SSGCID: Target Page

BuphA.16000.a

Squalene/phytoene synthase

CENTER ID:	BuphA.16000.a
ORGANISM:	Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuphA.16000.a.B1.GE40989	Full length( BuphA.16000.a )		1	302

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|391038.7.peg.1061
RefSeq:	YP_001857241.1
UniProt:	B2JGL0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLQFSVFRAH LTHSRARLLS PQANPVNFDE YCQQKAAPPG SSTYYALRQA PVARQPLLTA LFALRREFEE TVKETTDPTI GRTKLAWWQK EAAALAAGEP THPVSQALAA HLPDVQAEYP ALQGIIAGFE MDLDQARYLD YPNLRRYMAG VGGTFASVVA RATAHEPSQA ASWAAPLGEA LMLAQIVVEL GNDARHGRIY IPIDEMQRFN VTAADLINRK YTDAFTEMMR FQTARARDAI RKALDALPVG ERHSQSTLRA LAALALALLD EIERDGYHVL HQRIALTPIR KLWVAWRAAR KH

NT Sequence

ATGCTACAAT TTTCGGTCTT TCGTGCACAC CTCACGCACA GCCGTGCGCG GCTCCTTTCC CCACAAGCCA ATCCCGTGAA TTTCGACGAA TATTGCCAGC AGAAAGCAGC GCCCCCCGGT TCGAGCACTT ACTACGCGTT GCGTCAGGCG CCCGTCGCGC GGCAGCCGCT GCTCACCGCG CTGTTCGCAC TGCGTCGCGA GTTCGAAGAG ACGGTGAAGG AAACGACGGA CCCGACCATC GGCCGCACGA AGCTGGCGTG GTGGCAAAAG GAAGCAGCGG CGCTCGCGGC CGGCGAGCCG ACGCACCCCG TCTCGCAGGC ACTCGCCGCG CATTTGCCCG ACGTGCAGGC GGAGTATCCC GCGCTGCAGG GCATAATCGC GGGGTTCGAG ATGGATCTCG ACCAGGCGCG CTATCTTGAC TATCCGAACT TACGGCGCTA CATGGCGGGC GTCGGCGGCA CCTTCGCGTC CGTCGTCGCC CGCGCAACGG CGCACGAGCC GTCGCAGGCG GCGTCGTGGG CTGCGCCGCT CGGCGAAGCG TTGATGCTCG CGCAGATCGT CGTGGAGCTC GGCAACGACG CGCGGCACGG ACGCATCTAC ATCCCCATCG ACGAGATGCA GCGCTTCAAC GTGACGGCCG CGGATCTGAT CAACCGAAAA TACACGGACG CGTTCACCGA GATGATGCGC TTTCAGACGG CGCGCGCACG CGACGCGATT CGCAAGGCGC TCGACGCACT CCCCGTCGGC GAGCGCCACT CGCAAAGCAC GCTGCGCGCG CTGGCAGCGC TGGCGCTGGC GTTGCTCGAT GAAATCGAAC GCGACGGCTA CCATGTGCTG CATCAGCGCA TCGCGCTCAC GCCGATCCGC AAGTTATGGG TCGCGTGGCG CGCGGCACGC AAACAC

Details for BuphA.16000.a.B1.GE40989

HARVESTED ON:	1/4/2017
SEQUENCED ON:	1/5/2017
EXPECTED MW:	35kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHML QFSVFRAHLT HSRARLLSPQ ANPVNFDEYC QQKAAPPGSS TYYALRQAPV ARQPLLTALF ALRREFEETV KETTDPTIGR TKLAWWQKEA AALAAGEPTH PVSQALAAHL PDVQAEYPAL QGIIAGFEMD LDQARYLDYP NLRRYMAGVG GTFASVVARA TAHEPSQAAS WAAPLGEALM LAQIVVELGN DARHGRIYIP IDEMQRFNVT AADLINRKYT DAFTEMMRFQ TARARDAIRK ALDALPVGER HSQSTLRALA ALALALLDEI ERDGYHVLHQ RIALTPIRKL WVAWRAARKH

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgctac aattttcggt ctttcgtgca cacctcacgc acagccgtgc gcggctcctt tccccacaag ccaatcccgt gaatttcgac gaatattgcc agcagaaagc agcgcccccc ggttcgagca cttactacgc gttgcgtcag gcgcccgtcg cgcggcagcc gctgctcacc gcgctgttcg cactgcgtcg cgagttcgaa gagacggtga aggaaacgac ggacccgacc atcggccgca cgaagctggc gtggtggcaa aaggaagcag cggcgctcgc ggccggcgag ccgacgcacc ccgtctcgca ggcactcgcc gcgcatttgc ccgacgtgca ggcggagtat cccgcgctgc agggcataat cgcggggttc gagatggatc tcgaccaggc gcgctatctt gactatccga acttacggcg ctacatggcg ggcgtcggcg gcaccttcgc gtccgtcgtc gcccgcgcaa cggcgcacga gccgtcgcag gcggcgtcgt gggctgcgcc gctcggcgaa gcgttgatgc tcgcgcagat cgtcgtggag ctcggcaacg acgcgcggca cggacgcatc tacatcccca tcgacgagat gcagcgcttc aacgtgacgg ccgcggatct gatcaaccga aaatacacgg acgcgttcac cgagatgatg cgctttcaga cggcgcgcgc acgcgacgcg attcgcaagg cgctcgacgc actccccgtc ggcgagcgcc actcgcaaag cacgctgcgc gcgctggcag cgctggcgct ggcgttgctc gatgaaatcg aacgcgacgg ctaccatgtg ctgcatcagc gcatcgcgct cacgccgatc cgcaagttat gggtcgcgtg gcgcgcggca cgcaaacact gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat