SSGCID: Target Page

BuphA.12800.v

Transcriptional regulator, LysR family

CENTER ID:	BuphA.12800.v
ORGANISM:	Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuphA.12800.v.A2.GE34731	Mature protein( BuphA.12800.v )		37	303

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|391038.7.peg.7400
RefSeq:	YP_001863147.1
UniProt:	B2JU48

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MQRQFEDLLL GSIELFCLAA ELESFTMAAA AASVTPAAVS RSVARLEERL GVRLFVRTTR QIRLTDAGRR YFEQCRLALN QLADAEREAT GQQSTPSGVL RISMPTPYSH YRVLPLLAEF RALYPEVTVE THLSNRNIDF ADEGFDLAIR GRAPDDSALI ARKLEDAELV VVASPAYLKS AGKLDTPDDL IHHECIQFAL PSTGRNIPWI FRHEDAEIDM MTRGGYGTSG DVLAGATLAR HGAGLFQTYR FVVDKDLKDG TLREVLQPYG GRTRPFVLLY PHARHLSSRV RCFVEFLVEK LRA

NT Sequence

ATGCAGCGTC AATTCGAAGA CCTACTCCTT GGCAGCATCG AACTGTTCTG CCTCGCAGCC GAACTGGAGA GCTTCACGAT GGCAGCAGCG GCTGCGAGCG TGACGCCCGC GGCCGTCAGC CGTTCGGTTG CGCGTCTGGA GGAAAGGCTC GGCGTCCGGC TTTTCGTGCG CACCACACGG CAAATCAGGC TCACCGATGC AGGACGCCGC TACTTCGAGC AATGCCGTCT CGCGCTCAAC CAGCTCGCCG ATGCGGAACG CGAAGCGACG GGCCAGCAGA GCACGCCGTC GGGCGTGCTG CGCATCAGCA TGCCGACGCC GTACAGCCAC TATCGTGTGC TGCCCCTGCT CGCGGAATTT CGCGCACTGT ATCCGGAAGT GACCGTCGAA ACACATCTCA GCAATCGCAA CATCGATTTC GCGGACGAAG GCTTCGACCT CGCCATTCGA GGCCGCGCGC CCGACGACTC CGCCCTCATC GCCCGTAAGC TCGAAGACGC CGAACTCGTG GTGGTCGCTT CGCCCGCGTA TCTGAAGTCG GCGGGCAAGC TGGACACACC CGACGACCTG ATTCATCACG AATGCATTCA GTTCGCGCTG CCGAGCACGG GCCGCAACAT CCCGTGGATC TTCCGGCACG AAGATGCAGA AATCGACATG ATGACGCGCG GTGGGTATGG CACGTCCGGC GACGTGCTGG CGGGCGCGAC GCTCGCGCGA CACGGCGCTG GGCTGTTCCA GACCTATCGC TTCGTCGTCG ACAAGGATCT GAAGGACGGC ACGCTGCGCG AAGTGCTCCA ACCGTACGGC GGACGCACGC GGCCCTTCGT GCTGCTCTAT CCGCATGCGC GGCATCTGTC GTCGCGCGTG CGCTGCTTCG TCGAGTTTCT GGTGGAAAAG CTCAGAGCG

Details for BuphA.12800.v.A2.GE34731

HARVESTED ON:	3/13/2012
SEQUENCED ON:	3/12/2012
EXPECTED MW:	32kDa
OBSERVED MW:	32kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MGTLEAQTQG PGSMAAVSRS VARLEERLGV RLFVRTTRQI RLTDAGRRYF EQCRLALNQL ADAEREATGQ QSTPSGVLRI SMPTPYSHYR VLPLLAEFRA LYPEVTVETH LSNRNIDFAD EGFDLAIRGR APDDSALIAR KLEDAELVVV ASPAYLKSAG KLDTPDDLIH HECIQFALPS TGRNIPWIFR HEDAEIDMMT RGGYGTSGDV LAGATLARHG AGLFQTYRFV VDKDLKDGTL REVLQPYGGR TRPFVLLYPH ARHLSSRVRC FVEFLVEKLR A

Validated NT Sequence

ttattacgct ctgagctttt ccaccagaaa ctcgacgaag cagcgcacgc gcgacgacag atgccgcgca tgcggataga gcagcacgaa gggccgcgtg cgtccgccgt acggttggag cacttcgcgc agcgtgccgt ccttcagatc cttgtcgacg acgaagcgat aggtctggaa cagcccagcg ccgtgtcgcg cgagcgtcgc gcccgccagc acgtcgccgg acgtgccata cccaccgcgc gtcatcatgt cgatttctgc atcttcgtgc cggaagatcc acgggatgtt gcggcccgtg ctcggcagcg cgaactgaat gcattcgtga tgaatcaggt cgtcgggtgt gtccagcttg cccgccgact tcagatacgc gggcgaagcg accaccacga gttcggcgtc ttcgagctta cgggcgatga gggcggagtc gtcgggcgcg cggcctcgaa tggcgaggtc gaagccttcg tccgcgaaat cgatgttgcg attgctgaga tgtgtttcga cggtcacttc cggatacagt gcgcgaaatt ccgcgagcag gggcagcaca cgatagtggc tgtacggcgt cggcatgctg atgcgcagca cgcccgacgg cgtgctctgc tggcccgtcg cttcgcgttc cgcatcggcg agctggttga gcgcgagacg gcattgctcg aagtagcggc gtcctgcatc ggtgagcctg atttgccgtg tggtgcgcac gaaaagccgg acgccgagcc tttcctccag acgcgcaacc gaacggctga cggccgccat cgaaccagga ccctgggtct gagcttccag ggtacccata tgatgg

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMAAVSRSVA RLEERLGVRL FVRTTRQIRL TDAGRRYFEQ CRLALNQLAD AEREATGQQS TPSGVLRISM PTPYSHYRVL PLLAEFRALY PEVTVETHLS NRNIDFADEG FDLAIRGRAP DDSALIARKL EDAELVVVAS PAYLKSAGKL DTPDDLIHHE CIQFALPSTG RNIPWIFRHE DAEIDMMTRG GYGTSGDVLA GATLARHGAG LFQTYRFVVD KDLKDGTLRE VLQPYGGRTR PFVLLYPHAR HLSSRVRCFV EFLVEKLRA

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcggccgtc agccgttcgg ttgcgcgtct ggaggaaagg ctcggcgtcc ggcttttcgt gcgcaccaca cggcaaatca ggctcaccga tgcaggacgc cgctacttcg agcaatgccg tctcgcgctc aaccagctcg ccgatgcgga acgcgaagcg acgggccagc agagcacgcc gtcgggcgtg ctgcgcatca gcatgccgac gccgtacagc cactatcgtg tgctgcccct gctcgcggaa tttcgcgcac tgtatccgga agtgaccgtc gaaacacatc tcagcaatcg caacatcgat ttcgcggacg aaggcttcga cctcgccatt cgaggccgcg cgcccgacga ctccgccctc atcgcccgta agctcgaaga cgccgaactc gtggtggtcg cttcgcccgc gtatctgaag tcggcgggca agctggacac acccgacgac ctgattcatc acgaatgcat tcagttcgcg ctgccgagca cgggccgcaa catcccgtgg atcttccggc acgaagatgc agaaatcgac atgatgacgc gcggtgggta tggcacgtcc ggcgacgtgc tggcgggcgc gacgctcgcg cgacacggcg ctgggctgtt ccagacctat cgcttcgtcg tcgacaagga tctgaaggac ggcacgctgc gcgaagtgct ccaaccgtac ggcggacgca cgcggccctt cgtgctgctc tatccgcatg cgcggcatct gtcgtcgcgc gtgcgctgct tcgtcgagtt tctggtggaa aagctcagag cgtaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa