BupsA.00014.a
isocitrate lyase
CENTER ID:
BupsA.00014.a
ORGANISM:
Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE:
Melioidosis
CURRENT STATUS:
in PDB
COMMUNITY REQUEST:
True
NIH RISK GROUP:
3
SELECT AGENT:
True
NIH PRIORITY
pathogens category:
IIIB
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO
AA
START
AA
STOP
ORDER
MATERIAL
BupsA.00014.a.A1.GE24990
Full length( BupsA.00014.a )
info
1
435
BupsA.00014.a.A1.GE24990
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis . Get sequence information
using the button in the "info" column.
Proteins
CENTER REFERENCE ID
DOMAIN/REGION DESCRIPTION
INFO
AA START
AA STOP
ORDER MATERIAL
BupsA.00014.a.D19.PD00020
Full length( BupsA.00014.a )
info
1
435
PD00020
BupsA.00014.a.A1.PW26982
Full length( BupsA.00014.a )
info
1
435
PW26982
Structures
3I4E
DEPOSITED: 7/1/2009
DETERMINATION: XRay
CLONE: BupsA.00014.a.A1.GE24990
PROTEIN: BupsA.00014.a.A1.PW25028
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSRQQQAQEL QKQWETDPRW KGIKRAFTAE DVVRLRGSIQ QEHTLAKRGA EKLWTLINNE PFVNALGALT GNQAMQQVKA GLKAIYLSGW QVAGDANVAG EMYPDQSLYP ANSVPLVVKR INNTLTRADQ IQWSEGKNPG DEGYVDFFAP IVADAEAGFG GVLNAFELMK AMIEAGASGV HFEDQLASVK KCGHMGGKVL VPTREAVAKL TAARLAADVM GTPTVLVART DAEAADLITS DIDDNDKPYL TGERTVEGFF RTKPGLEQAI SRGLAYAPYA DLIWCETGKP DLEYAKKFAE AIHKQFPGKL LSYNCSPSFN WKKNLDDATI AKFQKELGAM GYKFQFITLA GFHALNYSMF NLAHGYARTQ MSAFVELQQA EFAAADKGFT AVKHQREVGT GYFDAVTQTV EREASTTALH GSTEDEQFFD GQKVA
NT Sequence
ATGTCGCGTC AACAACAGGC TCAGGAACTG CAAAAGCAAT GGGAAACCGA CCCGCGCTGG AAGGGCATCA AGCGCGCGTT CACCGCTGAG GACGTCGTGC GCCTGCGCGG CTCGATCCAG CAAGAGCACA CGCTCGCGAA GCGCGGCGCG GAAAAGCTCT GGACGCTCAT CAACAACGAG CCGTTCGTCA ACGCACTCGG CGCGCTGACG GGCAACCAGG CGATGCAGCA GGTCAAGGCC GGCCTCAAGG CGATCTATCT GTCGGGCTGG CAAGTCGCGG GCGACGCGAA CGTCGCGGGC GAAATGTATC CGGACCAGTC GCTGTACCCG GCGAACTCGG TGCCGCTCGT CGTCAAGCGC ATCAACAACA CGCTCACGCG CGCCGATCAG ATCCAGTGGT CGGAAGGCAA GAACCCCGGC GACGAAGGCT ATGTCGACTT CTTCGCGCCG ATCGTCGCCG ACGCGGAAGC CGGCTTCGGC GGCGTGCTGA ACGCGTTCGA ACTGATGAAG GCGATGATCG AGGCGGGCGC GTCGGGCGTT CACTTCGAAG ACCAGCTCGC CTCGGTCAAG AAGTGCGGCC ACATGGGCGG CAAGGTGCTC GTGCCGACCC GCGAGGCGGT CGCGAAGCTG ACGGCCGCGC GTCTCGCCGC CGACGTGATG GGCACGCCGA CCGTGCTGGT CGCGCGCACC GACGCCGAAG CGGCCGACCT GATCACGTCC GACATCGACG ACAACGACAA GCCGTACCTG ACGGGCGAGC GCACGGTCGA AGGCTTCTTC CGCACGAAGC CGGGCCTCGA GCAGGCTATC TCGCGCGGCC TCGCCTATGC GCCGTACGCC GACCTGATCT GGTGCGAAAC CGGCAAGCCG GATCTCGAAT ACGCGAAGAA GTTCGCGGAA GCGATCCACA AGCAGTTCCC GGGCAAGCTG CTGTCGTACA ACTGCTCGCC GTCGTTCAAC TGGAAGAAGA ACCTCGACGA CGCGACGATC GCGAAGTTCC AGAAGGAACT CGGCGCGATG GGCTACAAGT TCCAGTTCAT CACGCTCGCC GGCTTCCATG CGCTGAACTA CTCGATGTTC AACCTCGCGC ACGGCTACGC CCGCACGCAG ATGAGCGCGT TCGTCGAACT GCAGCAAGCC GAGTTCGCGG CGGCCGACAA GGGCTTCACC GCGGTCAAGC ACCAGCGTGA AGTCGGCACC GGCTACTTCG ACGCGGTGAC GCAGACGGTC GAGCGCGAAG CGTCGACGAC CGCGCTGCAC GGCTCGACCG AAGACGAACA GTTCTTCGAC GGCCAGAAAG TCGCC
Details for BupsA.00014.a.A1.GE24990
HARVESTED ON:
3/31/2008
SEQUENCED ON:
6/16/2009
EXPECTED MW:
50kDa
OBSERVED MW:
50kDa
ANTIBIOTIC MARKER:
ampicillin
COUNT OF EXPRESSION COLONIES:
Too many to count (100+)
TOTAL EXPRESSION LEVEL:
High Expression
SOLUBLE EXPRESSION LEVEL
High Expression
EXPRESSION HOST:
data unavailable
SEQUENCING RESULT:
pass with incomplete coverage
PERCENT IDENTITY:
100
PERCENT COVERAGE:
100
Details for BupsA.00014.a.D19.PD00020
PURIFICATION DATe:
6/11/2008
CONCENTRATION:
27mg/ml
OBSERVED MW:
data unavailable
EXPRESSION LEVEL:
Moderate Expression
PROTEIN PURIFICATION BUFFER:
20mM Tris-HCl, pH 8.0, 200mM NaCl, 1% glycerol, 1mM TCEP
EXPRESSION HOST:
data unavailable
VIAL COUNT (approx.):
50
VIAL VOLUME:
50µl
PERCENT IDENTITY:
data unavailable
PERCENT COVERAGE:
data unavailable
Details for BupsA.00014.a.A1.PW26982
PURIFICATION DATe:
6/1/2009
CONCENTRATION:
25.89mg/ml
OBSERVED MW:
data unavailable
EXPRESSION LEVEL:
Moderate Expression
PROTEIN PURIFICATION BUFFER:
25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:
data unavailable
VIAL COUNT (approx.):
8
VIAL VOLUME:
100µl
PERCENT IDENTITY:
100
PERCENT COVERAGE:
100