BupsA.00014.a

isocitrate lyase

CENTER ID: BupsA.00014.a
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: in PDB
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:
IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
BupsA.00014.a.A1.GE24990 Full length( BupsA.00014.a ) 1 435
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
BupsA.00014.a.D19.PD00020 Full length( BupsA.00014.a ) 1 435
BupsA.00014.a.A1.PW26982 Full length( BupsA.00014.a ) 1 435

Structures

3I4E
DEPOSITED: 7/1/2009
DETERMINATION: XRay
CLONE: BupsA.00014.a.A1.GE24990
PROTEIN: BupsA.00014.a.A1.PW25028

External Resources

RESOURCE REFERENCE ID
OrthoMCL: OG5_129572
PATRIC ID: fig|320372.6.peg.2741
RefSeq: YP_334005
UniProt: Q3JQZ8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSRQQQAQEL QKQWETDPRW KGIKRAFTAE DVVRLRGSIQ QEHTLAKRGA EKLWTLINNE PFVNALGALT GNQAMQQVKA GLKAIYLSGW QVAGDANVAG EMYPDQSLYP ANSVPLVVKR INNTLTRADQ IQWSEGKNPG DEGYVDFFAP IVADAEAGFG GVLNAFELMK AMIEAGASGV HFEDQLASVK KCGHMGGKVL VPTREAVAKL TAARLAADVM GTPTVLVART DAEAADLITS DIDDNDKPYL TGERTVEGFF RTKPGLEQAI SRGLAYAPYA DLIWCETGKP DLEYAKKFAE AIHKQFPGKL LSYNCSPSFN WKKNLDDATI AKFQKELGAM GYKFQFITLA GFHALNYSMF NLAHGYARTQ MSAFVELQQA EFAAADKGFT AVKHQREVGT GYFDAVTQTV EREASTTALH GSTEDEQFFD GQKVA
NT Sequence
ATGTCGCGTC AACAACAGGC TCAGGAACTG CAAAAGCAAT GGGAAACCGA CCCGCGCTGG AAGGGCATCA AGCGCGCGTT CACCGCTGAG GACGTCGTGC GCCTGCGCGG CTCGATCCAG CAAGAGCACA CGCTCGCGAA GCGCGGCGCG GAAAAGCTCT GGACGCTCAT CAACAACGAG CCGTTCGTCA ACGCACTCGG CGCGCTGACG GGCAACCAGG CGATGCAGCA GGTCAAGGCC GGCCTCAAGG CGATCTATCT GTCGGGCTGG CAAGTCGCGG GCGACGCGAA CGTCGCGGGC GAAATGTATC CGGACCAGTC GCTGTACCCG GCGAACTCGG TGCCGCTCGT CGTCAAGCGC ATCAACAACA CGCTCACGCG CGCCGATCAG ATCCAGTGGT CGGAAGGCAA GAACCCCGGC GACGAAGGCT ATGTCGACTT CTTCGCGCCG ATCGTCGCCG ACGCGGAAGC CGGCTTCGGC GGCGTGCTGA ACGCGTTCGA ACTGATGAAG GCGATGATCG AGGCGGGCGC GTCGGGCGTT CACTTCGAAG ACCAGCTCGC CTCGGTCAAG AAGTGCGGCC ACATGGGCGG CAAGGTGCTC GTGCCGACCC GCGAGGCGGT CGCGAAGCTG ACGGCCGCGC GTCTCGCCGC CGACGTGATG GGCACGCCGA CCGTGCTGGT CGCGCGCACC GACGCCGAAG CGGCCGACCT GATCACGTCC GACATCGACG ACAACGACAA GCCGTACCTG ACGGGCGAGC GCACGGTCGA AGGCTTCTTC CGCACGAAGC CGGGCCTCGA GCAGGCTATC TCGCGCGGCC TCGCCTATGC GCCGTACGCC GACCTGATCT GGTGCGAAAC CGGCAAGCCG GATCTCGAAT ACGCGAAGAA GTTCGCGGAA GCGATCCACA AGCAGTTCCC GGGCAAGCTG CTGTCGTACA ACTGCTCGCC GTCGTTCAAC TGGAAGAAGA ACCTCGACGA CGCGACGATC GCGAAGTTCC AGAAGGAACT CGGCGCGATG GGCTACAAGT TCCAGTTCAT CACGCTCGCC GGCTTCCATG CGCTGAACTA CTCGATGTTC AACCTCGCGC ACGGCTACGC CCGCACGCAG ATGAGCGCGT TCGTCGAACT GCAGCAAGCC GAGTTCGCGG CGGCCGACAA GGGCTTCACC GCGGTCAAGC ACCAGCGTGA AGTCGGCACC GGCTACTTCG ACGCGGTGAC GCAGACGGTC GAGCGCGAAG CGTCGACGAC CGCGCTGCAC GGCTCGACCG AAGACGAACA GTTCTTCGAC GGCCAGAAAG TCGCC
Details for BupsA.00014.a.A1.GE24990
HARVESTED ON: 3/31/2008
SEQUENCED ON: 6/16/2009
EXPECTED MW: 50kDa
OBSERVED MW: 50kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSRQQQAQE LQKQWETDPR WKGIKRAFTA EDVVRLRGSI QQEHTLAKRG AEKLWTLINN EPFVNALGAL TGNQAMQQVK AGLKAIYLSG WQVAGDANVA GEMYPDQSLY PANSVPLVVK RINNTLTRAD QIQWSEGKNP GDEGYVDFFA PIVADAEAGF GGVLNAFELM KAMIEAGASG VHFEDQLASV KKCGHMGGKV LVPTREAVAK LTAARLAADV MGTPTVLVAR TDAEAADLIT SDIDDNDKPY LTGERTVEGF FRTKPGLEQA ISRGLAYAPY ADLIWCETGK PDLEYAKKFA EAIHKQFPGK LLSYNCSPSF NWKKNLDDAT IAKFQKELGA MGYKFQFITL AGFHALNYSM FNLAHGYART QMSAFVELQQ AEFAAADKGF TAVKHQREVG TGYFDAVTQT VEREASTTAL HGSTEDEQFF DGQKVA
Validated NT Sequence
ttaagaagga gatataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatgtcgcgt caacaacagg ctcaggaact gcaaaagcaa tgggaaaccg acccgcgctg gaagggcatc aagcgcgcgt tcaccgctga ggacgtcgtg cgcctgcgcg gctcgatcca gcaagagcac acgctcgcga agcgcggcgc ggaaaagctc tggacgctca tcaacaacga gccgttcgtc aacgcactcg gcgcgctgac gggcaaccag gcgatgcagc aggtcaaggc cggcctcaag gcgatctatc tgtcgggctg gcaagtcgcg ggcgacgcga acgtcgcggg cgaaatgtat ccggaccagt cgctgtaccc ggcgaactcg gtgccgctcg tcgtcaagcg catcaacaac acgctcacgc gcgccgatca gatccagtgg tcggaaggca agaaccccgg cgacgaaggc tatgtcgact tcttcgcgcc gatcgtcgcc gacgcggaag ccggcttcgg cggcgtgctg aacgcgttcg aactgatgaa ggcgatgatc gaggcgggcg cgtcgggcgt tcacttcgaa gaccagctcg cctcggtcaa gaagtgcggc cacatgggcg gcaaggtgct cgtgccgacc cgcgaggcgg tcgcgaagct gacggccgcg cgtctcgccg ccgacgtgat gggcacgccg accgtgctgg tcgcgcgcac cgacgccgaa gcggccgacc tgatcacgtc cgacatcgac gacaacgaca agccgtacct gacgggcgag cgcacggtcg aaggcttctt ccgcacgaag ccgggcctcg agcaggctat ctcgcgcggc ctcgcctatg cgccgtacgc cgacctgatc tggtgcgaaa ccggcaagcc ggatctcgaa tacgcgaaga agttcgcgga agcgatccac aagcagttcc cgggcaagct gctgtcgtac aactgctcgc cgtcgttcaa ctggaagaag aacctcgacg acgcgacgat cgcgaagttc cagaaggaac tcggcgcgat gggctacaag ttccagttca tcacgctcgc cggcttccat gcgctgaact actcgatgtt caacctcgcg cacggctacg cccgcacgca gatgagcgcg ttcgtcgaac tgcagcaagc cgagttcgcg gcggccgaca agggcttcac cgcggtcaag caccagcgtg aagtcggcac cggctacttc gacgcggtga cgcagacggt cgagcgcgaa gcgtcgacga ccgcgctgca cggctcgacc gaagacgaac agttcttcga cggccagaaa gtcgcctaa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSRQQQAQE LQKQWETDPR WKGIKRAFTA EDVVRLRGSI QQEHTLAKRG AEKLWTLINN EPFVNALGAL TGNQAMQQVK AGLKAIYLSG WQVAGDANVA GEMYPDQSLY PANSVPLVVK RINNTLTRAD QIQWSEGKNP GDEGYVDFFA PIVADAEAGF GGVLNAFELM KAMIEAGASG VHFEDQLASV KKCGHMGGKV LVPTREAVAK LTAARLAADV MGTPTVLVAR TDAEAADLIT SDIDDNDKPY LTGERTVEGF FRTKPGLEQA ISRGLAYAPY ADLIWCETGK PDLEYAKKFA EAIHKQFPGK LLSYNCSPSF NWKKNLDDAT IAKFQKELGA MGYKFQFITL AGFHALNYSM FNLAHGYART QMSAFVELQQ AEFAAADKGF TAVKHQREVG TGYFDAVTQT VEREASTTAL HGSTEDEQFF DGQKVA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcgcgtcaa caacaggctc aggaactgca aaagcaatgg gaaaccgacc cgcgctggaa gggcatcaag cgcgcgttca ccgctgagga cgtcgtgcgc ctgcgcggct cgatccagca agagcacacg ctcgcgaagc gcggcgcgga aaagctctgg acgctcatca acaacgagcc gttcgtcaac gcactcggcg cgctgacggg caaccaggcg atgcagcagg tcaaggccgg cctcaaggcg atctatctgt cgggctggca agtcgcgggc gacgcgaacg tcgcgggcga aatgtatccg gaccagtcgc tgtacccggc gaactcggtg ccgctcgtcg tcaagcgcat caacaacacg ctcacgcgcg ccgatcagat ccagtggtcg gaaggcaaga accccggcga cgaaggctat gtcgacttct tcgcgccgat cgtcgccgac gcggaagccg gcttcggcgg cgtgctgaac gcgttcgaac tgatgaaggc gatgatcgag gcgggcgcgt cgggcgttca cttcgaagac cagctcgcct cggtcaagaa gtgcggccac atgggcggca aggtgctcgt gccgacccgc gaggcggtcg cgaagctgac ggccgcgcgt ctcgccgccg acgtgatggg cacgccgacc gtgctggtcg cgcgcaccga cgccgaagcg gccgacctga tcacgtccga catcgacgac aacgacaagc cgtacctgac gggcgagcgc acggtcgaag gcttcttccg cacgaagccg ggcctcgagc aggctatctc gcgcggcctc gcctatgcgc cgtacgccga cctgatctgg tgcgaaaccg gcaagccgga tctcgaatac gcgaagaagt tcgcggaagc gatccacaag cagttcccgg gcaagctgct gtcgtacaac tgctcgccgt cgttcaactg gaagaagaac ctcgacgacg cgacgatcgc gaagttccag aaggaactcg gcgcgatggg ctacaagttc cagttcatca cgctcgccgg cttccatgcg ctgaactact cgatgttcaa cctcgcgcac ggctacgccc gcacgcagat gagcgcgttc gtcgaactgc agcaagccga gttcgcggcg gccgacaagg gcttcaccgc ggtcaagcac cagcgtgaag tcggcaccgg ctacttcgac gcggtgacgc agacggtcga gcgcgaagcg tcgacgaccg cgctgcacgg ctcgaccgaa gacgaacagt tcttcgacgg ccagaaagtc gcctgaaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for BupsA.00014.a.D19.PD00020
PURIFICATION DATe: 6/11/2008
CONCENTRATION: 27mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20mM Tris-HCl, pH 8.0, 200mM NaCl, 1% glycerol, 1mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 50
VIAL VOLUME: 50µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGSMSR QQQAQELQKQ WETDPRWKGI KRAFTAEDVV RLRGSIQQEH TLAKRGAEKL WTLINNEPFV NALGALTGNQ AMQQVKAGLK AIYLSGWQVA GDANVAGEMY PDQSLYPANS VPLVVKRINN TLTRADQIQW SEGKNPGDEG YVDFFAPIVA DAEAGFGGVL NAFELMKAMI EAGASGVHFE DQLASVKKCG HMGGKVLVPT REAVAKLTAA RLAADVMGTP TVLVARTDAE AADLITSDID DNDKPYLTGE RTVEGFFRTK PGLEQAISRG LAYAPYADLI WCETGKPDLE YAKKFAEAIH KQFPGKLLSY NCSPSFNWKK NLDDATIAKF QKELGAMGYK FQFITLAGFH ALNYSMFNLA HGYARTQMSA FVELQQAEFA AADKGFTAVK HQREVGTGYF DAVTQTVERE ASTTALHGST EDEQFFDGQK VA
Full NT Sequence (Expression Vector + Insert)
TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AATTAATTCT TAGAAAAACT CATCGAGCAT CAAATGAAAC TGCAATTTAT TCATATCAGG ATTATCAATA CCATATTTTT GAAAAAGCCG TTTCTGTAAT GAAGGAGAAA ACTCACCGAG GCAGTTCCAT AGGATGGCAA GATCCTGGTA TCGGTCTGCG ATTCCGACTC GTCCAACATC AATACAACCT ATTAATTTCC CCTCGTCAAA AATAAGGTTA TCAAGTGAGA AATCACCATG AGTGACGACT GAATCCGGTG AGAATGGCAA AAGTTTATGC ATTTCTTTCC AGACTTGTTC AACAGGCCAG CCATTACGCT CGTCATCAAA ATCACTCGCA TCAACCAAAC CGTTATTCAT TCGTGATTGC GCCTGAGCGA GACGAAATAC GCGATCGCTG TTAAAAGGAC AATTACAAAC AGGAATCGAA TGCAACCGGC GCAGGAACAC TGCCAGCGCA TCAACAATAT TTTCACCTGA ATCAGGATAT TCTTCTAATA CCTGGAATGC TGTTTTCCCG GGGATCGCAG TGGTGAGTAA CCATGCATCA TCAGGAGTAC GGATAAAATG CTTGATGGTC GGAAGAGGCA TAAATTCCGT CAGCCAGTTT AGTCTGACCA TCTCATCTGT AACATCATTG GCAACGCTAC CTTTGCCATG TTTCAGAAAC AACTCTGGCG CATCGGGCTT CCCATACAAT CGATAGATTG TCGCACCTGA TTGCCCGACA TTATCGCGAG CCCATTTATA CCCATATAAA TCAGCATCCA TGTTGGAATT TAATCGCGGC CTAGAGCAAG ACGTTTCCCG TTGAATATGG CTCATAACAC CCCTTGTATT ACTGTTTATG TAAGCAGACA GTTTTATTGT TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGAGATC CCGGTGCCTA ATGAGTGAGC TAACTTACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCCAG GGTGGTTTTT CTTTTCACCA GTGAGACGGG CAACAGCTGA TTGCCCTTCA CCGCCTGGCC CTGAGAGAGT TGCAGCAAGC GGTCCACGCT GGTTTGCCCC AGCAGGCGAA AATCCTGTTT GATGGTGGTT AACGGCGGGA TATAACATGA GCTGTCTTCG GTATCGTCGT ATCCCACTAC CGAGATATCC GCACCAACGC GCAGCCCGGA CTCGGTAATG GCGCGCATTG CGCCCAGCGC CATCTGATCG TTGGCAACCA GCATCGCAGT GGGAACGATG CCCTCATTCA GCATTTGCAT GGTTTGTTGA AAACCGGACA TGGCACTCCA GTCGCCTTCC CGTTCCGCTA TCGGCTGAAT TTGATTGCGA GTGAGATATT TATGCCAGCC AGCCAGACGC AGACGCGCCG AGACAGAACT TAATGGGCCC GCTAACAGCG CGATTTGCTG GTGACCCAAT GCGACCAGAT GCTCCACGCC CAGTCGCGTA CCGTCTTCAT GGGAGAAAAT AATACTGTTG ATGGGTGTCT GGTCAGAGAC ATCAAGAAAT AACGCCGGAA CATTAGTGCA GGCAGCTTCC ACAGCAATGG CATCCTGGTC ATCCAGCGGA TAGTTAATGA TCAGCCCACT GACGCGTTGC GCGAGAAGAT TGTGCACCGC CGCTTTACAG GCTTCGACGC CGCTTCGTTC TACCATCGAC ACCACCACGC TGGCACCCAG TTGATCGGCG CGAGATTTAA TCGCCGCGAC AATTTGCGAC GGCGCGTGCA GGGCCAGACT GGAGGTGGCA ACGCCAATCA GCAACGACTG TTTGCCCGCC AGTTGTTGTG CCACGCGGTT GGGAATGTAA TTCAGCTCCG CCATCGCCGC TTCCACTTTT TCCCGCGTTT TCGCAGAAAC GTGGCTGGCC TGGTTCACCA CGCGGGAAAC GGTCTGATAA GAGACACCGG CATACTCTGC GACATCGTAT AACGTTACTG GTTTCACATT CACCACCCTG AATTGACTCT CTTCCGGGCG CTATCATGCC ATACCGCGAA AGGTTTTGCG CCATTCGATG GTGTCCGGGA TCTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AATTAATACG ACTCACTATA GGGGAATTGT GAGCGGATAA CAATTCCCCT CTAGAAATAA TTTTGTTTAA CTTTAAGAAG GAGATATACC ATGGGCCACC ACCACCACCA CCATAGCGGC GAAGTAAAAC CGGAAGTGAA GCCGGAGACC CACATCAACC TGAAGGTTAG CGACGGTAGC AGCGAAATCT TCTTCAAGAT TAAGAAGACC ACCCCGCTGC GTCGCCTGAT GGAAGCATTC GCTAAACGCC AGGGCAAGGA AATGGATTCC CTGCGCTTTC TGTACGACGG TATCCGTATT CAGGCAGACC AGACTCCGGA AGATCTGGAC ATGGAAGATA ACGACATTAT CGAAGCACAC CGTGAACAAA TCGGTGGATC CATGTCGCGT CAACAACAGG CTCAGGAACT GCAAAAGCAA TGGGAAACCG ACCCGCGCTG GAAGGGCATC AAGCGCGCGT TCACCGCTGA GGACGTCGTG CGCCTGCGCG GCTCGATCCA GCAAGAGCAC ACGCTCGCGA AGCGCGGCGC GGAAAAGCTC TGGACGCTCA TCAACAACGA GCCGTTCGTC AACGCACTCG GCGCGCTGAC GGGCAACCAG GCGATGCAGC AGGTCAAGGC CGGCCTCAAG GCGATCTATC TGTCGGGCTG GCAAGTCGCG GGCGACGCGA ACGTCGCGGG CGAAATGTAT CCGGACCAGT CGCTGTACCC GGCGAACTCG GTGCCGCTCG TCGTCAAGCG CATCAACAAC ACGCTCACGC GCGCCGATCA GATCCAGTGG TCGGAAGGCA AGAACCCCGG CGACGAAGGC TATGTCGACT TCTTCGCGCC GATCGTCGCC GACGCGGAAG CCGGCTTCGG CGGCGTGCTG AACGCGTTCG AACTGATGAA GGCGATGATC GAGGCGGGCG CGTCGGGCGT TCACTTCGAA GACCAGCTCG CCTCGGTCAA GAAGTGCGGC CACATGGGCG GCAAGGTGCT CGTGCCGACC CGCGAGGCGG TCGCGAAGCT GACGGCCGCG CGTCTCGCCG CCGACGTGAT GGGCACGCCG ACCGTGCTGG TCGCGCGCAC CGACGCCGAA GCGGCCGACC TGATCACGTC CGACATCGAC GACAACGACA AGCCGTACCT GACGGGCGAG CGCACGGTCG AAGGCTTCTT CCGCACGAAG CCGGGCCTCG AGCAGGCTAT CTCGCGCGGC CTCGCCTATG CGCCGTACGC CGACCTGATC TGGTGCGAAA CCGGCAAGCC GGATCTCGAA TACGCGAAGA AGTTCGCGGA AGCGATCCAC AAGCAGTTCC CGGGCAAGCT GCTGTCGTAC AACTGCTCGC CGTCGTTCAA CTGGAAGAAG AACCTCGACG ACGCGACGAT CGCGAAGTTC CAGAAGGAAC TCGGCGCGAT GGGCTACAAG TTCCAGTTCA TCACGCTCGC CGGCTTCCAT GCGCTGAACT ACTCGATGTT CAACCTCGCG CACGGCTACG CCCGCACGCA GATGAGCGCG TTCGTCGAAC TGCAGCAAGC CGAGTTCGCG GCGGCCGACA AGGGCTTCAC CGCGGTCAAG CACCAGCGTG AAGTCGGCAC CGGCTACTTC GACGCGGTGA CGCAGACGGT CGAGCGCGAA GCGTCGACGA CCGCGCTGCA CGGCTCGACC GAAGACGAAC AGTTCTTCGA CGGCCAGAAA GTCGCCTGAT AAAAGCTTGC GGCCGCACTC GAGCACCACC ACCACCACCA CTGAGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT
Details for BupsA.00014.a.A1.PW26982
PURIFICATION DATe: 6/1/2009
CONCENTRATION: 25.89mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 8
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSRQQQAQE LQKQWETDPR WKGIKRAFTA EDVVRLRGSI QQEHTLAKRG AEKLWTLINN EPFVNALGAL TGNQAMQQVK AGLKAIYLSG WQVAGDANVA GEMYPDQSLY PANSVPLVVK RINNTLTRAD QIQWSEGKNP GDEGYVDFFA PIVADAEAGF GGVLNAFELM KAMIEAGASG VHFEDQLASV KKCGHMGGKV LVPTREAVAK LTAARLAADV MGTPTVLVAR TDAEAADLIT SDIDDNDKPY LTGERTVEGF FRTKPGLEQA ISRGLAYAPY ADLIWCETGK PDLEYAKKFA EAIHKQFPGK LLSYNCSPSF NWKKNLDDAT IAKFQKELGA MGYKFQFITL AGFHALNYSM FNLAHGYART QMSAFVELQQ AEFAAADKGF TAVKHQREVG TGYFDAVTQT VEREASTTAL HGSTEDEQFF DGQKVA
Validated NT Sequence
ttaagaagga gatataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatgtcgcgt caacaacagg ctcaggaact gcaaaagcaa tgggaaaccg acccgcgctg gaagggcatc aagcgcgcgt tcaccgctga ggacgtcgtg cgcctgcgcg gctcgatcca gcaagagcac acgctcgcga agcgcggcgc ggaaaagctc tggacgctca tcaacaacga gccgttcgtc aacgcactcg gcgcgctgac gggcaaccag gcgatgcagc aggtcaaggc cggcctcaag gcgatctatc tgtcgggctg gcaagtcgcg ggcgacgcga acgtcgcggg cgaaatgtat ccggaccagt cgctgtaccc ggcgaactcg gtgccgctcg tcgtcaagcg catcaacaac acgctcacgc gcgccgatca gatccagtgg tcggaaggca agaaccccgg cgacgaaggc tatgtcgact tcttcgcgcc gatcgtcgcc gacgcggaag ccggcttcgg cggcgtgctg aacgcgttcg aactgatgaa ggcgatgatc gaggcgggcg cgtcgggcgt tcacttcgaa gaccagctcg cctcggtcaa gaagtgcggc cacatgggcg gcaaggtgct cgtgccgacc cgcgaggcgg tcgcgaagct gacggccgcg cgtctcgccg ccgacgtgat gggcacgccg accgtgctgg tcgcgcgcac cgacgccgaa gcggccgacc tgatcacgtc cgacatcgac gacaacgaca agccgtacct gacgggcgag cgcacggtcg aaggcttctt ccgcacgaag ccgggcctcg agcaggctat ctcgcgcggc ctcgcctatg cgccgtacgc cgacctgatc tggtgcgaaa ccggcaagcc ggatctcgaa tacgcgaaga agttcgcgga agcgatccac aagcagttcc cgggcaagct gctgtcgtac aactgctcgc cgtcgttcaa ctggaagaag aacctcgacg acgcgacgat cgcgaagttc cagaaggaac tcggcgcgat gggctacaag ttccagttca tcacgctcgc cggcttccat gcgctgaact actcgatgtt caacctcgcg cacggctacg cccgcacgca gatgagcgcg ttcgtcgaac tgcagcaagc cgagttcgcg gcggccgaca agggcttcac cgcggtcaag caccagcgtg aagtcggcac cggctacttc gacgcggtga cgcagacggt cgagcgcgaa gcgtcgacga ccgcgctgca cggctcgacc gaagacgaac agttcttcga cggccagaaa gtcgcctaa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSRQQQAQE LQKQWETDPR WKGIKRAFTA EDVVRLRGSI QQEHTLAKRG AEKLWTLINN EPFVNALGAL TGNQAMQQVK AGLKAIYLSG WQVAGDANVA GEMYPDQSLY PANSVPLVVK RINNTLTRAD QIQWSEGKNP GDEGYVDFFA PIVADAEAGF GGVLNAFELM KAMIEAGASG VHFEDQLASV KKCGHMGGKV LVPTREAVAK LTAARLAADV MGTPTVLVAR TDAEAADLIT SDIDDNDKPY LTGERTVEGF FRTKPGLEQA ISRGLAYAPY ADLIWCETGK PDLEYAKKFA EAIHKQFPGK LLSYNCSPSF NWKKNLDDAT IAKFQKELGA MGYKFQFITL AGFHALNYSM FNLAHGYART QMSAFVELQQ AEFAAADKGF TAVKHQREVG TGYFDAVTQT VEREASTTAL HGSTEDEQFF DGQKVA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCGCGTCAA CAACAGGCTC AGGAACTGCA AAAGCAATGG GAAACCGACC CGCGCTGGAA GGGCATCAAG CGCGCGTTCA CCGCTGAGGA CGTCGTGCGC CTGCGCGGCT CGATCCAGCA AGAGCACACG CTCGCGAAGC GCGGCGCGGA AAAGCTCTGG ACGCTCATCA ACAACGAGCC GTTCGTCAAC GCACTCGGCG CGCTGACGGG CAACCAGGCG ATGCAGCAGG TCAAGGCCGG CCTCAAGGCG ATCTATCTGT CGGGCTGGCA AGTCGCGGGC GACGCGAACG TCGCGGGCGA AATGTATCCG GACCAGTCGC TGTACCCGGC GAACTCGGTG CCGCTCGTCG TCAAGCGCAT CAACAACACG CTCACGCGCG CCGATCAGAT CCAGTGGTCG GAAGGCAAGA ACCCCGGCGA CGAAGGCTAT GTCGACTTCT TCGCGCCGAT CGTCGCCGAC GCGGAAGCCG GCTTCGGCGG CGTGCTGAAC GCGTTCGAAC TGATGAAGGC GATGATCGAG GCGGGCGCGT CGGGCGTTCA CTTCGAAGAC CAGCTCGCCT CGGTCAAGAA GTGCGGCCAC ATGGGCGGCA AGGTGCTCGT GCCGACCCGC GAGGCGGTCG CGAAGCTGAC GGCCGCGCGT CTCGCCGCCG ACGTGATGGG CACGCCGACC GTGCTGGTCG CGCGCACCGA CGCCGAAGCG GCCGACCTGA TCACGTCCGA CATCGACGAC AACGACAAGC CGTACCTGAC GGGCGAGCGC ACGGTCGAAG GCTTCTTCCG CACGAAGCCG GGCCTCGAGC AGGCTATCTC GCGCGGCCTC GCCTATGCGC CGTACGCCGA CCTGATCTGG TGCGAAACCG GCAAGCCGGA TCTCGAATAC GCGAAGAAGT TCGCGGAAGC GATCCACAAG CAGTTCCCGG GCAAGCTGCT GTCGTACAAC TGCTCGCCGT CGTTCAACTG GAAGAAGAAC CTCGACGACG CGACGATCGC GAAGTTCCAG AAGGAACTCG GCGCGATGGG CTACAAGTTC CAGTTCATCA CGCTCGCCGG CTTCCATGCG CTGAACTACT CGATGTTCAA CCTCGCGCAC GGCTACGCCC GCACGCAGAT GAGCGCGTTC GTCGAACTGC AGCAAGCCGA GTTCGCGGCG GCCGACAAGG GCTTCACCGC GGTCAAGCAC CAGCGTGAAG TCGGCACCGG CTACTTCGAC GCGGTGACGC AGACGGTCGA GCGCGAAGCG TCGACGACCG CGCTGCACGG CTCGACCGAA GACGAACAGT TCTTCGACGG CCAGAAAGTC GCCTGAAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA