SSGCID: Target Page

BuphA.17229.a

Hydroxymethylbutenyl pyrophosphate reductase (IspH/LytB) (IspG/H)

CENTER ID:	BuphA.17229.a
ORGANISM:	Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuphA.17229.a.A1.GE32178	Full length( BuphA.17229.a )		1	319

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuphA.17229.a.A1.PS01126	Full length( BuphA.17229.a )		1	319

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|391038.7.peg.617
RefSeq:	YP_001856824.1
UniProt:	B2JE02

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSITDTTLAE AEILLAQPRG FCAGVDRAIE IVERAIKLYG SPIYVRHEIV HNAYVVEDLR KKGAIFIEQL EEVPSGSTVI FSAHGVSKAV RAEADERGLR VYDATCPLVT KVHIEVAKMR ADGFDIVMIG HKGHPEVEGT MGQTAEGMYL VEDIDDVQAL QLADPDRIAY VTQTTLSVDD AAQIIGALKV KYPNIKEPKK QDICYATQNR QDAVKFMAPQ CDVVIVVGSP NSSNSNRLRE LAEKLGVPSY MVDSPDQIDP AWVADKRRIG VTAGASAPEA LAQAVIGRLR ELGVRNVRAL EGIEENIAFP LPRGLGLPA

NT Sequence

ATGAGCATCA CGGACACGAC TCTCGCCGAA GCTGAAATCC TGCTTGCGCA GCCGCGCGGA TTTTGCGCCG GCGTCGATCG GGCGATCGAG ATCGTCGAAC GGGCCATCAA GCTGTACGGT TCGCCGATCT ACGTACGTCA CGAAATCGTT CATAACGCGT ATGTCGTCGA AGACCTGCGC AAGAAGGGCG CGATCTTTAT CGAGCAACTG GAAGAAGTGC CGTCGGGCAG CACGGTGATT TTCAGTGCGC ATGGCGTGTC GAAGGCCGTG CGCGCCGAAG CTGACGAGCG CGGCCTGCGC GTGTACGACG CGACGTGCCC GCTCGTCACC AAGGTGCATA TCGAAGTCGC GAAGATGCGC GCGGACGGCT TTGACATCGT GATGATCGGC CACAAGGGTC ACCCTGAAGT GGAAGGCACG ATGGGGCAGA CGGCCGAGGG CATGTATCTC GTCGAAGATA TCGACGACGT GCAGGCGTTG CAGCTTGCAG ATCCGGACCG CATCGCATAC GTGACGCAAA CCACGCTATC CGTCGACGAC GCCGCGCAGA TCATCGGGGC GCTGAAGGTA AAATATCCGA ACATCAAGGA GCCGAAGAAA CAGGACATCT GCTACGCCAC GCAAAACCGC CAGGACGCCG TCAAGTTCAT GGCGCCGCAG TGCGACGTGG TGATTGTCGT CGGCAGCCCG AACAGTTCCA ATTCCAACCG CCTGCGCGAA CTGGCCGAAA AGCTGGGTGT GCCTTCGTAT ATGGTGGACT CGCCAGACCA GATCGACCCC GCATGGGTCG CCGACAAGCG ACGCATCGGC GTGACGGCGG GTGCATCGGC GCCCGAGGCG CTGGCTCAGG CCGTTATCGG CCGTCTGCGC GAACTCGGCG TGCGCAACGT GCGCGCGCTC GAAGGGATCG AGGAAAACAT CGCGTTTCCC TTGCCCCGCG GGTTGGGTTT GCCGGCC

Details for BuphA.17229.a.A1.GE32178

HARVESTED ON:	4/26/2011
SEQUENCED ON:	5/2/2011
EXPECTED MW:	36kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	96

Validated AA Sequence

MGTLEAQTQG PGSMSITDTT LAEAEILLAQ PRGFCAGVDR AIEIVERAIK LYGSPIYVRH EIVHNAYVVE DLRKKGAIFI EQLEEVPSGS TVIFSAHGVS KAVRAEADER GLRVYDATCP LVTKVHIEVA KMRADGFDIV MIGHKGHPEV EGTMGQTAEG MYLVEDIDDV QALQLADPDR IAYVTQTTLS VDDAAQIIGA LKVKYPNIKE PKKQDICYAT QNRQDAVKFM APQCDVVIVV GSPNSSNSNR LRELAEKLGV PSYMVDSPDQ IDPAWVADKR RIGVTAGASA PEALAQAVIG RLRELGVRNV RALEGIEENI AFPLPRGLG

Validated NT Sequence

aacccaaccc gcggggcaag ggaaacgcga tgttttcctc gatcccttcg agcgcgcgca cgttgcgcac gccgagttcg cgcagacggc cgataacggc ctgagccagc gcctcgggcg ccgatgcacc cgccgtcacg ccgatgcgtc gcttgtcggc gacccatgcg gggtcgatct ggtctggcga gtccaccata tacgaaggca cacccagctt ttcggccagt tcgcgcaggc ggttggaatt ggaactgttc gggctgccga cgacaatcac cacgtcgcac tgcggcgcca tgaacttgac ggcgtcctgg cggttttgcg tggcgtagca gatgtcctgt ttcttcggct ccttgatgtt cggatatttt accttcagcg ccccgatgat ctgcgcggcg tcgtcgacgg atagcgtggt ttgcgtcacg tatgcgatgc ggtccggatc tgcaagctgc aacgcctgca cgtcgtcgat atcttcgacg agatacatgc cctcggccgt ctgccccatc gtgccttcca cttcagggtg acccttgtgg ccgatcatca cgatgtcaaa gccgtccgcg cgcatcttcg cgacttcgat atgcaccttg gtgacgagcg ggcacgtcgc gtcgtacacg cgcaggccgc gctcgtcagc ttcggcgcgc acggccttcg acacgccatg cgcactgaaa atcaccgtgc tgcccgacgg cacttcttcc agttgctcga taaagatcgc gcccttcttg cgcaggtctt cgacgacata cgcgttatga acgatttcgt gacgtacgta gatcggcgaa ccgtacagct tgatggcccg ttcgacgatc tcgatcgccc gatcgacgcc ggcgcaaaat ccgcgcggct gcgcaagcag gatttcagct tcggcgagag tcgtgtccgt gatgctcatc gaaccaggac cctgggtctg agcttccagg gtacccatat gatggtga

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMSITDTTLA EAEILLAQPR GFCAGVDRAI EIVERAIKLY GSPIYVRHEI VHNAYVVEDL RKKGAIFIEQ LEEVPSGSTV IFSAHGVSKA VRAEADERGL RVYDATCPLV TKVHIEVAKM RADGFDIVMI GHKGHPEVEG TMGQTAEGMY LVEDIDDVQA LQLADPDRIA YVTQTTLSVD DAAQIIGALK VKYPNIKEPK KQDICYATQN RQDAVKFMAP QCDVVIVVGS PNSSNSNRLR ELAEKLGVPS YMVDSPDQID PAWVADKRRI GVTAGASAPE ALAQAVIGRL RELGVRNVRA LEGIEENIAF PLPRGLGLPA

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcatcacg gacacgactc tcgccgaagc tgaaatcctg cttgcgcagc cgcgcggatt ttgcgccggc gtcgatcggg cgatcgagat cgtcgaacgg gccatcaagc tgtacggttc gccgatctac gtacgtcacg aaatcgttca taacgcgtat gtcgtcgaag acctgcgcaa gaagggcgcg atctttatcg agcaactgga agaagtgccg tcgggcagca cggtgatttt cagtgcgcat ggcgtgtcga aggccgtgcg cgccgaagct gacgagcgcg gcctgcgcgt gtacgacgcg acgtgcccgc tcgtcaccaa ggtgcatatc gaagtcgcga agatgcgcgc ggacggcttt gacatcgtga tgatcggcca caagggtcac cctgaagtgg aaggcacgat ggggcagacg gccgagggca tgtatctcgt cgaagatatc gacgacgtgc aggcgttgca gcttgcagat ccggaccgca tcgcatacgt gacgcaaacc acgctatccg tcgacgacgc cgcgcagatc atcggggcgc tgaaggtaaa atatccgaac atcaaggagc cgaagaaaca ggacatctgc tacgccacgc aaaaccgcca ggacgccgtc aagttcatgg cgccgcagtg cgacgtggtg attgtcgtcg gcagcccgaa cagttccaat tccaaccgcc tgcgcgaact ggccgaaaag ctgggtgtgc cttcgtatat ggtggactcg ccagaccaga tcgaccccgc atgggtcgcc gacaagcgac gcatcggcgt gacggcgggt gcatcggcgc ccgaggcgct ggctcaggcc gttatcggcc gtctgcgcga actcggcgtg cgcaacgtgc gcgcgctcga agggatcgag gaaaacatcg cgtttccctt gccccgcggg ttgggtttgc cggcctaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa

Details for BuphA.17229.a.A1.PS01126

PURIFICATION DATe:	6/23/2011
CONCENTRATION:	39.57mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	3
VIAL VOLUME:	100µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	96

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGCATCACG GACACGACTC TCGCCGAAGC TGAAATCCTG CTTGCGCAGC CGCGCGGATT TTGCGCCGGC GTCGATCGGG CGATCGAGAT CGTCGAACGG GCCATCAAGC TGTACGGTTC GCCGATCTAC GTACGTCACG AAATCGTTCA TAACGCGTAT GTCGTCGAAG ACCTGCGCAA GAAGGGCGCG ATCTTTATCG AGCAACTGGA AGAAGTGCCG TCGGGCAGCA CGGTGATTTT CAGTGCGCAT GGCGTGTCGA AGGCCGTGCG CGCCGAAGCT GACGAGCGCG GCCTGCGCGT GTACGACGCG ACGTGCCCGC TCGTCACCAA GGTGCATATC GAAGTCGCGA AGATGCGCGC GGACGGCTTT GACATCGTGA TGATCGGCCA CAAGGGTCAC CCTGAAGTGG AAGGCACGAT GGGGCAGACG GCCGAGGGCA TGTATCTCGT CGAAGATATC GACGACGTGC AGGCGTTGCA GCTTGCAGAT CCGGACCGCA TCGCATACGT GACGCAAACC ACGCTATCCG TCGACGACGC CGCGCAGATC ATCGGGGCGC TGAAGGTAAA ATATCCGAAC ATCAAGGAGC CGAAGAAACA GGACATCTGC TACGCCACGC AAAACCGCCA GGACGCCGTC AAGTTCATGG CGCCGCAGTG CGACGTGGTG ATTGTCGTCG GCAGCCCGAA CAGTTCCAAT TCCAACCGCC TGCGCGAACT GGCCGAAAAG CTGGGTGTGC CTTCGTATAT GGTGGACTCG CCAGACCAGA TCGACCCCGC ATGGGTCGCC GACAAGCGAC GCATCGGCGT GACGGCGGGT GCATCGGCGC CCGAGGCGCT GGCTCAGGCC GTTATCGGCC GTCTGCGCGA ACTCGGCGTG CGCAACGTGC GCGCGCTCGA AGGGATCGAG GAAAACATCG CGTTTCCCTT GCCCCGCGGG TTGGGTTTGC CGGCCTAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA