BuphA.15997.a
Beta-lactamase domain protein
| CENTER ID: | BuphA.15997.a |
| ORGANISM: | Burkholderia phymatum STM815 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuphA.15997.a.B1.GE40116 | Full length( BuphA.15997.a ) | 1 | 356 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuphA.15997.a.B1.PS38000 | Full length( BuphA.15997.a ) | 1 | 356 |
Structures
6AO1
DEPOSITED: 8/15/2017
DETERMINATION: XRay
CLONE: BuphA.15997.a.B1.GE40116
PROTEIN: BuphA.15997.a.B1.PS38000
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_155546 |
| PATRIC ID: | fig|391038.7.peg.197 |
| RefSeq: | YP_001856421.1 |
| UniProt: | B2JKI6 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNALEHQLDY PFADGMPAAG TTQEVAPGVY WLRMPLPFAL DHINLWLLRD EIDGQKGWTI VDCGIASGEI KANWETVFDT ALEGLPVLRV IVTHCHPDHL GLANWLCEGG DKKRWNVRLW ITLGEYMLGR VMAAGDGSNA GGEGAARHFA RHGLRDEASL DKLRNRKSYY ADLVPAVPGQ YRRLRDGDAL SIGARTWRVV TGFGHSPEHC ALHAEADGVL ISGDMVLPRI STNVSVFDIE PEGNPLALYL ESLGRYETMA ADTLVLPSHG KPFRGLHTRI GQLRDHHAAR LAEVRAACAD KPCSAADIVP IMFRRALDIH QMTFAMGEAL AHLHLLWLQG ELTRVQGEDG VIRFRA
NT Sequence
ATGAATGCTC TCGAACATCA ACTCGACTAC CCATTTGCTG ACGGGATGCC CGCCGCCGGC ACGACCCAGG AAGTCGCACC GGGCGTCTAC TGGCTGCGGA TGCCGCTGCC GTTCGCGCTC GATCACATCA ACCTGTGGCT GCTGCGTGAC GAGATCGACG GGCAGAAGGG CTGGACGATC GTCGACTGCG GCATCGCTTC CGGCGAGATC AAGGCGAACT GGGAAACGGT GTTCGACACG GCGCTGGAAG GCTTGCCCGT GCTGCGCGTG ATCGTCACGC ATTGCCATCC GGACCACCTC GGTCTCGCGA ACTGGCTATG CGAAGGCGGC GACAAGAAGC GTTGGAACGT GCGTCTATGG ATCACTCTCG GCGAGTACAT GCTGGGCCGC GTGATGGCGG CGGGCGACGG TTCGAACGCG GGCGGCGAGG GCGCGGCGCG GCACTTCGCG CGGCACGGTC TGCGCGACGA GGCATCGCTC GACAAACTGC GCAATCGCAA GAGCTATTAC GCGGACCTGG TGCCGGCCGT GCCGGGCCAG TATCGGCGTC TGCGCGACGG CGATGCGCTG TCGATCGGGG CTCGCACGTG GCGTGTCGTG ACGGGGTTCG GCCATTCTCC GGAGCACTGC GCGCTGCATG CGGAAGCGGA CGGCGTGCTG ATTTCCGGCG ACATGGTGCT GCCTCGTATT TCGACGAACG TGTCGGTGTT CGACATCGAG CCGGAAGGTA ATCCGCTGGC GTTGTATCTC GAATCGCTCG GCCGCTACGA AACGATGGCC GCGGACACGC TCGTGCTGCC TTCGCACGGC AAGCCGTTTC GCGGACTGCA CACGCGCATC GGCCAACTGC GCGATCACCA CGCGGCGCGG CTCGCGGAAG TGCGCGCGGC GTGCGCCGAC AAGCCATGCA GCGCGGCGGA CATCGTCCCG ATCATGTTCA GGCGCGCGCT CGACATTCAC CAGATGACGT TCGCGATGGG CGAGGCGCTC GCACATCTGC ATTTGCTGTG GCTGCAAGGC GAGCTGACGC GCGTGCAGGG CGAGGATGGC GTGATTCGCT TCCGTGCG
Details for BuphA.15997.a.B1.GE40116
| HARVESTED ON: | 4/6/2016 |
| SEQUENCED ON: | 4/13/2016 |
| EXPECTED MW: | 40kDa |
| OBSERVED MW: | 44kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Validated AA Sequence
MAHHHHHHMN ALEHQLDYPF ADGMPAAGTT QEVAPGVYWL RMPLPFALDH INLWLLRDEI DGQKGWTIVD CGIASGEIKA NWETVFDTAL EGLPVLRVIV THCHPDHLGL ANWLCEGGDK KRWNVRLWIT LGEYMLGRVM AAGDGSNAGG EGAARHFARH GLRDEASLDK LRNRKSYYAD LVPAVPGQYR RLRDGDALSI GARTWRVVTG FGHSPEHCAL HAEADGVLIS GDMVLPRIST NVSVFDIEPE GNPLALYLES LGRYETMAAD TLVLPSHGKP FRGLHTRIGQ LRDHHAARLA EVRAACADKP CSAADIVPIM FRRALDIHQM TFAMGEALAH LHLLWLQGEL TRVQGE
Validated NT Sequence
cctcgccctg cacgcgcgtc agctcgcctt gcagccacag caaatgcaga tgtgcgagcg cctcgcccat cgcgaacgtc atctggtgaa tgtcgagcgc gcgcctgaac atgatcggga cgatgtccgc cgcgctgcat ggcttgtcgg cgcacgccgc gcgcacttcc gcgagccgcg ccgcgtggtg atcgcgcagt tggccgatgc gcgtgtgcag tccgcgaaac ggcttgccgt gcgaaggcag cacgagcgtg tccgcggcca tcgtttcgta gcggccgagc gattcgagat acaacgccag cggattacct tccggctcga tgtcgaacac cgacacgttc gtcgaaatac gaggcagcac catgtcgccg gaaatcagca cgccgtccgc ttccgcatgc agcgcgcagt gctccggaga atggccgaac cccgtcacga cacgccacgt gcgagccccg atcgacagcg catcgccgtc gcgcagacgc cgatactggc ccggcacggc cggcaccagg tccgcgtaat agctcttgcg attgcgcagt ttgtcgagcg atgcctcgtc gcgcagaccg tgccgcgcga agtgccgcgc cgcgccctcg ccgcccgcgt tcgaaccgtc gcccgccgcc atcacgcggc ccagcatgta ctcgccgaga gtgatccata gacgcacgtt ccaacgcttc ttgtcgccgc cttcgcatag ccagttcgcg agaccgaggt ggtccggatg gcaatgcgtg acgatcacgc gcagcacggg caagccttcc agcgccgtgt cgaacaccgt ttcccagttc gccttgatct cgccggaagc gatgccgcag tcgacgatcg tccagccctt ctgcccgtcg atctcgtcac gcagcagcca caggttgatg tgatcgagcg cgaacggcag cggcatccgc agccagtaga cgcccggtgc gacttcctgg gtcgtgccgg cggcgggcat cccgtcagca aatgggtagt cgagttgatg ttcgagagca ttcatatggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHMN ALEHQLDYPF ADGMPAAGTT QEVAPGVYWL RMPLPFALDH INLWLLRDEI DGQKGWTIVD CGIASGEIKA NWETVFDTAL EGLPVLRVIV THCHPDHLGL ANWLCEGGDK KRWNVRLWIT LGEYMLGRVM AAGDGSNAGG EGAARHFARH GLRDEASLDK LRNRKSYYAD LVPAVPGQYR RLRDGDALSI GARTWRVVTG FGHSPEHCAL HAEADGVLIS GDMVLPRIST NVSVFDIEPE GNPLALYLES LGRYETMAAD TLVLPSHGKP FRGLHTRIGQ LRDHHAARLA EVRAACADKP CSAADIVPIM FRRALDIHQM TFAMGEALAH LHLLWLQGEL TRVQGEDGVI RFRA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaatg ctctcgaaca tcaactcgac tacccatttg ctgacgggat gcccgccgcc ggcacgaccc aggaagtcgc accgggcgtc tactggctgc ggatgccgct gccgttcgcg ctcgatcaca tcaacctgtg gctgctgcgt gacgagatcg acgggcagaa gggctggacg atcgtcgact gcggcatcgc ttccggcgag atcaaggcga actgggaaac ggtgttcgac acggcgctgg aaggcttgcc cgtgctgcgc gtgatcgtca cgcattgcca tccggaccac ctcggtctcg cgaactggct atgcgaaggc ggcgacaaga agcgttggaa cgtgcgtcta tggatcactc tcggcgagta catgctgggc cgcgtgatgg cggcgggcga cggttcgaac gcgggcggcg agggcgcggc gcggcacttc gcgcggcacg gtctgcgcga cgaggcatcg ctcgacaaac tgcgcaatcg caagagctat tacgcggacc tggtgccggc cgtgccgggc cagtatcggc gtctgcgcga cggcgatgcg ctgtcgatcg gggctcgcac gtggcgtgtc gtgacggggt tcggccattc tccggagcac tgcgcgctgc atgcggaagc ggacggcgtg ctgatttccg gcgacatggt gctgcctcgt atttcgacga acgtgtcggt gttcgacatc gagccggaag gtaatccgct ggcgttgtat ctcgaatcgc tcggccgcta cgaaacgatg gccgcggaca cgctcgtgct gccttcgcac ggcaagccgt ttcgcggact gcacacgcgc atcggccaac tgcgcgatca ccacgcggcg cggctcgcgg aagtgcgcgc ggcgtgcgcc gacaagccat gcagcgcggc ggacatcgtc ccgatcatgt tcaggcgcgc gctcgacatt caccagatga cgttcgcgat gggcgaggcg ctcgcacatc tgcatttgct gtggctgcaa ggcgagctga cgcgcgtgca gggcgaggat ggcgtgattc gcttccgtgc gtgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat
Details for BuphA.15997.a.B1.PS38000
| PURIFICATION DATe: | 8/3/2016 |
| CONCENTRATION: | 37.2mg/ml |
| OBSERVED MW: | 40kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | SEC Buffer |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 5 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMN ALEHQLDYPF ADGMPAAGTT QEVAPGVYWL RMPLPFALDH INLWLLRDEI DGQKGWTIVD CGIASGEIKA NWETVFDTAL EGLPVLRVIV THCHPDHLGL ANWLCEGGDK KRWNVRLWIT LGEYMLGRVM AAGDGSNAGG EGAARHFARH GLRDEASLDK LRNRKSYYAD LVPAVPGQYR RLRDGDALSI GARTWRVVTG FGHSPEHCAL HAEADGVLIS GDMVLPRIST NVSVFDIEPE GNPLALYLES LGRYETMAAD TLVLPSHGKP FRGLHTRIGQ LRDHHAARLA EVRAACADKP CSAADIVPIM FRRALDIHQM TFAMGEALAH LHLLWLQGEL TRVQGE
Validated NT Sequence
cctcgccctg cacgcgcgtc agctcgcctt gcagccacag caaatgcaga tgtgcgagcg cctcgcccat cgcgaacgtc atctggtgaa tgtcgagcgc gcgcctgaac atgatcggga cgatgtccgc cgcgctgcat ggcttgtcgg cgcacgccgc gcgcacttcc gcgagccgcg ccgcgtggtg atcgcgcagt tggccgatgc gcgtgtgcag tccgcgaaac ggcttgccgt gcgaaggcag cacgagcgtg tccgcggcca tcgtttcgta gcggccgagc gattcgagat acaacgccag cggattacct tccggctcga tgtcgaacac cgacacgttc gtcgaaatac gaggcagcac catgtcgccg gaaatcagca cgccgtccgc ttccgcatgc agcgcgcagt gctccggaga atggccgaac cccgtcacga cacgccacgt gcgagccccg atcgacagcg catcgccgtc gcgcagacgc cgatactggc ccggcacggc cggcaccagg tccgcgtaat agctcttgcg attgcgcagt ttgtcgagcg atgcctcgtc gcgcagaccg tgccgcgcga agtgccgcgc cgcgccctcg ccgcccgcgt tcgaaccgtc gcccgccgcc atcacgcggc ccagcatgta ctcgccgaga gtgatccata gacgcacgtt ccaacgcttc ttgtcgccgc cttcgcatag ccagttcgcg agaccgaggt ggtccggatg gcaatgcgtg acgatcacgc gcagcacggg caagccttcc agcgccgtgt cgaacaccgt ttcccagttc gccttgatct cgccggaagc gatgccgcag tcgacgatcg tccagccctt ctgcccgtcg atctcgtcac gcagcagcca caggttgatg tgatcgagcg cgaacggcag cggcatccgc agccagtaga cgcccggtgc gacttcctgg gtcgtgccgg cggcgggcat cccgtcagca aatgggtagt cgagttgatg ttcgagagca ttcatatggt ggtggtggtg gtgagccat
Expressed Protein Sequence
MAHHHHHHMN ALEHQLDYPF ADGMPAAGTT QEVAPGVYWL RMPLPFALDH INLWLLRDEI DGQKGWTIVD CGIASGEIKA NWETVFDTAL EGLPVLRVIV THCHPDHLGL ANWLCEGGDK KRWNVRLWIT LGEYMLGRVM AAGDGSNAGG EGAARHFARH GLRDEASLDK LRNRKSYYAD LVPAVPGQYR RLRDGDALSI GARTWRVVTG FGHSPEHCAL HAEADGVLIS GDMVLPRIST NVSVFDIEPE GNPLALYLES LGRYETMAAD TLVLPSHGKP FRGLHTRIGQ LRDHHAARLA EVRAACADKP CSAADIVPIM FRRALDIHQM TFAMGEALAH LHLLWLQGEL TRVQGEDGVI RFRA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAATG CTCTCGAACA TCAACTCGAC TACCCATTTG CTGACGGGAT GCCCGCCGCC GGCACGACCC AGGAAGTCGC ACCGGGCGTC TACTGGCTGC GGATGCCGCT GCCGTTCGCG CTCGATCACA TCAACCTGTG GCTGCTGCGT GACGAGATCG ACGGGCAGAA GGGCTGGACG ATCGTCGACT GCGGCATCGC TTCCGGCGAG ATCAAGGCGA ACTGGGAAAC GGTGTTCGAC ACGGCGCTGG AAGGCTTGCC CGTGCTGCGC GTGATCGTCA CGCATTGCCA TCCGGACCAC CTCGGTCTCG CGAACTGGCT ATGCGAAGGC GGCGACAAGA AGCGTTGGAA CGTGCGTCTA TGGATCACTC TCGGCGAGTA CATGCTGGGC CGCGTGATGG CGGCGGGCGA CGGTTCGAAC GCGGGCGGCG AGGGCGCGGC GCGGCACTTC GCGCGGCACG GTCTGCGCGA CGAGGCATCG CTCGACAAAC TGCGCAATCG CAAGAGCTAT TACGCGGACC TGGTGCCGGC CGTGCCGGGC CAGTATCGGC GTCTGCGCGA CGGCGATGCG CTGTCGATCG GGGCTCGCAC GTGGCGTGTC GTGACGGGGT TCGGCCATTC TCCGGAGCAC TGCGCGCTGC ATGCGGAAGC GGACGGCGTG CTGATTTCCG GCGACATGGT GCTGCCTCGT ATTTCGACGA ACGTGTCGGT GTTCGACATC GAGCCGGAAG GTAATCCGCT GGCGTTGTAT CTCGAATCGC TCGGCCGCTA CGAAACGATG GCCGCGGACA CGCTCGTGCT GCCTTCGCAC GGCAAGCCGT TTCGCGGACT GCACACGCGC ATCGGCCAAC TGCGCGATCA CCACGCGGCG CGGCTCGCGG AAGTGCGCGC GGCGTGCGCC GACAAGCCAT GCAGCGCGGC GGACATCGTC CCGATCATGT TCAGGCGCGC GCTCGACATT CACCAGATGA CGTTCGCGAT GGGCGAGGCG CTCGCACATC TGCATTTGCT GTGGCTGCAA GGCGAGCTGA CGCGCGTGCA GGGCGAGGAT GGCGTGATTC GCTTCCGTGC GTGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT