BupsA.00010.e
acetyacetyl-CoA reductase
| CENTER ID: | BupsA.00010.e |
| ORGANISM: | Burkholderia pseudomallei 1710b |
| ASSOCIATED DISEASE: | Melioidosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BupsA.00010.e.A1.GE25424 | Full length( BupsA.00010.e ) | 1 | 248 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
3EZL
DEPOSITED: 10/23/2008
DETERMINATION: XRay
CLONE: BupsA.00010.e.A1.GE25424
PROTEIN: BupsA.00010.e.A1.PS00061
5VML
DEPOSITED: 4/27/2017
DETERMINATION: XRay
CLONE: BupsA.00010.e.A1.GE25424
PROTEIN: BupsA.00010.e.A1.PS00061
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|320372.6.peg.2436 |
| RefSeq: | YP_333724.1 |
| UniProt: | Q3JRS9 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVMSQRIAYV TGGMGGIGTS ICQRLHKDGF RVVAGCGPNS PRRVKWLEDQ KALGFDFYAS EGNVGDWDST KQAFDKVKAE VGEIDVLVNN AGITRDVVFR KMTREDWQAV IDTNLTSLFN VTKQVIDGMV ERGWGRIINI SSVNGQKGQF GQTNYSTAKA GIHGFTMSLA QEVATKGVTV NTVSPGYIGT DMVKAIRPDV LEKIVATIPV RRLGSPDEIG SIVAWLASEE SGFSTGADFS LNGGLHMG
NT Sequence
ATGGTTATGT CTCAGCGAAT TGCTTACGTA ACGGGCGGCA TGGGCGGCAT CGGCACGAGC ATCTGCCAGC GCCTGCACAA GGACGGCTTC AGGGTGGTCG CGGGCTGCGG CCCGAATTCG CCGCGCCGCG TGAAATGGCT CGAGGATCAG AAGGCGCTCG GCTTCGATTT CTACGCGTCC GAAGGCAACG TCGGCGACTG GGATTCGACG AAGCAGGCGT TCGACAAGGT GAAGGCCGAA GTGGGCGAGA TCGACGTGCT CGTCAATAAC GCGGGCATCA CGCGCGACGT CGTGTTCCGC AAGATGACCC GCGAGGATTG GCAGGCGGTG ATCGACACGA ACCTGACGAG CCTCTTCAAC GTCACGAAGC AGGTGATCGA CGGGATGGTC GAGCGCGGCT GGGGGCGCAT CATCAACATC TCGTCGGTGA ACGGCCAGAA GGGGCAGTTC GGCCAGACCA ACTATTCGAC CGCGAAGGCG GGCATTCACG GCTTCACGAT GTCGCTCGCG CAGGAAGTCG CGACGAAGGG GGTGACGGTC AACACGGTGT CGCCGGGCTA CATCGGCACG GACATGGTGA AGGCGATTCG GCCGGACGTG CTCGAGAAGA TCGTCGCGAC GATTCCGGTG CGCCGTCTCG GCTCGCCCGA CGAGATCGGC TCGATCGTCG CGTGGCTCGC GTCGGAAGAG TCCGGGTTCT CGACGGGCGC CGATTTTTCG CTGAACGGCG GCTTGCATAT GGGC
Details for BupsA.00010.e.A1.GE25424
| HARVESTED ON: | 6/30/2008 |
| SEQUENCED ON: | 3/16/2012 |
| EXPECTED MW: | 29kDa |
| OBSERVED MW: | 30kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVMSQRIAY VTGGMGGIGT SICQRLHKDG FRVVAGCGPN SPRRVKWLED QKALGFDFYA SEGNVGDWDS TKQAFDKVKA EVGEIDVLVN NAGITRDVVF RKMTREDWQA VIDTNLTSLF NVTKQVIDGM VERGWGRIIN ISSVNGQKGQ FGQTNYSTAK AGIHGFTMSL AQEVATKGVT VNTVSPGYIG TDMVKAIRPD VLEKIVATIP VRRLGSPDEI GSIVAWLASE ESGFSTGADF SLNGGLHMG
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtta tgtctcagcg aattgcttac gtaacgggcg gcatgggcgg catcggcacg agcatctgcc agcgcctgca caaggacggc ttcagggtgg tcgcgggctg cggcccgaat tcgccgcgcc gcgtgaaatg gctcgaggat cagaaggcgc tcggcttcga tttctacgcg tccgaaggca acgtcggcga ctgggattcg acgaagcagg cgttcgacaa ggtgaaggcc gaagtgggcg agatcgacgt gctcgtcaat aacgcgggca tcacgcgcga cgtcgtgttc cgcaagatga cccgcgagga ttggcaggcg gtgatcgaca cgaacctgac gagcctcttc aacgtcacga agcaggtgat cgacgggatg gtcgagcgcg gctgggggcg catcatcaac atctcgtcgg tgaacggcca gaaggggcag ttcggccaga ccaactattc gaccgcgaag gcgggcattc acggcttcac gatgtcgctc gcgcaggaag tcgcgacgaa gggggtgacg gtcaacacgg tgtcgccggg ctacatcggc acggacatgg tgaaggcgat tcggccggac gtgctcgaga agatcgtcgc gacgattccg gtgcgccgtc tcggctcgcc cgacgagatc ggctcgatcg tcgcgtggct cgcgtcggaa gagtccgggt tctcgacggg cgccgatttt tcgctgaacg gcggcttgca tatgggctaa taa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVMSQRIAY VTGGMGGIGT SICQRLHKDG FRVVAGCGPN SPRRVKWLED QKALGFDFYA SEGNVGDWDS TKQAFDKVKA EVGEIDVLVN NAGITRDVVF RKMTREDWQA VIDTNLTSLF NVTKQVIDGM VERGWGRIIN ISSVNGQKGQ FGQTNYSTAK AGIHGFTMSL AQEVATKGVT VNTVSPGYIG TDMVKAIRPD VLEKIVATIP VRRLGSPDEI GSIVAWLASE ESGFSTGADF SLNGGLHMG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggttatgtct cagcgaattg cttacgtaac gggcggcatg ggcggcatcg gcacgagcat ctgccagcgc ctgcacaagg acggcttcag ggtggtcgcg ggctgcggcc cgaattcgcc gcgccgcgtg aaatggctcg aggatcagaa ggcgctcggc ttcgatttct acgcgtccga aggcaacgtc ggcgactggg attcgacgaa gcaggcgttc gacaaggtga aggccgaagt gggcgagatc gacgtgctcg tcaataacgc gggcatcacg cgcgacgtcg tgttccgcaa gatgacccgc gaggattggc aggcggtgat cgacacgaac ctgacgagcc tcttcaacgt cacgaagcag gtgatcgacg ggatggtcga gcgcggctgg gggcgcatca tcaacatctc gtcggtgaac ggccagaagg ggcagttcgg ccagaccaac tattcgaccg cgaaggcggg cattcacggc ttcacgatgt cgctcgcgca ggaagtcgcg acgaaggggg tgacggtcaa cacggtgtcg ccgggctaca tcggcacgga catggtgaag gcgattcggc cggacgtgct cgagaagatc gtcgcgacga ttccggtgcg ccgtctcggc tcgcccgacg agatcggctc gatcgtcgcg tggctcgcgt cggaagagtc cgggttctcg acgggcgccg atttttcgct gaacggcggc ttgcatatgg gcaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa