SSGCID: Target Page

BupsA.00010.c

enoyl-(acyl carrier protein) reductase

CENTER ID:	BupsA.00010.c
ORGANISM:	Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE:	Melioidosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.00010.c.A1.GE25422	Full length( BupsA.00010.c )		1	253

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|320372.6.peg.6484
RefSeq:	YP_337448.1
UniProt:	Q3JG55

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRLQHKRGLI IGIANENSIA FGCARVMREQ GAELALTYLN EKAEPYVRPL AQRLDSRLVV PCDVREPGRL EDVFARIAQE WGQLDFVLHS IAYAPKEDLH RRVTDCSQAG FAMAMDVSCH SFIRVARLAE PLMTNGGCLL TVTFYGAERA VEDYNLMGPV KAALEGSVRY LAAELGPRRI RVHALSPGPL KTRAASGIDR FDALLERVRE RTPGHRLVDI DDVGHVAAFL ASDDAAALTG NVEYIDGGYH VVG

NT Sequence

ATGCGACTTC AGCACAAGCG TGGCCTCATC ATCGGCATTG CAAACGAGAA CAGCATCGCG TTCGGCTGCG CGCGGGTCAT GCGCGAGCAG GGCGCCGAGC TCGCGCTCAC TTACTTGAAC GAAAAGGCCG AGCCCTATGT CCGTCCGCTC GCGCAGCGTC TGGACAGCCG GCTCGTCGTG CCGTGCGACG TGCGCGAGCC CGGCCGGCTC GAGGACGTGT TCGCGCGCAT CGCGCAGGAA TGGGGGCAGC TCGACTTCGT GCTGCACTCG ATCGCATACG CGCCGAAGGA GGACCTGCAT CGCCGCGTGA CCGACTGCTC GCAGGCGGGC TTCGCGATGG CGATGGACGT GTCGTGCCAT TCGTTCATTC GCGTCGCGCG CCTGGCCGAG CCGCTGATGA CGAACGGCGG CTGCCTGCTG ACCGTCACGT TCTACGGTGC CGAGCGGGCT GTCGAGGACT ACAACCTGAT GGGCCCGGTG AAGGCGGCGC TCGAGGGCTC GGTGCGCTAT CTCGCCGCCG AGCTCGGGCC GCGGCGCATC CGCGTGCACG CGCTGTCGCC CGGGCCGCTG AAGACGCGCG CCGCGTCCGG CATCGACCGC TTCGACGCGC TGCTCGAACG CGTGCGCGAG CGCACGCCCG GGCATCGCCT CGTCGACATC GACGACGTCG GCCATGTCGC CGCGTTCCTC GCGAGCGACG ATGCGGCCGC GCTGACGGGC AACGTCGAGT ACATCGACGG CGGCTATCAC GTCGTCGGC

Details for BupsA.00010.c.A1.GE25422

HARVESTED ON:	6/30/2008
SEQUENCED ON:	3/16/2012
EXPECTED MW:	30kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMRLQHKRGL IIGIANENSI AFGCARVMRE QGAELALTYL NEKAEPYVRP LAQRLDSRLV VPCDVREPGR LEDVFARIAQ EWGQLDFVLH SIAYAPKEDL HRRVTDCSQA GFAMAMDVSC HSFIRVARLA EPLMTNGGCL LTVTFYGAER AVEDYNLMGP VKAALEGSVR YLAAELGPRR IRVHALSPGP LKTRAASGID RFDALLERVR ERTPGHRLVD IDDVGHVAAF LASDDAAALT GNVEYIDGGY HVVG

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgcgac ttcagcacaa gcgtggcctc atcatcggca ttgcaaacga gaacagcatc gcgttcggct gcgcgcgggt catgcgcgag cagggcgccg agctcgcgct cacttacttg aacgaaaagg ccgagcccta tgtccgtccg ctcgcgcagc gtctggacag ccggctcgtc gtgccgtgcg acgtgcgcga gcccggccgg ctcgaggacg tgttcgcgcg catcgcgcag gaatgggggc agctcgactt cgtgctgcac tcgatcgcat acgcgccgaa ggaggacctg catcgccgcg tgaccgactg ctcgcaggcg ggcttcgcga tggcgatgga cgtgtcgtgc cattcgttca ttcgcgtcgc gcgcctggcc gagccgctga tgacgaacgg cggctgcctg ctgaccgtca cgttctacgg tgccgagcgg gctgtcgagg actacaacct gatgggcccg gtgaaggcgg cgctcgaggg ctcggtgcgc tatctcgccg ccgagctcgg gccgcggcgc atccgcgtgc acgcgctgtc gcccgggccg ctgaagacgc gcgccgcgtc cggcatcgac cgcttcgacg cgctgctcga acgcgtgcgc gagcgcacgc ccgggcatcg cctcgtcgac atcgacgacg tcggccatgt cgccgcgttc ctcgcgagcg acgatgcggc cgcgctgacg ggcaacgtcg agtacatcga cggcggctat cacgtcgtcg gctaataa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcgacttcag cacaagcgtg gcctcatcat cggcattgca aacgagaaca gcatcgcgtt cggctgcgcg cgggtcatgc gcgagcaggg cgccgagctc gcgctcactt acttgaacga aaaggccgag ccctatgtcc gtccgctcgc gcagcgtctg gacagccggc tcgtcgtgcc gtgcgacgtg cgcgagcccg gccggctcga ggacgtgttc gcgcgcatcg cgcaggaatg ggggcagctc gacttcgtgc tgcactcgat cgcatacgcg ccgaaggagg acctgcatcg ccgcgtgacc gactgctcgc aggcgggctt cgcgatggcg atggacgtgt cgtgccattc gttcattcgc gtcgcgcgcc tggccgagcc gctgatgacg aacggcggct gcctgctgac cgtcacgttc tacggtgccg agcgggctgt cgaggactac aacctgatgg gcccggtgaa ggcggcgctc gagggctcgg tgcgctatct cgccgccgag ctcgggccgc ggcgcatccg cgtgcacgcg ctgtcgcccg ggccgctgaa gacgcgcgcc gcgtccggca tcgaccgctt cgacgcgctg ctcgaacgcg tgcgcgagcg cacgcccggg catcgcctcg tcgacatcga cgacgtcggc catgtcgccg cgttcctcgc gagcgacgat gcggccgcgc tgacgggcaa cgtcgagtac atcgacggcg gctatcacgt cgtcggcaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa