BupsA.00010.b
enoyl-(acyl carrier protein) reductase
| CENTER ID: | BupsA.00010.b |
| ORGANISM: | Burkholderia pseudomallei 1710b |
| ASSOCIATED DISEASE: | Melioidosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
Clicking the button will add the material to a virtual cart.
You may order multiple materials at a time at no cost to you, as this contract is funded
by NIAID. When you are ready to place your order, click the "Place Order" link which will
appear in the top right corner of the page after you place your first item in your cart.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BupsA.00010.b.B1.PS00034 | Full length( BupsA.00010.b ) | 1 | 263 |
Structures
3EK2
DEPOSITED: 9/18/2008
DETERMINATION: XRay
CLONE: BupsA.00010.b.B1.GE25083
PROTEIN: BupsA.00010.b.B1.PS00034
5TRT
DEPOSITED: 10/27/2016
DETERMINATION: XRay
CLONE: BupsA.00010.b.B1.GE25083
PROTEIN: BupsA.00010.b.B1.PS00034
Publications by SSGCID
Combining functional and structural genomics to sample the essential Burkholderia structome.
Abendroth J, Armour B, Barrett L, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Fox D, Gallagher LA, Gardberg AS, Gillespie A, Manoil C, Myler PJ, Nakazawa-Hewitt S, Napuli A, Nguyen MT, Patrapuvich R, Phan I, Stacy R, Staker BL, Stewart LJ, Van Voorhis WC
PLoS ONE - 2012
volume 8, issue 1, pages e53851
PMID: 23382856; PMCID: PMC3561365
PDB:
3D63,
3DAH,
3EIZ,
3EJ2,
3EK2,
3EZO,
3F0F,
3FTP,
3GK0,
3GK3,
3GVF,
3GWA,
3GWE,
3IML,
3SZ8,
3T4C,
3TML,
3TMQ,
3TXY,
3U7J,
3UE9,
3UK1,
3UK2,
3UND,
3UPT,
3URR,
3UW1,
3UW2,
3UW3,
3V2I,
3V7N,
3V8H,
3V9O,
3V9P,
3VAV,
4DDO,
4DFE,
4DHE,
4DHK,
4DUT,
4DZ4,
4E4T,
4E51,
4EFI,
4EG0,
4EGJ,
4EK2,
4EQY,
4EWG,
4EX5,
4EXQ,
4F2G,
4F32,
4F3N,
4F3Y,
4F4H,
4F7D,
4FK8,
4FRY,
4G1K,
4G67,
4G6Z,
4GHK,
4H3Y,
4H3Z,
4H4G
Citations by Others
| Rational Optimization of Diphenyl Ether Binding Kinetics to the Enoyl-ACP Reductase FabI1 from Burkholderia pseudomallei | |
| C Neckles, S Eltschkner, JE Cummings- Biochemistry, 2017 - ncbi.nlm.nih.gov | |
| cited by: | 1 other |
| PDB: 3EK2 | |
| Crystalline Structure of FABI from Burkholderia Pseudomallei | |
| NR Krishnamurthy - US Patent , 2017 - freepatentsonline.com | |
| PDB: 3EK2 |
| Rationalizing the Binding Kinetics for the Inhibition of the Burkholderia pseudomallei FabI1 Enoyl-ACP Reductase | |
| C Neckles, S Eltschkner, JE Cummings - Biochemistry, 2017 - ACS Publications | |
| PDB: 3EK2 |
| Pyridone FabI Inhibitors and Uses Thereof | |
| P Tonge - US Patent App. 15/130,365, 2016 - Google Patents | |
| PDB: 3EK2 |
| Rare Sidechain Conformations in Proteins and DNA | |
| BJ Hintze - 2015 - search.proquest.com | |
| PDB: 3EK2 |
| AFN‐1252 is a potent inhibitor of Enoyl‐ACP reductase from Burkholderia pseudomallei‐Crystal structure, Mode of action and biological activity | |
| KN Rao, A Lakshminarasimhan, S Joseph… - Protein …, 2015 - Wiley Online Library | |
| PDB: 3EK2 |
| Rational Design of Broad Spectrum Antibacterial Activity Based on a Clinically Relevant Enoyl-Acyl Carrier Protein (ACP) Reductase Inhibitor | |
| J Schiebel, A Chang, S Shah, Y Lu, L Liu, P Pan - Journal of Biological Chemistry, 2014 - ASBMB | |
| cited by: | 13 others |
| PDB: 3EK2 | |
| Paclitaxel Induces Apoptosis in Breast Cancer Cells through Different Calcium-Regulating Mechanisms Depending on External Calcium Conditions | |
| Z Pan, A Avila, L Gollahon - International journal of molecular sciences, 2014 - mdpi.com | |
| PDB: 3EK2 |
| < i> Staphylococcus aureus FabI: Inhibition, Substrate Recognition, and Potential Implications for In Vivo Essentiality | |
| J Schiebel, A Chang, H Lu, MV Baxter, PJ Tonge? - Structure, 2012 - Elsevier | |
| cited by: | 33 others |
| PDB: 3EK2 | |
| High-resolution structures of Thermus thermophilus enoyl-acyl carrier protein reductase in the apo form, in complex with NAD+ and in complex with NAD+ and triclosan | |
| JM Otero, AJ Noel, P Guardado-Calvo? - Acta Crystallographica Section F Structural Biology and Crystallization Communications, 2012 - scripts.iucr.org | |
| cited by: | 2 others |
| PDB: 3EK2 | |
| Structure-based drug design on the enoyl-ACP reductases of Yersinia pestis and Burkholderia pseudomallei | |
| MW Hirschbeck - opus.uni-wuerzburg.de | |
| PDB: 3EK2 |
| Structural insights into Staphylococcus aureus enoyl-ACP reductase (FabI), in complex with NADP and triclosan | |
| A Priyadarshi, EEK Kim? - Proteins: Structure, Function, and Bioinformatics, 2010 - Wiley Online Library | |
| cited by: | 23 others |
| PDB: 3EK2 | |
This list was obtained from Google Scholar searches using the Open Source tool https://github.com/ckreibich/scholar.py and each citation has been manually reviewed.
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|320372.6.peg.2759 |
| RefSeq: | YP_334023 |
| UniProt: | Q3JQY0 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGFLDGKRIL LTGLLSNRSI AYGIAKACKR EGAELAFTYV GDRFKDRITE FAAEFGSELV FPCDVADDAQ IDALFASLKT HWDSLDGLVH SIGFAPREAI AGDFLDGLTR ENFRIAHDIS AYSFPALAKA ALPMLSDDAS LLTLSYLGAE RAIPNYNTMG LAKAALEASV RYLAVSLGAK GVRVNAISAG PIKTLAASGI KSFGKILDFV ESNSPLKRNV TIEQVGNAGA FLLSDLASGV TAEVMHVDSG FNAVVGGMAG LEE
NT Sequence
ATGGGCTTTC TCGACGGTAA ACGTATTCTG CTGACGGGCC TCTTGTCGAA CCGTTCGATC GCTTACGGCA TCGCCAAGGC GTGCAAGCGC GAAGGCGCCG AGCTGGCGTT CACCTACGTC GGCGATCGCT TCAAGGATCG CATCACCGAG TTCGCGGCCG AGTTCGGCAG CGAACTCGTG TTCCCGTGCG ATGTCGCCGA CGATGCGCAG ATCGATGCGC TCTTCGCGTC GCTGAAGACG CACTGGGATT CGCTCGACGG CCTCGTCCAC TCGATCGGCT TCGCGCCGCG CGAGGCGATC GCGGGCGACT TCCTCGACGG CCTCACGCGC GAGAACTTCC GCATCGCGCA CGACATCTCC GCATACAGCT TCCCCGCGCT CGCGAAGGCG GCGCTGCCGA TGCTGTCGGA CGATGCGTCG CTGCTCACGC TGTCGTATCT CGGCGCGGAG CGGGCGATCC CGAACTACAA CACGATGGGC CTCGCGAAGG CGGCGCTCGA GGCGAGCGTG CGCTATCTCG CGGTGTCGCT CGGCGCGAAG GGCGTGCGCG TGAACGCGAT CTCGGCGGGC CCGATCAAGA CGCTCGCGGC AAGCGGCATC AAGAGCTTCG GCAAGATCCT CGATTTCGTC GAGAGCAACT CGCCGCTCAA GCGCAACGTG ACGATCGAGC AGGTCGGCAA CGCGGGCGCG TTCCTGCTGT CGGACCTCGC GAGCGGCGTC ACGGCCGAAG TCATGCACGT CGACTCGGGC TTCAACGCGG TGGTGGGCGG GATGGCCGGC CTCGAGGAA
Details for BupsA.00010.b.B1.PS00034
| PURIFICATION DATe: | 7/1/2008 |
| CONCENTRATION: | 25.05mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 22 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG FLDGKRILLT GLLSNRSIAY GIAKACKREG AELAFTYVGD RFKDRITEFA AEFGSELVFP CDVADDAQID ALFASLKTHW DSLDGLVHSI GFAPREAIAG DFLDGLTREN FRIAHDISAY SFPALAKAAL PMLSDDASLL TLSYLGAERA IPNYNTMGLA KAALEASVRY LAVSLGAKGV RVNAISAGPI KTLAASGIKS FGKILDFVES NSPLKRNVTI EQVGNAGAFL LSDLASGVTA EVMHVDSGFN AVVGGMAGLE E
Validated NT Sequence
tcttactcac ttattcctcg aggccggcca tcccgcccac caccgcgttg aagcccgagt cgacgtgcat gacttcggcc gtgacgccgc tcgcgaggtc cgacagcagg aacgcgcccg cgttgccgac ctgctcgatc gtcacgttgc gcttgagcgg cgagttgctc tcgacgaaat cgaggatctt gccgaagctc ttgatgccgc ttgccgcgag cgtcttgatc gggcccgccg agatcgcgtt cacgcgcacg cccttcgcgc cgagcgacac cgcgagatag cgcacgctcg cctcgagcgc cgccttcgcg aggcccatcg tgttgtagtt cgggatcgcc cgctccgcgc cgagatacga cagcgtgagc agcgacgcat cgtccgacag catcggcagc gccgccttcg cgagcgcggg gaagctgtat gcggagatgt cgtgcgcgat gcggaagttc tcgcgcgtga ggccgtcgag gaagtcgccc gcgatcgcct cgcgcggcgc gaagccgatc gagtggacga ggccgtcgag cgaatcccag tgcgtcttca gcgacgcgaa gagcgcatcg atctgcgcat cgtcggcgac atcgcacggg aacacgagtt cgctgccgaa ctcggccgcg aactcggtga tgcgatcctt gaagcgatcg ccgacgtagg tgaacgccag ctcggcgcct tcgcgcttgc acgccttggc gatgccgtaa gcgatcgaac ggttcgacaa gaggcccgtc agcagaatac gtttaccgtc gagaaagccc atatggtggt ggtggtggtg agccat
Expressed Protein Sequence
MAHHHHHHMG FLDGKRILLT GLLSNRSIAY GIAKACKREG AELAFTYVGD RFKDRITEFA AEFGSELVFP CDVADDAQID ALFASLKTHW DSLDGLVHSI GFAPREAIAG DFLDGLTREN FRIAHDISAY SFPALAKAAL PMLSDDASLL TLSYLGAERA IPNYNTMGLA KAALEASVRY LAVSLGAKGV RVNAISAGPI KTLAASGIKS FGKILDFVES NSPLKRNVTI EQVGNAGAFL LSDLASGVTA EVMHVDSGFN AVVGGMAGLE E
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGGCT TTCTCGACGG TAAACGTATT CTGCTGACGG GCCTCTTGTC GAACCGTTCG ATCGCTTACG GCATCGCCAA GGCGTGCAAG CGCGAAGGCG CCGAGCTGGC GTTCACCTAC GTCGGCGATC GCTTCAAGGA TCGCATCACC GAGTTCGCGG CCGAGTTCGG CAGCGAACTC GTGTTCCCGT GCGATGTCGC CGACGATGCG CAGATCGATG CGCTCTTCGC GTCGCTGAAG ACGCACTGGG ATTCGCTCGA CGGCCTCGTC CACTCGATCG GCTTCGCGCC GCGCGAGGCG ATCGCGGGCG ACTTCCTCGA CGGCCTCACG CGCGAGAACT TCCGCATCGC GCACGACATC TCCGCATACA GCTTCCCCGC GCTCGCGAAG GCGGCGCTGC CGATGCTGTC GGACGATGCG TCGCTGCTCA CGCTGTCGTA TCTCGGCGCG GAGCGGGCGA TCCCGAACTA CAACACGATG GGCCTCGCGA AGGCGGCGCT CGAGGCGAGC GTGCGCTATC TCGCGGTGTC GCTCGGCGCG AAGGGCGTGC GCGTGAACGC GATCTCGGCG GGCCCGATCA AGACGCTCGC GGCAAGCGGC ATCAAGAGCT TCGGCAAGAT CCTCGATTTC GTCGAGAGCA ACTCGCCGCT CAAGCGCAAC GTGACGATCG AGCAGGTCGG CAACGCGGGC GCGTTCCTGC TGTCGGACCT CGCGAGCGGC GTCACGGCCG AAGTCATGCA CGTCGACTCG GGCTTCAACG CGGTGGTGGG CGGGATGGCC GGCCTCGAGG AATAATGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT