BuphA.17231.a

4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (IspG/GcpE)

CENTER ID: BuphA.17231.a
ORGANISM: Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuphA.17231.a.A1.GE32186 Full length( BuphA.17231.a ) 1 437
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuphA.17231.a.A1.PW32104 Full length( BuphA.17231.a ) 1 437

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|391038.7.peg.1511
RefSeq: YP_001857648.1
UniProt: B2JIV1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MHSDQVKSSS QIVSTVPVFG GHAPRRRSNA VDVRWGGQLV TIGGDAPVRV QSMTNTDTAD AIGTAIQIKE LAQAGSELVR ITVNTPEAAA AVPAIREQLD RMGVTVPLVG DFHYNGHLLL RDYPGCAESL SKYRINPGNV GQGAKRDTQF AQMIEAAARY DKPVRIGVNW GSLDQDLLAK MMDENAARAE PWEAQSVMYE ALIQSAIGSA ERAVELGLGR DKIILSCKVS GVQDLIAVYQ ELARRCDFAL HLGLTEAGMG SKGIVASTAA LSVLLQQGIG DTIRISLTPE PGASRTGEVI VGQEILQTMG LRSFAPMVIA CPGCGRTTST LFQELASQIQ SYLRTQMPLW RDTYPGVEKM HVAVMGCIVN GPGESKHANI GISLPGSGEN PAAPVFIDGV KVKTLRGENI AQEFQQIVSD YVERSYGPNA VRAEATN
NT Sequence
ATGCACTCCG ATCAAGTGAA ATCCAGCAGT CAGATTGTTT CGACCGTGCC GGTGTTCGGC GGTCATGCGC CGCGTCGCCG CTCGAATGCG GTCGACGTGC GTTGGGGCGG CCAACTCGTG ACGATCGGCG GCGACGCGCC CGTGCGCGTG CAGTCGATGA CCAACACGGA TACGGCCGAT GCAATCGGCA CCGCGATACA GATCAAGGAA CTCGCGCAGG CCGGTTCGGA GCTCGTGCGT ATCACGGTGA ATACGCCGGA AGCGGCGGCC GCCGTGCCCG CGATCCGCGA GCAGCTCGAC CGCATGGGCG TGACGGTGCC GCTGGTCGGT GACTTCCATT ACAACGGACA TTTGTTGCTG CGCGACTATC CGGGCTGCGC GGAATCGCTG TCGAAGTACC GCATCAACCC GGGCAACGTC GGTCAGGGCG CGAAGCGCGA CACGCAGTTC GCGCAGATGA TCGAAGCCGC AGCCAGGTAC GACAAGCCGG TGCGTATCGG CGTGAACTGG GGCAGCCTCG ATCAGGACCT GCTCGCGAAG ATGATGGACG AGAACGCCGC GCGCGCCGAA CCGTGGGAAG CGCAAAGCGT GATGTACGAA GCGCTGATCC AGTCGGCGAT CGGCTCGGCG GAACGTGCTG TCGAACTGGG TCTCGGCCGC GACAAGATCA TCCTGTCGTG CAAGGTGAGC GGCGTGCAGG ATCTGATCGC CGTCTACCAG GAACTCGCGC GCCGCTGCGA CTTCGCGCTG CACCTGGGCC TTACGGAAGC GGGCATGGGG TCGAAGGGCA TCGTCGCGTC GACGGCTGCA CTGTCCGTGC TGCTGCAGCA GGGCATCGGC GATACGATCC GTATTTCGCT GACGCCGGAA CCGGGCGCAT CGCGCACTGG CGAGGTGATC GTCGGCCAGG AAATCCTGCA GACGATGGGC CTGCGTTCGT TCGCGCCAAT GGTCATCGCG TGCCCGGGTT GCGGGCGCAC GACGAGCACG CTGTTCCAGG AGCTGGCGTC GCAGATTCAG AGTTATCTGC GCACCCAGAT GCCCCTGTGG CGCGATACGT ATCCGGGGGT CGAGAAGATG CACGTCGCCG TAATGGGCTG CATCGTCAAC GGGCCGGGCG AATCGAAGCA CGCGAACATC GGTATCAGCC TGCCCGGCTC GGGTGAGAAC CCGGCTGCGC CCGTCTTCAT CGACGGCGTG AAGGTCAAGA CGCTGCGCGG CGAAAACATC GCGCAGGAAT TCCAGCAGAT CGTGAGCGAC TACGTCGAGC GCAGCTATGG GCCCAACGCG GTCCGCGCCG AAGCGACGAA C
Details for BuphA.17231.a.A1.GE32186
HARVESTED ON: 4/26/2011
SEQUENCED ON: 5/2/2011
EXPECTED MW: 49kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 94
Validated AA Sequence
MHSDQVKSSS QIVSTVPVFG GHAPRRRSXX VDXXWGGQLV TIGGDAPVRV QSMTNTDTAD AIGTAIQIKE LAQAGSELVR ITVNTPEAAA AVPAIREQLD RMGVTVPLVG DFHYNGHLLL RDYPGCAESL SKYRINPGNV GQGAKRDTQF AQMIEAAARY DKPVRIGVNW GSLDQDLLAK MMDENAARAE PWEAQSVMYE ALIQSAIGSA ERAVELGLGR DKIILSCKVS GVQDLIAVYQ ELARRCDFAL HLGLTEAGMG SKGIVASTAA LSVLLQQGIG DTIRISLTPE PGASRTGEVI VGQEILQTMG LRSFAPMVIA CPGCGRTTST LFQELASQIQ SYLRTQMPLW RDTYPGVEKM HVAVMGCIVN GPGESKHANI GISLPGSGEN PAAPVFIDGV KVKTLRGENI AQEFQQIVSD YVERSYGPNA VRAEA
Validated NT Sequence
gtcgcttcgg cgcggaccgc gttgggccca tagctgcgct cgacgtagtc gctcacgatc tgctggaatt cctgcgcgat gttttcgccg cgcagcgtct tgaccttcac gccgtcgatg aagacgggcg cagccgggtt ctcacccgag ccgggcaggc tgataccgat gttcgcgtgc ttcgattcgc ccggcccgtt gacgatgcag cccattacgg cgacgtgcat cttctcgacc cccggatacg tatcgcgcca caggggcatc tgggtgcgca gataactctg aatctgcgac gccagctcct ggaacagcgt gctcgtcgtg cgcccgcaac ccgggcacgc gatgaccatt ggcgcgaacg aacgcaggcc catcgtctgc aggatttcct ggccgacgat cacctcgcca gtgcgcgatg cgcccggttc cggcgtcagc gaaatacgga tcgtatcgcc gatgccctgc tgcagcagca cggacagtgc agccgtcgac gcgacgatgc ccttcgaccc catgcccgct tccgtaaggc ccaggtgcag cgcgaagtcg cagcggcgcg cgagttcctg gtagacggcg atcagatcct gcacgccgct caccttgcac gacaggatga tcttgtcgcg gccgagaccc agttcgacag cacgttccgc cgagccgatc gccgactgga tcagcgcttc gtacatcacg ctttgcgctt cccacggttc ggcgcgcgcg gcgttctcgt ccatcatctt cgcgagcagg tcctgatcga ggctgcccca gttcacgccg atacgcaccg gcttgtcgta cctggctgcg gcttcgatca tctgcgcgaa ctgcgtgtcg cgcttcgcgc cctgaccgac gttgcccggg ttgatgcggt acttcgacag cgattccgcg cagcccggat agtcgcgcag caacaaatgt ccgttgtaat ggaagtcacc gaccagcggc accgtcacgc ccatgcggtc gagctgctcg cggatcgcgg gcacggcggc cgccgcttcc ggcgtattca ccgtgatacg cacgagctcc gaaccggcct gcgcgagttc cttgatctgt atcgcggtgc cgattgcatc ggccgtatcc gtgttggtca tcgactgcac gcgcacgggc gcgtcgccgc cgatcgtcac gagttggccg ccccancnca ngtcgacnnc atncgagcgg cgacgcggcg catgaccgcc gaacaccggc acggtcgaaa caatctgact gctggatttc acttgatcgg agtgcatcga accaggaccc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMHSDQVKSS SQIVSTVPVF GGHAPRRRSN AVDVRWGGQL VTIGGDAPVR VQSMTNTDTA DAIGTAIQIK ELAQAGSELV RITVNTPEAA AAVPAIREQL DRMGVTVPLV GDFHYNGHLL LRDYPGCAES LSKYRINPGN VGQGAKRDTQ FAQMIEAAAR YDKPVRIGVN WGSLDQDLLA KMMDENAARA EPWEAQSVMY EALIQSAIGS AERAVELGLG RDKIILSCKV SGVQDLIAVY QELARRCDFA LHLGLTEAGM GSKGIVASTA ALSVLLQQGI GDTIRISLTP EPGASRTGEV IVGQEILQTM GLRSFAPMVI ACPGCGRTTS TLFQELASQI QSYLRTQMPL WRDTYPGVEK MHVAVMGCIV NGPGESKHAN IGISLPGSGE NPAAPVFIDG VKVKTLRGEN IAQEFQQIVS DYVERSYGPN AVRAEATN
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcactccgat caagtgaaat ccagcagtca gattgtttcg accgtgccgg tgttcggcgg tcatgcgccg cgtcgccgct cgaatgcggt cgacgtgcgt tggggcggcc aactcgtgac gatcggcggc gacgcgcccg tgcgcgtgca gtcgatgacc aacacggata cggccgatgc aatcggcacc gcgatacaga tcaaggaact cgcgcaggcc ggttcggagc tcgtgcgtat cacggtgaat acgccggaag cggcggccgc cgtgcccgcg atccgcgagc agctcgaccg catgggcgtg acggtgccgc tggtcggtga cttccattac aacggacatt tgttgctgcg cgactatccg ggctgcgcgg aatcgctgtc gaagtaccgc atcaacccgg gcaacgtcgg tcagggcgcg aagcgcgaca cgcagttcgc gcagatgatc gaagccgcag ccaggtacga caagccggtg cgtatcggcg tgaactgggg cagcctcgat caggacctgc tcgcgaagat gatggacgag aacgccgcgc gcgccgaacc gtgggaagcg caaagcgtga tgtacgaagc gctgatccag tcggcgatcg gctcggcgga acgtgctgtc gaactgggtc tcggccgcga caagatcatc ctgtcgtgca aggtgagcgg cgtgcaggat ctgatcgccg tctaccagga actcgcgcgc cgctgcgact tcgcgctgca cctgggcctt acggaagcgg gcatggggtc gaagggcatc gtcgcgtcga cggctgcact gtccgtgctg ctgcagcagg gcatcggcga tacgatccgt atttcgctga cgccggaacc gggcgcatcg cgcactggcg aggtgatcgt cggccaggaa atcctgcaga cgatgggcct gcgttcgttc gcgccaatgg tcatcgcgtg cccgggttgc gggcgcacga cgagcacgct gttccaggag ctggcgtcgc agattcagag ttatctgcgc acccagatgc ccctgtggcg cgatacgtat ccgggggtcg agaagatgca cgtcgccgta atgggctgca tcgtcaacgg gccgggcgaa tcgaagcacg cgaacatcgg tatcagcctg cccggctcgg gtgagaaccc ggctgcgccc gtcttcatcg acggcgtgaa ggtcaagacg ctgcgcggcg aaaacatcgc gcaggaattc cagcagatcg tgagcgacta cgtcgagcgc agctatgggc ccaacgcggt ccgcgccgaa gcgacgaact aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for BuphA.17231.a.A1.PW32104
PURIFICATION DATe: 7/8/2011
CONCENTRATION: 45.26mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 100µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 94
Protocol Notes
notes unavailable
Validated AA Sequence
MHSDQVKSSS QIVSTVPVFG GHAPRRRSXX VDXXWGGQLV TIGGDAPVRV QSMTNTDTAD AIGTAIQIKE LAQAGSELVR ITVNTPEAAA AVPAIREQLD RMGVTVPLVG DFHYNGHLLL RDYPGCAESL SKYRINPGNV GQGAKRDTQF AQMIEAAARY DKPVRIGVNW GSLDQDLLAK MMDENAARAE PWEAQSVMYE ALIQSAIGSA ERAVELGLGR DKIILSCKVS GVQDLIAVYQ ELARRCDFAL HLGLTEAGMG SKGIVASTAA LSVLLQQGIG DTIRISLTPE PGASRTGEVI VGQEILQTMG LRSFAPMVIA CPGCGRTTST LFQELASQIQ SYLRTQMPLW RDTYPGVEKM HVAVMGCIVN GPGESKHANI GISLPGSGEN PAAPVFIDGV KVKTLRGENI AQEFQQIVSD YVERSYGPNA VRAEA
Validated NT Sequence
gtcgcttcgg cgcggaccgc gttgggccca tagctgcgct cgacgtagtc gctcacgatc tgctggaatt cctgcgcgat gttttcgccg cgcagcgtct tgaccttcac gccgtcgatg aagacgggcg cagccgggtt ctcacccgag ccgggcaggc tgataccgat gttcgcgtgc ttcgattcgc ccggcccgtt gacgatgcag cccattacgg cgacgtgcat cttctcgacc cccggatacg tatcgcgcca caggggcatc tgggtgcgca gataactctg aatctgcgac gccagctcct ggaacagcgt gctcgtcgtg cgcccgcaac ccgggcacgc gatgaccatt ggcgcgaacg aacgcaggcc catcgtctgc aggatttcct ggccgacgat cacctcgcca gtgcgcgatg cgcccggttc cggcgtcagc gaaatacgga tcgtatcgcc gatgccctgc tgcagcagca cggacagtgc agccgtcgac gcgacgatgc ccttcgaccc catgcccgct tccgtaaggc ccaggtgcag cgcgaagtcg cagcggcgcg cgagttcctg gtagacggcg atcagatcct gcacgccgct caccttgcac gacaggatga tcttgtcgcg gccgagaccc agttcgacag cacgttccgc cgagccgatc gccgactgga tcagcgcttc gtacatcacg ctttgcgctt cccacggttc ggcgcgcgcg gcgttctcgt ccatcatctt cgcgagcagg tcctgatcga ggctgcccca gttcacgccg atacgcaccg gcttgtcgta cctggctgcg gcttcgatca tctgcgcgaa ctgcgtgtcg cgcttcgcgc cctgaccgac gttgcccggg ttgatgcggt acttcgacag cgattccgcg cagcccggat agtcgcgcag caacaaatgt ccgttgtaat ggaagtcacc gaccagcggc accgtcacgc ccatgcggtc gagctgctcg cggatcgcgg gcacggcggc cgccgcttcc ggcgtattca ccgtgatacg cacgagctcc gaaccggcct gcgcgagttc cttgatctgt atcgcggtgc cgattgcatc ggccgtatcc gtgttggtca tcgactgcac gcgcacgggc gcgtcgccgc cgatcgtcac gagttggccg ccccancnca ngtcgacnnc atncgagcgg cgacgcggcg catgaccgcc gaacaccggc acggtcgaaa caatctgact gctggatttc acttgatcgg agtgcatcga accaggaccc
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMHSDQVKSS SQIVSTVPVF GGHAPRRRSN AVDVRWGGQL VTIGGDAPVR VQSMTNTDTA DAIGTAIQIK ELAQAGSELV RITVNTPEAA AAVPAIREQL DRMGVTVPLV GDFHYNGHLL LRDYPGCAES LSKYRINPGN VGQGAKRDTQ FAQMIEAAAR YDKPVRIGVN WGSLDQDLLA KMMDENAARA EPWEAQSVMY EALIQSAIGS AERAVELGLG RDKIILSCKV SGVQDLIAVY QELARRCDFA LHLGLTEAGM GSKGIVASTA ALSVLLQQGI GDTIRISLTP EPGASRTGEV IVGQEILQTM GLRSFAPMVI ACPGCGRTTS TLFQELASQI QSYLRTQMPL WRDTYPGVEK MHVAVMGCIV NGPGESKHAN IGISLPGSGE NPAAPVFIDG VKVKTLRGEN IAQEFQQIVS DYVERSYGPN AVRAEATN
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCACTCCGAT CAAGTGAAAT CCAGCAGTCA GATTGTTTCG ACCGTGCCGG TGTTCGGCGG TCATGCGCCG CGTCGCCGCT CGAATGCGGT CGACGTGCGT TGGGGCGGCC AACTCGTGAC GATCGGCGGC GACGCGCCCG TGCGCGTGCA GTCGATGACC AACACGGATA CGGCCGATGC AATCGGCACC GCGATACAGA TCAAGGAACT CGCGCAGGCC GGTTCGGAGC TCGTGCGTAT CACGGTGAAT ACGCCGGAAG CGGCGGCCGC CGTGCCCGCG ATCCGCGAGC AGCTCGACCG CATGGGCGTG ACGGTGCCGC TGGTCGGTGA CTTCCATTAC AACGGACATT TGTTGCTGCG CGACTATCCG GGCTGCGCGG AATCGCTGTC GAAGTACCGC ATCAACCCGG GCAACGTCGG TCAGGGCGCG AAGCGCGACA CGCAGTTCGC GCAGATGATC GAAGCCGCAG CCAGGTACGA CAAGCCGGTG CGTATCGGCG TGAACTGGGG CAGCCTCGAT CAGGACCTGC TCGCGAAGAT GATGGACGAG AACGCCGCGC GCGCCGAACC GTGGGAAGCG CAAAGCGTGA TGTACGAAGC GCTGATCCAG TCGGCGATCG GCTCGGCGGA ACGTGCTGTC GAACTGGGTC TCGGCCGCGA CAAGATCATC CTGTCGTGCA AGGTGAGCGG CGTGCAGGAT CTGATCGCCG TCTACCAGGA ACTCGCGCGC CGCTGCGACT TCGCGCTGCA CCTGGGCCTT ACGGAAGCGG GCATGGGGTC GAAGGGCATC GTCGCGTCGA CGGCTGCACT GTCCGTGCTG CTGCAGCAGG GCATCGGCGA TACGATCCGT ATTTCGCTGA CGCCGGAACC GGGCGCATCG CGCACTGGCG AGGTGATCGT CGGCCAGGAA ATCCTGCAGA CGATGGGCCT GCGTTCGTTC GCGCCAATGG TCATCGCGTG CCCGGGTTGC GGGCGCACGA CGAGCACGCT GTTCCAGGAG CTGGCGTCGC AGATTCAGAG TTATCTGCGC ACCCAGATGC CCCTGTGGCG CGATACGTAT CCGGGGGTCG AGAAGATGCA CGTCGCCGTA ATGGGCTGCA TCGTCAACGG GCCGGGCGAA TCGAAGCACG CGAACATCGG TATCAGCCTG CCCGGCTCGG GTGAGAACCC GGCTGCGCCC GTCTTCATCG ACGGCGTGAA GGTCAAGACG CTGCGCGGCG AAAACATCGC GCAGGAATTC CAGCAGATCG TGAGCGACTA CGTCGAGCGC AGCTATGGGC CCAACGCGGT CCGCGCCGAA GCGACGAACT AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA