg cggcgcgcgc ctcgtgaacg atcacttcgc gagcggccag tacgttgcgc cgaccgtgtt cggcgattgc cgcgacgaca tgaggatcgt ccgcgaagag attttcgggc cggtgatgag catcctgtcg ttcgaaacgg aagacgaagc gatcgcgcgc gcgaacgcga ccgactacgg cctcgcggcg ggcgtcgtca ccgagaacct gtcgcgcgcg caccgcgcga tccatcgcct cgaagccggc atctgctgga tcaacacgtg gggcgaatcg cccgccgaga tgccggttgg cgggtataag caatccggcg tcggacgcga gaacggcatc acgacgctcg agcactacac tcgaatcaaa tcggtg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAGAHADAT SGKTFDTFDP ATGELLARVQ QASADDVDRA VASAREGQRE WAAMTAMQRS RILRRAVELL RERNDALAEL EMRDTGKPIA ETRAVDIVTG ADVIEYYAGL ATAIEGLQVP LRPESFVYTR REPLGVCAGI GAWNYPIQIA CWKSAPALAA GNAMIFKPSE VTPLSALKLA EIYTEAGVPA GVFNVVQGDG SVGALLSAHP GIAKVSFTGG VETGKKVMSL AGASSLKEVT MELGGKSPLI VFDDADLDRA ADIAVTANFF SAGQVCTNGT RVFVQQAVKD AFVERVLARV ARIRVGKPSD SDTNFGPLAS AAQLDKVLGY IDSGKAEGAK LLAGGARLVN DHFASGQYVA PTVFGDCRDD MRIVREEIFG PVMSILSFET EDEAIARANA TDYGLAAGVV TENLSRAHRA IHRLEAGICW INTWGESPAE MPVGGYKQSG VGRENGITTL EHYTRIKSVQ V
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggccggcgcc cacgccgacg ccacgagcgg caagacgttc gatacgttcg atcccgcgac gggcgaattg ctcgcgcgcg ttcagcaggc gagcgccgac gatgtcgacc gcgcggtcgc atccgcgcgc gaaggccagc gcgaatgggc cgcgatgacc gcgatgcagc gctcgcgcat cctgcgtcgc gcggtcgaat tgctgcgcga gcgcaacgac gcgctcgccg agctcgagat gcgcgacacg ggcaagccga tcgcggaaac gcgcgccgtc gacatcgtca cgggcgccga cgtgatcgag tactacgcgg gcctcgcgac cgcgatcgaa ggtctgcagg tgccgctgcg cccggaatcg ttcgtctaca cgcggcgcga gccgctcggc gtgtgcgcgg gcatcggcgc atggaactac ccgatccaga tcgcctgctg gaagagcgcg cccgcgctcg ccgccggcaa cgcgatgatc ttcaagccga gcgaagtcac gccgctgtcc gcgctcaagc tcgcggagat ctacaccgag gcgggagtgc ccgccggcgt gttcaacgtc gtgcagggcg acggctcggt cggcgcgctg ctcagcgcgc acccgggcat cgcgaaggta tcgtttacgg gcggcgtcga aaccggcaag aaggtgatgt cgctcgcggg cgcatcgtcg ctgaaggaag tgacgatgga actgggcggc aaatcgccgc tcatcgtgtt cgacgacgcc gatctcgacc gcgcggccga catcgccgtc accgcgaact tcttcagcgc gggccaggtg tgcacgaacg gcacgcgcgt gttcgtccag caagcggtga aggacgcgtt cgtcgagcgc gtgctcgcgc gcgtcgcacg gattcgggtc ggcaaacctt cggattccga cacgaatttc ggaccgctcg cgagcgccgc ccagctcgac aaggtgctcg gctacatcga cagcggcaag gccgaaggcg cgaagctgct cgcgggcggc gcgcgcctcg tgaacgatca cttcgcgagc ggccagtacg ttgcgccgac cgtgttcggc gattgccgcg acgacatgag gatcgtccgc gaagagattt tcgggccggt gatgagcatc ctgtcgttcg aaacggaaga cgaagcgatc gcgcgcgcga acgcgaccga ctacggcctc gcggcgggcg tcgtcaccga gaacctgtcg cgcgcgcacc gcgcgatcca tcgcctcgaa gccggcatct gctggatcaa cacgtggggc gaatcgcccg ccgagatgcc ggttggcggg tataagcaat ccggcgtcgg acgcgagaac ggcatcacga cgctcgagca ctacactcga atcaaatcgg tgcaggtcaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for BupsA.00020.b.AE1.GE43326
| HARVESTED ON: | 7/31/2019 |
| SEQUENCED ON: | 8/7/2019 |
| EXPECTED MW: | 53kDa |
| OBSERVED MW: | 53kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 59 |
| PERCENT COVERAGE: | 91 |
Validated AA Sequence
MSVYGLQRLY IAGAHADATS GKTFDTFDPA TGELLARVQQ ASADDVDRAV ASAREGQREW AAMTAMQRSR ILRRAVELLR ERNDALAELE MRDTGKPIAE TRAVDIVTGA DVIEYYAGLA TAIEGLQVPL RPESFVYTRR EPLGVCAGIG AWNYPIQIAC WKSAPALAAG NAMIFKPSEV TPLSALKLAE IYTEAGVPAG VFXVVQGDGS VGALLSAHPG IAKVSFTGGV ETGKXGDVAR GRIVAEGSDD GTGRQIAAHR VRRRRSRPRG RHRRHRELLQ RGPGVHERHA RVRPASGEGR VRRARARARR XDXGRQTFGX XHEFRTARER RPAXQGARXH RQRQGRRREA ARGRRAPRER SLXERPVRCX DRVXXLPXRH EDRPRRDFRA GDEHPVVXNG RXSDRARERD RXRPRGGRXH REPVARAPRD PSPRSRHLXD QHVGXIARRX XGXRV
Validated NT Sequence
tccgttaatg atgatgatgg tggtgcccan cgaacaccgg ttgatagcgg ccgagctcga cctgcaccga tttgattcna gtgtantgct cgagcgtcgt gatgccgttc tcgcgtccga cgccggattg cttatacccg ccnaccggnn tctcggcggg cgattcnccc cacgtgttga tccancagat gccggcttcg aggcgatgga tcgcgcggtg cgcgcgcgac aggttctcgg tgacnacgcc cgccgcgagg ccgtantcgg tcgcgttcgc gcgcgcgatc gcttcntctt ccgtttcnaa cgacaggatg ctcatcaccg gcccgaaaat ctcttcgcgg acgatcctca tgtcgncncg gcaatcnccn aacacggtcg gngcancgta ctggccgctc gcnaagtgat cgttcacgag gcgcgcgccg cccgcgagca gcttcncgcc ttcggccttg ccgctgtcga tgtanncgag caccttgtcn agctgggcgg cgctcgcgag cggtccgaaa ttcgtgtcnn antccgaang tttgccgacc cnaatccntg cgacgcgcgc gagcacgcgc tcgacgaacg cgtccttcac cgcttgctgg acgaacacgc gcgtgccgtt cgtgcacacc tggcccgcgc tgaagaagtt cgcggtgacg gcgatgtcgn ccgcgcggtc gagatcggcg tcgtcgaaca cgatgagcgg cgatttgccg cccagttcca tcgtcacttc cttcagcgac gatgcgcccg cgagcgacat caccnttctt gccggtttcg acgccgcccg taaacgatac cttcgcgatg cccgggtgcg cgctgagcag cgcgccgacc gagccgtcgc cctgcacgac gtngaacacg ccggcgggca ctcccgcctc ggtgtagatc tccgcgagct tgagcgcgga cagcggcgtg acttcgctcg gcttgaagat catcgcgttg ccggcggcga gcgcgggcgc gctcttccag caggcgatct ggatcgggta gttccatgcg ccgatgcccg cgcacacgcc gagcggctcg cgccgcgtgt agacgaacga ttccgggcgc agcggcacct gcagaccttc gatcgcggtc gcgaggcccg cgtagtactc gatcacgtcg gcgcccgtga cgatgtcgac ggcgcgcgtt tccgcgatcg gcttgcccgt gtcgcgcatc tcgagctcgg cgagcgcgtc gttgcgctcg cgcagcaatt cgaccgcgcg acgcaggatg cgcgagcgct gcatcgcggt catcgcggcc cattcgcgct ggccttcgcg cgcggatgcg accgcgcggt cgacatcgtc ggcgctcgcc tgctgaacgc gcgcgagcaa ttcgcccgtc gcgggatcga acgtatcgaa cgtcttgccg ctcgtggcgt cggcgtgggc gccggcgatg taaaggcgct gcagaccgta tacggacatg ggagaga
Expected Protein Sequence
MSVYGLQRLY IAGAHADATS GKTFDTFDPA TGELLARVQQ ASADDVDRAV ASAREGQREW AAMTAMQRSR ILRRAVELLR ERNDALAELE MRDTGKPIAE TRAVDIVTGA DVIEYYAGLA TAIEGLQVPL RPESFVYTRR EPLGVCAGIG AWNYPIQIAC WKSAPALAAG NAMIFKPSEV TPLSALKLAE IYTEAGVPAG VFNVVQGDGS VGALLSAHPG IAKVSFTGGV ETGKKVMSLA GASSLKEVTM ELGGKSPLIV FDDADLDRAA DIAVTANFFS AGQVCTNGTR VFVQQAVKDA FVERVLARVA RIRVGKPSDS DTNFGPLASA AQLDKVLGYI DSGKAEGAKL LAGGARLVND HFASGQYVAP TVFGDCRDDM RIVREEIFGP VMSILSFETE DEAIARANAT DYGLAAGVVT ENLSRAHRAI HRLEAGICWI NTWGESPAEM PVGGYKQSGV GRENGITTLE HYTRIKSVQV ELGRYQPVFG HHHHHH
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atttgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat cgatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt tgtttaactt taagaaggag tctctcccat gtccgtatac ggtctgcagc gcctttacat cgccggcgcc cacgccgacg ccacgagcgg caagacgttc gatacgttcg atcccgcgac gggcgaattg ctcgcgcgcg ttcagcaggc gagcgccgac gatgtcgacc gcgcggtcgc atccgcgcgc gaaggccagc gcgaatgggc cgcgatgacc gcgatgcagc gctcgcgcat cctgcgtcgc gcggtcgaat tgctgcgcga gcgcaacgac gcgctcgccg agctcgagat gcgcgacacg ggcaagccga tcgcggaaac gcgcgccgtc gacatcgtca cgggcgccga cgtgatcgag tactacgcgg gcctcgcgac cgcgatcgaa ggtctgcagg tgccgctgcg cccggaatcg ttcgtctaca cgcggcgcga gccgctcggc gtgtgcgcgg gcatcggcgc atggaactac ccgatccaga tcgcctgctg gaagagcgcg cccgcgctcg ccgccggcaa cgcgatgatc ttcaagccga gcgaagtcac gccgctgtcc gcgctcaagc tcgcggagat ctacaccgag gcgggagtgc ccgccggcgt gttcaacgtc gtgcagggcg acggctcggt cggcgcgctg ctcagcgcgc acccgggcat cgcgaaggta tcgtttacgg gcggcgtcga aaccggcaag aaggtgatgt cgctcgcggg cgcatcgtcg ctgaaggaag tgacgatgga actgggcggc aaatcgccgc tcatcgtgtt cgacgacgcc gatctcgacc gcgcggccga catcgccgtc accgcgaact tcttcagcgc gggccaggtg tgcacgaacg gcacgcgcgt gttcgtccag caagcggtga aggacgcgtt cgtcgagcgc gtgctcgcgc gcgtcgcacg gattcgggtc ggcaaacctt cggattccga cacgaatttc ggaccgctcg cgagcgccgc ccagctcgac aaggtgctcg gctacatcga cagcggcaag gccgaaggcg cgaagctgct cgcgggcggc gcgcgcctcg tgaacgatca cttcgcgagc ggccagtacg ttgcgccgac cgtgttcggc gattgccgcg acgacatgag gatcgtccgc gaagagattt tcgggccggt gatgagcatc ctgtcgttcg aaacggaaga cgaagcgatc gcgcgcgcga acgcgaccga ctacggcctc gcggcgggcg tcgtcaccga gaacctgtcg cgcgcgcacc gcgcgatcca tcgcctcgaa gccggcatct gctggatcaa cacgtggggc gaatcgcccg ccgagatgcc ggttggcggg tataagcaat ccggcgtcgg acgcgagaac ggcatcacga cgctcgagca ctacactcga atcaaatcgg tgcaggtcga gctcggccgc tatcaaccgg tgttcgggca ccaccatcat catcattaac ggatccgaat tcgagctccg tcgacaagct tgcggccgca ctcgagcacc accaccacca ccactgagat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gat
Details for BupsA.00020.b.B1.PS00017
| PURIFICATION DATe: | 5/28/2008 |
| CONCENTRATION: | 44.5mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 5 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | 21 |
| PERCENT COVERAGE: | 86 |
Protocol Notes
notes unavailable
Validated AA Sequence
MDRAVRARQV LGDDARREAV VGRVRARDRF VFRFERQDAH HRPENLFADD PHVVAAIAEH GRRNVLAARE VIVHEARAAR EQLRAFGLAA VDVAEHLVEL GGARERSEIR VGIRRFADPN PCDAREHALD ERVLHRLLDE HARAVRAHLA RAEEVRGDGD VGRAVEIGVV EHDERRFAAQ FHRHFLQRRC ARERHHLLAG FDAARKRYLR DARVRAEQRA DRAVALHDVE HAGGHSRLGV DLRELERGQR RDFARLEDHR VAGGERGRAL PAGDLDRVVP CADARAHAER LAPRVDERFR AQRHLQTFDR GREARVVLDH VGARDDVDGA RFRDRLARVA HLELGERVVA LAQQFDRATQ DARALHRGHR GPFALAFARG CDRAVDIVGA RLLNAREQFA RRGIERIERL AARGVGVGAG DVKALQTVYG
Validated NT Sequence
gagctcgacc tgcaccgatt tgattcgagt gtagtgctcg agcgtcgtga tgccgttctc gcgtccgacg ccggattgct tatacccgcc aaccggcatc tcggcgggcg attcgcccca cgtgttgatc cagcagatgc cggcttcgag gcgatggatc gcgcggtgcg cgcgcgacag gttctcggtg acgacgcccg ccgcgaggcc gtagtcggtc gcgttcgcgc gcgcgatcgc ttcgtcttcc gtttcgaacg acaggatgct catcaccggc ccgaaaatct cttcgcggac gatcctcatg tcgtcgcggc aatcgccgaa cacggtcggc gcaacgtact ggccgctcgc gaagtgatcg ttcacgaggc gcgcgccgcc cgcgagcagc ttcgcgcctt cggccttgcc gctgtcgatg tagccgagca ccttgtcgag ctgggcggcg ctcgcgagcg gtccgaaatt cgtgtcggaa tccgaaggtt tgccgacccg aatccgtgcg acgcgcgcga gcacgcgctc gacgaacgcg tccttcaccg cttgctggac gaacacgcgc gtgccgttcg tgcacacctg gcccgcgctg aagaagttcg cggtgacggc gatgtcggcc gcgcggtcga gatcggcgtc gtcgaacacg atgagcggcg atttgccgcc cagttccatc gtcacttcct tcagcgacga tgcgcccgcg agcgacatca ccttcttgcc ggtttcgacg ccgcccgtaa acgatacctt cgcgatgccc gggtgcgcgc tgagcagcgc gccgaccgag ccgtcgccct gcacgacgtt gaacacgccg gcgggcactc ccgcctcggt gtagatctcc gcgagcttga gcgcggacag cggcgtgact tcgctcggct tgaagatcat cgcgttgccg gcggcgagcg cgggcgcgct cttccagcag gcgatctgga tcgggtagtt ccatgcgccg atgcccgcgc acacgccgag cggctcgcgc cgcgtgtaga cgaacgattc cgggcgcagc ggcacctgca gaccttcgat cgcggtcgcg aggcccgcgt agtactcgat cacgtcggcg cccgtgacga tgtcgacggc gcgcgtttcc gcgatcggct tgcccgtgtc gcgcatctcg agctcggcga gcgcgtcgtt gcgctcgcgc agcaattcga ccgcgcgacg caggatgcgc gagcgctgca tcgcggtcat cgcggcccat tcgcgctggc cttcgcgcgc ggatgcgacc gcgcggtcga catcgtcggc gctcgcctgc tgaacgcgcg cgagcaattc gcccgtcgcg ggatcgaacg tatcgaacgt cttgccgctc gtggcgtcgg cgtgggcgcc ggcgatgtaa aggcgctgca gaccgtatac ggac
Expressed Protein Sequence
MAHHHHHHMS VYGLQRLYIA GAHADATSGK TFDTFDPATG ELLARVQQAS ADDVDRAVAS AREGQREWAA MTAMQRSRIL RRAVELLRER NDALAELEMR DTGKPIAETR AVDIVTGADV IEYYAGLATA IEGLQVPLRP ESFVYTRREP LGVCAGIGAW NYPIQIACWK SAPALAAGNA MIFKPSEVTP LSALKLAEIY TEAGVPAGVF NVVQGDGSVG ALLSAHPGIA KVSFTGGVET GKKVMSLAGA SSLKEVTMEL GGKSPLIVFD DADLDRAADI AVTANFFSAG QVCTNGTRVF VQQAVKDAFV ERVLARVARI RVGKPSDSDT NFGPLASAAQ LDKVLGYIDS GKAEGAKLLA GGARLVNDHF ASGQYVAPTV FGDCRDDMRI VREEIFGPVM SILSFETEDE AIARANATDY GLAAGVVTEN LSRAHRAIHR LEAGICWINT WGESPAEMPV GGYKQSGVGR ENGITTLEHY TRIKSVQVEL GRYQPVF
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTCCG TATACGGTCT GCAGCGCCTT TACATCGCCG GCGCCCACGC CGACGCCACG AGCGGCAAGA CGTTCGATAC GTTCGATCCC GCGACGGGCG AATTGCTCGC GCGCGTTCAG CAGGCGAGCG CCGACGATGT CGACCGCGCG GTCGCATCCG CGCGCGAAGG CCAGCGCGAA TGGGCCGCGA TGACCGCGAT GCAGCGCTCG CGCATCCTGC GTCGCGCGGT CGAATTGCTG CGCGAGCGCA ACGACGCGCT CGCCGAGCTC GAGATGCGCG ACACGGGCAA GCCGATCGCG GAAACGCGCG CCGTCGACAT CGTCACGGGC GCCGACGTGA TCGAGTACTA CGCGGGCCTC GCGACCGCGA TCGAAGGTCT GCAGGTGCCG CTGCGCCCGG AATCGTTCGT CTACACGCGG CGCGAGCCGC TCGGCGTGTG CGCGGGCATC GGCGCATGGA ACTACCCGAT CCAGATCGCC TGCTGGAAGA GCGCGCCCGC GCTCGCCGCC GGCAACGCGA TGATCTTCAA GCCGAGCGAA GTCACGCCGC TGTCCGCGCT CAAGCTCGCG GAGATCTACA CCGAGGCGGG AGTGCCCGCC GGCGTGTTCA ACGTCGTGCA GGGCGACGGC TCGGTCGGCG CGCTGCTCAG CGCGCACCCG GGCATCGCGA AGGTATCGTT TACGGGCGGC GTCGAAACCG GCAAGAAGGT GATGTCGCTC GCGGGCGCAT CGTCGCTGAA GGAAGTGACG ATGGAACTGG GCGGCAAATC GCCGCTCATC GTGTTCGACG ACGCCGATCT CGACCGCGCG GCCGACATCG CCGTCACCGC GAACTTCTTC AGCGCGGGCC AGGTGTGCAC GAACGGCACG CGCGTGTTCG TCCAGCAAGC GGTGAAGGAC GCGTTCGTCG AGCGCGTGCT CGCGCGCGTC GCACGGATTC GGGTCGGCAA ACCTTCGGAT TCCGACACGA ATTTCGGACC GCTCGCGAGC GCCGCCCAGC TCGACAAGGT GCTCGGCTAC ATCGACAGCG GCAAGGCCGA AGGCGCGAAG CTGCTCGCGG GCGGCGCGCG CCTCGTGAAC GATCACTTCG CGAGCGGCCA GTACGTTGCG CCGACCGTGT TCGGCGATTG CCGCGACGAC ATGAGGATCG TCCGCGAAGA GATTTTCGGG CCGGTGATGA GCATCCTGTC GTTCGAAACG GAAGACGAAG CGATCGCGCG CGCGAACGCG ACCGACTACG GCCTCGCGGC GGGCGTCGTC ACCGAGAACC TGTCGCGCGC GCACCGCGCG ATCCATCGCC TCGAAGCCGG CATCTGCTGG ATCAACACGT GGGGCGAATC GCCCGCCGAG ATGCCGGTTG GCGGGTATAA GCAATCCGGC GTCGGACGCG AGAACGGCAT CACGACGCTC GAGCACTACA CTCGAATCAA ATCGGTGCAG GTCGAGCTCG GCCGCTATCA ACCGGTGTTC TAATGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT
Details for BupsA.00020.b.AE1.PS38619
| PURIFICATION DATe: | 11/5/2019 |
| CONCENTRATION: | 34.72mg/ml |
| OBSERVED MW: | 53kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | SEC: 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 9 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 59 |
| PERCENT COVERAGE: | 91 |
Protocol Notes
notes unavailable
Validated AA Sequence
MSVYGLQRLY IAGAHADATS GKTFDTFDPA TGELLARVQQ ASADDVDRAV ASAREGQREW AAMTAMQRSR ILRRAVELLR ERNDALAELE MRDTGKPIAE TRAVDIVTGA DVIEYYAGLA TAIEGLQVPL RPESFVYTRR EPLGVCAGIG AWNYPIQIAC WKSAPALAAG NAMIFKPSEV TPLSALKLAE IYTEAGVPAG VFXVVQGDGS VGALLSAHPG IAKVSFTGGV ETGKXGDVAR GRIVAEGSDD GTGRQIAAHR VRRRRSRPRG RHRRHRELLQ RGPGVHERHA RVRPASGEGR VRRARARARR XDXGRQTFGX XHEFRTARER RPAXQGARXH RQRQGRRREA ARGRRAPRER SLXERPVRCX DRVXXLPXRH EDRPRRDFRA GDEHPVVXNG RXSDRARERD RXRPRGGRXH REPVARAPRD PSPRSRHLXD QHVGXIARRX XGXRV
Validated NT Sequence
tccgttaatg atgatgatgg tggtgcccan cgaacaccgg ttgatagcgg ccgagctcga cctgcaccga tttgattcna gtgtantgct cgagcgtcgt gatgccgttc tcgcgtccga cgccggattg cttatacccg ccnaccggnn tctcggcggg cgattcnccc cacgtgttga tccancagat gccggcttcg aggcgatgga tcgcgcggtg cgcgcgcgac aggttctcgg tgacnacgcc cgccgcgagg ccgtantcgg tcgcgttcgc gcgcgcgatc gcttcntctt ccgtttcnaa cgacaggatg ctcatcaccg gcccgaaaat ctcttcgcgg acgatcctca tgtcgncncg gcaatcnccn aacacggtcg gngcancgta ctggccgctc gcnaagtgat cgttcacgag gcgcgcgccg cccgcgagca gcttcncgcc ttcggccttg ccgctgtcga tgtanncgag caccttgtcn agctgggcgg cgctcgcgag cggtccgaaa ttcgtgtcnn antccgaang tttgccgacc cnaatccntg cgacgcgcgc gagcacgcgc tcgacgaacg cgtccttcac cgcttgctgg acgaacacgc gcgtgccgtt cgtgcacacc tggcccgcgc tgaagaagtt cgcggtgacg gcgatgtcgn ccgcgcggtc gagatcggcg tcgtcgaaca cgatgagcgg cgatttgccg cccagttcca tcgtcacttc cttcagcgac gatgcgcccg cgagcgacat caccnttctt gccggtttcg acgccgcccg taaacgatac cttcgcgatg cccgggtgcg cgctgagcag cgcgccgacc gagccgtcgc cctgcacgac gtngaacacg ccggcgggca ctcccgcctc ggtgtagatc tccgcgagct tgagcgcgga cagcggcgtg acttcgctcg gcttgaagat catcgcgttg ccggcggcga gcgcgggcgc gctcttccag caggcgatct ggatcgggta gttccatgcg ccgatgcccg cgcacacgcc gagcggctcg cgccgcgtgt agacgaacga ttccgggcgc agcggcacct gcagaccttc gatcgcggtc gcgaggcccg cgtagtactc gatcacgtcg gcgcccgtga cgatgtcgac ggcgcgcgtt tccgcgatcg gcttgcccgt gtcgcgcatc tcgagctcgg cgagcgcgtc gttgcgctcg cgcagcaatt cgaccgcgcg acgcaggatg cgcgagcgct gcatcgcggt catcgcggcc cattcgcgct ggccttcgcg cgcggatgcg accgcgcggt cgacatcgtc ggcgctcgcc tgctgaacgc gcgcgagcaa ttcgcccgtc gcgggatcga acgtatcgaa cgtcttgccg ctcgtggcgt cggcgtgggc gccggcgatg taaaggcgct gcagaccgta tacggacatg ggagaga
Expressed Protein Sequence
MSVYGLQRLY IAGAHADATS GKTFDTFDPA TGELLARVQQ ASADDVDRAV ASAREGQREW AAMTAMQRSR ILRRAVELLR ERNDALAELE MRDTGKPIAE TRAVDIVTGA DVIEYYAGLA TAIEGLQVPL RPESFVYTRR EPLGVCAGIG AWNYPIQIAC WKSAPALAAG NAMIFKPSEV TPLSALKLAE IYTEAGVPAG VFNVVQGDGS VGALLSAHPG IAKVSFTGGV ETGKKVMSLA GASSLKEVTM ELGGKSPLIV FDDADLDRAA DIAVTANFFS AGQVCTNGTR VFVQQAVKDA FVERVLARVA RIRVGKPSDS DTNFGPLASA AQLDKVLGYI DSGKAEGAKL LAGGARLVND HFASGQYVAP TVFGDCRDDM RIVREEIFGP VMSILSFETE DEAIARANAT DYGLAAGVVT ENLSRAHRAI HRLEAGICWI NTWGESPAEM PVGGYKQSGV GRENGITTLE HYTRIKSVQV ELGRYQPVFG HHHHHH
Full NT Sequence (Expression Vector + Insert)
TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACATTAACGC TTACAATTTA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATTTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAG ATCCCGGTGC CTAATGAGTG AGCTAACTTA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC CAGGGTGGTT TTTCTTTTCA CCAGTGAGAC GGGCAACAGC TGATTGCCCT TCACCGCCTG GCCCTGAGAG AGTTGCAGCA AGCGGTCCAC GCTGGTTTGC CCCAGCAGGC GAAAATCCTG TTTGATGGTG GTTAACGGCG GGATATAACA TGAGCTGTCT TCGGTATCGT CGTATCCCAC TACCGAGATA TCCGCACCAA CGCGCAGCCC GGACTCGGTA ATGGCGCGCA TTGCGCCCAG CGCCATCTGA TCGTTGGCAA CCAGCATCGC AGTGGGAACG ATGCCCTCAT TCAGCATTTG CATGGTTTGT TGAAAACCGG ACATGGCACT CCAGTCGCCT TCCCGTTCCG CTATCGGCTG AATTTGATTG CGAGTGAGAT ATTTATGCCA GCCAGCCAGA CGCAGACGCG CCGAGACAGA ACTTAATGGG CCCGCTAACA GCGCGATTTG CTGGTGACCC AATGCGACCA GATGCTCCAC GCCCAGTCGC GTACCGTCTT CATGGGAGAA AATAATACTG TTGATGGGTG TCTGGTCAGA GACATCAAGA AATAACGCCG GAACATTAGT GCAGGCAGCT TCCACAGCAA TGGCATCCTG GTCATCCAGC GGATAGTTAA TGATCAGCCC ACTGACGCGT TGCGCGAGAA GATTGTGCAC CGCCGCTTTA CAGGCTTCGA CGCCGCTTCG TTCTACCATC GACACCACCA CGCTGGCACC CAGTTGATCG GCGCGAGATT TAATCGCCGC GACAATTTGC GACGGCGCGT GCAGGGCCAG ACTGGAGGTG GCAACGCCAA TCAGCAACGA CTGTTTGCCC GCCAGTTGTT GTGCCACGCG GTTGGGAATG TAATTCAGCT CCGCCATCGC CGCTTCCACT TTTTCCCGCG TTTTCGCAGA AACGTGGCTG GCCTGGTTCA CCACGCGGGA AACGGTCTGA TAAGAGACAC CGGCATACTC TGCGACATCG TATAACGTTA CTGGTTTCAC ATTCACCACC CTGAATTGAC TCTCTTCCGG GCGCTATCAT GCCATACCGC GAAAGGTTTT GCGCCATTCG ATGGTGTCCG GGATCTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CGATCTCGAT CCCGCGAAAT TAATACGACT CACTATAGGG GAATTGTGAG CGGATAACAA TTCCCCTCTA GAAATAATTT TGTTTAACTT TAAGAAGGAG TCTCTCCCAT GTCCGTATAC GGTCTGCAGC GCCTTTACAT CGCCGGCGCC CACGCCGACG CCACGAGCGG CAAGACGTTC GATACGTTCG ATCCCGCGAC GGGCGAATTG CTCGCGCGCG TTCAGCAGGC GAGCGCCGAC GATGTCGACC GCGCGGTCGC ATCCGCGCGC GAAGGCCAGC GCGAATGGGC CGCGATGACC GCGATGCAGC GCTCGCGCAT CCTGCGTCGC GCGGTCGAAT TGCTGCGCGA GCGCAACGAC GCGCTCGCCG AGCTCGAGAT GCGCGACACG GGCAAGCCGA TCGCGGAAAC GCGCGCCGTC GACATCGTCA CGGGCGCCGA CGTGATCGAG TACTACGCGG GCCTCGCGAC CGCGATCGAA GGTCTGCAGG TGCCGCTGCG CCCGGAATCG TTCGTCTACA CGCGGCGCGA GCCGCTCGGC GTGTGCGCGG GCATCGGCGC ATGGAACTAC CCGATCCAGA TCGCCTGCTG GAAGAGCGCG CCCGCGCTCG CCGCCGGCAA CGCGATGATC TTCAAGCCGA GCGAAGTCAC GCCGCTGTCC GCGCTCAAGC TCGCGGAGAT CTACACCGAG GCGGGAGTGC CCGCCGGCGT GTTCAACGTC GTGCAGGGCG ACGGCTCGGT CGGCGCGCTG CTCAGCGCGC ACCCGGGCAT CGCGAAGGTA TCGTTTACGG GCGGCGTCGA AACCGGCAAG AAGGTGATGT CGCTCGCGGG CGCATCGTCG CTGAAGGAAG TGACGATGGA ACTGGGCGGC AAATCGCCGC TCATCGTGTT CGACGACGCC GATCTCGACC GCGCGGCCGA CATCGCCGTC ACCGCGAACT TCTTCAGCGC GGGCCAGGTG TGCACGAACG GCACGCGCGT GTTCGTCCAG CAAGCGGTGA AGGACGCGTT CGTCGAGCGC GTGCTCGCGC GCGTCGCACG GATTCGGGTC GGCAAACCTT CGGATTCCGA CACGAATTTC GGACCGCTCG CGAGCGCCGC CCAGCTCGAC AAGGTGCTCG GCTACATCGA CAGCGGCAAG GCCGAAGGCG CGAAGCTGCT CGCGGGCGGC GCGCGCCTCG TGAACGATCA CTTCGCGAGC GGCCAGTACG TTGCGCCGAC CGTGTTCGGC GATTGCCGCG ACGACATGAG GATCGTCCGC GAAGAGATTT TCGGGCCGGT GATGAGCATC CTGTCGTTCG AAACGGAAGA CGAAGCGATC GCGCGCGCGA ACGCGACCGA CTACGGCCTC GCGGCGGGCG TCGTCACCGA GAACCTGTCG CGCGCGCACC GCGCGATCCA TCGCCTCGAA GCCGGCATCT GCTGGATCAA CACGTGGGGC GAATCGCCCG CCGAGATGCC GGTTGGCGGG TATAAGCAAT CCGGCGTCGG ACGCGAGAAC GGCATCACGA CGCTCGAGCA CTACACTCGA ATCAAATCGG TGCAGGTCGA GCTCGGCCGC TATCAACCGG TGTTCGGGCA CCACCATCAT CATCATTAAC GGATCCGAAT TCGAGCTCCG TCGACAAGCT TGCGGCCGCA CTCGAGCACC ACCACCACCA CCACTGAGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GAT
Details for BupsA.00020.b.A3.PW27863
| PURIFICATION DATe: | 1/19/2010 |
| CONCENTRATION: | 27.24mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 15 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGLQRLYIA GAHADATSGK TFDTFDPATG ELLARVQQAS ADDVDRAVAS AREGQREWAA MTAMQRSRIL RRAVELLRER NDALAELEMR DTGKPIAETR AVDIVTGADV IEYYAGLATA IEGLQVPLRP ESFVYTRREP LGVCAGIGAW NYPIQIACWK SAPALAAGNA MIFKPSEVTP LSALKLAEIY TEAGVPAGVF NVVQGDGSVG ALLSAHPGIA KVSFTGGVET GKKVMSLAGA SSLKEVTMEL GGKSPLIVFD DADLDRAADI AVTANFFSAG QVCTNGTRVF VQQAVKDAFV ERVLARVARI RVGKPSDSDT NFGPLASAAQ LDKVLGYIDS GKAEGAKLLA GGARLVNDHF ASGQYVAPTV FGDCRDDMRI VREEIFGPVM SILSFETEDE AIARANATDY GLAAGVVTEN LSRAHRAIHR LEAGICWINT WGESPAEMPV GGYKQSGVGR ENGITTLEHY TRIKSVQVEL GRYQPVF
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGGTCTGCAG CGCCTTTACA TCGCCGGCGC CCACGCCGAC GCCACGAGCG GCAAGACGTT CGATACGTTC GATCCCGCGA CGGGCGAATT GCTCGCGCGC GTTCAGCAGG CGAGCGCCGA CGATGTCGAC CGCGCGGTCG CATCCGCGCG CGAAGGCCAG CGCGAATGGG CCGCGATGAC CGCGATGCAG CGCTCGCGCA TCCTGCGTCG CGCGGTCGAA TTGCTGCGCG AGCGCAACGA CGCGCTCGCC GAGCTCGAGA TGCGCGACAC GGGCAAGCCG ATCGCGGAAA CGCGCGCCGT CGACATCGTC ACGGGCGCCG ACGTGATCGA GTACTACGCG GGCCTCGCGA CCGCGATCGA AGGTCTGCAG GTGCCGCTGC GCCCGGAATC GTTCGTCTAC ACGCGGCGCG AGCCGCTCGG CGTGTGCGCG GGCATCGGCG CATGGAACTA CCCGATCCAG ATCGCCTGCT GGAAGAGCGC GCCCGCGCTC GCCGCCGGCA ACGCGATGAT CTTCAAGCCG AGCGAAGTCA CGCCGCTGTC CGCGCTCAAG CTCGCGGAGA TCTACACCGA GGCGGGAGTG CCCGCCGGCG TGTTCAACGT CGTGCAGGGC GACGGCTCGG TCGGCGCGCT GCTCAGCGCG CACCCGGGCA TCGCGAAGGT ATCGTTTACG GGCGGCGTCG AAACCGGCAA GAAGGTGATG TCGCTCGCGG GCGCATCGTC GCTGAAGGAA GTGACGATGG AACTGGGCGG CAAATCGCCG CTCATCGTGT TCGACGACGC CGATCTCGAC CGCGCGGCCG ACATCGCCGT CACCGCGAAC TTCTTCAGCG CGGGCCAGGT GTGCACGAAC GGCACGCGCG TGTTCGTCCA GCAAGCGGTG AAGGACGCGT TCGTCGAGCG CGTGCTCGCG CGCGTCGCAC GGATTCGGGT CGGCAAACCT TCGGATTCCG ACACGAATTT CGGACCGCTC GCGAGCGCCG CCCAGCTCGA CAAGGTGCTC GGCTACATCG ACAGCGGCAA GGCCGAAGGC GCGAAGCTGC TCGCGGGCGG CGCGCGCCTC GTGAACGATC ACTTCGCGAG CGGCCAGTAC GTTGCGCCGA CCGTGTTCGG CGATTGCCGC GACGACATGA GGATCGTCCG CGAAGAGATT TTCGGGCCGG TGATGAGCAT CCTGTCGTTC GAAACGGAAG ACGAAGCGAT CGCGCGCGCG AACGCGACCG ACTACGGCCT CGCGGCGGGC GTCGTCACCG AGAACCTGTC GCGCGCGCAC CGCGCGATCC ATCGCCTCGA AGCCGGCATC TGCTGGATCA ACACGTGGGG CGAATCGCCC GCCGAGATGC CGGTTGGCGG GTATAAGCAA TCCGGCGTCG GACGCGAGAA CGGCATCACG ACGCTCGAGC ACTACACTCG AATCAAATCG GTGCAGGTCG AGCTCGGCCG CTATCAACCG GTGTTCAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA
Details for BupsA.00020.b.A2.PW28611
| PURIFICATION DATe: | 1/19/2010 |
| CONCENTRATION: | 21.13mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 3 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | 98 |
| PERCENT COVERAGE: | 63 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSVYGLQRL YIAGAHADAT SGKTFDTFDP ATGELLARVQ QASADDVDRA VASAREGQRE WAAMTAMQRS RILRRAVELL RERNDALAEL EMRDTGKPIA ETRAVDIVTG ADVIEYYAGL ATAIEGLQVP LRPESFVYTR REPLGVCAGI GAWNYPIQIA CWKSAPALAA GNAMIFKPSE VTPLSALKLA EIYTEAGVPA GVFNVVQGDG SVGALLSAHP GIAXVSFTGG VETGKKVMSL AGASSLKEVT MELGGKSPLI VFDDAXLDRA ADIAVTANFF SAGXXCTNGT RVFVQQ
Validated NT Sequence
tttaagaagg aganatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgtccgt atacggtctg cagcgccttt acatcgccgg cgcccacgcc gacgccacga gcggcaagac gttcgatacg ttcgatcccg cgacgggcga attgctcgcg cgcgttcagc aggcgagcgc cgacgatgtc gaccgcgcgg tcgcatccgc gcgcgaaggc cagcgcgaat gggccgcgat gaccgcgatg cagcgctcgc gcatcctgcg tcgcgcggtc gaattgctgc gcgagcgcaa cgacgcgctc gccgagctcg agatgcgcga cacgggcaag ccgatcgcgg aaacgcgcgc cgtcgacatc gtcacgggcg ccgacgtgat cgagtactac gcgggcctcg cgaccgcgat cgaaggtctg caggtgccgc tgcgcccgga atcgttcgtc tacacgcggc gcgagccgct cggcgtgtgc gcgggcatcg gcgcatggaa ctacccgatc cagatcgcct gctggaagag cgcgcccgcg ctcgccgccg gcaacgcgat gatcttcaag ccgagcgaag tcacgccgct gtccgcgctc aagctcgcgg agatctacac cgaggcggga gtgcccgccg gcgtgttcaa cgtcgtgcag ggcgacggct cggtcggcgc gctgctcagc gcgcacccgg gcatcgcgaa ngtatcgttt acgggcggcg tcgaaaccgg caagaaggtg atgtcgctcg cgggcgcatc gtcgctgaag gaagtgacga tggaactggg cggcaaatcg ccgctcatcg tgttcgacga cgccnatctc gaccgcgcgg ccgacatcgc cgtcaccgcg aacttcttca gcgcgggcca ngngtgcacg aacggcacgc gcgtgttcgt ccagcaagcg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSVYGLQRL YIAGAHADAT SGKTFDTFDP ATGELLARVQ QASADDVDRA VASAREGQRE WAAMTAMQRS RILRRAVELL RERNDALAEL EMRDTGKPIA ETRAVDIVTG ADVIEYYAGL ATAIEGLQVP LRPESFVYTR REPLGVCAGI GAWNYPIQIA CWKSAPALAA GNAMIFKPSE VTPLSALKLA EIYTEAGVPA GVFNVVQGDG SVGALLSAHP GIAKVSFTGG VETGKKVMSL AGASSLKEVT MELGGKSPLI VFDDADLDRA ADIAVTANFF SAGQVCTNGT RVFVQQAVKD AFVERVLARV ARIRVGKPSD SDTNFGPLAS AAQLDKVLGY IDSGKAEGAK LLAGGARLVN DHFASGQYVA PTVFGDCRDD MRIVREEIFG PVMSILSFET EDEAIARANA TDYGLAAGVV TENLSRAHRA IHRLEAGICW INTWGESPAE MPVGGYKQSG VGRENGITTL EHYTRIKSVQ V
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCCGTATAC GGTCTGCAGC GCCTTTACAT CGCCGGCGCC CACGCCGACG CCACGAGCGG CAAGACGTTC GATACGTTCG ATCCCGCGAC GGGCGAATTG CTCGCGCGCG TTCAGCAGGC GAGCGCCGAC GATGTCGACC GCGCGGTCGC ATCCGCGCGC GAAGGCCAGC GCGAATGGGC CGCGATGACC GCGATGCAGC GCTCGCGCAT CCTGCGTCGC GCGGTCGAAT TGCTGCGCGA GCGCAACGAC GCGCTCGCCG AGCTCGAGAT GCGCGACACG GGCAAGCCGA TCGCGGAAAC GCGCGCCGTC GACATCGTCA CGGGCGCCGA CGTGATCGAG TACTACGCGG GCCTCGCGAC CGCGATCGAA GGTCTGCAGG TGCCGCTGCG CCCGGAATCG TTCGTCTACA CGCGGCGCGA GCCGCTCGGC GTGTGCGCGG GCATCGGCGC ATGGAACTAC CCGATCCAGA TCGCCTGCTG GAAGAGCGCG CCCGCGCTCG CCGCCGGCAA CGCGATGATC TTCAAGCCGA GCGAAGTCAC GCCGCTGTCC GCGCTCAAGC TCGCGGAGAT CTACACCGAG GCGGGAGTGC CCGCCGGCGT GTTCAACGTC GTGCAGGGCG ACGGCTCGGT CGGCGCGCTG CTCAGCGCGC ACCCGGGCAT CGCGAAGGTA TCGTTTACGG GCGGCGTCGA AACCGGCAAG AAGGTGATGT CGCTCGCGGG CGCATCGTCG CTGAAGGAAG TGACGATGGA ACTGGGCGGC AAATCGCCGC TCATCGTGTT CGACGACGCC GATCTCGACC GCGCGGCCGA CATCGCCGTC ACCGCGAACT TCTTCAGCGC GGGCCAGGTG TGCACGAACG GCACGCGCGT GTTCGTCCAG CAAGCGGTGA AGGACGCGTT CGTCGAGCGC GTGCTCGCGC GCGTCGCACG GATTCGGGTC GGCAAACCTT CGGATTCCGA CACGAATTTC GGACCGCTCG CGAGCGCCGC CCAGCTCGAC AAGGTGCTCG GCTACATCGA CAGCGGCAAG GCCGAAGGCG CGAAGCTGCT CGCGGGCGGC GCGCGCCTCG TGAACGATCA CTTCGCGAGC GGCCAGTACG TTGCGCCGAC CGTGTTCGGC GATTGCCGCG ACGACATGAG GATCGTCCGC GAAGAGATTT TCGGGCCGGT GATGAGCATC CTGTCGTTCG AAACGGAAGA CGAAGCGATC GCGCGCGCGA ACGCGACCGA CTACGGCCTC GCGGCGGGCG TCGTCACCGA GAACCTGTCG CGCGCGCACC GCGCGATCCA TCGCCTCGAA GCCGGCATCT GCTGGATCAA CACGTGGGGC GAATCGCCCG CCGAGATGCC GGTTGGCGGG TATAAGCAAT CCGGCGTCGG ACGCGAGAAC GGCATCACGA CGCTCGAGCA CTACACTCGA ATCAAATCGG TGCAGGTCAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA