BuphA.17266.b

PpiC-type peptidyl-prolyl cis-trans isomerase

CENTER ID: BuphA.17266.b
ORGANISM: Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuphA.17266.b.A2.GE32162 Mature protein( BuphA.17266.b ) 27 284
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuphA.17266.b.A2.PW32080 Mature protein( BuphA.17266.b ) 27 284

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|391038.7.peg.7629
RefSeq: YP_001863368.1
UniProt: B2JXG0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKFQHWTTFA AATALYLGLT APVPAQTASA PAASLPAGTV AIINGVSIPQ AQLDAAVQAT RQPDTPQLRQ ALKAQLITRE LFRQNAEKAG YGNKPEVQQV INATKVNAES QLYLKDNIHP DAVTDAQVKS RYDEIVASLG KDDYRPRIIA VADAMTAATV LGGLKAGKSF DVLARQYSIT PDKANGGELP WVSFKAPAAE GKTSGLPLPV AQALTQLPVG GVTPEPVIVG NARVIVKLDA KRPTQVPKFD QVKDTIRQQL QALALEKASA QFTAGLLKNA TIQQ
NT Sequence
ATGAAATTCC AACACTGGAC AACCTTTGCC GCAGCGACGG CACTGTATCT GGGTCTCACC GCGCCCGTGC CTGCGCAGAC GGCTTCGGCG CCGGCCGCGA GCCTCCCGGC AGGCACGGTC GCCATCATCA ACGGCGTGTC GATCCCCCAG GCGCAGCTCG ATGCGGCCGT GCAAGCCACG CGCCAGCCGG ACACGCCGCA ACTGCGCCAG GCGCTCAAGG CGCAACTGAT TACGCGCGAA CTGTTCCGGC AGAACGCCGA GAAGGCGGGC TATGGCAACA AGCCTGAGGT GCAGCAGGTC ATCAATGCGA CGAAGGTCAA CGCGGAGTCG CAGCTTTACC TGAAGGACAA CATCCATCCC GATGCTGTCA CTGATGCCCA GGTCAAGTCG CGTTATGACG AGATCGTTGC GTCGCTCGGC AAGGACGATT ACAGGCCGCG CATCATTGCC GTCGCTGACG CGATGACGGC TGCAACGGTG CTCGGTGGAC TGAAGGCGGG CAAGTCGTTC GATGTGCTCG CGCGCCAGTA CAGCATCACG CCGGATAAGG CAAACGGCGG CGAACTGCCG TGGGTGAGCT TCAAAGCTCC GGCAGCCGAG GGCAAGACCA GCGGACTGCC CCTGCCGGTT GCGCAGGCGC TCACTCAGTT GCCCGTAGGT GGCGTGACAC CCGAGCCGGT GATCGTCGGC AACGCGCGTG TGATCGTGAA GCTGGATGCG AAGCGGCCGA CGCAGGTGCC AAAATTCGAC CAGGTGAAAG ACACGATCCG GCAGCAGCTC CAGGCGCTGG CGCTGGAGAA GGCCAGTGCA CAATTCACGG CAGGTCTGCT GAAGAATGCC ACGATCCAGC AA
Details for BuphA.17266.b.A2.GE32162
HARVESTED ON: 4/26/2011
SEQUENCED ON: 5/2/2011
EXPECTED MW: 29kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 96
Validated AA Sequence
MGTLEAQTQG PGSMTASAPA ASLPAGTVAI INGVSIPQAQ LDAAVQATRQ PDTPQLRQAL KAQLITRELF RQNAEKAGYG NKPEVQQVIN ATKVNAESQL YLKDNIHPDA VTDAQVKSRY DEIVASLGKD DYRPRIIAVA DAMTAATVLG GLKAGKSFDV LARQYSITPD KANGGELPWV SFKAPAAEGK TSGLPLPVAQ ALTQLPVGGV TPEPVIVGNA RVIVKLDAKR PTQVPKFDQV KDTIRQQLQA LALEKASAQF TAGLLKNAT
Validated NT Sequence
atcgtggcat tcttcagcag acctgccgtg aattgtgcac tggccttctc cagcgccagc gcctggagct gctgccggat cgtgtctttc acctggtcga attttggcac ctgcgtcggc cgcttcgcat ccagcttcac gatcacacgc gcgttgccga cgatcaccgg ctcgggtgtc acgccaccta cgggcaactg agtgagcgcc tgcgcaaccg gcaggggcag tccgctggtc ttgccctcgg ctgccggagc tttgaagctc acccacggca gttcgccgcc gtttgcctta tccggcgtga tgctgtactg gcgcgcgagc acatcgaacg acttgcccgc cttcagtcca ccgagcaccg ttgcagccgt catcgcgtca gcgacggcaa tgatgcgcgg cctgtaatcg tccttgccga gcgacgcaac gatctcgtca taacgcgact tgacctgggc atcagtgaca gcatcgggat ggatgttgtc cttcaggtaa agctgcgact ccgcgttgac cttcgtcgca ttgatgacct gctgcacctc aggcttgttg ccatagcccg ccttctcggc gttctgccgg aacagttcgc gcgtaatcag ttgcgccttg agcgcctggc gcagttgcgg cgtgtccggc tggcgcgtgg cttgcacggc cgcatcgagc tgcgcctggg ggatcgacac gccgttgatg atggcgaccg tgcctgccgg gaggctcgcg gccggcgccg aagccgtcat cgaaccagga ccctgggtct gagcttccag ggtacccata tgatggtgat ggtgatgagc cangg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTASAPAAS LPAGTVAIIN GVSIPQAQLD AAVQATRQPD TPQLRQALKA QLITRELFRQ NAEKAGYGNK PEVQQVINAT KVNAESQLYL KDNIHPDAVT DAQVKSRYDE IVASLGKDDY RPRIIAVADA MTAATVLGGL KAGKSFDVLA RQYSITPDKA NGGELPWVSF KAPAAEGKTS GLPLPVAQAL TQLPVGGVTP EPVIVGNARV IVKLDAKRPT QVPKFDQVKD TIRQQLQALA LEKASAQFTA GLLKNATIQQ
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacggcttcg gcgccggccg cgagcctccc ggcaggcacg gtcgccatca tcaacggcgt gtcgatcccc caggcgcagc tcgatgcggc cgtgcaagcc acgcgccagc cggacacgcc gcaactgcgc caggcgctca aggcgcaact gattacgcgc gaactgttcc ggcagaacgc cgagaaggcg ggctatggca acaagcctga ggtgcagcag gtcatcaatg cgacgaaggt caacgcggag tcgcagcttt acctgaagga caacatccat cccgatgctg tcactgatgc ccaggtcaag tcgcgttatg acgagatcgt tgcgtcgctc ggcaaggacg attacaggcc gcgcatcatt gccgtcgctg acgcgatgac ggctgcaacg gtgctcggtg gactgaaggc gggcaagtcg ttcgatgtgc tcgcgcgcca gtacagcatc acgccggata aggcaaacgg cggcgaactg ccgtgggtga gcttcaaagc tccggcagcc gagggcaaga ccagcggact gcccctgccg gttgcgcagg cgctcactca gttgcccgta ggtggcgtga cacccgagcc ggtgatcgtc ggcaacgcgc gtgtgatcgt gaagctggat gcgaagcggc cgacgcaggt gccaaaattc gaccaggtga aagacacgat ccggcagcag ctccaggcgc tggcgctgga gaaggccagt gcacaattca cggcaggtct gctgaagaat gccacgatcc agcaataaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for BuphA.17266.b.A2.PW32080
PURIFICATION DATe: 6/10/2011
CONCENTRATION: 30.29mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 11
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 96
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMTASAPA ASLPAGTVAI INGVSIPQAQ LDAAVQATRQ PDTPQLRQAL KAQLITRELF RQNAEKAGYG NKPEVQQVIN ATKVNAESQL YLKDNIHPDA VTDAQVKSRY DEIVASLGKD DYRPRIIAVA DAMTAATVLG GLKAGKSFDV LARQYSITPD KANGGELPWV SFKAPAAEGK TSGLPLPVAQ ALTQLPVGGV TPEPVIVGNA RVIVKLDAKR PTQVPKFDQV KDTIRQQLQA LALEKASAQF TAGLLKNAT
Validated NT Sequence
atcgtggcat tcttcagcag acctgccgtg aattgtgcac tggccttctc cagcgccagc gcctggagct gctgccggat cgtgtctttc acctggtcga attttggcac ctgcgtcggc cgcttcgcat ccagcttcac gatcacacgc gcgttgccga cgatcaccgg ctcgggtgtc acgccaccta cgggcaactg agtgagcgcc tgcgcaaccg gcaggggcag tccgctggtc ttgccctcgg ctgccggagc tttgaagctc acccacggca gttcgccgcc gtttgcctta tccggcgtga tgctgtactg gcgcgcgagc acatcgaacg acttgcccgc cttcagtcca ccgagcaccg ttgcagccgt catcgcgtca gcgacggcaa tgatgcgcgg cctgtaatcg tccttgccga gcgacgcaac gatctcgtca taacgcgact tgacctgggc atcagtgaca gcatcgggat ggatgttgtc cttcaggtaa agctgcgact ccgcgttgac cttcgtcgca ttgatgacct gctgcacctc aggcttgttg ccatagcccg ccttctcggc gttctgccgg aacagttcgc gcgtaatcag ttgcgccttg agcgcctggc gcagttgcgg cgtgtccggc tggcgcgtgg cttgcacggc cgcatcgagc tgcgcctggg ggatcgacac gccgttgatg atggcgaccg tgcctgccgg gaggctcgcg gccggcgccg aagccgtcat cgaaccagga ccctgggtct gagcttccag ggtacccata tgatggtgat ggtgatgagc cangg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTASAPAAS LPAGTVAIIN GVSIPQAQLD AAVQATRQPD TPQLRQALKA QLITRELFRQ NAEKAGYGNK PEVQQVINAT KVNAESQLYL KDNIHPDAVT DAQVKSRYDE IVASLGKDDY RPRIIAVADA MTAATVLGGL KAGKSFDVLA RQYSITPDKA NGGELPWVSF KAPAAEGKTS GLPLPVAQAL TQLPVGGVTP EPVIVGNARV IVKLDAKRPT QVPKFDQVKD TIRQQLQALA LEKASAQFTA GLLKNATIQQ
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACGGCTTCG GCGCCGGCCG CGAGCCTCCC GGCAGGCACG GTCGCCATCA TCAACGGCGT GTCGATCCCC CAGGCGCAGC TCGATGCGGC CGTGCAAGCC ACGCGCCAGC CGGACACGCC GCAACTGCGC CAGGCGCTCA AGGCGCAACT GATTACGCGC GAACTGTTCC GGCAGAACGC CGAGAAGGCG GGCTATGGCA ACAAGCCTGA GGTGCAGCAG GTCATCAATG CGACGAAGGT CAACGCGGAG TCGCAGCTTT ACCTGAAGGA CAACATCCAT CCCGATGCTG TCACTGATGC CCAGGTCAAG TCGCGTTATG ACGAGATCGT TGCGTCGCTC GGCAAGGACG ATTACAGGCC GCGCATCATT GCCGTCGCTG ACGCGATGAC GGCTGCAACG GTGCTCGGTG GACTGAAGGC GGGCAAGTCG TTCGATGTGC TCGCGCGCCA GTACAGCATC ACGCCGGATA AGGCAAACGG CGGCGAACTG CCGTGGGTGA GCTTCAAAGC TCCGGCAGCC GAGGGCAAGA CCAGCGGACT GCCCCTGCCG GTTGCGCAGG CGCTCACTCA GTTGCCCGTA GGTGGCGTGA CACCCGAGCC GGTGATCGTC GGCAACGCGC GTGTGATCGT GAAGCTGGAT GCGAAGCGGC CGACGCAGGT GCCAAAATTC GACCAGGTGA AAGACACGAT CCGGCAGCAG CTCCAGGCGC TGGCGCTGGA GAAGGCCAGT GCACAATTCA CGGCAGGTCT GCTGAAGAAT GCCACGATCC AGCAATAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA