BupsA.00010.g

acetoacetyl-CoA reductase

CENTER ID: BupsA.00010.g
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: in PDB
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.00010.g.A1.GE25426 Full length( BupsA.00010.g ) 1 248
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Structures

3GK3
DEPOSITED: 3/9/2009
DETERMINATION: XRay
CLONE: BupsA.00010.g.A1.GE25426
PROTEIN: BupsA.00010.g.A1.PS00076
5VT6
DEPOSITED: 5/15/2017
DETERMINATION: XRay
CLONE: BupsA.00010.g.A1.GE25426
PROTEIN: BupsA.00010.g.A1.PS00076

Publications by SSGCID

Combining functional and structural genomics to sample the essential Burkholderia structome.
Abendroth J, Armour B, Barrett L, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Fox D, Gallagher LA, Gardberg AS, Gillespie A, Manoil C, Myler PJ, Nakazawa-Hewitt S, Napuli A, Nguyen MT, Patrapuvich R, Phan I, Stacy R, Staker BL, Stewart LJ, Van Voorhis WC
PLoS ONE - 2012
volume 8, issue 1, pages e53851
PMID: 23382856; PMCID: PMC3561365
PDB: 3D63, 3DAH, 3EIZ, 3EJ2, 3EK2, 3EZO, 3F0F, 3FTP, 3GK0, 3GK3, 3GVF, 3GWA, 3GWE, 3IML, 3SZ8, 3T4C, 3TML, 3TMQ, 3TXY, 3U7J, 3UE9, 3UK1, 3UK2, 3UND, 3UPT, 3URR, 3UW1, 3UW2, 3UW3, 3V2I, 3V7N, 3V8H, 3V9O, 3V9P, 3VAV, 4DDO, 4DFE, 4DHE, 4DHK, 4DUT, 4DZ4, 4E4T, 4E51, 4EFI, 4EG0, 4EGJ, 4EK2, 4EQY, 4EWG, 4EX5, 4EXQ, 4F2G, 4F32, 4F3N, 4F3Y, 4F4H, 4F7D, 4FK8, 4FRY, 4G1K, 4G67, 4G6Z, 4GHK, 4H3Y, 4H3Z, 4H4G

External Resources

RESOURCE REFERENCE ID
OrthoMCL: OG5_129142
PATRIC ID: fig|320372.6.peg.5074
RefSeq: YP_336172.1
UniProt: Q3JJT1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQAKRVAFVT GGMGGLGAAI SRRLHDAGMA VAVSHSERND HVSTWLMHER DAGRDFKAYA VDVADFESCE RCAEKVLADF GKVDVLINNA GITRDATFMK MTKGDWDAVM RTDLDAMFNV TKQFIAGMVE RRFGRIVNIG SVNGSRGAFG QANYASAKAG IHGFTKTLAL ETAKRGITVN TVSPGYLATA MVEAVPQDVL EAKILPQIPV GRLGRPDEVA ALIAFLCSDD AGFVTGADLA INGGMHMS
NT Sequence
ATGCAAGCAA AACGCGTGGC ATTCGTGACG GGCGGTATGG GCGGACTGGG TGCGGCGATC AGCCGGCGGC TGCACGACGC CGGCATGGCT GTGGCGGTGT CGCATTCGGA GCGCAACGAT CATGTGTCGA CGTGGCTGAT GCACGAGCGC GACGCCGGAC GCGATTTCAA GGCGTATGCG GTCGACGTCG CGGATTTCGA ATCGTGCGAG CGGTGCGCGG AGAAGGTGCT CGCCGATTTC GGCAAGGTCG ACGTGCTGAT CAACAACGCG GGGATCACGC GCGATGCGAC GTTCATGAAG ATGACGAAGG GCGACTGGGA CGCGGTGATG CGCACCGATC TCGATGCGAT GTTCAACGTG ACGAAACAGT TCATCGCGGG CATGGTCGAG CGGCGCTTCG GGCGCATCGT CAACATCGGT TCGGTGAACG GCTCGCGCGG CGCGTTCGGT CAGGCGAATT ATGCGTCGGC GAAGGCGGGC ATCCACGGTT TCACGAAGAC GCTCGCGCTC GAGACGGCGA AGCGCGGGAT CACGGTCAAC ACGGTGTCGC CCGGCTATCT CGCGACCGCG ATGGTCGAAG CGGTGCCGCA GGACGTGCTC GAGGCGAAGA TCCTGCCGCA GATTCCGGTT GGCCGGCTCG GCCGGCCCGA CGAAGTGGCC GCGCTCATCG CGTTTCTGTG CTCGGACGAC GCGGGGTTCG TCACCGGCGC CGATCTCGCG ATCAACGGCG GGATGCACAT GTCC
Details for BupsA.00010.g.A1.GE25426
HARVESTED ON: 6/30/2008
SEQUENCED ON: 3/16/2012
EXPECTED MW: 28kDa
OBSERVED MW: 29kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMQAKRVAFV TGGMGGLGAA ISRRLHDAGM AVAVSHSERN DHVSTWLMHE RDAGRDFKAY AVDVADFESC ERCAEKVLAD FGKVDVLINN AGITRDATFM KMTKGDWDAV MRTDLDAMFN VTKQFIAGMV ERRFGRIVNI GSVNGSRGAF GQANYASAKA GIHGFTKTLA LETAKRGITV NTVSPGYLAT AMVEAVPQDV LEAKILPQIP VGRLGRPDEV AALIAFLCSD DAGFVTGADL AINGGMHMS
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgcaag caaaacgcgt ggcattcgtg acgggcggta tgggcggact gggtgcggcg atcagccggc ggctgcacga cgccggcatg gctgtggcgg tgtcgcattc ggagcgcaac gatcatgtgt cgacgtggct gatgcacgag cgcgacgccg gacgcgattt caaggcgtat gcggtcgacg tcgcggattt cgaatcgtgc gagcggtgcg cggagaaggt gctcgccgat ttcggcaagg tcgacgtgct gatcaacaac gcggggatca cgcgcgatgc gacgttcatg aagatgacga agggcgactg ggacgcggtg atgcgcaccg atctcgatgc gatgttcaac gtgacgaaac agttcatcgc gggcatggtc gagcggcgct tcgggcgcat cgtcaacatc ggttcggtga acggctcgcg cggcgcgttc ggtcaggcga attatgcgtc ggcgaaggcg ggcatccacg gtttcacgaa gacgctcgcg ctcgagacgg cgaagcgcgg gatcacggtc aacacggtgt cgcccggcta tctcgcgacc gcgatggtcg aagcggtgcc gcaggacgtg ctcgaggcga agatcctgcc gcagattccg gttggccggc tcggccggcc cgacgaagtg gccgcgctca tcgcgtttct gtgctcggac gacgcggggt tcgtcaccgg cgccgatctc gcgatcaacg gcgggatgca catgtcctaa taa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMQAKRVAFV TGGMGGLGAA ISRRLHDAGM AVAVSHSERN DHVSTWLMHE RDAGRDFKAY AVDVADFESC ERCAEKVLAD FGKVDVLINN AGITRDATFM KMTKGDWDAV MRTDLDAMFN VTKQFIAGMV ERRFGRIVNI GSVNGSRGAF GQANYASAKA GIHGFTKTLA LETAKRGITV NTVSPGYLAT AMVEAVPQDV LEAKILPQIP VGRLGRPDEV AALIAFLCSD DAGFVTGADL AINGGMHMS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcaagcaaaa cgcgtggcat tcgtgacggg cggtatgggc ggactgggtg cggcgatcag ccggcggctg cacgacgccg gcatggctgt ggcggtgtcg cattcggagc gcaacgatca tgtgtcgacg tggctgatgc acgagcgcga cgccggacgc gatttcaagg cgtatgcggt cgacgtcgcg gatttcgaat cgtgcgagcg gtgcgcggag aaggtgctcg ccgatttcgg caaggtcgac gtgctgatca acaacgcggg gatcacgcgc gatgcgacgt tcatgaagat gacgaagggc gactgggacg cggtgatgcg caccgatctc gatgcgatgt tcaacgtgac gaaacagttc atcgcgggca tggtcgagcg gcgcttcggg cgcatcgtca acatcggttc ggtgaacggc tcgcgcggcg cgttcggtca ggcgaattat gcgtcggcga aggcgggcat ccacggtttc acgaagacgc tcgcgctcga gacggcgaag cgcgggatca cggtcaacac ggtgtcgccc ggctatctcg cgaccgcgat ggtcgaagcg gtgccgcagg acgtgctcga ggcgaagatc ctgccgcaga ttccggttgg ccggctcggc cggcccgacg aagtggccgc gctcatcgcg tttctgtgct cggacgacgc ggggttcgtc accggcgccg atctcgcgat caacggcggg atgcacatgt ccaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa