BupsA.00013.a

Holliday junction DNA helicase RuvB

CENTER ID: BupsA.00013.a
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.00013.a.D11.GE00001 Full length( BupsA.00013.a ) 1 356
BupsA.00013.a.D12.GE00001 Full length( BupsA.00013.a ) 1 340
BupsA.00013.a.D13.GE00001 Full length( BupsA.00013.a ) 24 356
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.00013.a.D11.PD00050 Full length( BupsA.00013.a ) 1 356
BupsA.00013.a.D11.PD00051 Full length( BupsA.00013.a ) 1 356
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_132500
PATRIC ID: fig|320372.6.peg.3546
RefSeq: YP_334778
UniProt: Q3JNS5
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MIETDKLAAE RIIAATPASS HEEAFERALR PRQLDEYVGQ EKVRDQLEIF IEAAKRRSEA LDHVLLFGPP GLGKTTLAHI IAREMGVNLR QTSGPVLERA GDLAALLTNL EANDVLFIDE IHRLSPVVEE ILYPALEDYQ IDIMIGEGPA ARSVKLDLQP FTLVGATTRA GMLTNPLRDR FGIVARLEFY DAEQLSRIVR RSAALLNAQI DPAGALEIAK RSRGTPRIAN RLLRRVRDYA EVKADGNITA AVADAALAML DVDPVGFDLM DRKLLEAILH KFDGGPVGVD NLAAAIGEER DTIEDVLEPY LIQQGFLQRT PRGRVATLLT YRHFGLSAPD AANPVRNLWD TPDAEC
NT Sequence
ATGATCGAAA CCGACAAACT CGCCGCCGAG CGGATCATTG CCGCCACGCC CGCGTCTTCG CACGAAGAGG CTTTCGAACG CGCGCTGCGC CCGCGCCAGC TCGACGAATA CGTCGGCCAG GAGAAGGTGC GCGATCAGCT CGAAATCTTC ATCGAAGCCG CGAAGCGCCG CTCCGAAGCG CTCGACCACG TGCTGCTGTT CGGCCCGCCC GGCCTCGGCA AGACGACGCT CGCGCACATC ATCGCGCGCG AAATGGGCGT GAACCTGCGC CAGACGTCCG GCCCCGTGCT CGAGCGCGCG GGCGACCTCG CCGCGCTGCT CACGAACCTC GAAGCGAACG ACGTCCTCTT CATCGACGAG ATCCACCGCC TGTCGCCCGT CGTCGAGGAA ATCCTGTATC CGGCGCTCGA GGATTACCAG ATCGACATCA TGATCGGCGA AGGGCCAGCC GCGCGCAGCG TGAAGCTCGA CCTGCAGCCG TTCACGCTCG TCGGCGCGAC GACGCGCGCG GGCATGCTGA CCAATCCGCT GCGCGACCGC TTCGGCATCG TCGCGCGGCT CGAGTTCTAC GACGCCGAGC AGCTATCGCG CATCGTGCGC CGCTCGGCCG CGCTGCTGAA CGCGCAGATC GATCCGGCGG GCGCGCTCGA GATCGCGAAG CGCTCGCGCG GCACGCCGCG GATCGCGAAC CGCCTGCTGC GCCGCGTGCG CGACTACGCG GAAGTGAAGG CGGACGGCAA CATCACCGCG GCCGTCGCGG ACGCCGCGCT CGCGATGCTC GACGTCGATC CGGTCGGCTT CGACCTGATG GACCGCAAGC TGCTCGAGGC GATCCTGCAC AAGTTCGACG GCGGGCCGGT CGGCGTCGAC AACCTCGCCG CCGCGATCGG CGAGGAGCGC GACACGATCG AGGACGTGCT CGAGCCGTAC CTGATTCAGC AGGGCTTCTT GCAGCGCACG CCGCGCGGCC GCGTCGCGAC GCTCCTCACG TACCGGCATT TCGGTCTCTC CGCGCCCGAC GCCGCGAACC CCGTGCGCAA TCTCTGGGAC ACGCCCGACG CCGAGTGC
Details for BupsA.00013.a.D11.GE00001
HARVESTED ON: data unavailable
SEQUENCED ON: 7/29/2009
EXPECTED MW: 0kDa
OBSERVED MW: data unavailable
ANTIBIOTIC MARKER: kanamycin
COUNT OF EXPRESSION COLONIES: data unavailable
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 14
PERCENT COVERAGE: 75
Validated AA Sequence
MAVGQQGGNT TTRSTLEETL LDQVRLQYVF DGVTFFANGS CQVIYTNWTA VELMQDSFQE LTIHQIEAYR VNIQHSQSCV SYGSSDVTVS LNFCVITHTT QQAVSDTRST AATLSDFEGA SRIDLSVQQG GRTANDTAQL FSVVEFQTSN DAETVTQRVG QHTCTGGSAH QGKRLQIQLD GASSRTFTNH DIDLVVLQGR VKDLFHDRRQ AVDLVNEQYV VSLEVGQQCS EIASAFKDRA GSLAXXHAXL ACDDMCXGGL AEARRAXQXN VXXGLGTTFG SFXXIXSWXX TXXXXTXSSS XXXXXXXXXX XXWXXXXXXX YXXXXXXXXX XXXXXXXXX
Validated NT Sequence
ttcnggcttt gttagcagcc ggntctcngt ggtggtggtg gtggtgctcg agtgcggccg caagctttta gcactcagcg tcaggagtat cccacaggtt gcgtaccggg ttagcagcat ctggtgcgga cagaccgaaa tggcggtagg tcagcagggt ggcaacacga ccacgaggag tacgctggag gaaaccttgc tggatcaggt aaggctccag tacgtcttcg atggtgtcac gttcttcgcc aatggcagct gccaggttat ctacaccaac tggaccgccg tcgaacttat gcaggatagc ttccaggagc ttacgatcca tcagatcgaa gcctacaggg tcaacatcca gcatagccag agctgcgtca gctacggcag cagtgatgtt accgtcagcc ttaacttctg cgtaatcacg cacacgacgc agcaggcggt tagcgatacg aggagtaccg cggctacgct tagcgatttc gagggcgcca gccggatcga tctgagcgtt cagcagggcg gcagaacggc gaacgatacg gctcagttgt tcagcgtcgt agaattccag acgagcaacg atgccgaaac ggtcacgcag cgggttggtc agcatacctg cacgggtggt agcgcccacc agggtaaacg gctgcagatc cagcttgacg gagcgagcag caggaccttc accaatcatg atatcgatct ggtagtcctc cagggccggg taaaggatct cttccacgac aggagacagg cggtggatct cgtcaatgaa cagtacgtcg ttagcctcga ggttggtcag cagtgcagcg agatcgccag cgcgttcaag gacagggccg gaagtctggc gganantcac gccnatctcg cgtgcgatga tatgtgccnn ggnggtcttg ccgaggccag gagggccnaa cagcanaacg tggncanggg cctcggaacg acgtttggna gcttcntgna natttnnagc tggncnngta cnnncnnntn gnnnacgtan tcgtcaagct nanncnnnnn nnnnncncnt nnnaanncnt nnnnatggnn nntannnnnn nnnnnnnnnt acntnnngna nnnnnnnnnn nannctnann nnnnnnnnnn nnnnnnnnnn nnnnnntaat nnnnnnnnnn nnnnnggnnn nnnncngnnn nnnnng
Expected Protein Sequence
MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGMIET DKLAAERIIA ATPASSHEEA FERALRPRQL DEYVGQEKVR DQLEIFIEAA KRRSEALDHV LLFGPPGLGK TTLAHIIARE MGVNLRQTSG PVLERAGDLA ALLTNLEAND VLFIDEIHRL SPVVEEILYP ALEDYQIDIM IGEGPAARSV KLDLQPFTLV GATTRAGMLT NPLRDRFGIV ARLEFYDAEQ LSRIVRRSAA LLNAQIDPAG ALEIAKRSRG TPRIANRLLR RVRDYAEVKA DGNITAAVAD AALAMLDVDP VGFDLMDRKL LEAILHKFDG GPVGVDNLAA AIGEERDTIE DVLEPYLIQQ GFLQRTPRGR VATLLTYRHF GLSAPDAANP VRNLWDTPDA EC
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag gagatatacc atgggccacc accaccacca ccatagcggc gaagtaaaac cggaagtgaa gccggagacc cacatcaacc tgaaggttag cgacggtagc agcgaaatct tcttcaagat taagaagacc accccgctgc gtcgcctgat ggaagcattc gctaaacgcc agggcaagga aatggattcc ctgcgctttc tgtacgacgg tatccgtatt caggcagacc agactccgga agatctggac atggaagata acgacattat cgaagcacac cgtgaacaaa tcggtggaat gattgagact gacaagctgg ctgccgaacg tattatcgcc gccactcctg ctagcagcca tgaggaagca ttcgaacgtg ctctgcgtcc gcgtcagctt gacgaatacg taggccaaga gaaggtacgc gaccagctgg aaatcttcat tgaagctgcc aaacgtcgtt ccgaggccct tgaccacgtt ctgctgttcg gccctcctgg cctcggcaag accaccctgg cacatatcat cgcacgcgag atgggcgtga atctccgcca gacttccggc cctgtccttg aacgcgctgg cgatctcgct gcactgctga ccaacctcga ggctaacgac gtactgttca ttgacgagat ccaccgcctg tctcctgtcg tggaagagat cctttacccg gccctggagg actaccagat cgatatcatg attggtgaag gtcctgctgc tcgctccgtc aagctggatc tgcagccgtt taccctggtg ggcgctacca cccgtgcagg tatgctgacc aacccgctgc gtgaccgttt cggcatcgtt gctcgtctgg aattctacga cgctgaacaa ctgagccgta tcgttcgccg ttctgccgcc ctgctgaacg ctcagatcga tccggctggc gccctcgaaa tcgctaagcg tagccgcggt actcctcgta tcgctaaccg cctgctgcgt cgtgtgcgtg attacgcaga agttaaggct gacggtaaca tcactgctgc cgtagctgac gcagctctgg ctatgctgga tgttgaccct gtaggcttcg atctgatgga tcgtaagctc ctggaagcta tcctgcataa gttcgacggc ggtccagttg gtgtagataa cctggcagct gccattggcg aagaacgtga caccatcgaa gacgtactgg agccttacct gatccagcaa ggtttcctcc agcgtactcc tcgtggtcgt gttgccaccc tgctgaccta ccgccatttc ggtctgtccg caccagatgc tgctaacccg gtacgcaacc tgtgggatac tcctgacgct gagtgctaaa agcttgcggc cgcactcgag caccaccacc accaccactg agatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggat
Details for BupsA.00013.a.D12.GE00001
HARVESTED ON: data unavailable
SEQUENCED ON: 7/29/2009
EXPECTED MW: 0kDa
OBSERVED MW: data unavailable
ANTIBIOTIC MARKER: kanamycin
COUNT OF EXPRESSION COLONIES: data unavailable
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGMIET DKLAAERIIA ATPASSHEEA FERALRPRQL DEYVGQEKVR DQLEIFIEAA KRRSEALDHV LLFGPPGLGK TTLAHIIARE MGVNLRQTSG PVLERAGDLA ALLTNLEAND VLFIDEIHRL SPVVEEILYP ALEDYQIDIM IGEGPAARSV KLDLQPFTLV GATTRAGMLT NPLRDRFGIV ARLEFYDAEQ LSRIVRRSAA LLNAQIDPAG ALEIAKRSRG TPRIANRLLR RVRDYAEVKA DGNITAAVAD AALAMLDVDP VGFDLMDRKL LEAILHKFDG GPVGVDNLAA AIGEERDTIE DVLEPYLIQQ GFLQRTPRGR VATLLTYRHF GLSAPD
Validated NT Sequence
atgggccacc accaccacca ccatagcggc gaagtaaaac cggaagtgaa gccggagacc cacatcaacc tgaaggttag cgacggtagc agcgaaatct tcttcaagat taagaagacc accccgctgc gtcgcctgat ggaagcattc gctaaacgcc agggcaagga aatggattcc ctgcgctttc tgtacgacgg tatccgtatt caggcagacc agactccgga agatctggac atggaagata acgacattat cgaagcacac cgtgaacaaa tcggtggaat gattgagact gacaagctgg ctgccgaacg tattatcgcc gccactcctg ctagcagcca tgaggaagca ttcgaacgtg ctctgcgtcc gcgtcagctt gacgaatacg taggccaaga gaaggtacgc gaccagctgg aaatcttcat tgaagctgcc aaacgtcgtt ccgaggccct tgaccacgtt ctgctgttcg gccctcctgg cctcggcaag accaccctgg cacatatcat cgcacgcgag atgggcgtga atctccgcca gacttccggc cctgtccttg aacgcgctgg cgatctcgct gcactgctga ccaacctcga ggctaacgac gtactgttca ttgacgagat ccaccgcctg tctcctgtcg tggaagagat cctttacccg gccctggagg actaccagat cgatatcatg attggtgaag gtcctgctgc tcgctccgtc aagctggatc tgcagccgtt taccctggtg ggcgctacca cccgtgcagg tatgctgacc aacccgctgc gtgaccgttt cggcatcgtt gctcgtctgg aattctacga cgctgaacaa ctgagccgta tcgttcgccg ttctgccgcc ctgctgaacg ctcagatcga tccggctggc gccctcgaaa tcgctaagcg tagccgcggt actcctcgta tcgctaaccg cctgctgcgt cgtgtgcgtg attacgcaga agttaaggct gacggtaaca tcactgctgc cgtagctgac gcagctctgg ctatgctgga tgttgaccct gtaggcttcg atctgatgga tcgtaagctc ctggaagcta tcctgcataa gttcgacggc ggtccagttg gtgtagataa cctggcagct gccattggcg aagaacgtga caccatcgaa gacgtactgg agccttacct gatccagcaa ggtttcctcc agcgtactcc tcgtggtcgt gttgccaccc tgctgaccta ccgccatttc ggtctgtccg caccagatta a
Expected Protein Sequence
MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGMIET DKLAAERIIA ATPASSHEEA FERALRPRQL DEYVGQEKVR DQLEIFIEAA KRRSEALDHV LLFGPPGLGK TTLAHIIARE MGVNLRQTSG PVLERAGDLA ALLTNLEAND VLFIDEIHRL SPVVEEILYP ALEDYQIDIM IGEGPAARSV KLDLQPFTLV GATTRAGMLT NPLRDRFGIV ARLEFYDAEQ LSRIVRRSAA LLNAQIDPAG ALEIAKRSRG TPRIANRLLR RVRDYAEVKA DGNITAAVAD AALAMLDVDP VGFDLMDRKL LEAILHKFDG GPVGVDNLAA AIGEERDTIE DVLEPYLIQQ GFLQRTPRGR VATLLTYRHF GLSAPD
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag gagatatacc atgggccacc accaccacca ccatagcggc gaagtaaaac cggaagtgaa gccggagacc cacatcaacc tgaaggttag cgacggtagc agcgaaatct tcttcaagat taagaagacc accccgctgc gtcgcctgat ggaagcattc gctaaacgcc agggcaagga aatggattcc ctgcgctttc tgtacgacgg tatccgtatt caggcagacc agactccgga agatctggac atggaagata acgacattat cgaagcacac cgtgaacaaa tcggtggaat gattgagact gacaagctgg ctgccgaacg tattatcgcc gccactcctg ctagcagcca tgaggaagca ttcgaacgtg ctctgcgtcc gcgtcagctt gacgaatacg taggccaaga gaaggtacgc gaccagctgg aaatcttcat tgaagctgcc aaacgtcgtt ccgaggccct tgaccacgtt ctgctgttcg gccctcctgg cctcggcaag accaccctgg cacatatcat cgcacgcgag atgggcgtga atctccgcca gacttccggc cctgtccttg aacgcgctgg cgatctcgct gcactgctga ccaacctcga ggctaacgac gtactgttca ttgacgagat ccaccgcctg tctcctgtcg tggaagagat cctttacccg gccctggagg actaccagat cgatatcatg attggtgaag gtcctgctgc tcgctccgtc aagctggatc tgcagccgtt taccctggtg ggcgctacca cccgtgcagg tatgctgacc aacccgctgc gtgaccgttt cggcatcgtt gctcgtctgg aattctacga cgctgaacaa ctgagccgta tcgttcgccg ttctgccgcc ctgctgaacg ctcagatcga tccggctggc gccctcgaaa tcgctaagcg tagccgcggt actcctcgta tcgctaaccg cctgctgcgt cgtgtgcgtg attacgcaga agttaaggct gacggtaaca tcactgctgc cgtagctgac gcagctctgg ctatgctgga tgttgaccct gtaggcttcg atctgatgga tcgtaagctc ctggaagcta tcctgcataa gttcgacggc ggtccagttg gtgtagataa cctggcagct gccattggcg aagaacgtga caccatcgaa gacgtactgg agccttacct gatccagcaa ggtttcctcc agcgtactcc tcgtggtcgt gttgccaccc tgctgaccta ccgccatttc ggtctgtccg caccagatta aaagcttgcg gccgcactcg agcaccacca ccaccaccac tgagatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggat
Details for BupsA.00013.a.D13.GE00001
HARVESTED ON: data unavailable
SEQUENCED ON: 7/30/2009
EXPECTED MW: 0kDa
OBSERVED MW: data unavailable
ANTIBIOTIC MARKER: kanamycin
COUNT OF EXPRESSION COLONIES: data unavailable
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 12
PERCENT COVERAGE: 81
Validated AA Sequence
MAVGQQGGNT TTRSTLEETL LDQVRLQYVF DGVTFFANGS CQVIYTNWTA VELMQDSFQE LTIHQIEAYR VNIQHSQSCV SYGSSDVTVS LNFCVITHTT QQAVSDTRST AATLSDFEGA SRIDLSVQQG GRTANDTAQL FSVVEFQTSN DAETVTQRVG QHTCTGGSAH QGKRLQIQLD GASSRTFTNH DIDLVVLQGR VKDLFHDRRQ AVDLVNEQYV VSLEVGQQCS EIASAFKDRA GSLAXIHAHL AXDDMCXXVL PXPGGXNSXT XXRASERXLX ASXXIXSWXR XFXXXTXXXX XXXXXXXXNX XXXXXGXXXX XXXXXXXXXX XXXXXTXXXX XXXXXXXXXX
Validated NT Sequence
tncnggcttt gttagcagcc ggatctcagt ggtggtggtg gtgctcgagt gcggccgcaa gcttttagca ctcagcgtca ggagtatccc acaggttgcg taccgggtta gcagcatctg gtgcggacag accgaaatgg cggtaggtca gcagggtggc aacacgacca cgaggagtac gctggaggaa accttgctgg atcaggtaag gctccagtac gtcttcgatg gtgtcacgtt cttcgccaat ggcagctgcc aggttatcta caccaactgg accgccgtcg aacttatgca ggatagcttc caggagctta cgatccatca gatcgaagcc tacagggtca acatccagca tagccagagc tgcgtcagct acggcagcag tgatgttacc gtcagcctta acttctgcgt aatcacgcac acgacgcagc aggcggttag cgatacgagg agtaccgcgg ctacgcttag cgatttcgag ggcgccagcc ggatcgatct gagcgttcag cagggcggca gaacggcgaa cgatacggct cagttgttca gcgtcgtaga attccagacg agcaacgatg ccgaaacggt cacgcagcgg gttggtcagc atacctgcac gggtggtagc gcccaccagg gtaaacggct gcagatccag cttgacggag cgagcagcag gaccttcacc aatcatgata tcgatctggt agtcctccag ggccgggtaa aggatctctt ccacgacagg agacaggcgg tggatctcgt caatgaacag tacgtcgtta gcctcgaggt tggtcagcag tgcagcgaga tcgccagcgc gttcaaggac agggccggaa gtctggcgga nattcacgcc catctcgcgt gngatgatat gtgccnggng gtcttgccga ngccaggagg gncnaacagc anaacgtngn cnagggcctc ngaacgacnt ttggnagctt cnntgaanat ttncagctgg ncgcgtannt tctnnnngnn tacgtantng ncnngctgnn gcnnnnnnnn nncacntncg aatgnnncnn ngatnnnncn nggnnnnnnn nntcnnnnnn ntnnnnnnnn natnnnnnnn cnnnnnnncn ngnnncnnnn nacnnnaann nnnnnnnnnn tcnnntnnnn cnngnnnnnn nnnnnggg
Expected Protein Sequence
MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGAFER ALRPRQLDEY VGQEKVRDQL EIFIEAAKRR SEALDHVLLF GPPGLGKTTL AHIIAREMGV NLRQTSGPVL ERAGDLAALL TNLEANDVLF IDEIHRLSPV VEEILYPALE DYQIDIMIGE GPAARSVKLD LQPFTLVGAT TRAGMLTNPL RDRFGIVARL EFYDAEQLSR IVRRSAALLN AQIDPAGALE IAKRSRGTPR IANRLLRRVR DYAEVKADGN ITAAVADAAL AMLDVDPVGF DLMDRKLLEA ILHKFDGGPV GVDNLAAAIG EERDTIEDVL EPYLIQQGFL QRTPRGRVAT LLTYRHFGLS APDAANPVRN LWDTPDAEC
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag gagatatacc atgggccacc accaccacca ccatagcggc gaagtaaaac cggaagtgaa gccggagacc cacatcaacc tgaaggttag cgacggtagc agcgaaatct tcttcaagat taagaagacc accccgctgc gtcgcctgat ggaagcattc gctaaacgcc agggcaagga aatggattcc ctgcgctttc tgtacgacgg tatccgtatt caggcagacc agactccgga agatctggac atggaagata acgacattat cgaagcacac cgtgaacaaa tcggtggagc attcgaacgt gctctgcgtc cgcgtcagct tgacgaatac gtaggccaag agaaggtacg cgaccagctg gaaatcttca ttgaagctgc caaacgtcgt tccgaggccc ttgaccacgt tctgctgttc ggccctcctg gcctcggcaa gaccaccctg gcacatatca tcgcacgcga gatgggcgtg aatctccgcc agacttccgg ccctgtcctt gaacgcgctg gcgatctcgc tgcactgctg accaacctcg aggctaacga cgtactgttc attgacgaga tccaccgcct gtctcctgtc gtggaagaga tcctttaccc ggccctggag gactaccaga tcgatatcat gattggtgaa ggtcctgctg ctcgctccgt caagctggat ctgcagccgt ttaccctggt gggcgctacc acccgtgcag gtatgctgac caacccgctg cgtgaccgtt tcggcatcgt tgctcgtctg gaattctacg acgctgaaca actgagccgt atcgttcgcc gttctgccgc cctgctgaac gctcagatcg atccggctgg cgccctcgaa atcgctaagc gtagccgcgg tactcctcgt atcgctaacc gcctgctgcg tcgtgtgcgt gattacgcag aagttaaggc tgacggtaac atcactgctg ccgtagctga cgcagctctg gctatgctgg atgttgaccc tgtaggcttc gatctgatgg atcgtaagct cctggaagct atcctgcata agttcgacgg cggtccagtt ggtgtagata acctggcagc tgccattggc gaagaacgtg acaccatcga agacgtactg gagccttacc tgatccagca aggtttcctc cagcgtactc ctcgtggtcg tgttgccacc ctgctgacct accgccattt cggtctgtcc gcaccagatg ctgctaaccc ggtacgcaac ctgtgggata ctcctgacgc tgagtgctaa aagcttgcgg ccgcactcga gcaccaccac caccaccact gagatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggat
Details for BupsA.00013.a.D11.PD00050
PURIFICATION DATe: 5/6/2009
CONCENTRATION: 25mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20mM Tris-HCl, pH 8.0, 200mM NaCl, 1% glycerol, 1mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: 100µl
PERCENT IDENTITY: 14
PERCENT COVERAGE: 75
Protocol Notes
notes unavailable
Validated AA Sequence
MAVGQQGGNT TTRSTLEETL LDQVRLQYVF DGVTFFANGS CQVIYTNWTA VELMQDSFQE LTIHQIEAYR VNIQHSQSCV SYGSSDVTVS LNFCVITHTT QQAVSDTRST AATLSDFEGA SRIDLSVQQG GRTANDTAQL FSVVEFQTSN DAETVTQRVG QHTCTGGSAH QGKRLQIQLD GASSRTFTNH DIDLVVLQGR VKDLFHDRRQ AVDLVNEQYV VSLEVGQQCS EIASAFKDRA GSLAXXHAXL ACDDMCXGGL AEARRAXQXN VXXGLGTTFG SFXXIXSWXX TXXXXTXSSS XXXXXXXXXX XXWXXXXXXX YXXXXXXXXX XXXXXXXXX
Validated NT Sequence
ttcnggcttt gttagcagcc ggntctcngt ggtggtggtg gtggtgctcg agtgcggccg caagctttta gcactcagcg tcaggagtat cccacaggtt gcgtaccggg ttagcagcat ctggtgcgga cagaccgaaa tggcggtagg tcagcagggt ggcaacacga ccacgaggag tacgctggag gaaaccttgc tggatcaggt aaggctccag tacgtcttcg atggtgtcac gttcttcgcc aatggcagct gccaggttat ctacaccaac tggaccgccg tcgaacttat gcaggatagc ttccaggagc ttacgatcca tcagatcgaa gcctacaggg tcaacatcca gcatagccag agctgcgtca gctacggcag cagtgatgtt accgtcagcc ttaacttctg cgtaatcacg cacacgacgc agcaggcggt tagcgatacg aggagtaccg cggctacgct tagcgatttc gagggcgcca gccggatcga tctgagcgtt cagcagggcg gcagaacggc gaacgatacg gctcagttgt tcagcgtcgt agaattccag acgagcaacg atgccgaaac ggtcacgcag cgggttggtc agcatacctg cacgggtggt agcgcccacc agggtaaacg gctgcagatc cagcttgacg gagcgagcag caggaccttc accaatcatg atatcgatct ggtagtcctc cagggccggg taaaggatct cttccacgac aggagacagg cggtggatct cgtcaatgaa cagtacgtcg ttagcctcga ggttggtcag cagtgcagcg agatcgccag cgcgttcaag gacagggccg gaagtctggc gganantcac gccnatctcg cgtgcgatga tatgtgccnn ggnggtcttg ccgaggccag gagggccnaa cagcanaacg tggncanggg cctcggaacg acgtttggna gcttcntgna natttnnagc tggncnngta cnnncnnntn gnnnacgtan tcgtcaagct nanncnnnnn nnnnncncnt nnnaanncnt nnnnatggnn nntannnnnn nnnnnnnnnt acntnnngna nnnnnnnnnn nannctnann nnnnnnnnnn nnnnnnnnnn nnnnnntaat nnnnnnnnnn nnnnnggnnn nnnncngnnn nnnnng
Expressed Protein Sequence
MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGMIET DKLAAERIIA ATPASSHEEA FERALRPRQL DEYVGQEKVR DQLEIFIEAA KRRSEALDHV LLFGPPGLGK TTLAHIIARE MGVNLRQTSG PVLERAGDLA ALLTNLEAND VLFIDEIHRL SPVVEEILYP ALEDYQIDIM IGEGPAARSV KLDLQPFTLV GATTRAGMLT NPLRDRFGIV ARLEFYDAEQ LSRIVRRSAA LLNAQIDPAG ALEIAKRSRG TPRIANRLLR RVRDYAEVKA DGNITAAVAD AALAMLDVDP VGFDLMDRKL LEAILHKFDG GPVGVDNLAA AIGEERDTIE DVLEPYLIQQ GFLQRTPRGR VATLLTYRHF GLSAPDAANP VRNLWDTPDA EC
Full NT Sequence (Expression Vector + Insert)
TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AATTAATTCT TAGAAAAACT CATCGAGCAT CAAATGAAAC TGCAATTTAT TCATATCAGG ATTATCAATA CCATATTTTT GAAAAAGCCG TTTCTGTAAT GAAGGAGAAA ACTCACCGAG GCAGTTCCAT AGGATGGCAA GATCCTGGTA TCGGTCTGCG ATTCCGACTC GTCCAACATC AATACAACCT ATTAATTTCC CCTCGTCAAA AATAAGGTTA TCAAGTGAGA AATCACCATG AGTGACGACT GAATCCGGTG AGAATGGCAA AAGTTTATGC ATTTCTTTCC AGACTTGTTC AACAGGCCAG CCATTACGCT CGTCATCAAA ATCACTCGCA TCAACCAAAC CGTTATTCAT TCGTGATTGC GCCTGAGCGA GACGAAATAC GCGATCGCTG TTAAAAGGAC AATTACAAAC AGGAATCGAA TGCAACCGGC GCAGGAACAC TGCCAGCGCA TCAACAATAT TTTCACCTGA ATCAGGATAT TCTTCTAATA CCTGGAATGC TGTTTTCCCG GGGATCGCAG TGGTGAGTAA CCATGCATCA TCAGGAGTAC GGATAAAATG CTTGATGGTC GGAAGAGGCA TAAATTCCGT CAGCCAGTTT AGTCTGACCA TCTCATCTGT AACATCATTG GCAACGCTAC CTTTGCCATG TTTCAGAAAC AACTCTGGCG CATCGGGCTT CCCATACAAT CGATAGATTG TCGCACCTGA TTGCCCGACA TTATCGCGAG CCCATTTATA CCCATATAAA TCAGCATCCA TGTTGGAATT TAATCGCGGC CTAGAGCAAG ACGTTTCCCG TTGAATATGG CTCATAACAC CCCTTGTATT ACTGTTTATG TAAGCAGACA GTTTTATTGT TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGAGATC CCGGTGCCTA ATGAGTGAGC TAACTTACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCCAG GGTGGTTTTT CTTTTCACCA GTGAGACGGG CAACAGCTGA TTGCCCTTCA CCGCCTGGCC CTGAGAGAGT TGCAGCAAGC GGTCCACGCT GGTTTGCCCC AGCAGGCGAA AATCCTGTTT GATGGTGGTT AACGGCGGGA TATAACATGA GCTGTCTTCG GTATCGTCGT ATCCCACTAC CGAGATATCC GCACCAACGC GCAGCCCGGA CTCGGTAATG GCGCGCATTG CGCCCAGCGC CATCTGATCG TTGGCAACCA GCATCGCAGT GGGAACGATG CCCTCATTCA GCATTTGCAT GGTTTGTTGA AAACCGGACA TGGCACTCCA GTCGCCTTCC CGTTCCGCTA TCGGCTGAAT TTGATTGCGA GTGAGATATT TATGCCAGCC AGCCAGACGC AGACGCGCCG AGACAGAACT TAATGGGCCC GCTAACAGCG CGATTTGCTG GTGACCCAAT GCGACCAGAT GCTCCACGCC CAGTCGCGTA CCGTCTTCAT GGGAGAAAAT AATACTGTTG ATGGGTGTCT GGTCAGAGAC ATCAAGAAAT AACGCCGGAA CATTAGTGCA GGCAGCTTCC ACAGCAATGG CATCCTGGTC ATCCAGCGGA TAGTTAATGA TCAGCCCACT GACGCGTTGC GCGAGAAGAT TGTGCACCGC CGCTTTACAG GCTTCGACGC CGCTTCGTTC TACCATCGAC ACCACCACGC TGGCACCCAG TTGATCGGCG CGAGATTTAA TCGCCGCGAC AATTTGCGAC GGCGCGTGCA GGGCCAGACT GGAGGTGGCA ACGCCAATCA GCAACGACTG TTTGCCCGCC AGTTGTTGTG CCACGCGGTT GGGAATGTAA TTCAGCTCCG CCATCGCCGC TTCCACTTTT TCCCGCGTTT TCGCAGAAAC GTGGCTGGCC TGGTTCACCA CGCGGGAAAC GGTCTGATAA GAGACACCGG CATACTCTGC GACATCGTAT AACGTTACTG GTTTCACATT CACCACCCTG AATTGACTCT CTTCCGGGCG CTATCATGCC ATACCGCGAA AGGTTTTGCG CCATTCGATG GTGTCCGGGA TCTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AATTAATACG ACTCACTATA GGGGAATTGT GAGCGGATAA CAATTCCCCT CTAGAAATAA TTTTGTTTAA CTTTAAGAAG GAGATATACC ATGGGCCACC ACCACCACCA CCATAGCGGC GAAGTAAAAC CGGAAGTGAA GCCGGAGACC CACATCAACC TGAAGGTTAG CGACGGTAGC AGCGAAATCT TCTTCAAGAT TAAGAAGACC ACCCCGCTGC GTCGCCTGAT GGAAGCATTC GCTAAACGCC AGGGCAAGGA AATGGATTCC CTGCGCTTTC TGTACGACGG TATCCGTATT CAGGCAGACC AGACTCCGGA AGATCTGGAC ATGGAAGATA ACGACATTAT CGAAGCACAC CGTGAACAAA TCGGTGGAAT GATTGAGACT GACAAGCTGG CTGCCGAACG TATTATCGCC GCCACTCCTG CTAGCAGCCA TGAGGAAGCA TTCGAACGTG CTCTGCGTCC GCGTCAGCTT GACGAATACG TAGGCCAAGA GAAGGTACGC GACCAGCTGG AAATCTTCAT TGAAGCTGCC AAACGTCGTT CCGAGGCCCT TGACCACGTT CTGCTGTTCG GCCCTCCTGG CCTCGGCAAG ACCACCCTGG CACATATCAT CGCACGCGAG ATGGGCGTGA ATCTCCGCCA GACTTCCGGC CCTGTCCTTG AACGCGCTGG CGATCTCGCT GCACTGCTGA CCAACCTCGA GGCTAACGAC GTACTGTTCA TTGACGAGAT CCACCGCCTG TCTCCTGTCG TGGAAGAGAT CCTTTACCCG GCCCTGGAGG ACTACCAGAT CGATATCATG ATTGGTGAAG GTCCTGCTGC TCGCTCCGTC AAGCTGGATC TGCAGCCGTT TACCCTGGTG GGCGCTACCA CCCGTGCAGG TATGCTGACC AACCCGCTGC GTGACCGTTT CGGCATCGTT GCTCGTCTGG AATTCTACGA CGCTGAACAA CTGAGCCGTA TCGTTCGCCG TTCTGCCGCC CTGCTGAACG CTCAGATCGA TCCGGCTGGC GCCCTCGAAA TCGCTAAGCG TAGCCGCGGT ACTCCTCGTA TCGCTAACCG CCTGCTGCGT CGTGTGCGTG ATTACGCAGA AGTTAAGGCT GACGGTAACA TCACTGCTGC CGTAGCTGAC GCAGCTCTGG CTATGCTGGA TGTTGACCCT GTAGGCTTCG ATCTGATGGA TCGTAAGCTC CTGGAAGCTA TCCTGCATAA GTTCGACGGC GGTCCAGTTG GTGTAGATAA CCTGGCAGCT GCCATTGGCG AAGAACGTGA CACCATCGAA GACGTACTGG AGCCTTACCT GATCCAGCAA GGTTTCCTCC AGCGTACTCC TCGTGGTCGT GTTGCCACCC TGCTGACCTA CCGCCATTTC GGTCTGTCCG CACCAGATGC TGCTAACCCG GTACGCAACC TGTGGGATAC TCCTGACGCT GAGTGCTAAA AGCTTGCGGC CGCACTCGAG CACCACCACC ACCACCACTG AGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGAT
Details for BupsA.00013.a.D11.PD00051
PURIFICATION DATe: 5/6/2009
CONCENTRATION: 15mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20mM Tris-HCl, pH 8.0, 200mM NaCl, 1% glycerol, 1mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 2
VIAL VOLUME: 100µl
PERCENT IDENTITY: 14
PERCENT COVERAGE: 75
Protocol Notes
notes unavailable
Validated AA Sequence
MAVGQQGGNT TTRSTLEETL LDQVRLQYVF DGVTFFANGS CQVIYTNWTA VELMQDSFQE LTIHQIEAYR VNIQHSQSCV SYGSSDVTVS LNFCVITHTT QQAVSDTRST AATLSDFEGA SRIDLSVQQG GRTANDTAQL FSVVEFQTSN DAETVTQRVG QHTCTGGSAH QGKRLQIQLD GASSRTFTNH DIDLVVLQGR VKDLFHDRRQ AVDLVNEQYV VSLEVGQQCS EIASAFKDRA GSLAXXHAXL ACDDMCXGGL AEARRAXQXN VXXGLGTTFG SFXXIXSWXX TXXXXTXSSS XXXXXXXXXX XXWXXXXXXX YXXXXXXXXX XXXXXXXXX
Validated NT Sequence
ttcnggcttt gttagcagcc ggntctcngt ggtggtggtg gtggtgctcg agtgcggccg caagctttta gcactcagcg tcaggagtat cccacaggtt gcgtaccggg ttagcagcat ctggtgcgga cagaccgaaa tggcggtagg tcagcagggt ggcaacacga ccacgaggag tacgctggag gaaaccttgc tggatcaggt aaggctccag tacgtcttcg atggtgtcac gttcttcgcc aatggcagct gccaggttat ctacaccaac tggaccgccg tcgaacttat gcaggatagc ttccaggagc ttacgatcca tcagatcgaa gcctacaggg tcaacatcca gcatagccag agctgcgtca gctacggcag cagtgatgtt accgtcagcc ttaacttctg cgtaatcacg cacacgacgc agcaggcggt tagcgatacg aggagtaccg cggctacgct tagcgatttc gagggcgcca gccggatcga tctgagcgtt cagcagggcg gcagaacggc gaacgatacg gctcagttgt tcagcgtcgt agaattccag acgagcaacg atgccgaaac ggtcacgcag cgggttggtc agcatacctg cacgggtggt agcgcccacc agggtaaacg gctgcagatc cagcttgacg gagcgagcag caggaccttc accaatcatg atatcgatct ggtagtcctc cagggccggg taaaggatct cttccacgac aggagacagg cggtggatct cgtcaatgaa cagtacgtcg ttagcctcga ggttggtcag cagtgcagcg agatcgccag cgcgttcaag gacagggccg gaagtctggc gganantcac gccnatctcg cgtgcgatga tatgtgccnn ggnggtcttg ccgaggccag gagggccnaa cagcanaacg tggncanggg cctcggaacg acgtttggna gcttcntgna natttnnagc tggncnngta cnnncnnntn gnnnacgtan tcgtcaagct nanncnnnnn nnnnncncnt nnnaanncnt nnnnatggnn nntannnnnn nnnnnnnnnt acntnnngna nnnnnnnnnn nannctnann nnnnnnnnnn nnnnnnnnnn nnnnnntaat nnnnnnnnnn nnnnnggnnn nnnncngnnn nnnnng
Expressed Protein Sequence
MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGMIET DKLAAERIIA ATPASSHEEA FERALRPRQL DEYVGQEKVR DQLEIFIEAA KRRSEALDHV LLFGPPGLGK TTLAHIIARE MGVNLRQTSG PVLERAGDLA ALLTNLEAND VLFIDEIHRL SPVVEEILYP ALEDYQIDIM IGEGPAARSV KLDLQPFTLV GATTRAGMLT NPLRDRFGIV ARLEFYDAEQ LSRIVRRSAA LLNAQIDPAG ALEIAKRSRG TPRIANRLLR RVRDYAEVKA DGNITAAVAD AALAMLDVDP VGFDLMDRKL LEAILHKFDG GPVGVDNLAA AIGEERDTIE DVLEPYLIQQ GFLQRTPRGR VATLLTYRHF GLSAPDAANP VRNLWDTPDA EC
Full NT Sequence (Expression Vector + Insert)
TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AATTAATTCT TAGAAAAACT CATCGAGCAT CAAATGAAAC TGCAATTTAT TCATATCAGG ATTATCAATA CCATATTTTT GAAAAAGCCG TTTCTGTAAT GAAGGAGAAA ACTCACCGAG GCAGTTCCAT AGGATGGCAA GATCCTGGTA TCGGTCTGCG ATTCCGACTC GTCCAACATC AATACAACCT ATTAATTTCC CCTCGTCAAA AATAAGGTTA TCAAGTGAGA AATCACCATG AGTGACGACT GAATCCGGTG AGAATGGCAA AAGTTTATGC ATTTCTTTCC AGACTTGTTC AACAGGCCAG CCATTACGCT CGTCATCAAA ATCACTCGCA TCAACCAAAC CGTTATTCAT TCGTGATTGC GCCTGAGCGA GACGAAATAC GCGATCGCTG TTAAAAGGAC AATTACAAAC AGGAATCGAA TGCAACCGGC GCAGGAACAC TGCCAGCGCA TCAACAATAT TTTCACCTGA ATCAGGATAT TCTTCTAATA CCTGGAATGC TGTTTTCCCG GGGATCGCAG TGGTGAGTAA CCATGCATCA TCAGGAGTAC GGATAAAATG CTTGATGGTC GGAAGAGGCA TAAATTCCGT CAGCCAGTTT AGTCTGACCA TCTCATCTGT AACATCATTG GCAACGCTAC CTTTGCCATG TTTCAGAAAC AACTCTGGCG CATCGGGCTT CCCATACAAT CGATAGATTG TCGCACCTGA TTGCCCGACA TTATCGCGAG CCCATTTATA CCCATATAAA TCAGCATCCA TGTTGGAATT TAATCGCGGC CTAGAGCAAG ACGTTTCCCG TTGAATATGG CTCATAACAC CCCTTGTATT ACTGTTTATG TAAGCAGACA GTTTTATTGT TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGAGATC CCGGTGCCTA ATGAGTGAGC TAACTTACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCCAG GGTGGTTTTT CTTTTCACCA GTGAGACGGG CAACAGCTGA TTGCCCTTCA CCGCCTGGCC CTGAGAGAGT TGCAGCAAGC GGTCCACGCT GGTTTGCCCC AGCAGGCGAA AATCCTGTTT GATGGTGGTT AACGGCGGGA TATAACATGA GCTGTCTTCG GTATCGTCGT ATCCCACTAC CGAGATATCC GCACCAACGC GCAGCCCGGA CTCGGTAATG GCGCGCATTG CGCCCAGCGC CATCTGATCG TTGGCAACCA GCATCGCAGT GGGAACGATG CCCTCATTCA GCATTTGCAT GGTTTGTTGA AAACCGGACA TGGCACTCCA GTCGCCTTCC CGTTCCGCTA TCGGCTGAAT TTGATTGCGA GTGAGATATT TATGCCAGCC AGCCAGACGC AGACGCGCCG AGACAGAACT TAATGGGCCC GCTAACAGCG CGATTTGCTG GTGACCCAAT GCGACCAGAT GCTCCACGCC CAGTCGCGTA CCGTCTTCAT GGGAGAAAAT AATACTGTTG ATGGGTGTCT GGTCAGAGAC ATCAAGAAAT AACGCCGGAA CATTAGTGCA GGCAGCTTCC ACAGCAATGG CATCCTGGTC ATCCAGCGGA TAGTTAATGA TCAGCCCACT GACGCGTTGC GCGAGAAGAT TGTGCACCGC CGCTTTACAG GCTTCGACGC CGCTTCGTTC TACCATCGAC ACCACCACGC TGGCACCCAG TTGATCGGCG CGAGATTTAA TCGCCGCGAC AATTTGCGAC GGCGCGTGCA GGGCCAGACT GGAGGTGGCA ACGCCAATCA GCAACGACTG TTTGCCCGCC AGTTGTTGTG CCACGCGGTT GGGAATGTAA TTCAGCTCCG CCATCGCCGC TTCCACTTTT TCCCGCGTTT TCGCAGAAAC GTGGCTGGCC TGGTTCACCA CGCGGGAAAC GGTCTGATAA GAGACACCGG CATACTCTGC GACATCGTAT AACGTTACTG GTTTCACATT CACCACCCTG AATTGACTCT CTTCCGGGCG CTATCATGCC ATACCGCGAA AGGTTTTGCG CCATTCGATG GTGTCCGGGA TCTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AATTAATACG ACTCACTATA GGGGAATTGT GAGCGGATAA CAATTCCCCT CTAGAAATAA TTTTGTTTAA CTTTAAGAAG GAGATATACC ATGGGCCACC ACCACCACCA CCATAGCGGC GAAGTAAAAC CGGAAGTGAA GCCGGAGACC CACATCAACC TGAAGGTTAG CGACGGTAGC AGCGAAATCT TCTTCAAGAT TAAGAAGACC ACCCCGCTGC GTCGCCTGAT GGAAGCATTC GCTAAACGCC AGGGCAAGGA AATGGATTCC CTGCGCTTTC TGTACGACGG TATCCGTATT CAGGCAGACC AGACTCCGGA AGATCTGGAC ATGGAAGATA ACGACATTAT CGAAGCACAC CGTGAACAAA TCGGTGGAAT GATTGAGACT GACAAGCTGG CTGCCGAACG TATTATCGCC GCCACTCCTG CTAGCAGCCA TGAGGAAGCA TTCGAACGTG CTCTGCGTCC GCGTCAGCTT GACGAATACG TAGGCCAAGA GAAGGTACGC GACCAGCTGG AAATCTTCAT TGAAGCTGCC AAACGTCGTT CCGAGGCCCT TGACCACGTT CTGCTGTTCG GCCCTCCTGG CCTCGGCAAG ACCACCCTGG CACATATCAT CGCACGCGAG ATGGGCGTGA ATCTCCGCCA GACTTCCGGC CCTGTCCTTG AACGCGCTGG CGATCTCGCT GCACTGCTGA CCAACCTCGA GGCTAACGAC GTACTGTTCA TTGACGAGAT CCACCGCCTG TCTCCTGTCG TGGAAGAGAT CCTTTACCCG GCCCTGGAGG ACTACCAGAT CGATATCATG ATTGGTGAAG GTCCTGCTGC TCGCTCCGTC AAGCTGGATC TGCAGCCGTT TACCCTGGTG GGCGCTACCA CCCGTGCAGG TATGCTGACC AACCCGCTGC GTGACCGTTT CGGCATCGTT GCTCGTCTGG AATTCTACGA CGCTGAACAA CTGAGCCGTA TCGTTCGCCG TTCTGCCGCC CTGCTGAACG CTCAGATCGA TCCGGCTGGC GCCCTCGAAA TCGCTAAGCG TAGCCGCGGT ACTCCTCGTA TCGCTAACCG CCTGCTGCGT CGTGTGCGTG ATTACGCAGA AGTTAAGGCT GACGGTAACA TCACTGCTGC CGTAGCTGAC GCAGCTCTGG CTATGCTGGA TGTTGACCCT GTAGGCTTCG ATCTGATGGA TCGTAAGCTC CTGGAAGCTA TCCTGCATAA GTTCGACGGC GGTCCAGTTG GTGTAGATAA CCTGGCAGCT GCCATTGGCG AAGAACGTGA CACCATCGAA GACGTACTGG AGCCTTACCT GATCCAGCAA GGTTTCCTCC AGCGTACTCC TCGTGGTCGT GTTGCCACCC TGCTGACCTA CCGCCATTTC GGTCTGTCCG CACCAGATGC TGCTAACCCG GTACGCAACC TGTGGGATAC TCCTGACGCT GAGTGCTAAA AGCTTGCGGC CGCACTCGAG CACCACCACC ACCACCACTG AGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGAT