BuphA.12800.x

Transcriptional regulator, LysR family

CENTER ID: BuphA.12800.x
ORGANISM: Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuphA.12800.x.A1.GE32179 Full length( BuphA.12800.x ) 1 320
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|391038.7.peg.6006
RefSeq: YP_001861820.1
UniProt: B2JUZ6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDKILSMRIF SRVVESGSFS AVAEHMNCST GSVSRAVSSL EDQLQARLLQ RTTRKISLTE PGERYYQKCK KILADLEDAE AEAGDAHTCA RGTLRIHCVT DLGLAQLTHS IIEYRKRFPA VSVQLKFLPR MANLLEDEVD VSIVSAPTLP DSRNVCKLIG HCERVLVASP AFLQTHRIDM ASDLDEHALT PVSFRVEPGD HHVRLSLVKS ASREAAGQFA VNDTEATRIA TLAGAGVAAL PTHCVADDIR DGRLVQLFPE SRLQNTGVFA VYSSRHHIDA KIKTFVDFLT SHLGATLDTR LFNEHSNQTF NRLARVVENA
NT Sequence
ATGGACAAGA TCTTGAGCAT GCGCATCTTC TCGCGCGTCG TCGAGTCGGG CAGTTTCAGT GCCGTTGCCG AGCACATGAA CTGCAGCACG GGCAGCGTTT CGCGTGCCGT GTCGTCGCTC GAAGACCAAT TGCAGGCAAG GCTCCTGCAA CGGACGACGC GCAAGATATC GCTGACGGAG CCCGGCGAGC GCTACTACCA GAAGTGCAAG AAGATACTCG CGGACCTCGA AGACGCCGAG GCAGAAGCGG GCGACGCGCA TACCTGTGCG CGCGGCACGC TGCGCATTCA TTGCGTGACC GACCTCGGAC TCGCGCAGCT GACGCATTCG ATCATCGAGT ATCGCAAGCG CTTTCCCGCC GTCTCCGTGC AGCTGAAGTT CCTGCCGCGC ATGGCGAACC TGCTCGAAGA CGAAGTCGAC GTGTCGATCG TGTCCGCGCC GACGCTGCCC GACTCGCGCA ACGTCTGCAA GCTGATCGGC CATTGTGAGC GCGTGCTCGT CGCGTCGCCC GCTTTTCTTC AGACGCATCG GATCGACATG GCAAGCGACC TCGACGAACA CGCGCTGACG CCCGTGTCCT TTCGCGTCGA ACCGGGCGAT CATCACGTGA GGCTGAGCCT CGTGAAGTCG GCGAGCCGTG AGGCCGCGGG CCAGTTCGCC GTCAACGACA CAGAAGCGAC CCGGATCGCC ACGCTGGCCG GCGCGGGCGT GGCGGCATTG CCCACGCATT GCGTGGCCGA CGACATCCGC GACGGCAGGC TGGTGCAACT GTTCCCCGAA TCGCGTCTTC AAAATACGGG CGTGTTTGCC GTTTATTCAA GCAGGCATCA CATCGACGCG AAGATCAAGA CGTTCGTTGA CTTCCTGACT TCGCATCTGG GTGCAACGCT CGACACCCGT CTTTTCAACG AGCACTCCAA TCAAACTTTC AATCGTCTCG CGCGCGTAGT GGAGAATGCA
Details for BuphA.12800.x.A1.GE32179
HARVESTED ON: 4/26/2011
SEQUENCED ON: 5/2/2011
EXPECTED MW: 37kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 81
PERCENT COVERAGE: 100
Validated AA Sequence
MGTLEAQTQG PGSMDKILSM RIFSRVVESG SFSAVAEHMN CSTGSVSRAV SSLEDQLQAR LLQRTTRKIS LTEPGERYYQ KCKKILADLE DAEAEAGDAH TCARGTLRIH CVTDLGLAQL THSIIEYRKR FPAVSVQLKF LPRMANLLED EVDVSIVSAP TLPDSRNVCK LIGHCERVLV ASPAFLQTHR IDMASDLDEH ALTPVSFRVE PGDHHVRLSL VKSAXREAAG QFAVNDTEAT RIAXLXXAGV AALPTHCXXD DIXDXRLVXX FPXSRLQNTG VXXXXXAGIT XTXXSXXXXX XXXXXXXXXP XXXXXXXXTX SXXAXXXXXX XXXXXXXXXX XX
Validated NT Sequence
cnnnnnnnnn nnnnnnnnnn nnnnngcnnn nnnnnnntgt nnccntggnn nnncaccntc accatcatat gggtaccctg gaagctcaga cccagggtcc tggttcgatg gacaagatct tgagcatgcg catcttctcg cgcgtcgtcg agtcgggcag tttcagtgcc gttgccgagc acatgaactg cagcacgggc agcgtttcgc gtgccgtgtc gtcgctcgaa gaccaattgc aggcaaggct cctgcaacgg acgacgcgca agatatcgct gacggagccc ggcgagcgct actaccagaa gtgcaagaag atactcgcgg acctcgaaga cgccgaggca gaagcgggcg acgcgcatac ctgtgcgcgc ggcacgctgc gcattcattg cgtgaccgac ctcggactcg cgcagctgac gcattcgatc atcgagtatc gcaagcgctt tcccgccgtc tccgtgcagc tgaagttcct gccgcgcatg gcgaacctgc tcgaagacga agtcgacgtg tcgatcgtgt ccgcgccgac gctgcccgac tcgcgcaacg tctgcaagct gatcggccat tgtgagcgcg tgctcgtcgc gtcgcccgct tttcttcaga cgcatcggat cgacatggca agcgacctcg acgaacacgc gctgacgccc gtgtcctttc gcgtcgaacc gggcgatcat cacgtgaggc tgagcctcgt gaagtcngcg agncgtgagg ccgcgggcca gttcgccgtc aacgacacag aagcgacccg gatcgccncg ctgnncgnng cgggcgtggc ggcattgccc acgcattgcg nggncgacga catcngcgac ngcaggctgg tgcnacngtt ccccnaatcg cgtcttcaaa atacnggcgt gnttnnngnt nnnnnagcag gcatcacnnc gacncnnnna tcananntnn nnannnntga nntnnnnnnn ngngnnnnct nganaccnnc tttnnnnnnn nnnnnnnnnn nnacnnnntc gnnncnngcg tnnnncnnnn nnnnnncnnn nnnnnnnnnn nnnnnnnnnn nnntnnnnnn nanana
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDKILSMRI FSRVVESGSF SAVAEHMNCS TGSVSRAVSS LEDQLQARLL QRTTRKISLT EPGERYYQKC KKILADLEDA EAEAGDAHTC ARGTLRIHCV TDLGLAQLTH SIIEYRKRFP AVSVQLKFLP RMANLLEDEV DVSIVSAPTL PDSRNVCKLI GHCERVLVAS PAFLQTHRID MASDLDEHAL TPVSFRVEPG DHHVRLSLVK SASREAAGQF AVNDTEATRI ATLAGAGVAA LPTHCVADDI RDGRLVQLFP ESRLQNTGVF AVYSSRHHID AKIKTFVDFL TSHLGATLDT RLFNEHSNQT FNRLARVVEN A
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggacaagatc ttgagcatgc gcatcttctc gcgcgtcgtc gagtcgggca gtttcagtgc cgttgccgag cacatgaact gcagcacggg cagcgtttcg cgtgccgtgt cgtcgctcga agaccaattg caggcaaggc tcctgcaacg gacgacgcgc aagatatcgc tgacggagcc cggcgagcgc tactaccaga agtgcaagaa gatactcgcg gacctcgaag acgccgaggc agaagcgggc gacgcgcata cctgtgcgcg cggcacgctg cgcattcatt gcgtgaccga cctcggactc gcgcagctga cgcattcgat catcgagtat cgcaagcgct ttcccgccgt ctccgtgcag ctgaagttcc tgccgcgcat ggcgaacctg ctcgaagacg aagtcgacgt gtcgatcgtg tccgcgccga cgctgcccga ctcgcgcaac gtctgcaagc tgatcggcca ttgtgagcgc gtgctcgtcg cgtcgcccgc ttttcttcag acgcatcgga tcgacatggc aagcgacctc gacgaacacg cgctgacgcc cgtgtccttt cgcgtcgaac cgggcgatca tcacgtgagg ctgagcctcg tgaagtcggc gagccgtgag gccgcgggcc agttcgccgt caacgacaca gaagcgaccc ggatcgccac gctggccggc gcgggcgtgg cggcattgcc cacgcattgc gtggccgacg acatccgcga cggcaggctg gtgcaactgt tccccgaatc gcgtcttcaa aatacgggcg tgtttgccgt ttattcaagc aggcatcaca tcgacgcgaa gatcaagacg ttcgttgact tcctgacttc gcatctgggt gcaacgctcg acacccgtct tttcaacgag cactccaatc aaactttcaa tcgtctcgcg cgcgtagtgg agaatgcata aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a