BupsA.00010.a
3-ketoacyl-(acyl-carrier-protein) reductase
| CENTER ID: | BupsA.00010.a |
| ORGANISM: | Burkholderia pseudomallei 1710b |
| ASSOCIATED DISEASE: | Melioidosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BupsA.00010.a.A1.GE24986 | Full length( BupsA.00010.a ) | 1 | 249 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BupsA.00010.a.B1.PS00013 | Full length( BupsA.00010.a ) | 1 | 249 |
Structures
3FTP
DEPOSITED: 1/13/2009
DETERMINATION: XRay
CLONE:
PROTEIN: BupsA.00010.a.A1.PS0011
Publications by SSGCID
Combining functional and structural genomics to sample the essential Burkholderia structome.
Abendroth J, Armour B, Barrett L, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Fox D, Gallagher LA, Gardberg AS, Gillespie A, Manoil C, Myler PJ, Nakazawa-Hewitt S, Napuli A, Nguyen MT, Patrapuvich R, Phan I, Stacy R, Staker BL, Stewart LJ, Van Voorhis WC
PLoS ONE - 2012
volume 8, issue 1, pages e53851
PMID: 23382856; PMCID: PMC3561365
PDB:
3D63,
3DAH,
3EIZ,
3EJ2,
3EK2,
3EZO,
3F0F,
3FTP,
3GK0,
3GK3,
3GVF,
3GWA,
3GWE,
3IML,
3SZ8,
3T4C,
3TML,
3TMQ,
3TXY,
3U7J,
3UE9,
3UK1,
3UK2,
3UND,
3UPT,
3URR,
3UW1,
3UW2,
3UW3,
3V2I,
3V7N,
3V8H,
3V9O,
3V9P,
3VAV,
4DDO,
4DFE,
4DHE,
4DHK,
4DUT,
4DZ4,
4E4T,
4E51,
4EFI,
4EG0,
4EGJ,
4EK2,
4EQY,
4EWG,
4EX5,
4EXQ,
4F2G,
4F32,
4F3N,
4F3Y,
4F4H,
4F7D,
4FK8,
4FRY,
4G1K,
4G67,
4G6Z,
4GHK,
4H3Y,
4H3Z,
4H4G
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_126618 |
| PATRIC ID: | fig|320372.6.peg.3036 |
| RefSeq: | YP_334286 |
| UniProt: | Q3JQ67 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDKTLDKQVA IVTGASRGIG RAIALELARR GAMVIGTATT EAGAEGIGAA FKQAGLEGRG AVLNVNDATA VDALVESTLK EFGALNVLVN NAGITQDQLA MRMKDDEWDA VIDTNLKAVF RLSRAVLRPM MKARGGRIVN ITSVVGSAGN PGQVNYAAAK AGVAGMTRAL AREIGSRGIT VNCVAPGFID TDMTKGLPQE QQTALKTQIP LGRLGSPEDI AHAVAFLASP QAGYITGTTL HVNGGMFMS
NT Sequence
ATGGACAAGA CTCTCGACAA ACAAGTCGCG ATCGTCACCG GCGCGTCGCG CGGGATCGGC CGCGCGATCG CGCTCGAACT CGCGCGCCGC GGCGCAATGG TGATCGGCAC CGCGACGACC GAAGCGGGCG CCGAGGGCAT CGGCGCCGCG TTCAAGCAAG CCGGGCTCGA AGGGCGCGGC GCCGTGCTGA ACGTCAATGA CGCAACGGCG GTCGATGCGC TCGTCGAATC GACGCTCAAG GAGTTCGGCG CGCTGAACGT GCTCGTCAAC AATGCCGGCA TCACGCAGGA TCAGCTCGCG ATGCGGATGA AGGACGACGA GTGGGACGCC GTGATCGACA CGAATCTGAA GGCGGTGTTC CGTCTGTCGC GCGCGGTGCT GCGCCCGATG ATGAAGGCGC GCGGCGGACG CATCGTCAAT ATCACGTCGG TCGTCGGCTC CGCGGGCAAT CCGGGTCAGG TCAACTACGC GGCGGCGAAG GCCGGCGTCG CGGGAATGAC GCGCGCGCTC GCGCGCGAAA TCGGCAGCCG CGGCATCACC GTGAACTGCG TCGCGCCGGG CTTCATCGAT ACCGACATGA CGAAGGGCCT GCCGCAAGAG CAGCAGACGG CGCTGAAGAC CCAGATTCCG CTCGGCCGCC TGGGCAGCCC GGAGGACATC GCGCACGCGG TGGCGTTCCT CGCGTCGCCG CAGGCGGGCT ACATCACGGG CACGACGCTG CACGTGAACG GCGGCATGTT CATGTCG
Details for BupsA.00010.a.A1.GE24986
| HARVESTED ON: | 3/31/2008 |
| SEQUENCED ON: | 6/16/2009 |
| EXPECTED MW: | 28kDa |
| OBSERVED MW: | 28kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 98 |
| PERCENT COVERAGE: | 99 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMDKTLDKQV AIVTGASRGI GRAIALELAR RGAMVIGTAT TEAGAEGIGA AFKQAGLEGR GAVLNVNDAT AVDALVESTL KEFGALNVLV NNAGITQDQL AMRMKDDEWD AVIDTNLKAV FRLSRAVLRP MMKARGGRIV NITSVVGSAG NPGQVNYAAA KAGVAGMTRA LAREIGSRGI TVNCVAPGFI DTDMTKGLPQ EQQTALKTXI PLGRLGSPXD IAHAVAFLAS PXAGYXTGTT LHVNGGMFM
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggaca agactctcga caaacaagtc gcgatcgtca ccggcgcgtc gcgcgggatc ggccgcgcga tcgcgctcga actcgcgcgc cgcggcgcaa tggtgatcgg caccgcgacg accgaagcgg gcgccgaggg catcggcgcc gcgttcaagc aagccgggct cgaagggcgc ggcgccgtgc tgaacgtcaa tgacgcaacg gcggtcgatg cgctcgtcga atcgacgctc aaggagttcg gcgcgctgaa cgtgctcgtc aacaatgccg gcatcacgca ggatcagctc gcgatgcgga tgaaggacga cgagtgggac gccgtgatcg acacgaatct gaaggcggtg ttccgtctgt cgcgcgcggt gctgcgcccg atgatgaagg cgcgcggcgg acgcatcgtc aatatcacgt cggtcgtcgg ctccgcgggc aatccgggtc aggtcaacta cgcggcggcg aaggccggcg tcgcgggaat gacgcgcgcg ctcgcgcgcg aaatcggcag ccgcggcatc accgtgaact gcgtcgcgcc gggcttcatc gataccgaca tgacgaaggg nctgccgcaa gagcagcaga cggcgctgaa gacccanatt ccgctcggcc gcctgggcag cccggangac atcgcgcacg cggtggcgtt cctcgcgtcg ccgcangcgg gctacntcac gggcacgacg ctgcacgtga acggcggcat gttcatgtc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDKTLDKQV AIVTGASRGI GRAIALELAR RGAMVIGTAT TEAGAEGIGA AFKQAGLEGR GAVLNVNDAT AVDALVESTL KEFGALNVLV NNAGITQDQL AMRMKDDEWD AVIDTNLKAV FRLSRAVLRP MMKARGGRIV NITSVVGSAG NPGQVNYAAA KAGVAGMTRA LAREIGSRGI TVNCVAPGFI DTDMTKGLPQ EQQTALKTQI PLGRLGSPED IAHAVAFLAS PQAGYITGTT LHVNGGMFMS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggacaagact ctcgacaaac aagtcgcgat cgtcaccggc gcgtcgcgcg ggatcggccg cgcgatcgcg ctcgaactcg cgcgccgcgg cgcaatggtg atcggcaccg cgacgaccga agcgggcgcc gagggcatcg gcgccgcgtt caagcaagcc gggctcgaag ggcgcggcgc cgtgctgaac gtcaatgacg caacggcggt cgatgcgctc gtcgaatcga cgctcaagga gttcggcgcg ctgaacgtgc tcgtcaacaa tgccggcatc acgcaggatc agctcgcgat gcggatgaag gacgacgagt gggacgccgt gatcgacacg aatctgaagg cggtgttccg tctgtcgcgc gcggtgctgc gcccgatgat gaaggcgcgc ggcggacgca tcgtcaatat cacgtcggtc gtcggctccg cgggcaatcc gggtcaggtc aactacgcgg cggcgaaggc cggcgtcgcg ggaatgacgc gcgcgctcgc gcgcgaaatc ggcagccgcg gcatcaccgt gaactgcgtc gcgccgggct tcatcgatac cgacatgacg aagggcctgc cgcaagagca gcagacggcg ctgaagaccc agattccgct cggccgcctg ggcagcccgg aggacatcgc gcacgcggtg gcgttcctcg cgtcgccgca ggcgggctac atcacgggca cgacgctgca cgtgaacggc ggcatgttca tgtcgtaaaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for BupsA.00010.a.B1.PS00013
| PURIFICATION DATe: | 5/15/2008 |
| CONCENTRATION: | 7.97mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 2 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | 92 |
| PERCENT COVERAGE: | 74 |
Protocol Notes
notes unavailable
Validated AA Sequence
MDKTLDKQVA IVTGASRGXX RAIALELARR GAMVIGTATT EAGAEGIGAA FKQAGLEGRG AVLNVNDATA VDALVESTLK EFGALNVLVN NAGXXQDQLA MRMKDDEWDA VIDTNLKAVF RLSRAVLRPM MKARGGRIVN ITSVVGSAGN PGQXXXXAAK AGVAGMTRAL AREIGSRGXT VXCVXXXSIP T
Validated NT Sequence
tnnnntnnct ttnngnagga ganataccnt ggctcaccac caccaccacc atatggacaa gactctcgac aaacaagtcg cgatcgtcac cggcgcgtcg cgcgggnnnn nccgcgcgat cgcgctcgaa ctcgcgcgcc gcggcgcaat ggtgatcggc accgcgacga ccgaagcggg cgccgagggc atcggcgccg cgttcaagca agccgggctc gaagggcgcg gcgccgtgct gaacgtcaat gacgcaacgg cggtcgatgc gctcgtcgaa tcgacgctca aggagttcgg cgcgctgaac gtgctcgtca acaatgccgg cntcncncag gatcagctcg cgatgcggat gaaggacgac gagtgggacg ccgtgatcga cacgaatctg aaggcggtgt tccgtctgtc ncgcgcggtg ctgcgcccga tgatgaaggc gcgcggcgga cgcatcgtca atatcacgtc ggtcgtcggc tccgcgggca atccgggtca ggncaantan nnggcggcga aggccggcgt cgcgggaatg acgcgcgcgc tcgcgcgcga aatcggcagc cgcggnntca ccgtnnnntg cgtcnngncn gnntcgatac cgacntgacn aanggcctgn nnnaagagca nnnnacngtn aagacccnna ttccgctcgg ncgccnngnn cagcccggan gacatncacg cngtgggcgt ttcctcgnnn nnncgcangn gggctncatc ncnggnncna cnntnnntnt tgancngngn cnngtttnnn gtcntaagtg antannnnga tcccngcngc nnancnnnnc cnanannaan ctgantgnnn gnngcccnnn ctganaanan tnananaacc ccnntggngc nnnnaaacgg nnntganggn tttttnnnnn aangnngnan tnnnnncngn nanntcnnnn ggacgnnnnn nnnnnnnnnn natnccnnnn nnnnnannng gnnncnannn cnnannnnnn nnnannnnnn nnnnnncnan nnnnnnnnnn nnnngntncc annannnnng ncncnnnnaa annnnatcnn cncnntnnnn nnntannnac ccncnnnnnn gnactgggna
Expressed Protein Sequence
MAHHHHHHMD KTLDKQVAIV TGASRGIGRA IALELARRGA MVIGTATTEA GAEGIGAAFK QAGLEGRGAV LNVNDATAVD ALVESTLKEF GALNVLVNNA GITQDQLAMR MKDDEWDAVI DTNLKAVFRL SRAVLRPMMK ARGGRIVNIT SVVGSAGNPG QVNYAAAKAG VAGMTRALAR EIGSRGITVN CVAPGFIDTD MTKGLPQEQQ TALKTQIPLG RLGSPEDIAH AVAFLASPQA GYITGTTLHV NGGMFMS
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGACA AGACTCTCGA CAAACAAGTC GCGATCGTCA CCGGCGCGTC GCGCGGGATC GGCCGCGCGA TCGCGCTCGA ACTCGCGCGC CGCGGCGCAA TGGTGATCGG CACCGCGACG ACCGAAGCGG GCGCCGAGGG CATCGGCGCC GCGTTCAAGC AAGCCGGGCT CGAAGGGCGC GGCGCCGTGC TGAACGTCAA TGACGCAACG GCGGTCGATG CGCTCGTCGA ATCGACGCTC AAGGAGTTCG GCGCGCTGAA CGTGCTCGTC AACAATGCCG GCATCACGCA GGATCAGCTC GCGATGCGGA TGAAGGACGA CGAGTGGGAC GCCGTGATCG ACACGAATCT GAAGGCGGTG TTCCGTCTGT CGCGCGCGGT GCTGCGCCCG ATGATGAAGG CGCGCGGCGG ACGCATCGTC AATATCACGT CGGTCGTCGG CTCCGCGGGC AATCCGGGTC AGGTCAACTA CGCGGCGGCG AAGGCCGGCG TCGCGGGAAT GACGCGCGCG CTCGCGCGCG AAATCGGCAG CCGCGGCATC ACCGTGAACT GCGTCGCGCC GGGCTTCATC GATACCGACA TGACGAAGGG CCTGCCGCAA GAGCAGCAGA CGGCGCTGAA GACCCAGATT CCGCTCGGCC GCCTGGGCAG CCCGGAGGAC ATCGCGCACG CGGTGGCGTT CCTCGCGTCG CCGCAGGCGG GCTACATCAC GGGCACGACG CTGCACGTGA ACGGCGGCAT GTTCATGTCG TAATGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT