BupsA.00008.a
serine hydroxymethyltransferase
| CENTER ID: | BupsA.00008.a |
| ORGANISM: | Burkholderia pseudomallei 1710b |
| ASSOCIATED DISEASE: | Melioidosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIB |
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Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BupsA.00008.a.D19.PD00019 | Full length( BupsA.00008.a ) | 1 | 424 |
Structures
3ECD
DEPOSITED: 8/29/2008
DETERMINATION: XRay
CLONE: BupsA.00008.a.D19.GE00001
PROTEIN: BupsA.00008.a.D19.PD00019
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_126679 |
| PATRIC ID: | fig|320372.6.peg.6270 |
| RefSeq: | YP_337258 |
| UniProt: | Q3JGP5 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
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AA Sequence
MSNANPFFSQ SLAERDASVR GAILKELERQ QSQVELIASE NIVSRAVLDA QGSVLTNKYA EGYPGKRYYG GCEFADEVEA LAIERVKRLF NAGHANVQPH SGAQANGAVM LALAKPGDTV LGMSLDAGGH LTHGAKPALS GKWFNALQYG VSRDTMLIDY DQVEALAQQH KPSLIIAGFS AYPRKLDFAR FRAIADSVGA KLMVDMAHIA GVIAAGRHAN PVEHAHVVTS TTHKTLRGPR GGFVLTNDEE IAKKINSAVF PGLQGGPLMH VIAGKAVAFG EALTDDFKTY IDRVLANAQA LGDVLKAGGV DLVTGGTDNH LLLVDLRPKG LKGAQVEQAL ERAGITCNKN GIPFDPEKPT ITSGIRLGTP AGTTRGFGAA EFREVGRLIL EVFEALRTNP EGDHATEQRV RREIFALCER FPIY
NT Sequence
ATGTCGAACG CCAATCCCTT CTTCTCGCAA TCGCTCGCCG AGCGCGACGC GTCCGTGCGC GGCGCGATCC TCAAGGAACT CGAACGCCAG CAGTCGCAAG TCGAGCTGAT CGCGTCGGAG AACATCGTGT CGCGCGCGGT GCTCGATGCG CAGGGCTCGG TGCTGACCAA CAAGTATGCG GAAGGCTATC CGGGCAAGCG CTATTACGGC GGTTGCGAAT TCGCCGACGA AGTCGAGGCG CTCGCGATCG AGCGCGTGAA GCGGCTCTTC AACGCCGGCC ATGCGAACGT GCAGCCGCAC TCGGGCGCCC AGGCGAACGG CGCGGTGATG CTCGCGCTCG CGAAGCCGGG CGACACGGTG CTCGGAATGT CGCTCGACGC GGGCGGACAC CTCACGCACG GCGCGAAGCC GGCGCTGTCC GGCAAGTGGT TCAACGCGCT CCAGTACGGC GTGAGCCGCG ACACGATGCT GATCGATTAC GACCAGGTCG AGGCGCTCGC GCAGCAGCAC AAGCCGAGCC TCATCATCGC CGGCTTCTCC GCCTATCCGC GCAAGCTCGA CTTCGCGCGG TTTCGCGCGA TCGCCGATTC GGTCGGCGCG AAGCTGATGG TCGACATGGC GCATATCGCC GGCGTGATCG CCGCGGGCCG CCATGCGAAC CCGGTCGAAC ACGCGCACGT CGTCACGTCG ACGACGCACA AGACGCTGCG CGGCCCGCGC GGCGGCTTCG TGCTGACCAA CGACGAGGAG ATCGCGAAGA AGATCAACTC GGCGGTGTTC CCCGGCCTGC AGGGCGGCCC GCTGATGCAC GTGATCGCCG GCAAGGCGGT GGCGTTCGGC GAGGCGCTGA CAGACGATTT CAAGACCTAC ATCGATCGCG TGCTCGCGAA CGCGCAGGCG CTCGGCGACG TGCTGAAGGC GGGCGGCGTC GATCTCGTGA CGGGCGGCAC CGACAACCAT CTGCTGCTCG TCGATCTGCG CCCGAAGGGC CTGAAGGGCG CGCAGGTCGA GCAGGCGCTC GAGCGCGCGG GCATCACGTG CAACAAGAAC GGCATTCCGT TCGATCCGGA GAAGCCGACG ATCACGTCGG GCATCCGCCT CGGCACGCCG GCGGGCACGA CGCGCGGCTT CGGCGCAGCG GAGTTCCGCG AGGTCGGCCG GCTGATTCTC GAGGTGTTCG AGGCCCTGCG CACGAACCCG GAAGGCGATC ACGCGACCGA ACAGCGCGTG CGGCGCGAGA TCTTCGCGCT TTGCGAACGC TTCCCGATCT AC
Details for BupsA.00008.a.D19.PD00019
| PURIFICATION DATe: | 6/11/2008 |
| CONCENTRATION: | 24.1mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20mM Tris-HCl, pH 8.0, 200mM NaCl, 1% glycerol, 1mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 50 |
| VIAL VOLUME: | data unavailable |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGSMSN ANPFFSQSLA ERDASVRGAI LKELERQQSQ VELIASENIV SRAVLDAQGS VLTNKYAEGY PGKRYYGGCE FADEVEALAI ERVKRLFNAG HANVQPHSGA QANGAVMLAL AKPGDTVLGM SLDAGGHLTH GAKPALSGKW FNALQYGVSR DTMLIDYDQV EALAQQHKPS LIIAGFSAYP RKLDFARFRA IADSVGAKLM VDMAHIAGVI AAGRHANPVE HAHVVTSTTH KTLRGPRGGF VLTNDEEIAK KINSAVFPGL QGGPLMHVIA GKAVAFGEAL TDDFKTYIDR VLANAQALGD VLKAGGVDLV TGGTDNHLLL VDLRPKGLKG AQVEQALERA GITCNKNGIP FDPEKPTITS GIRLGTPAGT TRGFGAAEFR EVGRLILEVF EALRTNPEGD HATEQRVRRE IFALCERFPI Y
Full NT Sequence (Expression Vector + Insert)
TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AATTAATTCT TAGAAAAACT CATCGAGCAT CAAATGAAAC TGCAATTTAT TCATATCAGG ATTATCAATA CCATATTTTT GAAAAAGCCG TTTCTGTAAT GAAGGAGAAA ACTCACCGAG GCAGTTCCAT AGGATGGCAA GATCCTGGTA TCGGTCTGCG ATTCCGACTC GTCCAACATC AATACAACCT ATTAATTTCC CCTCGTCAAA AATAAGGTTA TCAAGTGAGA AATCACCATG AGTGACGACT GAATCCGGTG AGAATGGCAA AAGTTTATGC ATTTCTTTCC AGACTTGTTC AACAGGCCAG CCATTACGCT CGTCATCAAA ATCACTCGCA TCAACCAAAC CGTTATTCAT TCGTGATTGC GCCTGAGCGA GACGAAATAC GCGATCGCTG TTAAAAGGAC AATTACAAAC AGGAATCGAA TGCAACCGGC GCAGGAACAC TGCCAGCGCA TCAACAATAT TTTCACCTGA ATCAGGATAT TCTTCTAATA CCTGGAATGC TGTTTTCCCG GGGATCGCAG TGGTGAGTAA CCATGCATCA TCAGGAGTAC GGATAAAATG CTTGATGGTC GGAAGAGGCA TAAATTCCGT CAGCCAGTTT AGTCTGACCA TCTCATCTGT AACATCATTG GCAACGCTAC CTTTGCCATG TTTCAGAAAC AACTCTGGCG CATCGGGCTT CCCATACAAT CGATAGATTG TCGCACCTGA TTGCCCGACA TTATCGCGAG CCCATTTATA CCCATATAAA TCAGCATCCA TGTTGGAATT TAATCGCGGC CTAGAGCAAG ACGTTTCCCG TTGAATATGG CTCATAACAC CCCTTGTATT ACTGTTTATG TAAGCAGACA GTTTTATTGT TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGAGATC CCGGTGCCTA ATGAGTGAGC TAACTTACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCCAG GGTGGTTTTT CTTTTCACCA GTGAGACGGG CAACAGCTGA TTGCCCTTCA CCGCCTGGCC CTGAGAGAGT TGCAGCAAGC GGTCCACGCT GGTTTGCCCC AGCAGGCGAA AATCCTGTTT GATGGTGGTT AACGGCGGGA TATAACATGA GCTGTCTTCG GTATCGTCGT ATCCCACTAC CGAGATATCC GCACCAACGC GCAGCCCGGA CTCGGTAATG GCGCGCATTG CGCCCAGCGC CATCTGATCG TTGGCAACCA GCATCGCAGT GGGAACGATG CCCTCATTCA GCATTTGCAT GGTTTGTTGA AAACCGGACA TGGCACTCCA GTCGCCTTCC CGTTCCGCTA TCGGCTGAAT TTGATTGCGA GTGAGATATT TATGCCAGCC AGCCAGACGC AGACGCGCCG AGACAGAACT TAATGGGCCC GCTAACAGCG CGATTTGCTG GTGACCCAAT GCGACCAGAT GCTCCACGCC CAGTCGCGTA CCGTCTTCAT GGGAGAAAAT AATACTGTTG ATGGGTGTCT GGTCAGAGAC ATCAAGAAAT AACGCCGGAA CATTAGTGCA GGCAGCTTCC ACAGCAATGG CATCCTGGTC ATCCAGCGGA TAGTTAATGA TCAGCCCACT GACGCGTTGC GCGAGAAGAT TGTGCACCGC CGCTTTACAG GCTTCGACGC CGCTTCGTTC TACCATCGAC ACCACCACGC TGGCACCCAG TTGATCGGCG CGAGATTTAA TCGCCGCGAC AATTTGCGAC GGCGCGTGCA GGGCCAGACT GGAGGTGGCA ACGCCAATCA GCAACGACTG TTTGCCCGCC AGTTGTTGTG CCACGCGGTT GGGAATGTAA TTCAGCTCCG CCATCGCCGC TTCCACTTTT TCCCGCGTTT TCGCAGAAAC GTGGCTGGCC TGGTTCACCA CGCGGGAAAC GGTCTGATAA GAGACACCGG CATACTCTGC GACATCGTAT AACGTTACTG GTTTCACATT CACCACCCTG AATTGACTCT CTTCCGGGCG CTATCATGCC ATACCGCGAA AGGTTTTGCG CCATTCGATG GTGTCCGGGA TCTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AATTAATACG ACTCACTATA GGGGAATTGT GAGCGGATAA CAATTCCCCT CTAGAAATAA TTTTGTTTAA CTTTAAGAAG GAGATATACC ATGGGCCACC ACCACCACCA CCATAGCGGC GAAGTAAAAC CGGAAGTGAA GCCGGAGACC CACATCAACC TGAAGGTTAG CGACGGTAGC AGCGAAATCT TCTTCAAGAT TAAGAAGACC ACCCCGCTGC GTCGCCTGAT GGAAGCATTC GCTAAACGCC AGGGCAAGGA AATGGATTCC CTGCGCTTTC TGTACGACGG TATCCGTATT CAGGCAGACC AGACTCCGGA AGATCTGGAC ATGGAAGATA ACGACATTAT CGAAGCACAC CGTGAACAAA TCGGTGGATC CATGTCGAAC GCCAATCCCT TCTTCTCGCA ATCGCTCGCC GAGCGCGACG CGTCCGTGCG CGGCGCGATC CTCAAGGAAC TCGAACGCCA GCAGTCGCAA GTCGAGCTGA TCGCGTCGGA GAACATCGTG TCGCGCGCGG TGCTCGATGC GCAGGGCTCG GTGCTGACCA ACAAGTATGC GGAAGGCTAT CCGGGCAAGC GCTATTACGG CGGTTGCGAA TTCGCCGACG AAGTCGAGGC GCTCGCGATC GAGCGCGTGA AGCGGCTCTT CAACGCCGGC CATGCGAACG TGCAGCCGCA CTCGGGTGCC CAGGCGAACG GCGCGGTGAT GCTCGCGCTC GCGAAGCCGG GCGACACGGT GCTCGGAATG TCGCTCGACG CGGGCGGACA CCTCACCCAT GGCGCGAAGC CGGCGCTGTC CGGCAAGTGG TTCAACGCGC TCCAGTACGG CGTGAGCCGC GACACGATGC TGATCGATTA CGACCAGGTC GAGGCGCTCG CGCAGCAGCA CAAGCCGAGC CTCATCATCG CCGGCTTCTC CGCCTATCCG CGCAAGCTCG ACTTCGCGCG GTTTCGCGCG ATCGCCGATT CGGTCGGCGC GAAGCTGATG GTCGACATGG CGCATATCGC CGGCGTGATC GCCGCGGGCC GCCATGCGAA CCCGGTCGAA CACGCGCACG TCGTCACGTC GACGACGCAC AAGACGCTGC GCGGCCCGCG CGGCGGCTTC GTGCTGACCA ACGACGAGGA GATCGCGAAG AAGATCAACT CGGCGGTGTT CCCCGGCCTG CAGGGCGGCC CGCTGATGCA CGTGATCGCC GGCAAGGCGG TGGCGTTCGG CGAGGCGCTG ACAGACGATT TCAAGACCTA CATCGATCGC GTGCTCGCGA ACGCGCAGGC GCTCGGCGAC GTGCTGAAGG CGGGCGGCGT CGATCTCGTG ACGGGCGGCA CCGACAACCA TCTGCTGCTC GTCGATCTGC GCCCGAAGGG CCTGAAGGGC GCGCAGGTCG AGCAGGCGCT CGAGCGCGCG GGCATCACGT GCAACAAGAA CGGCATTCCG TTCGATCCGG AGAAGCCGAC GATCACGTCG GGCATCCGCC TCGGCACGCC GGCGGGCACG ACGCGCGGCT TCGGCGCAGC GGAGTTCCGC GAGGTCGGCC GGCTGATTCT CGAGGTGTTC GAGGCCCTGC GCACGAACCC GGAAGGCGAT CACGCGACCG AACAGCGCGT GCGGCGCGAG ATCTTCGCGC TTTGCGAACG CTTCCCGATC TACTGATAAA AGCTTGCGGC CGCACTCGAG CACCACCACC ACCACCACTG AGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGAT