| CENTER ID: | BupsA.00003.a |
| ORGANISM: | Burkholderia pseudomallei 1710b |
| ASSOCIATED DISEASE: | Melioidosis |
| CURRENT STATUS: | purified |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIB |
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BupsA.00003.a.A1.GE24981 | Full length( BupsA.00003.a ) | 1 | 87 | ||
| BupsA.00003.a.A2.GE27905 | Created from Decode construct for feature BupsA.00003.a | 1 | 72 | ||
| BupsA.00003.a.A4.GE27907 | Created from Decode construct for feature BupsA.00003.a | 1 | 79 | ||
| BupsA.00003.a.A6.GE27909 | Created from Decode construct for feature BupsA.00003.a | 1 | 85 |
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BupsA.00003.a.D11.PD00022 | Full length( BupsA.00003.a ) | 1 | 87 | ||
| BupsA.00003.a.A1.PS00088 | Full length( BupsA.00003.a ) | 1 | 87 | ||
| BupsA.00003.a.A4.PS00466 | Created from Decode construct for feature BupsA.00003.a | 1 | 79 | ||
| BupsA.00003.a.A4.PS00467 | Created from Decode construct for feature BupsA.00003.a | 1 | 79 | ||
| BupsA.00003.a.A4.PS00468 | Created from Decode construct for feature BupsA.00003.a | 1 | 79 | ||
| BupsA.00003.a.A6.PS00477 | Created from Decode construct for feature BupsA.00003.a | 1 | 85 |
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_127361 |
| PATRIC ID: | fig|320372.6.peg.3665 |
| RefSeq: | YP_334894 |
| UniProt: | Q3JNF9 |
| HARVESTED ON: | 2/12/2008 |
| SEQUENCED ON: | 6/16/2009 |
| EXPECTED MW: | 11kDa |
| OBSERVED MW: | 12kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
| HARVESTED ON: | 11/24/2009 |
| SEQUENCED ON: | 12/3/2009 |
| EXPECTED MW: | 9kDa |
| OBSERVED MW: | 9kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Few (1-10) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
| HARVESTED ON: | 11/24/2009 |
| SEQUENCED ON: | 12/3/2009 |
| EXPECTED MW: | 10kDa |
| OBSERVED MW: | 10kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
| HARVESTED ON: | 11/24/2009 |
| SEQUENCED ON: | 12/3/2009 |
| EXPECTED MW: | 11kDa |
| OBSERVED MW: | 11kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
| PURIFICATION DATe: | 7/1/2008 |
| CONCENTRATION: | 5.7mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20mM Tris-HCl, pH 8.0, 200mM NaCl, 1% glycerol, 1mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 7 |
| VIAL VOLUME: | 50µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
| PURIFICATION DATe: | 10/15/2008 |
| CONCENTRATION: | 8mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 1 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
| PURIFICATION DATe: | 1/28/2010 |
| CONCENTRATION: | 3.8mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 6 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
| PURIFICATION DATe: | 1/28/2010 |
| CONCENTRATION: | 3.7mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 4 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
| PURIFICATION DATe: | 1/28/2010 |
| CONCENTRATION: | 5.8mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 6 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
| PURIFICATION DATe: | 2/11/2010 |
| CONCENTRATION: | 5mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 6 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |