SSGCID: Target Page

BuphA.17267.a

SurA domain protein

CENTER ID:	BuphA.17267.a
ORGANISM:	Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuphA.17267.a.A2.GE32185	Mature protein( BuphA.17267.a )		30	463

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuphA.17267.a.A2.PW32103	Mature protein( BuphA.17267.a )		30	463

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|391038.7.peg.412
RefSeq:	YP_001856636.1
UniProt:	B2JCX1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAIMKKLRLA TFATGFAAAA SILLAGPAHA QALNGSGSAQ TVDTIAAVVN NGVITQRELD MRVGLITKRL NQQHAPIPPA DQLRQQVLNQ MVLERIQLQK AREDGITVDD ATVQRTLERL AQANNLQLDM YRARIEAQGV PWTTFMGDAK TELILSRLRE KEVDSKVTVS DAEVANYIAS QRGPTAGLAS DLHLEHIFLK APLNASQTDI EAAQAKANAL LQEALKGDNF GKLAKSNSQA PDASKGGDLG FQAPSRLPAE FVTAAATLRP GQVNPSVIRT NDGFEIVRLV ERRSGQGSAS ADSTKLVQTH VRHILLRVGD GMSEPQARQK LLEIRQEIEA GQGDFDKFAR TYSQDGSASQ GGDLGWISPG ETVPEFERAM NSLQDNQISQ PVRSEYGYHL IQVLGRREAE GSVSQQMDLA RQAIGQRKAE QAYADWLREL RDTAYVDYKV GPVTGTSADN TAQ

NT Sequence

GTGGCAATCA TGAAAAAGCT TCGCCTGGCA ACGTTCGCGA CCGGCTTCGC TGCGGCCGCT TCCATTCTGC TGGCCGGACC CGCGCACGCG CAGGCACTGA ACGGCAGCGG CAGTGCCCAA ACAGTCGATA CGATCGCGGC TGTCGTCAAT AACGGCGTGA TTACGCAGCG CGAGCTGGAC ATGCGCGTTG GCCTGATCAC GAAGCGGTTG AATCAGCAGC ACGCGCCGAT TCCGCCCGCC GACCAGTTGC GCCAGCAGGT GCTCAACCAG ATGGTGCTTG AGCGCATCCA GTTGCAAAAG GCGCGCGAAG ACGGCATCAC CGTCGACGAC GCGACGGTGC AGCGCACACT CGAACGTCTC GCCCAGGCGA ACAACTTGCA GCTCGACATG TACCGCGCAC GTATCGAGGC GCAAGGCGTG CCCTGGACGA CCTTCATGGG CGACGCGAAG ACGGAGCTGA TCCTGTCGCG TCTGCGCGAA AAGGAAGTGG ACAGCAAGGT CACGGTTTCG GACGCGGAAG TGGCGAACTA CATCGCCAGC CAGCGCGGCC CGACGGCAGG CCTCGCGAGC GATCTGCACC TCGAGCACAT TTTCCTGAAA GCGCCGCTCA ATGCGTCCCA GACGGATATC GAAGCAGCCC AGGCCAAGGC GAACGCGCTG CTTCAGGAAG CCTTGAAGGG CGATAACTTC GGCAAGCTCG CGAAGTCGAA TTCGCAGGCT CCCGATGCGT CGAAGGGCGG CGATCTGGGC TTTCAGGCAC CGTCCAGGCT ACCGGCTGAA TTCGTGACGG CGGCTGCGAC GCTGCGTCCG GGCCAGGTCA ACCCGTCCGT GATCCGCACC AACGACGGCT TCGAGATTGT GCGTCTCGTC GAGCGCCGCT CGGGTCAGGG CTCGGCTTCC GCCGACTCGA CGAAGCTCGT GCAGACGCAC GTGCGCCACA TCTTGCTGCG TGTCGGTGAC GGCATGTCGG AGCCGCAAGC ACGCCAGAAG CTGTTGGAAA TCCGCCAGGA AATCGAAGCG GGCCAAGGCG ACTTCGACAA GTTCGCGCGC ACGTATTCGC AGGACGGCTC GGCGTCGCAG GGTGGCGATC TGGGCTGGAT CAGCCCGGGC GAAACGGTCC CGGAGTTCGA GCGGGCGATG AATTCGCTGC AGGACAACCA GATCAGCCAG CCTGTGCGAA GCGAGTACGG CTACCACCTG ATCCAGGTGC TCGGCCGCCG GGAAGCGGAG GGCTCGGTCT CTCAGCAGAT GGATCTGGCG CGTCAGGCGA TCGGCCAGCG CAAGGCGGAA CAGGCCTATG CCGACTGGCT GCGTGAACTG CGTGACACCG CATATGTCGA TTACAAGGTA GGCCCGGTCA CGGGCACATC CGCGGACAAC ACCGCGCAA

Details for BuphA.17267.a.A2.GE32185

HARVESTED ON:	4/26/2011
SEQUENCED ON:	5/2/2011
EXPECTED MW:	49kDa
OBSERVED MW:	49kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	93

Validated AA Sequence

MAQALNGSGS AQTVDTIAAV VNNGVITQRE LDMRVGLITK RLNQQHAPIP PADQLRQQVL NQMVLERIQL QKAREDGITV DDATVQRTLE RLAQANNLQL DMYRARIEAQ GVPWTTFMGD AKTELILSRL REKEVDSKVT VSDAEVANYI ASQRGPTAGL ASDLHLEHIF LKAPLNASQT DIEAAQAKAN ALLQEALKGD NFGKLAKSNS QAPDASKGGD LGFQAPSRLP AEFVTAAATL RPGQVNPSVI RTNDGFEIVR LVERRSGQGS ASADSTKLVQ THVRHILLRV GDGMSEPQAR QKLLEIRQEI EAGQGDFDKF ARTYSQDGSA SQGGDLGWIS PGETVPEFER AMNSLQDNQI SQPVRSEYGY HLIQVLGRRE AEGSVSQQMD LARQAIGQRK AEQAYADWLR ELRDTAYVDY KVGPVTGT

Validated NT Sequence

atgtgcccgt gaccgggcct accttgtaat cgacatatgc ggtgtcacgc agttcacgca gccagtcggc ataggcctgt tccgccttgc gctggccgat cgcctgacgc gccagatcca tctgctgaga gaccgagccc tccgcttccc ggcggccgag cacctggatc aggtggtagc cgtactcgct tcgcacaggc tggctgatct ggttgtcctg cagcgaattc atcgcccgct cgaactccgg gaccgtttcg cccgggctga tccagcccag atcgccaccc tgcgacgccg agccgtcctg cgaatacgtg cgcgcgaact tgtcgaagtc gccttggccc gcttcgattt cctggcggat ttccaacagc ttctggcgtg cttgcggctc cgacatgccg tcaccgacac gcagcaagat gtggcgcacg tgcgtctgca cgagcttcgt cgagtcggcg gaagccgagc cctgacccga gcggcgctcg acgagacgca caatctcgaa gccgtcgttg gtgcggatca cggacgggtt gacctggccc ggacgcagcg tcgcagccgc cgtcacgaat tcagccggta gcctggacgg tgcctgaaag cccagatcgc cgcccttcga cgcatcggga gcctgcgaat tcgacttcgc gagcttgccg aagttatcgc ccttcaaggc ttcctgaagc agcgcgttcg ccttggcctg ggctgcttcg atatccgtct gggacgcatt gagcggcgct ttcaggaaaa tgtgctcgag gtgcagatcg ctcgcgaggc ctgccgtcgg gccgcgctgg ctggcgatgt agttcgccac ttccgcgtcc gaaaccgtga ccttgctgtc cacttccttt tcgcgcagac gcgacaggat cagctccgtc ttcgcgtcgc ccatgaaggt cgtccagggc acgccttgcg cctcgatacg tgcgcggtac atgtcgagct gcaagttgtt cgcctgggcg agacgttcga gtgtgcgctg caccgtcgcg tcgtcgacgg tgatgccgtc ttcgcgcgcc ttttgcaact ggatgcgctc aagcaccatc tggttgagca cctgctggcg caactggtcg gcgggcggaa tcggcgcgtg ctgctgattc aaccgcttcg tgatcaggcc aacgcgcatg tccagctcgc gctgcgtaat cacgccgtta ttgacgacag ccgcgatcgt atcgactgtt tgggcactgc cgctgccgtt cagtgcctgc gccatcgaac caggaccctg ggtctgagct tccagggtac cc

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMAQALNGSG SAQTVDTIAA VVNNGVITQR ELDMRVGLIT KRLNQQHAPI PPADQLRQQV LNQMVLERIQ LQKAREDGIT VDDATVQRTL ERLAQANNLQ LDMYRARIEA QGVPWTTFMG DAKTELILSR LREKEVDSKV TVSDAEVANY IASQRGPTAG LASDLHLEHI FLKAPLNASQ TDIEAAQAKA NALLQEALKG DNFGKLAKSN SQAPDASKGG DLGFQAPSRL PAEFVTAAAT LRPGQVNPSV IRTNDGFEIV RLVERRSGQG SASADSTKLV QTHVRHILLR VGDGMSEPQA RQKLLEIRQE IEAGQGDFDK FARTYSQDGS ASQGGDLGWI SPGETVPEFE RAMNSLQDNQ ISQPVRSEYG YHLIQVLGRR EAEGSVSQQM DLARQAIGQR KAEQAYADWL RELRDTAYVD YKVGPVTGTS ADNTAQ

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcgcaggca ctgaacggca gcggcagtgc ccaaacagtc gatacgatcg cggctgtcgt caataacggc gtgattacgc agcgcgagct ggacatgcgc gttggcctga tcacgaagcg gttgaatcag cagcacgcgc cgattccgcc cgccgaccag ttgcgccagc aggtgctcaa ccagatggtg cttgagcgca tccagttgca aaaggcgcgc gaagacggca tcaccgtcga cgacgcgacg gtgcagcgca cactcgaacg tctcgcccag gcgaacaact tgcagctcga catgtaccgc gcacgtatcg aggcgcaagg cgtgccctgg acgaccttca tgggcgacgc gaagacggag ctgatcctgt cgcgtctgcg cgaaaaggaa gtggacagca aggtcacggt ttcggacgcg gaagtggcga actacatcgc cagccagcgc ggcccgacgg caggcctcgc gagcgatctg cacctcgagc acattttcct gaaagcgccg ctcaatgcgt cccagacgga tatcgaagca gcccaggcca aggcgaacgc gctgcttcag gaagccttga agggcgataa cttcggcaag ctcgcgaagt cgaattcgca ggctcccgat gcgtcgaagg gcggcgatct gggctttcag gcaccgtcca ggctaccggc tgaattcgtg acggcggctg cgacgctgcg tccgggccag gtcaacccgt ccgtgatccg caccaacgac ggcttcgaga ttgtgcgtct cgtcgagcgc cgctcgggtc agggctcggc ttccgccgac tcgacgaagc tcgtgcagac gcacgtgcgc cacatcttgc tgcgtgtcgg tgacggcatg tcggagccgc aagcacgcca gaagctgttg gaaatccgcc aggaaatcga agcgggccaa ggcgacttcg acaagttcgc gcgcacgtat tcgcaggacg gctcggcgtc gcagggtggc gatctgggct ggatcagccc gggcgaaacg gtcccggagt tcgagcgggc gatgaattcg ctgcaggaca accagatcag ccagcctgtg cgaagcgagt acggctacca cctgatccag gtgctcggcc gccgggaagc ggagggctcg gtctctcagc agatggatct ggcgcgtcag gcgatcggcc agcgcaaggc ggaacaggcc tatgccgact ggctgcgtga actgcgtgac accgcatatg tcgattacaa ggtaggcccg gtcacgggca catccgcgga caacaccgcg caataaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa

Details for BuphA.17267.a.A2.PW32103

PURIFICATION DATe:	7/8/2011
CONCENTRATION:	41.86mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	7
VIAL VOLUME:	100µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	93

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCGCAGGCA CTGAACGGCA GCGGCAGTGC CCAAACAGTC GATACGATCG CGGCTGTCGT CAATAACGGC GTGATTACGC AGCGCGAGCT GGACATGCGC GTTGGCCTGA TCACGAAGCG GTTGAATCAG CAGCACGCGC CGATTCCGCC CGCCGACCAG TTGCGCCAGC AGGTGCTCAA CCAGATGGTG CTTGAGCGCA TCCAGTTGCA AAAGGCGCGC GAAGACGGCA TCACCGTCGA CGACGCGACG GTGCAGCGCA CACTCGAACG TCTCGCCCAG GCGAACAACT TGCAGCTCGA CATGTACCGC GCACGTATCG AGGCGCAAGG CGTGCCCTGG ACGACCTTCA TGGGCGACGC GAAGACGGAG CTGATCCTGT CGCGTCTGCG CGAAAAGGAA GTGGACAGCA AGGTCACGGT TTCGGACGCG GAAGTGGCGA ACTACATCGC CAGCCAGCGC GGCCCGACGG CAGGCCTCGC GAGCGATCTG CACCTCGAGC ACATTTTCCT GAAAGCGCCG CTCAATGCGT CCCAGACGGA TATCGAAGCA GCCCAGGCCA AGGCGAACGC GCTGCTTCAG GAAGCCTTGA AGGGCGATAA CTTCGGCAAG CTCGCGAAGT CGAATTCGCA GGCTCCCGAT GCGTCGAAGG GCGGCGATCT GGGCTTTCAG GCACCGTCCA GGCTACCGGC TGAATTCGTG ACGGCGGCTG CGACGCTGCG TCCGGGCCAG GTCAACCCGT CCGTGATCCG CACCAACGAC GGCTTCGAGA TTGTGCGTCT CGTCGAGCGC CGCTCGGGTC AGGGCTCGGC TTCCGCCGAC TCGACGAAGC TCGTGCAGAC GCACGTGCGC CACATCTTGC TGCGTGTCGG TGACGGCATG TCGGAGCCGC AAGCACGCCA GAAGCTGTTG GAAATCCGCC AGGAAATCGA AGCGGGCCAA GGCGACTTCG ACAAGTTCGC GCGCACGTAT TCGCAGGACG GCTCGGCGTC GCAGGGTGGC GATCTGGGCT GGATCAGCCC GGGCGAAACG GTCCCGGAGT TCGAGCGGGC GATGAATTCG CTGCAGGACA ACCAGATCAG CCAGCCTGTG CGAAGCGAGT ACGGCTACCA CCTGATCCAG GTGCTCGGCC GCCGGGAAGC GGAGGGCTCG GTCTCTCAGC AGATGGATCT GGCGCGTCAG GCGATCGGCC AGCGCAAGGC GGAACAGGCC TATGCCGACT GGCTGCGTGA ACTGCGTGAC ACCGCATATG TCGATTACAA GGTAGGCCCG GTCACGGGCA CATCCGCGGA CAACACCGCG CAATAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA