BuphA.17230.b

Transketolase central region

CENTER ID: BuphA.17230.b
ORGANISM: Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuphA.17230.b.A1.GE32181 Full length( BuphA.17230.b ) 1 333
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|391038.7.peg.6194
RefSeq: YP_001861988.1
UniProt: B2JVG5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNSVIDKKPR LKTSAMIASI AGEGQITRSA PFGHALAELA RTKTNVIGMT ADLGKYTDLH IFAKAFPERY YQMGMAEQLL MGAAAGFAHE GAQPFVTTYA VFATRRAYDF IHQTIAEDDL DVKIVCALPG LTTGYGPSHQ AAEDLALMRA MPNMTVIDPC DALDIEQMVP AIAAHKGPVY ARLLRGNVPA VLDEYDYRFE LGKAKMLRDG ADVLIVSSGM MTMRALEAAK ALEADNVDVA VLHVPTIKPL DTESIIREAR RSGRMVVVAE NHTVVGGLGE AVATTLLSAH VSCSFRQIAL PDAFLDAGAL PTLHDRYGIS TSVMAANIKK WLA
NT Sequence
ATGAACTCGG TCATCGATAA AAAGCCTCGC CTGAAGACCT CTGCGATGAT CGCGTCTATT GCAGGTGAAG GACAGATCAC GCGCTCCGCA CCGTTCGGTC ACGCACTCGC CGAGCTGGCG CGTACGAAAA CGAATGTGAT CGGAATGACG GCCGATCTCG GCAAGTACAC CGACCTGCAC ATCTTCGCGA AGGCGTTTCC GGAGCGCTAC TACCAGATGG GCATGGCGGA GCAGTTGCTG ATGGGCGCTG CGGCCGGGTT CGCTCACGAA GGCGCACAGC CGTTCGTCAC CACGTATGCG GTATTCGCGA CGCGACGCGC GTACGACTTC ATTCACCAGA CCATCGCCGA AGACGACCTG GATGTGAAAA TCGTCTGTGC TCTGCCGGGT TTGACCACGG GTTATGGTCC AAGTCACCAG GCCGCGGAAG ATCTTGCGCT GATGCGCGCG ATGCCGAACA TGACCGTCAT CGATCCGTGC GATGCGCTGG ATATCGAGCA GATGGTGCCC GCCATCGCAG CTCACAAGGG GCCTGTCTAT GCGCGCCTGT TGCGCGGCAA TGTACCTGCT GTGCTGGACG AGTACGACTA TCGGTTCGAA CTCGGCAAGG CAAAGATGCT TCGCGACGGC GCCGACGTGC TCATCGTTTC GTCGGGGATG ATGACGATGC GCGCGCTCGA AGCCGCCAAG GCACTAGAAG CGGACAACGT GGATGTCGCC GTGCTGCACG TGCCGACCAT CAAGCCGCTC GACACGGAAA GCATCATTCG CGAAGCGAGG CGCTCGGGGC GTATGGTCGT TGTTGCCGAG AATCATACCG TCGTCGGAGG CCTCGGCGAA GCAGTGGCGA CGACGTTGTT GAGCGCCCAT GTGTCCTGTT CATTCAGGCA GATCGCGCTG CCCGACGCAT TCCTCGATGC AGGGGCGCTG CCGACGCTGC ACGACCGCTA TGGCATTTCC ACGAGCGTGA TGGCGGCCAA CATCAAGAAG TGGCTGGCA
Details for BuphA.17230.b.A1.GE32181
HARVESTED ON: 4/26/2011
SEQUENCED ON: 5/2/2011
EXPECTED MW: 38kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 74
PERCENT COVERAGE: 81
Validated AA Sequence
MGTLEAQTQG PGSMNSVIDK KPRLKTSAMI ASIAGEGQIT RSAPFGHALA ELARTKTNVI GMTADLGKYT DLHIFAKAFP ERYYQMGMAE QLLMGAAAGF AHEGAQPFVT TYAVFATRRA YDFIHQTIAE DDLDVKIVCA LPGLTTGYGP SHQAAEDLAL MRAMPNMTVI DPCDALDIEQ MVPAIAAHKG PVYARLLRGN VPAVLDEYXY RXEXXKXKXX RDGXXXXRFX XXDXXXXXXX XXXXXXXLXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX
Validated NT Sequence
cnnnnnnnnn nncnnnnnnn nnnnngcggn nnnnccatgt ntnccntngn nnnnncnncn tcaccatcat atgggtaccc tggaagctca gacccagggt cctggttcga tgaactcggt catcgataaa aagcctcgcc tgaagacctc tgcgatgatc gcgtctattg caggtgaagg acagatcacg cgctccgcac cgttcggtca cgcactcgcc gagctggcgc gtacgaaaac gaatgtgatc ggaatgacgg ccgatctcgg caagtacacc gacctgcaca tcttcgcgaa ggcgtttccg gagcgctact accagatggg catggcggag cagttgctga tgggcgctgc ggccgggttc gctcacgaag gcgcacagcc gttcgtcacc acgtatgcgg tattcgcgac gcgacgcgcg tacgacttca ttcaccagac catcgccgaa gacgacctgg atgtgaaaat cgtctgtgct ctgccgggtt tgaccacggg ttatggtcca agtcaccagg ccgcggaaga tcttgcgctg atgcgcgcga tgccgaacat gaccgtcatc gatccgtgcg atgcgctgga tatcgagcag atggtgcccg ccatcgcagc tcacaagggg cctgtctatg cgcgcctgtt gcgcggcaat gtacctgctg tgctggacga gtacnactat cggntcgaan tcngcaaggn aaagannntt cgcgacggnn nanntgntcn tcgnttcgnn nggnntgatg annannnnnn gnactannan nnnnnacnng nangncncnn nctgnnnnnn cnncntnngn nnnnnnnnnn nannnnnnnt nnnnnnnnnn nnnnnnnngn nnnnnnnnnc nnnaannnnc ntnnnnncnn nnnnnnnnnt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn g
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMNSVIDKKP RLKTSAMIAS IAGEGQITRS APFGHALAEL ARTKTNVIGM TADLGKYTDL HIFAKAFPER YYQMGMAEQL LMGAAAGFAH EGAQPFVTTY AVFATRRAYD FIHQTIAEDD LDVKIVCALP GLTTGYGPSH QAAEDLALMR AMPNMTVIDP CDALDIEQMV PAIAAHKGPV YARLLRGNVP AVLDEYDYRF ELGKAKMLRD GADVLIVSSG MMTMRALEAA KALEADNVDV AVLHVPTIKP LDTESIIREA RRSGRMVVVA ENHTVVGGLG EAVATTLLSA HVSCSFRQIA LPDAFLDAGA LPTLHDRYGI STSVMAANIK KWLA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaactcggtc atcgataaaa agcctcgcct gaagacctct gcgatgatcg cgtctattgc aggtgaagga cagatcacgc gctccgcacc gttcggtcac gcactcgccg agctggcgcg tacgaaaacg aatgtgatcg gaatgacggc cgatctcggc aagtacaccg acctgcacat cttcgcgaag gcgtttccgg agcgctacta ccagatgggc atggcggagc agttgctgat gggcgctgcg gccgggttcg ctcacgaagg cgcacagccg ttcgtcacca cgtatgcggt attcgcgacg cgacgcgcgt acgacttcat tcaccagacc atcgccgaag acgacctgga tgtgaaaatc gtctgtgctc tgccgggttt gaccacgggt tatggtccaa gtcaccaggc cgcggaagat cttgcgctga tgcgcgcgat gccgaacatg accgtcatcg atccgtgcga tgcgctggat atcgagcaga tggtgcccgc catcgcagct cacaaggggc ctgtctatgc gcgcctgttg cgcggcaatg tacctgctgt gctggacgag tacgactatc ggttcgaact cggcaaggca aagatgcttc gcgacggcgc cgacgtgctc atcgtttcgt cggggatgat gacgatgcgc gcgctcgaag ccgccaaggc actagaagcg gacaacgtgg atgtcgccgt gctgcacgtg ccgaccatca agccgctcga cacggaaagc atcattcgcg aagcgaggcg ctcggggcgt atggtcgttg ttgccgagaa tcataccgtc gtcggaggcc tcggcgaagc agtggcgacg acgttgttga gcgcccatgt gtcctgttca ttcaggcaga tcgcgctgcc cgacgcattc ctcgatgcag gggcgctgcc gacgctgcac gaccgctatg gcatttccac gagcgtgatg gcggccaaca tcaagaagtg gctggcataa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa