BuphA.12800.t

Transcriptional regulator, LysR family

CENTER ID: BuphA.12800.t
ORGANISM: Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuphA.12800.t.A1.GE32407 Full length( BuphA.12800.t ) 1 338
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|391038.7.peg.6978
RefSeq: YP_001862712.1
UniProt: B2JSW3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSDYAPRRSV TGADDPIAST FATSFAGVVS FIAVANEGSF ARAGDRLGIG RSSVSRNVQK LEAQLDTRLF LRTTRSTSLT REGELFFENC QAGVERIVQA LEEMRELRSG PPRGRLRIYS TPGFGRKIVA PLLRGFHAQY PDIALELLLN DRPADFTADR IDVSFREGRM EDSEIVARQL IPMQMIVCAA PSYVQAHGLP RHVDELAHHG CINFRTASGR LKEWEFRVDG LPLRHHPHAS HTFNDPDLIL QSVLDGQGLA QLPGYQVCDL LGEGRLVSCL TQYAPDDSGH YICYLSRKHL PARIRVFVDY MTEHTRALDL HRLTTLDALS TENESVSV
NT Sequence
ATGTCAGATT ACGCACCGCG CCGCTCCGTC ACTGGCGCCG ACGATCCGAT TGCGAGCACG TTCGCAACGA GTTTCGCCGG CGTCGTCTCC TTTATTGCCG TTGCCAACGA GGGCAGCTTC GCGAGAGCGG GCGATCGCCT CGGCATCGGG CGCTCGTCCG TCAGCCGGAA CGTGCAGAAG CTCGAGGCGC AGCTCGATAC GCGCCTCTTT CTGCGCACCA CCCGCAGCAC GTCGCTGACA CGCGAGGGCG AACTGTTCTT TGAGAATTGC CAGGCGGGCG TGGAGCGCAT CGTCCAGGCG CTCGAAGAAA TGCGTGAACT GCGCAGCGGT CCGCCCCGTG GACGACTGCG CATTTACTCG ACCCCAGGCT TCGGGCGCAA GATCGTGGCG CCGCTGCTGC GCGGCTTCCA CGCGCAGTAT CCCGACATCG CACTCGAGCT GCTGCTCAAC GATCGTCCCG CGGACTTCAC CGCCGACCGT ATCGACGTGT CGTTTCGCGA AGGACGCATG GAAGACAGCG AGATCGTCGC GCGTCAACTG ATACCGATGC AGATGATCGT GTGCGCAGCG CCATCGTACG TTCAGGCGCA TGGCTTGCCG CGCCACGTCG ACGAACTTGC CCATCATGGC TGCATCAATT TCCGCACGGC GTCGGGACGC TTGAAGGAAT GGGAGTTCAG GGTCGATGGA CTGCCGCTGA GGCACCATCC ACACGCCTCA CACACGTTCA ACGATCCCGA TCTGATCTTG CAGTCGGTGC TCGATGGACA AGGCCTCGCG CAATTGCCGG GCTACCAGGT GTGCGATCTG CTGGGCGAGG GACGGCTCGT GAGTTGTCTC ACGCAGTATG CGCCCGACGA CAGCGGCCAC TACATCTGCT ATCTGAGCCG CAAGCATTTG CCCGCGCGCA TTCGCGTGTT CGTCGACTAT ATGACTGAGC ACACGCGAGC GCTCGATCTG CATCGCCTGA CTACGCTTGA CGCTTTATCT ACGGAAAACG AGAGCGTATC AGTC
Details for BuphA.12800.t.A1.GE32407
HARVESTED ON: 11/8/2011
SEQUENCED ON: 11/11/2011
EXPECTED MW: 40kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 96
PERCENT COVERAGE: 82
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSDYAPRRS VTGADDPIAS TFATSFAGVV SFIAVANEGS FARAGDRLGI GRSSVSRNVQ KLEAQLDTRL FLRTTRSTSL TREGELFFEN CQAGVERIVQ ALEEMRELRS GPPRGRLRIY STPGFGRKIV APLLRGFHAQ YPDIALELLL NDRPADFTAD RIDVSFREGR MEDSEIVARQ LIPMQMIVCA APSYVQAHGL PRHVDELAHH GCINFRTASG RLKEWEFRVD GLPLRHHPHA SHTFNDPDLI LQSVLDGQGL AQLPGYXXAI CWARXGS
Validated NT Sequence
ttttgtttnn ctttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgtcag attacgcacc gcgccgctcc gtcactggcg ccgacgatcc gattgcgagc acgttcgcaa cgagtttcgc cggcgtcgtc tcctttattg ccgttgccaa cgagggcagc ttcgcgagag cgggcgatcg cctcggcatc gggcgctcgt ccgtcagccg gaacgtgcag aagctcgagg cgcagctcga tacgcgcctc tttctgcgca ccacccgcag cacgtcgctg acacgcgagg gcgaactgtt ctttgagaat tgccaggcgg gcgtggagcg catcgtccag gcgctcgaag aaatgcgtga actgcgcagc ggtccgcccc gtggacgact gcgcatttac tcgaccccag gcttcgggcg caagatcgtg gcgccgctgc tgcgcggctt ccacgcgcag tatcccgaca tcgcactcga gctgctgctc aacgatcgtc ccgcggactt caccgccgac cgtatcgacg tgtcgtttcg cgaaggacgc atggaagaca gcgagatcgt cgcgcgtcaa ctgataccga tgcagatgat cgtgtgcgca gcgccatcgt acgttcaggc gcatggcttg ccgcgccacg tcgacgaact tgcccatcat ggctgcatca atttccgcac ggcgtcngga cgcttgaagg aatgggagtt cagggtcgat ggactgccgc tgaggcacca tccacacgcc tcacacacgt tcaacgatcc cgatctgatc ttgcagtcgg tgctcgatgg acaaggnctc gcgcaattgc cgggctaccn nngtgcgatc tgctgggcga ggganggctc gtgagttgtc tcacgcagta tgcgcccgac gacngcggcc actacatctg ctatctganc cgcnagcatt tgcccgngnn cattcncgtg ntcgtcgact atatgactga gcacacgcga gcnctcgatc tgcatcnnng actacnnttg nngctttatc tacggaaann anngcgtatc antctnnnna acnnnncnan nnantgcann nnnnnnnnnn ngnnnnnnnn nannnnnnan nnannnnntg nnnnnnnnnn nnnnnnnnan nnannanann nnnnnnnnnn nnnnnnnnnn nnnnng
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSDYAPRRS VTGADDPIAS TFATSFAGVV SFIAVANEGS FARAGDRLGI GRSSVSRNVQ KLEAQLDTRL FLRTTRSTSL TREGELFFEN CQAGVERIVQ ALEEMRELRS GPPRGRLRIY STPGFGRKIV APLLRGFHAQ YPDIALELLL NDRPADFTAD RIDVSFREGR MEDSEIVARQ LIPMQMIVCA APSYVQAHGL PRHVDELAHH GCINFRTASG RLKEWEFRVD GLPLRHHPHA SHTFNDPDLI LQSVLDGQGL AQLPGYQVCD LLGEGRLVSC LTQYAPDDSG HYICYLSRKH LPARIRVFVD YMTEHTRALD LHRLTTLDAL STENESVSV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcagattac gcaccgcgcc gctccgtcac tggcgccgac gatccgattg cgagcacgtt cgcaacgagt ttcgccggcg tcgtctcctt tattgccgtt gccaacgagg gcagcttcgc gagagcgggc gatcgcctcg gcatcgggcg ctcgtccgtc agccggaacg tgcagaagct cgaggcgcag ctcgatacgc gcctctttct gcgcaccacc cgcagcacgt cgctgacacg cgagggcgaa ctgttctttg agaattgcca ggcgggcgtg gagcgcatcg tccaggcgct cgaagaaatg cgtgaactgc gcagcggtcc gccccgtgga cgactgcgca tttactcgac cccaggcttc gggcgcaaga tcgtggcgcc gctgctgcgc ggcttccacg cgcagtatcc cgacatcgca ctcgagctgc tgctcaacga tcgtcccgcg gacttcaccg ccgaccgtat cgacgtgtcg tttcgcgaag gacgcatgga agacagcgag atcgtcgcgc gtcaactgat accgatgcag atgatcgtgt gcgcagcgcc atcgtacgtt caggcgcatg gcttgccgcg ccacgtcgac gaacttgccc atcatggctg catcaatttc cgcacggcgt cgggacgctt gaaggaatgg gagttcaggg tcgatggact gccgctgagg caccatccac acgcctcaca cacgttcaac gatcccgatc tgatcttgca gtcggtgctc gatggacaag gcctcgcgca attgccgggc taccaggtgt gcgatctgct gggcgaggga cggctcgtga gttgtctcac gcagtatgcg cccgacgaca gcggccacta catctgctat ctgagccgca agcatttgcc cgcgcgcatt cgcgtgttcg tcgactatat gactgagcac acgcgagcgc tcgatctgca tcgcctgact acgcttgacg ctttatctac ggaaaacgag agcgtatcag tctaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa