SSGCID: Target Page

BuphA.12800.y

Transcriptional regulator, LysR family

CENTER ID:	BuphA.12800.y
ORGANISM:	Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuphA.12800.y.A1.GE32408	Full length( BuphA.12800.y )		1	324

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|391038.7.peg.6158
RefSeq:	YP_001861956.1
UniProt:	B2JVD3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDRIQAMEVF TRVVDANSFT RAAEQLGMPR ATVTTTIQNL EAVLGVRLMN RTTRRLSLTP EGAAYYEHCI RIITDIAETD ASFQAGNRKP SGALRVHMPN SLGRHLVIPA LQSFHQRYPD IALDLGLSDR PVDPVEEGID CMIRAGALED SSMVARRIGT LKRVTCASPD YLVRFGEPQS IDDLAMHHAV NFRVAHGTRP MPWIILVDGK PIEVRMNGVV TVNDSEAYVK CGMEGFGLIQ PMLFMVAPQL RDGSLKEVLP AFKPKPKPIS IVYPNNRHLS AKVRVFADWV AELFESTPAL AGSEGRRIAV PRREAQPDHG PIAA

NT Sequence

GTGGACCGCA TTCAGGCAAT GGAAGTGTTC ACTCGTGTGG TCGATGCAAA CAGCTTCACG CGTGCCGCCG AACAACTCGG CATGCCGCGC GCGACGGTGA CGACGACCAT CCAGAATCTC GAGGCGGTGC TCGGCGTGCG TCTGATGAAC CGGACCACGC GGCGTCTGTC GCTCACGCCG GAAGGCGCCG CGTACTACGA ACACTGCATC CGCATCATCA CGGACATCGC GGAAACGGAC GCGAGTTTCC AGGCAGGCAA CCGCAAGCCG AGCGGCGCGT TGCGCGTGCA CATGCCGAAC TCGCTCGGCC GTCACCTGGT GATCCCTGCA TTGCAGAGCT TTCATCAGCG CTATCCGGAC ATCGCGCTTG ATCTCGGCCT GTCGGACCGG CCCGTCGATC CCGTCGAGGA GGGCATCGAT TGCATGATCC GGGCAGGCGC GCTCGAAGAT TCGTCGATGG TCGCGCGGCG CATCGGCACG CTCAAGCGCG TCACCTGCGC ATCGCCCGAT TACCTCGTGC GCTTTGGCGA GCCGCAGAGC ATCGACGATC TCGCGATGCA TCACGCGGTG AATTTCCGCG TTGCGCACGG CACGCGCCCG ATGCCGTGGA TCATCCTCGT CGACGGCAAG CCGATCGAAG TGCGCATGAA CGGCGTGGTC ACGGTCAACG ATTCCGAGGC GTATGTGAAG TGCGGGATGG AAGGCTTCGG CCTGATTCAG CCAATGCTGT TCATGGTCGC GCCGCAACTG CGCGATGGTT CGCTGAAGGA AGTGCTGCCC GCGTTCAAGC CGAAGCCCAA GCCCATTTCG ATCGTGTACC CGAACAACCG GCATCTGTCC GCGAAGGTGC GCGTGTTCGC CGACTGGGTC GCCGAACTGT TCGAATCGAC GCCCGCGCTG GCAGGCAGCG AAGGGCGGCG GATCGCCGTG CCCAGGCGCG AGGCGCAACC GGATCATGGG CCGATTGCGG CA

Details for BuphA.12800.y.A1.GE32408

HARVESTED ON:	11/8/2011
SEQUENCED ON:	11/11/2011
EXPECTED MW:	38kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	21
PERCENT COVERAGE:	98

Validated AA Sequence

MIRLRLAPGH GDPPPFAACQ RGRRFEQFGD PVGEHAHLRG QMPVVRVHDR NGLGLRLERG QHFLQRTIAQ LRRDHEQHWL NQAEAFHPAL HIRLGIVDRD HAVHAHFDRL AVDEDDPRHR ARAVRNAEIH RVMHREIVDA LRLAKAHEVI GRCAGDALER ADAPRDHRRI FERACPDHAI DALLDGIDGP VRQAEIKRDV RIALMKALQC RDHQVTAERV RHVHAQRAAR LAVACLETRV RFRDVRDDAD AVFVVRGAFR RERQTPRGPV HQTHAEHRLE ILDGRRHRRA RHAELFGGTR EAVCIDHTSE HFHCLNAVHH RTRTLGLSFQ GTHMMVMVMS

Validated NT Sequence

tgccgcaatc ggcccatgat ccggttgcgc ctcgcgcctg ggcacggcga tccgccgccc ttcgctgcct gccagcgcgg gcgtcgattc gaacagttcg gcgacccagt cggcgaacac gcgcaccttc gcggacagat gccggttgtt cgggtacacg atcgaaatgg gcttgggctt cggcttgaac gcgggcagca cttccttcag cgaaccatcg cgcagttgcg gcgcgaccat gaacagcatt ggctgaatca ggccgaagcc ttccatcccg cacttcacat acgcctcgga atcgttgacc gtgaccacgc cgttcatgcg cacttcgatc ggcttgccgt cgacgaggat gatccacggc atcgggcgcg tgccgtgcgc aacgcggaaa ttcaccgcgt gatgcatcgc gagatcgtcg atgctctgcg gctcgccaaa gcgcacgagg taatcgggcg atgcgcaggt gacgcgcttg agcgtgccga tgcgccgcgc gaccatcgac gaatcttcga gcgcgcctgc ccggatcatg caatcgatgc cctcctcgac gggatcgacg ggccggtccg acaggccgag atcaagcgcg atgtccggat agcgctgatg aaagctctgc aatgcaggga tcaccaggtg acggccgagc gagttcggca tgtgcacgcg caacgcgccg ctcggcttgc ggttgcctgc ctggaaactc gcgtccgttt ccgcgatgtc cgtgatgatg cggatgcagt gttcgtagta cgcggcgcct tccggcgtga gcgacagacg ccgcgtggtc cggttcatca gacgcacgcc gagcaccgcc tcgagattct ggatggtcgt cgtcaccgtc gcgcgcggca tgccgagttg ttcggcggca cgcgtgaagc tgtttgcatc gaccacacga gtgaacactt ccattgcctg aatgcggtcc accatcgaac caggaccctg ggtctgagct tccagggtac ccatatgatg gtgatggtga tgagcc

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMVDRIQAME VFTRVVDANS FTRAAEQLGM PRATVTTTIQ NLEAVLGVRL MNRTTRRLSL TPEGAAYYEH CIRIITDIAE TDASFQAGNR KPSGALRVHM PNSLGRHLVI PALQSFHQRY PDIALDLGLS DRPVDPVEEG IDCMIRAGAL EDSSMVARRI GTLKRVTCAS PDYLVRFGEP QSIDDLAMHH AVNFRVAHGT RPMPWIILVD GKPIEVRMNG VVTVNDSEAY VKCGMEGFGL IQPMLFMVAP QLRDGSLKEV LPAFKPKPKP ISIVYPNNRH LSAKVRVFAD WVAELFESTP ALAGSEGRRI AVPRREAQPD HGPIAA

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtggaccgc attcaggcaa tggaagtgtt cactcgtgtg gtcgatgcaa acagcttcac gcgtgccgcc gaacaactcg gcatgccgcg cgcgacggtg acgacgacca tccagaatct cgaggcggtg ctcggcgtgc gtctgatgaa ccggaccacg cggcgtctgt cgctcacgcc ggaaggcgcc gcgtactacg aacactgcat ccgcatcatc acggacatcg cggaaacgga cgcgagtttc caggcaggca accgcaagcc gagcggcgcg ttgcgcgtgc acatgccgaa ctcgctcggc cgtcacctgg tgatccctgc attgcagagc tttcatcagc gctatccgga catcgcgctt gatctcggcc tgtcggaccg gcccgtcgat cccgtcgagg agggcatcga ttgcatgatc cgggcaggcg cgctcgaaga ttcgtcgatg gtcgcgcggc gcatcggcac gctcaagcgc gtcacctgcg catcgcccga ttacctcgtg cgctttggcg agccgcagag catcgacgat ctcgcgatgc atcacgcggt gaatttccgc gttgcgcacg gcacgcgccc gatgccgtgg atcatcctcg tcgacggcaa gccgatcgaa gtgcgcatga acggcgtggt cacggtcaac gattccgagg cgtatgtgaa gtgcgggatg gaaggcttcg gcctgattca gccaatgctg ttcatggtcg cgccgcaact gcgcgatggt tcgctgaagg aagtgctgcc cgcgttcaag ccgaagccca agcccatttc gatcgtgtac ccgaacaacc ggcatctgtc cgcgaaggtg cgcgtgttcg ccgactgggt cgccgaactg ttcgaatcga cgcccgcgct ggcaggcagc gaagggcggc ggatcgccgt gcccaggcgc gaggcgcaac cggatcatgg gccgattgcg gcataaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa