BuphA.12800.u

Transcriptional regulator, LysR family

CENTER ID: BuphA.12800.u
ORGANISM: Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuphA.12800.u.A1.GE34733 Full length( BuphA.12800.u ) 1 295
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|391038.7.peg.7386
RefSeq: YP_001863133.1
UniProt: B2JU34

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDRFEAMSVY VRVVEAGSLS AAARAIPMSL TSVSRHIAAL EDQFGAQLLR RTTRNLAMTD EGRILYDRAK SILGEVDELG SALSAGRGRL SGRLRIAAPN LLGRLLIAPL LPRFLAMHPD VAVDLMLVDR VVNIVEEGLH LAVRVGRLPD SSLVARKLDD IEMMVCAAPS YLARRGTPRR PDDLRRHDCL VFSDAAGPVD WRFQSGATRT RVSVTGRLWM NSLDALVSAA LEGAGIVRAP AWQVAAHIDA GRLQPVLDKF APPATPVHVL FERTKLASPK IRTFVDYLVD QWGRV
NT Sequence
ATGGACCGTT TTGAAGCAAT GAGCGTTTAC GTCCGGGTCG TGGAGGCGGG AAGCCTGTCC GCGGCGGCGC GGGCAATCCC AATGTCGTTG ACTTCGGTCA GCAGGCATAT CGCGGCGCTT GAAGATCAGT TCGGCGCGCA ACTGCTTCGG CGCACCACTC GCAATCTGGC GATGACGGAC GAAGGACGCA TCCTCTACGA TCGCGCGAAG TCGATTCTCG GCGAAGTGGA TGAACTCGGC AGCGCGCTGT CGGCGGGGCG GGGAAGGCTG TCCGGGCGGC TGCGGATCGC CGCGCCGAAT CTGCTGGGAC GGCTGCTGAT CGCGCCGCTG CTGCCGCGCT TTCTCGCGAT GCATCCGGAC GTGGCCGTGG ATCTCATGCT CGTCGATCGC GTCGTCAATA TCGTCGAGGA AGGCTTGCAT CTTGCCGTAC GCGTCGGCCG TCTGCCGGAT TCGAGCCTCG TCGCGCGCAA GCTCGACGAC ATCGAGATGA TGGTGTGCGC CGCGCCGTCG TATCTGGCGC GGCGTGGCAC GCCTCGCAGG CCCGACGATC TGCGCCGGCA CGATTGCCTG GTATTCTCGG ATGCGGCCGG GCCTGTCGAC TGGCGCTTTC AGTCCGGTGC GACGCGCACG CGCGTGAGTG TGACAGGGCG TCTGTGGATG AATAGCCTCG ACGCGCTGGT GTCCGCCGCG CTCGAAGGCG CGGGCATCGT GCGTGCGCCG GCGTGGCAGG TCGCCGCGCA TATCGACGCG GGGCGCCTGC AGCCCGTGCT CGACAAGTTT GCGCCGCCCG CGACGCCTGT GCATGTGCTG TTCGAACGCA CGAAGCTCGC GTCGCCGAAG ATCAGGACGT TCGTCGACTA TCTCGTCGAT CAATGGGGCC GGGTG
Details for BuphA.12800.u.A1.GE34733
HARVESTED ON: 3/13/2012
SEQUENCED ON: 3/12/2012
EXPECTED MW: 34kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 22
PERCENT COVERAGE: 88
Validated AA Sequence
MHRRRGRRKL VEHGLQAPRV DMRGDLPRRR THDARAFERG GHQRVEAIHP QTPCHTHARA RRTGLKAPVD RPGRIREYQA IVPAQIVGPA RRATPRQIRR RGAHHHLDVV ELARDEARIR QTADAYGKMQ AFLDDIDDAI DEHEIHGHVR MHREKARQQR RDQQPSQQIR RGDPQPPGQP SPPRRQRAAE FIHFAENRLR AIVEDASFVR HRQIASGAPK QLRAELIFKR RDMPADRSQR HWGLPAPPRT GFPPPRPGRK RSLLQNGPSN QDPGSELPGY
Validated NT Sequence
ttattacacc cggccccatt gatcgacgag atagtcgacg aacgtcctga tcttcggcga cgcgagcttc gtgcgttcga acagcacatg cacaggcgtc gcgggcggcg caaacttgtc gagcacgggc tgcaggcgcc ccgcgtcgat atgcgcggcg acctgccacg ccggcgcacg cacgatgccc gcgccttcga gcgcggcgga caccagcgcg tcgaggctat tcatccacag acgccctgtc acactcacgc gcgtgcgcgt cgcaccggac tgaaagcgcc agtcgacagg cccggccgca tccgagaata ccaggcaatc gtgccggcgc agatcgtcgg gcctgcgagg cgtgccacgc cgcgccagat acgacggcgc ggcgcacacc atcatctcga tgtcgtcgag cttgcgcgcg acgaggctcg aatccggcag acggccgacg cgtacggcaa gatgcaagcc ttcctcgacg atattgacga cgcgatcgac gagcatgaga tccacggcca cgtccggatg catcgcgaga aagcgcggca gcagcggcgc gatcagcagc cgtcccagca gattcggcgc ggcgatccgc agccgcccgg acagccttcc ccgccccgcc gacagcgcgc tgccgagttc atccacttcg ccgagaatcg acttcgcgcg atcgtagagg atgcgtcctt cgtccgtcat cgccagattg cgagtggtgc gccgaagcag ttgcgcgccg aactgatctt caagcgccgc gatatgcctg ctgaccgaag tcaacgacat tggggattgc ccgcgccgcc gcggacaggc ttcccgcctc cacgacccgg acgtaaacgc tcattgcttc aaaacggtcc atcgaaccag gaccctgggt ctgagcttcc agggtacc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDRFEAMSV YVRVVEAGSL SAAARAIPMS LTSVSRHIAA LEDQFGAQLL RRTTRNLAMT DEGRILYDRA KSILGEVDEL GSALSAGRGR LSGRLRIAAP NLLGRLLIAP LLPRFLAMHP DVAVDLMLVD RVVNIVEEGL HLAVRVGRLP DSSLVARKLD DIEMMVCAAP SYLARRGTPR RPDDLRRHDC LVFSDAAGPV DWRFQSGATR TRVSVTGRLW MNSLDALVSA ALEGAGIVRA PAWQVAAHID AGRLQPVLDK FAPPATPVHV LFERTKLASP KIRTFVDYLV DQWGRV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggaccgtttt gaagcaatga gcgtttacgt ccgggtcgtg gaggcgggaa gcctgtccgc ggcggcgcgg gcaatcccaa tgtcgttgac ttcggtcagc aggcatatcg cggcgcttga agatcagttc ggcgcgcaac tgcttcggcg caccactcgc aatctggcga tgacggacga aggacgcatc ctctacgatc gcgcgaagtc gattctcggc gaagtggatg aactcggcag cgcgctgtcg gcggggcggg gaaggctgtc cgggcggctg cggatcgccg cgccgaatct gctgggacgg ctgctgatcg cgccgctgct gccgcgcttt ctcgcgatgc atccggacgt ggccgtggat ctcatgctcg tcgatcgcgt cgtcaatatc gtcgaggaag gcttgcatct tgccgtacgc gtcggccgtc tgccggattc gagcctcgtc gcgcgcaagc tcgacgacat cgagatgatg gtgtgcgccg cgccgtcgta tctggcgcgg cgtggcacgc ctcgcaggcc cgacgatctg cgccggcacg attgcctggt attctcggat gcggccgggc ctgtcgactg gcgctttcag tccggtgcga cgcgcacgcg cgtgagtgtg acagggcgtc tgtggatgaa tagcctcgac gcgctggtgt ccgccgcgct cgaaggcgcg ggcatcgtgc gtgcgccggc gtggcaggtc gccgcgcata tcgacgcggg gcgcctgcag cccgtgctcg acaagtttgc gccgcccgcg acgcctgtgc atgtgctgtt cgaacgcacg aagctcgcgt cgccgaagat caggacgttc gtcgactatc tcgtcgatca atggggccgg gtgtaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa