BuphA.15997.b

Beta-lactamase domain protein

CENTER ID: BuphA.15997.b
ORGANISM: Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS: diffraction
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuphA.15997.b.B1.GE40117 Full length( BuphA.15997.b ) 1 357
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
RefSeq: YP_001860974.1
RefSeq: WP_012404097.1
UniProt: B2JS09

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNALEHQLEY PFADRLPDGA DPVEVGPGVY WLRMPLPFAL DHINLWLLED EIDRVLGWTV VDCGAATAET TQRWERLFDS VLDGLPILRV LVTHCHPDHI GLAHWLCSGG DKRRWRAPLW ISFGEYTLGR LLSSGAVVNV GGETVSNHFR VNGLLDDVAL EGVKNRENYY RRLVPDVPGQ YRRIMEGDEL MIGERKWRVV TGFGHSPEHC ALYCKEDGLL ISGDMVLPRI STNISVFDLE PDGNPLELYL SSLAAYAAMS EDTLVLPSHG RPFTGLRTRI QQLRDHHAER LAEVMALCSQ QPSSAADIVP VMFKRKLDMH QMTFAMGEAL AHLHLLWNRG SLTRIVDAAG ILKFKSR
NT Sequence
ATGAATGCGC TTGAGCACCA ACTCGAATAT CCATTTGCTG ATCGCTTGCC AGACGGGGCG GATCCAGTAG AGGTCGGTCC TGGGGTCTAC TGGTTGAGGA TGCCGTTGCC TTTTGCATTG GACCATATCA ACCTCTGGCT GCTAGAAGAC GAAATTGACC GGGTATTGGG CTGGACGGTT GTCGACTGCG GTGCTGCAAC GGCCGAAACG ACGCAGAGAT GGGAGCGTCT GTTCGATAGT GTTCTTGATG GTCTGCCCAT CCTGCGTGTA CTTGTGACTC ACTGCCATCC TGACCATATC GGGCTCGCGC ATTGGTTATG CTCTGGCGGC GACAAGCGGC GCTGGAGGGC GCCTCTTTGG ATTTCGTTCG GTGAATATAC CCTTGGGCGG CTGCTGAGCA GCGGAGCGGT CGTCAATGTT GGCGGGGAGA CTGTTTCCAA CCATTTCAGG GTGAATGGCC TGCTAGATGA TGTTGCATTG GAGGGGGTGA AGAATCGAGA GAACTACTAT CGCCGGCTTG TCCCGGATGT GCCTGGGCAG TATCGCCGCA TTATGGAAGG CGATGAATTG ATGATTGGCG AGCGTAAGTG GCGAGTCGTT ACCGGATTTG GACATTCCCC GGAGCACTGT GCCCTTTATT GTAAGGAAGA TGGGCTGCTG ATTTCAGGTG ATATGGTTCT TCCGCGAATT TCAACAAATA TTTCCGTTTT TGATCTGGAG CCGGACGGTA ACCCGCTGGA ACTCTACCTT TCGTCTCTTG CTGCATACGC CGCCATGTCC GAGGACACCC TTGTTCTACC ATCACACGGT AGGCCTTTCA CTGGTCTGAG GACGCGAATA CAGCAGCTTC GCGATCATCA CGCTGAGCGG CTAGCGGAAG TGATGGCCTT GTGTTCGCAG CAACCGAGCT CAGCTGCAGA TATCGTCCCG GTGATGTTCA AGCGGAAACT AGACATGCAT CAGATGACAT TCGCCATGGG TGAGGCCTTG GCGCATCTGC ACCTGCTCTG GAATCGGGGC AGTCTGACTC GCATTGTCGA TGCTGCAGGG ATCCTGAAGT TCAAGTCGAG G
Details for BuphA.15997.b.B1.GE40117
HARVESTED ON: 4/6/2016
SEQUENCED ON: 4/13/2016
EXPECTED MW: 41kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMN ALEHQLEYPF ADRLPDGADP VEVGPGVYWL RMPLPFALDH INLWLLEDEI DRVLGWTVVD CGAATAETTQ RWERLFDSVL DGLPILRVLV THCHPDHIGL AHWLCSGGDK RRWRAPLWIS FGEYTLGRLL SSGAVVNVGG ETVSNHFRVN GLLDDVALEG VKNRENYYRR LVPDVPGQYR RIMEGDELMI GERKWRVVTG FGHSPEHCAL YCKEDGLLIS GDMVLPRIST NISVFDLEPD GNPLELYLSS LAAYAAMSED TLVLPSHGRP FTGLRTRIQQ LRDHHAERLA EVMALCSQQP SSAADIVPVM FKRKLDMHQM TFAMGEALAH LHLLWNRGSL TRIVDAAGIL KFKSR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaatg cgcttgagca ccaactcgaa tatccatttg ctgatcgctt gccagacggg gcggatccag tagaggtcgg tcctggggtc tactggttga ggatgccgtt gccttttgca ttggaccata tcaacctctg gctgctagaa gacgaaattg accgggtatt gggctggacg gttgtcgact gcggtgctgc aacggccgaa acgacgcaga gatgggagcg tctgttcgat agtgttcttg atggtctgcc catcctgcgt gtacttgtga ctcactgcca tcctgaccat atcgggctcg cgcattggtt atgctctggc ggcgacaagc ggcgctggag ggcgcctctt tggatttcgt tcggtgaata tacccttggg cggctgctga gcagcggagc ggtcgtcaat gttggcgggg agactgtttc caaccatttc agggtgaatg gcctgctaga tgatgttgca ttggaggggg tgaagaatcg agagaactac tatcgccggc ttgtcccgga tgtgcctggg cagtatcgcc gcattatgga aggcgatgaa ttgatgattg gcgagcgtaa gtggcgagtc gttaccggat ttggacattc cccggagcac tgtgcccttt attgtaagga agatgggctg ctgatttcag gtgatatggt tcttccgcga atttcaacaa atatttccgt ttttgatctg gagccggacg gtaacccgct ggaactctac ctttcgtctc ttgctgcata cgccgccatg tccgaggaca cccttgttct accatcacac ggtaggcctt tcactggtct gaggacgcga atacagcagc ttcgcgatca tcacgctgag cggctagcgg aagtgatggc cttgtgttcg cagcaaccga gctcagctgc agatatcgtc ccggtgatgt tcaagcggaa actagacatg catcagatga cattcgccat gggtgaggcc ttggcgcatc tgcacctgct ctggaatcgg ggcagtctga ctcgcattgt cgatgctgca gggatcctga agttcaagtc gaggtgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat