SSGCID: Target Page

BuphA.18695.a

Autoinducer synthesis protein

CENTER ID:	BuphA.18695.a
ORGANISM:	Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuphA.18695.a.B1.GE37743	Full length(BuphA.18695.a)		1	195

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|391038.7.peg.4691
RefSeq:	WP_012403724.1
RefSeq:	YP_001860597.1
UniProt:	B2JQL0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MHTAIRIGTR QEFDNNHINE MYRLRARVFR DRMGWDIPTI AGMEIDGYDA LGPYYMLIQD GDRRVRGCWR LMPTEGPNML KDTFPQLLDG REAPLGRHIW ELSRFAIETD GAQTFGFAEL TMHAIHELVT FADRMGITRY VTVTTTAIER MLRRAGIEVT RLGPPVKIGV ERTIALDITV DAIRAAVCKP MPVAA

NT Sequence

ATGCATACGG CGATTCGGAT TGGCACACGG CAAGAGTTCG ACAACAACCA CATCAACGAG ATGTATCGAC TTCGCGCCCG GGTCTTCCGG GACCGGATGG GATGGGACAT CCCGACCATC GCGGGCATGG AGATCGACGG CTACGACGCC CTCGGGCCGT ACTACATGCT CATTCAGGAC GGCGATCGGC GGGTGCGCGG CTGCTGGCGG CTGATGCCGA CGGAAGGTCC GAACATGCTG AAGGACACTT TCCCGCAACT ACTCGACGGC CGGGAGGCGC CACTCGGGCG ACATATTTGG GAATTGAGCC GCTTCGCTAT CGAAACGGAT GGCGCGCAAA CGTTTGGCTT TGCAGAGTTG ACGATGCATG CGATCCACGA ACTCGTGACT TTCGCAGACC GCATGGGCAT TACGCGCTAC GTGACAGTCA CGACGACAGC GATCGAGCGG ATGCTGCGGC GCGCTGGCAT CGAGGTGACA CGGCTGGGGC CGCCCGTCAA GATCGGCGTC GAACGGACCA TCGCGCTCGA CATCACCGTG GATGCGATCC GCGCGGCGGT GTGCAAGCCG ATGCCCGTGG CGGCC

Details for BuphA.18695.a.B1.GE37743

HARVESTED ON:	2/5/2014
SEQUENCED ON:	2/6/2014
EXPECTED MW:	23kDa
OBSERVED MW:	23kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMH TAIRIGTRQE FDNNHINEMY RLRARVFRDR MGWDIPTIAG MEIDGYDALG PYYMLIQDGD RRVRGCWRLM PTEGPNMLKD TFPQLLDGRE APLGRHIWEL SRFAIETDGA QTFGFAELTM HAIHELVTFA DRMGITRYVT VTTTAIERML RRAGIEVTRL GPPVKIGVER TIALDITVDA IRAAVCKPMP VAA

Validated NT Sequence

atcttactca cttaggccgc cacgggcatc ggcttgcaca ccgccgcgcg gatcgcatcc acggtgatgt cgagcgcgat ggtccgttcg acgccgatct tgacgggcgg ccccagccgt gtcacctcga tgccagcgcg ccgcagcatc cgctcgatcg ctgtcgtcgt gactgtcacg tagcgcgtaa tgcccatgcg gtctgcgaaa gtcacgagtt cgtggatcgc atgcatcgtc aactctgcaa agccaaacgt ttgcgcgcca tccgtttcga tagcgaagcg gctcaattcc caaatatgtc gcccgagtgg cgcctcccgg ccgtcgagta gttgcgggaa agtgtccttc agcatgttcg gaccttccgt cggcatcagc cgccagcagc cgcgcacccg ccgatcgccg tcctgaatga gcatgtagta cggcccgagg gcgtcgtagc cgtcgatctc catgcccgcg atggtcggga tgtcccatcc catccggtcc cggaagaccc gggcgcgaag tcgatacatc tcgttgatgt ggttgttgtc gaactcttgc cgtgtgccaa tccgaatcgc cgtatgcata tggtggtggt ggtggtgagc cat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcata cggcgattcg gattggcaca cggcaagagt tcgacaacaa ccacatcaac gagatgtatc gacttcgcgc ccgggtcttc cgggaccgga tgggatggga catcccgacc atcgcgggca tggagatcga cggctacgac gccctcgggc cgtactacat gctcattcag gacggcgatc ggcgggtgcg cggctgctgg cggctgatgc cgacggaagg tccgaacatg ctgaaggaca ctttcccgca actactcgac ggccgggagg cgccactcgg gcgacatatt tgggaattga gccgcttcgc tatcgaaacg gatggcgcgc aaacgtttgg ctttgcagag ttgacgatgc atgcgatcca cgaactcgtg actttcgcag accgcatggg cattacgcgc tacgtgacag tcacgacgac agcgatcgag cggatgctgc ggcgcgctgg catcgaggtg acacggctgg ggccgcccgt caagatcggc gtcgaacgga ccatcgcgct cgacatcacc gtggatgcga tccgcgcggc ggtgtgcaag ccgatgcccg tggcggcctg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat