SSGCID: Target Page

BuphA.12800.w

Transcriptional regulator, LysR family

CENTER ID:	BuphA.12800.w
ORGANISM:	Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuphA.12800.w.A1.GE32176	Full length( BuphA.12800.w )		1	316

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|391038.7.peg.5858
RefSeq:	YP_001861687.1
UniProt:	B2JUB9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLDATSVTSA FISSDMDRLQ AMTTFVTVVE MEGFASAARK LNVSPSVISR VMTELEEHLG VRLLTRTTRV VRMTDAGATF FEHCRRILAD VEAAELSAVG VNTTPRGQLT VTAPVLFGKM YVTPIAQDYL TRYPAVSLNC WFMDRVVNLV DEGADVAIRI GELPNSSLQA IAVGHVRRVL CASPGYLETH GVPQHPDDLA SHIAIQATGL TPAPEWRFHV DAKPLTVPIQ PRLVTTTNDS AIAAAIAGLG LVRVLSYQIA EELANGTLRI VLADCEQATL PVHVMHREGK HANQKVRAFL DLAIETLRAK ASLWLA

NT Sequence

ATGCTTGACG CCACCTCCGT GACATCGGCG TTCATCTCTT CAGACATGGA CCGGCTTCAA GCAATGACCA CGTTCGTGAC CGTGGTCGAG ATGGAAGGCT TCGCGTCTGC GGCGCGCAAG CTCAACGTGT CGCCATCGGT CATCAGCCGG GTGATGACTG AGCTCGAAGA ACATCTGGGC GTGCGCCTGT TGACGCGTAC GACGCGCGTC GTGCGCATGA CCGATGCGGG CGCGACGTTC TTCGAACATT GCCGCCGCAT CCTCGCGGAC GTCGAAGCCG CCGAGCTTTC CGCTGTCGGC GTCAACACCA CGCCGCGCGG CCAGTTGACG GTGACAGCGC CCGTCCTGTT CGGCAAGATG TATGTCACGC CCATCGCTCA GGACTATCTG ACGCGCTATC CCGCCGTCAG TCTGAACTGC TGGTTCATGG ACCGCGTCGT GAATCTCGTC GACGAAGGCG CCGATGTGGC GATACGCATC GGTGAGTTGC CGAATTCGTC GTTGCAGGCA ATCGCCGTGG GCCATGTTCG GCGTGTGCTG TGCGCGTCAC CCGGGTATCT GGAAACGCAC GGCGTGCCGC AACATCCCGA CGACCTCGCG TCGCATATTG CCATTCAGGC GACGGGCCTC ACGCCTGCGC CGGAATGGCG CTTTCACGTG GACGCCAAAC CGCTGACCGT GCCGATCCAG CCGCGCCTCG TGACCACCAC CAACGACTCG GCGATCGCGG CGGCAATCGC GGGCCTGGGC CTGGTGCGAG TGCTGTCCTA TCAGATCGCG GAGGAACTGG CGAACGGCAC CTTGCGAATC GTGCTCGCCG ACTGCGAGCA AGCGACGCTG CCCGTTCATG TAATGCATCG CGAGGGCAAG CACGCGAACC AGAAGGTGCG CGCGTTCCTC GATCTCGCGA TCGAAACGCT CAGGGCAAAG GCTTCGCTGT GGCTTGCA

Details for BuphA.12800.w.A1.GE32176

HARVESTED ON:	4/26/2011
SEQUENCED ON:	5/2/2011
EXPECTED MW:	36kDa
OBSERVED MW:	36kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MGTLEAQTQG PGSMLDATSV TSAFISSDMD RLQAMTTFVT VVEMEGFASA ARKLNVSPSV ISRVMTELEE HLGVRLLTRT TRVVRMTDAG ATFFEHCRRI LADVEAAELS AVGVNTTPRG QLTVTAPVLF GKMYVTPIAQ DYLTRYPAVS LNCWFMDRVV NLVDEGADVA IRIGELPNSS LQAIAVGHVR RVLCASPGYL ETHGVPQHPD DLASHIAIQA TGLTPAPEWR FHVDAKPLTV PIQPRLVTTT NDSAIAAAIA GLGLVRVLSY QIAEELANGT LRIVLADCEQ ATLPVHVMHR EGKHANQKVR AFLDLAIETL RAKASLWLA

Validated NT Sequence

ttattatgca agccacagcg aagcctttgc cctgagcgtt tcgatcgcga gatcgaggaa cgcgcgcacc ttctggttcg cgtgcttgcc ctcgcgatgc attacatgaa cgggcagcgt cgcttgctcg cagtcggcga gcacgattcg caaggtgccg ttcgccagtt cctccgcgat ctgataggac agcactcgca ccaggcccag gcccgcgatt gccgccgcga tcgccgagtc gttggtggtg gtcacgaggc gcggctggat cggcacggtc agcggtttgg cgtccacgtg aaagcgccat tccggcgcag gcgtgaggcc cgtcgcctga atggcaatat gcgacgcgag gtcgtcggga tgttgcggca cgccgtgcgt ttccagatac ccgggtgacg cgcacagcac acgccgaaca tggcccacgg cgattgcctg caacgacgaa ttcggcaact caccgatgcg tatcgccaca tcggcgcctt cgtcgacgag attcacgacg cggtccatga accagcagtt cagactgacg gcgggatagc gcgtcagata gtcctgagcg atgggcgtga catacatctt gccgaacagg acgggcgctg tcaccgtcaa ctggccgcgc ggcgtggtgt tgacgccgac agcggaaagc tcggcggctt cgacgtccgc gaggatgcgg cggcaatgtt cgaagaacgt cgcgcccgca tcggtcatgc gcacgacgcg cgtcgtacgc gtcaacaggc gcacgcccag atgttcttcg agctcagtca tcacccggct gatgaccgat ggcgacacgt tgagcttgcg cgccgcagac gcgaagcctt ccatctcgac cacggtcacg aacgtggtca ttgcttgaag ccggtccatg tctgaagaga tgaacgccga tgtcacggag gtggcgtcaa gcatcgaacc aggaccctgg gtctgagctt ccagggtacc catatgatgg tgatggtg

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMLDATSVTS AFISSDMDRL QAMTTFVTVV EMEGFASAAR KLNVSPSVIS RVMTELEEHL GVRLLTRTTR VVRMTDAGAT FFEHCRRILA DVEAAELSAV GVNTTPRGQL TVTAPVLFGK MYVTPIAQDY LTRYPAVSLN CWFMDRVVNL VDEGADVAIR IGELPNSSLQ AIAVGHVRRV LCASPGYLET HGVPQHPDDL ASHIAIQATG LTPAPEWRFH VDAKPLTVPI QPRLVTTTND SAIAAAIAGL GLVRVLSYQI AEELANGTLR IVLADCEQAT LPVHVMHREG KHANQKVRAF LDLAIETLRA KASLWLA

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcttgacgcc acctccgtga catcggcgtt catctcttca gacatggacc ggcttcaagc aatgaccacg ttcgtgaccg tggtcgagat ggaaggcttc gcgtctgcgg cgcgcaagct caacgtgtcg ccatcggtca tcagccgggt gatgactgag ctcgaagaac atctgggcgt gcgcctgttg acgcgtacga cgcgcgtcgt gcgcatgacc gatgcgggcg cgacgttctt cgaacattgc cgccgcatcc tcgcggacgt cgaagccgcc gagctttccg ctgtcggcgt caacaccacg ccgcgcggcc agttgacggt gacagcgccc gtcctgttcg gcaagatgta tgtcacgccc atcgctcagg actatctgac gcgctatccc gccgtcagtc tgaactgctg gttcatggac cgcgtcgtga atctcgtcga cgaaggcgcc gatgtggcga tacgcatcgg tgagttgccg aattcgtcgt tgcaggcaat cgccgtgggc catgttcggc gtgtgctgtg cgcgtcaccc gggtatctgg aaacgcacgg cgtgccgcaa catcccgacg acctcgcgtc gcatattgcc attcaggcga cgggcctcac gcctgcgccg gaatggcgct ttcacgtgga cgccaaaccg ctgaccgtgc cgatccagcc gcgcctcgtg accaccacca acgactcggc gatcgcggcg gcaatcgcgg gcctgggcct ggtgcgagtg ctgtcctatc agatcgcgga ggaactggcg aacggcacct tgcgaatcgt gctcgccgac tgcgagcaag cgacgctgcc cgttcatgta atgcatcgcg agggcaagca cgcgaaccag aaggtgcgcg cgttcctcga tctcgcgatc gaaacgctca gggcaaaggc ttcgctgtgg cttgcataaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa