BupsA.00005.a
Malate dehydrogenase
| CENTER ID: | BupsA.00005.a |
| ORGANISM: | Burkholderia pseudomallei 1710b |
| ASSOCIATED DISEASE: | Melioidosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BupsA.00005.a.A1.GE24983 | Full length( BupsA.00005.a ) | 1 | 327 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BupsA.00005.a.A1.PW25021 | Full length( BupsA.00005.a ) | 1 | 327 |
Structures
3D5T
DEPOSITED: 5/16/2008
DETERMINATION: XRay
CLONE: BupsA.00005.a.A1.GE24983
PROTEIN: BupsA.00005.a.A1.PW25075
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_127410 |
| PATRIC ID: | fig|320372.6.peg.4835 |
| RefSeq: | YP_335954 |
| UniProt: | Q3JKE9 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAKPAKRVAV TGAAGQIAYS LLFRIANGDL LGKDQPVILQ LLDLPQAQAA VKGVVMELDD CAFPLLAGVV ITDDPKVAFK DADVALLVGA RPRSKGMERK DLLSANAEIF TVQGAALNEV ASRDVKVLVV GNPANTNAYI AMKSAPDLPK KNFTAMLRLD HNRALSQLAA KSGKPVASIE KLAVWGNHSP TMYPDFRFAT AEGESLLKLI NDDVWNRDTF IPTVGKRGAA IIEARGLSSA ASAANAAIDH VRDWVLGTNG KWVTMGIPSD GSYGIPEDII YGVPVICENG EYKRVEGLEI DAFSREKMDG TLAELLEERD GVAHLLK
NT Sequence
ATGGCTAAGC CCGCAAAGCG CGTTGCCGTC ACCGGCGCCG CAGGTCAGAT CGCTTACTCG CTGCTGTTTC GCATCGCGAA CGGCGACCTG CTCGGCAAGG ATCAGCCGGT CATCCTGCAA CTGCTCGACC TCCCGCAAGC CCAGGCCGCC GTCAAAGGCG TCGTGATGGA ACTGGACGAT TGCGCGTTCC CGCTGCTCGC GGGCGTCGTG ATCACCGACG ACCCGAAGGT CGCGTTCAAG GACGCCGACG TCGCGCTGCT GGTGGGCGCG CGTCCGCGCT CGAAGGGCAT GGAGCGCAAG GATCTGCTGT CGGCGAACGC CGAGATCTTC ACGGTCCAGG GCGCGGCGCT GAACGAAGTC GCGAGCCGCG ACGTGAAGGT GCTGGTCGTC GGCAACCCGG CGAACACGAA CGCGTACATC GCGATGAAGT CGGCGCCGGA TCTGCCGAAG AAGAACTTCA CGGCGATGCT GCGCCTCGAC CACAACCGCG CGCTGTCGCA GCTCGCCGCG AAGTCGGGCA AGCCGGTCGC GTCGATCGAG AAGCTCGCCG TGTGGGGCAA CCACTCGCCG ACGATGTACC CCGACTTCCG CTTCGCGACC GCCGAGGGCG AATCGCTGCT GAAGCTGATC AACGACGACG TGTGGAACCG CGACACGTTC ATCCCGACTG TCGGCAAGCG CGGCGCGGCG ATCATCGAAG CGCGCGGCCT GTCGTCGGCG GCGTCGGCGG CCAACGCGGC GATCGACCAC GTGCGCGACT GGGTGCTCGG CACGAACGGC AAGTGGGTCA CGATGGGCAT CCCGTCGGAC GGCTCGTACG GCATCCCCGA GGACATCATC TACGGCGTGC CGGTGATTTG CGAAAACGGC GAGTACAAGC GCGTCGAGGG CCTGGAAATC GACGCGTTCT CGCGCGAGAA GATGGACGGC ACGCTCGCCG AGCTGCTCGA GGAGCGCGAC GGCGTCGCCC ATCTGCTGAA G
Details for BupsA.00005.a.A1.GE24983
| HARVESTED ON: | 2/12/2008 |
| SEQUENCED ON: | 6/16/2009 |
| EXPECTED MW: | 37kDa |
| OBSERVED MW: | 37kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAKPAKRVA VTGAAGQIAY SLLFRIANGD LLGKDQPVIL QLLDLPQAQA AVKGVVMELD DCAFPLLAGV VITDDPKVAF KDADVALLVG ARPRSKGMER KDLLSANAEI FTVQGAALNE VASRDVKVLV VGNPANTNAY IAMKSAPDLP KKNFTAMLRL DHNRALSQLA AKSGKPVASI EKLAVWGNHS PTMYPDFRFA TAEGESLLKL INDDVWNRDT FIPTVGKRGA AIIEARGLSS AASAANAAID HVRDWVLGTN GKWVTMGIPS DGSYGIPEDI IYGVPVICEN GEYKRVEGLE IDAFSREKMD GTLAELLEER DGVAHLLK
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggcta agcccgcaaa gcgcgttgcc gtcaccggcg ccgcaggtca gatcgcttac tcgctgctgt ttcgcatcgc gaacggcgac ctgctcggca aggatcagcc ggtcatcctg caactgctcg acctcccgca agcccaggcc gccgtcaaag gcgtcgtgat ggaactggac gattgcgcgt tcccgctgct cgcgggcgtc gtgatcaccg acgacccgaa ggtcgcgttc aaggacgccg acgtcgcgct gctggtgggc gcgcgtccgc gctcgaaggg catggagcgc aaggatctgc tgtcggcgaa cgccgagatc ttcacggtcc agggcgcggc gctgaacgaa gtcgcgagcc gcgacgtgaa ggtgctggtc gtcggcaacc cggcgaacac gaacgcgtac atcgcgatga agtcggcgcc ggatctgccg aagaagaact tcacggcgat gctgcgcctc gaccacaacc gcgcgctgtc gcagctcgcc gcgaagtcgg gcaagccggt cgcgtcgatc gagaagctcg ccgtgtgggg caaccactcg ccgacgatgt accccgactt ccgcttcgcg accgccgagg gcgaatcgct gctgaagctg atcaacgacg acgtgtggaa ccgcgacacg ttcatcccga ctgtcggcaa gcgcggcgcg gcgatcatcg aagcgcgcgg cctgtcgtcg gcggcgtcgg cggccaacgc ggcgatcgac cacgtgcgcg actgggtgct cggcacgaac ggcaagtggg tcacgatggg catcccgtcg gacggctcgt acggcatccc cgaggacatc atctacggcg tgccggtgat ttgcgaaaac ggcgagtaca agcgcgtcga gggcctggaa atcgacgcgt tctcgcgcga gaagatggac ggcacgctcg ccgagctgct cgaggagcgc gacggcgtcg cccatctgct gaagtaataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAKPAKRVA VTGAAGQIAY SLLFRIANGD LLGKDQPVIL QLLDLPQAQA AVKGVVMELD DCAFPLLAGV VITDDPKVAF KDADVALLVG ARPRSKGMER KDLLSANAEI FTVQGAALNE VASRDVKVLV VGNPANTNAY IAMKSAPDLP KKNFTAMLRL DHNRALSQLA AKSGKPVASI EKLAVWGNHS PTMYPDFRFA TAEGESLLKL INDDVWNRDT FIPTVGKRGA AIIEARGLSS AASAANAAID HVRDWVLGTN GKWVTMGIPS DGSYGIPEDI IYGVPVICEN GEYKRVEGLE IDAFSREKMD GTLAELLEER DGVAHLLK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggctaagccc gcaaagcgcg ttgccgtcac cggcgccgca ggtcagatcg cttactcgct gctgtttcgc atcgcgaacg gcgacctgct cggcaaggat cagccggtca tcctgcaact gctcgacctc ccgcaagccc aggccgccgt caaaggcgtc gtgatggaac tggacgattg cgcgttcccg ctgctcgcgg gcgtcgtgat caccgacgac ccgaaggtcg cgttcaagga cgccgacgtc gcgctgctgg tgggcgcgcg tccgcgctcg aagggcatgg agcgcaagga tctgctgtcg gcgaacgccg agatcttcac ggtccagggc gcggcgctga acgaagtcgc gagccgcgac gtgaaggtgc tggtcgtcgg caacccggcg aacacgaacg cgtacatcgc gatgaagtcg gcgccggatc tgccgaagaa gaacttcacg gcgatgctgc gcctcgacca caaccgcgcg ctgtcgcagc tcgccgcgaa gtcgggcaag ccggtcgcgt cgatcgagaa gctcgccgtg tggggcaacc actcgccgac gatgtacccc gacttccgct tcgcgaccgc cgagggcgaa tcgctgctga agctgatcaa cgacgacgtg tggaaccgcg acacgttcat cccgactgtc ggcaagcgcg gcgcggcgat catcgaagcg cgcggcctgt cgtcggcggc gtcggcggcc aacgcggcga tcgaccacgt gcgcgactgg gtgctcggca cgaacggcaa gtgggtcacg atgggcatcc cgtcggacgg ctcgtacggc atccccgagg acatcatcta cggcgtgccg gtgatttgcg aaaacggcga gtacaagcgc gtcgagggcc tggaaatcga cgcgttctcg cgcgagaaga tggacggcac gctcgccgag ctgctcgagg agcgcgacgg cgtcgcccat ctgctgaagt aaaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa
Details for BupsA.00005.a.A1.PW25021
| PURIFICATION DATe: | 3/4/2008 |
| CONCENTRATION: | 34.5mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 81 |
| VIAL VOLUME: | data unavailable |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAKPAKRVA VTGAAGQIAY SLLFRIANGD LLGKDQPVIL QLLDLPQAQA AVKGVVMELD DCAFPLLAGV VITDDPKVAF KDADVALLVG ARPRSKGMER KDLLSANAEI FTVQGAALNE VASRDVKVLV VGNPANTNAY IAMKSAPDLP KKNFTAMLRL DHNRALSQLA AKSGKPVASI EKLAVWGNHS PTMYPDFRFA TAEGESLLKL INDDVWNRDT FIPTVGKRGA AIIEARGLSS AASAANAAID HVRDWVLGTN GKWVTMGIPS DGSYGIPEDI IYGVPVICEN GEYKRVEGLE IDAFSREKMD GTLAELLEER DGVAHLLK
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggcta agcccgcaaa gcgcgttgcc gtcaccggcg ccgcaggtca gatcgcttac tcgctgctgt ttcgcatcgc gaacggcgac ctgctcggca aggatcagcc ggtcatcctg caactgctcg acctcccgca agcccaggcc gccgtcaaag gcgtcgtgat ggaactggac gattgcgcgt tcccgctgct cgcgggcgtc gtgatcaccg acgacccgaa ggtcgcgttc aaggacgccg acgtcgcgct gctggtgggc gcgcgtccgc gctcgaaggg catggagcgc aaggatctgc tgtcggcgaa cgccgagatc ttcacggtcc agggcgcggc gctgaacgaa gtcgcgagcc gcgacgtgaa ggtgctggtc gtcggcaacc cggcgaacac gaacgcgtac atcgcgatga agtcggcgcc ggatctgccg aagaagaact tcacggcgat gctgcgcctc gaccacaacc gcgcgctgtc gcagctcgcc gcgaagtcgg gcaagccggt cgcgtcgatc gagaagctcg ccgtgtgggg caaccactcg ccgacgatgt accccgactt ccgcttcgcg accgccgagg gcgaatcgct gctgaagctg atcaacgacg acgtgtggaa ccgcgacacg ttcatcccga ctgtcggcaa gcgcggcgcg gcgatcatcg aagcgcgcgg cctgtcgtcg gcggcgtcgg cggccaacgc ggcgatcgac cacgtgcgcg actgggtgct cggcacgaac ggcaagtggg tcacgatggg catcccgtcg gacggctcgt acggcatccc cgaggacatc atctacggcg tgccggtgat ttgcgaaaac ggcgagtaca agcgcgtcga gggcctggaa atcgacgcgt tctcgcgcga gaagatggac ggcacgctcg ccgagctgct cgaggagcgc gacggcgtcg cccatctgct gaagtaataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAKPAKRVA VTGAAGQIAY SLLFRIANGD LLGKDQPVIL QLLDLPQAQA AVKGVVMELD DCAFPLLAGV VITDDPKVAF KDADVALLVG ARPRSKGMER KDLLSANAEI FTVQGAALNE VASRDVKVLV VGNPANTNAY IAMKSAPDLP KKNFTAMLRL DHNRALSQLA AKSGKPVASI EKLAVWGNHS PTMYPDFRFA TAEGESLLKL INDDVWNRDT FIPTVGKRGA AIIEARGLSS AASAANAAID HVRDWVLGTN GKWVTMGIPS DGSYGIPEDI IYGVPVICEN GEYKRVEGLE IDAFSREKMD GTLAELLEER DGVAHLLK
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCTAAGCCC GCAAAGCGCG TTGCCGTCAC CGGCGCCGCA GGTCAGATCG CTTACTCGCT GCTGTTTCGC ATCGCGAACG GCGACCTGCT CGGCAAGGAT CAGCCGGTCA TCCTGCAACT GCTCGACCTC CCGCAAGCCC AGGCCGCCGT CAAAGGCGTC GTGATGGAAC TGGACGATTG CGCGTTCCCG CTGCTCGCGG GCGTCGTGAT CACCGACGAC CCGAAGGTCG CGTTCAAGGA CGCCGACGTC GCGCTGCTGG TGGGCGCGCG TCCGCGCTCG AAGGGCATGG AGCGCAAGGA TCTGCTGTCG GCGAACGCCG AGATCTTCAC GGTCCAGGGC GCGGCGCTGA ACGAAGTCGC GAGCCGCGAC GTGAAGGTGC TGGTCGTCGG CAACCCGGCG AACACGAACG CGTACATCGC GATGAAGTCG GCGCCGGATC TGCCGAAGAA GAACTTCACG GCGATGCTGC GCCTCGACCA CAACCGCGCG CTGTCGCAGC TCGCCGCGAA GTCGGGCAAG CCGGTCGCGT CGATCGAGAA GCTCGCCGTG TGGGGCAACC ACTCGCCGAC GATGTACCCC GACTTCCGCT TCGCGACCGC CGAGGGCGAA TCGCTGCTGA AGCTGATCAA CGACGACGTG TGGAACCGCG ACACGTTCAT CCCGACTGTC GGCAAGCGCG GCGCGGCGAT CATCGAAGCG CGCGGCCTGT CGTCGGCGGC GTCGGCGGCC AACGCGGCGA TCGACCACGT GCGCGACTGG GTGCTCGGCA CGAACGGCAA GTGGGTCACG ATGGGCATCC CGTCGGACGG CTCGTACGGC ATCCCCGAGG ACATCATCTA CGGCGTGCCG GTGATTTGCG AAAACGGCGA GTACAAGCGC GTCGAGGGCC TGGAAATCGA CGCGTTCTCG CGCGAGAAGA TGGACGGCAC GCTCGCCGAG CTGCTCGAGG AGCGCGACGG CGTCGCCCAT CTGCTGAAGT AAAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA