SSGCID: Target Page

BuphA.12800.z

Transcriptional regulator, LysR family

CENTER ID:	BuphA.12800.z
ORGANISM:	Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuphA.12800.z.A1.GE32177	Full length( BuphA.12800.z )		1	318

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|391038.7.peg.6706
RefSeq:	YP_001862466.1
UniProt:	B2JWU3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSTDSASDFE FFILLAKLKS MSGAARALDL TPPAATKRLG IIERRLGARL VNRTTRSVSL TPEGETYLRY ATQIVGQVRE MEEAISGAGR DPQGLLRINA TLGFGRTTIA PLVSDFAKRF PNVEVQFEVT DRPVDLIEGA FDMAIRFGEL PDSRLSARRI MSNRRFLCAS PKYLERFGTP ERVDDLQQHR CIIHRQNDDA YGVWRYIVND HIEAIKVKGA LSSNDGDIVL RWALDGHGIL IRSEWDLAKY VQSGRLKLVL PNTVLPSADL FVYYPRQRNQ TARARAFIDF LVDRFQAPFT PVDTAIDVRE RKKRVSRK

NT Sequence

GTGAGTACTG ACAGCGCCTC CGACTTCGAG TTTTTCATTC TGCTTGCGAA GCTCAAGAGC ATGTCGGGTG CTGCGCGCGC GCTCGACCTC ACGCCGCCCG CCGCGACGAA GCGGCTGGGC ATCATCGAGC GGCGGCTCGG TGCGCGTCTC GTCAACCGCA CCACGCGAAG CGTCAGTCTC ACGCCCGAGG GGGAAACGTA TCTGCGCTAC GCGACCCAGA TAGTCGGGCA GGTTCGTGAA ATGGAAGAGG CGATTTCTGG CGCTGGACGC GACCCGCAGG GTTTGTTGCG CATCAACGCG ACGCTCGGGT TTGGCAGAAC CACCATTGCG CCGCTCGTCT CGGACTTCGC AAAGCGTTTT CCGAACGTGG AGGTTCAGTT CGAAGTGACG GACCGACCCG TCGATTTGAT CGAAGGCGCG TTCGACATGG CTATCCGCTT CGGCGAACTG CCCGACAGCC GCCTCAGCGC GCGGCGCATC ATGAGCAATC GCCGCTTTCT GTGCGCGTCG CCGAAGTATC TCGAAAGGTT CGGAACGCCC GAGCGCGTGG ACGATTTGCA GCAGCATCGC TGCATCATTC ACCGGCAGAA CGACGACGCT TACGGCGTGT GGCGCTACAT CGTGAACGAT CACATCGAGG CAATCAAGGT GAAGGGCGCG CTGTCCAGCA ACGACGGCGA CATTGTGCTT CGCTGGGCGC TCGACGGCCA CGGCATCCTG ATCCGCTCCG AATGGGACCT GGCGAAGTAC GTGCAGAGCG GACGGCTCAA GCTGGTGCTG CCTAATACGG TCCTGCCGTC AGCCGACCTG TTCGTCTACT ACCCGAGGCA GCGCAATCAG ACGGCGCGTG CGCGTGCGTT CATCGATTTC CTCGTCGATC GTTTTCAGGC GCCCTTCACG CCAGTCGATA CGGCTATCGA CGTGCGCGAG CGCAAGAAGC GTGTTTCGCG CAAG

Details for BuphA.12800.z.A1.GE32177

HARVESTED ON:	4/26/2011
SEQUENCED ON:	5/2/2011
EXPECTED MW:	38kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	93

Validated AA Sequence

MVSTDSASDF EFFILLAKLK SMSGAARALD LTPPAATKRL GIIERRLGAR LVNRTTRSVS LTPEGETYLR YATQIVGQVR EMEEAISGAG RDPQGLLRIN ATLGFGRTTI APLVSDFAKR FPNVEVQFEV TDRPVDLIEG AFDMAIRFGE LPDSRLSARR IMSNRRFLCA SPKYLERFGT PERVDDLQQH RCIIHRQNDD AYGVWRYIVN DHIEAIKVKG ALSSNDGDIV LRWALDGHGI LIRSEWDLAK YVQSGRLKLV LPNTVLPSAD LFVYYPRQRN QTARARAFID FLVDRFQAPF TPVDTAIDVR ERKKRVSRK

Validated NT Sequence

ttattacttg cgcgaaacac gcttcttgcg ctcgcgcacg tcgatagccg tatcgactgg cgtgaagggc gcctgaaaac gatcgacgag gaaatcgatg aacgcacgcg cacgcgccgt ctgattgcgc tgcctcgggt agtagacgaa caggtcggct gacggcagga ccgtattagg cagcaccagc ttgagccgtc cgctctgcac gtacttcgcc aggtcccatt cggagcggat caggatgccg tggccgtcga gcgcccagcg aagcacaatg tcgccgtcgt tgctggacag cgcgcccttc accttgattg cctcgatgtg atcgttcacg atgtagcgcc acacgccgta agcgtcgtcg ttctgccggt gaatgatgca gcgatgctgc tgcaaatcgt ccacgcgctc gggcgttccg aacctttcga gatacttcgg cgacgcgcac agaaagcggc gattgctcat gatgcgccgc gcgctgaggc ggctgtcggg cagttcgccg aagcggatag ccatgtcgaa cgcgccttcg atcaaatcga cgggtcggtc cgtcacttcg aactgaacct ccacgttcgg aaaacgcttt gcgaagtccg agacgagcgg cgcaatggtg gttctgccaa acccgagcgt cgcgttgatg cgcaacaaac cctgcgggtc gcgtccagcg ccagaaatcg cctcttccat ttcacgaacc tgcccgacta tctgggtcgc gtagcgcaga tacgtttccc cctcgggcgt gagactgacg cttcgcgtgg tgcggttgac gagacgcgca ccgagccgcc gctcgatgat gcccagccgc ttcgtcgcgg cgggcggcgt gaggtcgagc gcgcgcgcag cacccgacat gctcttgagc ttcgcaagca gaatgaaaaa ctcgaagtcg gaggcgctgt cagtactcac catcgaacca ggaccctggg tctgagcttc cagggtacc

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMVSTDSASD FEFFILLAKL KSMSGAARAL DLTPPAATKR LGIIERRLGA RLVNRTTRSV SLTPEGETYL RYATQIVGQV REMEEAISGA GRDPQGLLRI NATLGFGRTT IAPLVSDFAK RFPNVEVQFE VTDRPVDLIE GAFDMAIRFG ELPDSRLSAR RIMSNRRFLC ASPKYLERFG TPERVDDLQQ HRCIIHRQND DAYGVWRYIV NDHIEAIKVK GALSSNDGDI VLRWALDGHG ILIRSEWDLA KYVQSGRLKL VLPNTVLPSA DLFVYYPRQR NQTARARAFI DFLVDRFQAP FTPVDTAIDV RERKKRVSRK

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgagtact gacagcgcct ccgacttcga gtttttcatt ctgcttgcga agctcaagag catgtcgggt gctgcgcgcg cgctcgacct cacgccgccc gccgcgacga agcggctggg catcatcgag cggcggctcg gtgcgcgtct cgtcaaccgc accacgcgaa gcgtcagtct cacgcccgag ggggaaacgt atctgcgcta cgcgacccag atagtcgggc aggttcgtga aatggaagag gcgatttctg gcgctggacg cgacccgcag ggtttgttgc gcatcaacgc gacgctcggg tttggcagaa ccaccattgc gccgctcgtc tcggacttcg caaagcgttt tccgaacgtg gaggttcagt tcgaagtgac ggaccgaccc gtcgatttga tcgaaggcgc gttcgacatg gctatccgct tcggcgaact gcccgacagc cgcctcagcg cgcggcgcat catgagcaat cgccgctttc tgtgcgcgtc gccgaagtat ctcgaaaggt tcggaacgcc cgagcgcgtg gacgatttgc agcagcatcg ctgcatcatt caccggcaga acgacgacgc ttacggcgtg tggcgctaca tcgtgaacga tcacatcgag gcaatcaagg tgaagggcgc gctgtccagc aacgacggcg acattgtgct tcgctgggcg ctcgacggcc acggcatcct gatccgctcc gaatgggacc tggcgaagta cgtgcagagc ggacggctca agctggtgct gcctaatacg gtcctgccgt cagccgacct gttcgtctac tacccgaggc agcgcaatca gacggcgcgt gcgcgtgcgt tcatcgattt cctcgtcgat cgttttcagg cgcccttcac gccagtcgat acggctatcg acgtgcgcga gcgcaagaag cgtgtttcgc gcaagtaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa